Status
Please wait ...
Gene information
General information
TC009947 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11443754..11445779
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009947
    iBeetle screen iB_04731

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009947 RA
      MVLVISQQT FDEAVQENM NDLGLSQEE AVAEAIAQF 
      ESQGVDLSQ IITDPESFR NLSATIKKL AEFQDKRLT 
      PECIELLHA LKKECDKGL PHRVQAGKD GAYNVILDI 
      LLKSHAQKD ITEAAIKAM VSLMTKQPD LLDERGVLV 
      ILSNLDKGE PETQRFVLK WCKECCVMH EMNRQKLIE 
      LNIVNKLSK LLKEGNSHI LRDTLLVLR ALTLDDDVR 
      VEFGRAHEH ARIIATETL TPLIGLIQK FQAEDILVN 
      DLILTLSAL LVRNEFCKQ VDEVGGIEL ISEVVTAFL 
      NNDKVVRQC FKFLKALAG NDECKVHII QKGLAPIIS 
      DALNAHQDN VPTAVGGLS CVAALCLRS PDNSKALFE 
      AALPEVIIA IMKKHPNDK LVQKTASWA IRNMVSRSR 
      YQNGHFLEL GVEELLKNN LVKFKEFEY DTKAALRDL 
      EVKVDLKEE WTGKGGKIT SGTSLSMDR K
    CDS sequence
    • >TC009947 RA
      atggtattg gtaatttcg caacaaacg ttcgacgaa 
      gccgtccaa gaaaatatg aacgacctt ggattatct 
      caagaggag gctgttgct gaagccatc gcccaattt 
      gagtcccaa ggagttgat ctgagtcaa attatcacc 
      gaccctgag tcttttcga aacctatcg gccactatc 
      aaaaagctg gcagagttc caggacaaa aggctgacc 
      cctgagtgt attgaactc ttacacgcc ttaaaaaaa 
      gaatgtgat aaggggctc ccacacaga gtccaagct 
      ggcaaggac ggtgcttac aatgtgata ctcgacatt 
      cttctaaaa tctcacgct caaaaagac ataacggag 
      gctgcaatc aaagccatg gtgtcgctg atgaccaaa 
      caacccgat cttcttgac gagagaggt gttttggtc 
      attttgagc aacctggac aaaggcgaa ccagaaact 
      caaagattt gtcttaaag tggtgcaaa gaatgctgt 
      gtgatgcat gaaatgaac agacaaaaa ttaattgag 
      ctaaacatc gtaaataaa ctgtccaag cttttgaag 
      gaaggaaat tcgcatatt ttgagagat actctcctc 
      gttttaaga gccttgact ttggatgac gacgtccgt 
      gtagagttc ggacgtgcc cacgagcat gcacgtatt 
      atagccaca gagacgcta acccctctc ataggattg 
      attcaaaaa ttccaggct gaagatatt ctcgttaat 
      gatttaatt ctcactctg tccgctttg cttgtgcga 
      aacgaattc tgcaagcaa gtggatgaa gtcggcggg 
      attgaacta atcagtgaa gttgtgact gcttttctc 
      aacaacgat aaagttgtg agacaatgc ttcaaattc 
      ctcaaggcc ttggctgga aacgacgag tgcaaagtg 
      cacataatc caaaagggc ttggccccg atcatttca 
      gacgcgctg aacgcccac caggataac gtcccgaca 
      gcagtcggg ggtctgagt tgcgtggca gcgctgtgt 
      ctcaggtcc cccgataac agcaaagcg ttgtttgag 
      gcagcctta cccgaagtt ataatcgcg ataatgaaa 
      aaacacccc aatgataaa ttggttcag aaaacggca 
      agttgggca attcgtaac atggtctcg cgcagtcgc 
      taccaaaat ggccatttc ctagagctg ggtgttgaa 
      gaattgctt aagaacaat ttggtgaaa tttaaggag 
      tttgagtac gacaccaag gcggcttta agagacttg 
      gaagttaag gtggacctg aaggaggag tggaccgga 
      aaaggcggg aaaattact tccgggacc agcttatcc 
      atggataga aaataa
    mRNA sequence
    • >TC009947 RA
      tgttggcaa taatcgccc atctgtcaa tttttgggg 
      cacctgtgt cgggcacgc gcatagacc cagtggcac 
      gaaacactg ttcactttt ccaatcaaa aatatattt 
      tgcacccga aatacaaaa ttttcagtt acttgaatc 
      aagaagtgg cttcacttt accgatctc aacacgcgg 
      gtttaattt tttggtgga atcactagc actaaattt 
      tgaattaac atttcctaa aatggtatt ggtaatttc 
      gcaacaaac gttcgacga agccgtcca agaaaatat 
      gaacgacct tggattatc tcaagagga ggctgttgc 
      tgaagccat cgcccaatt tgagtccca aggagttga 
      tctgagtca aattatcac cgaccctga gtcttttcg 
      aaacctatc ggccactat caaaaagct ggcagagtt 
      ccaggacaa aaggctgac ccctgagtg tattgaact 
      cttacacgc cttaaaaaa agaatgtga taaggggct 
      cccacacag agtccaagc tggcaagga cggtgctta 
      caatgtgat actcgacat tcttctaaa atctcacgc 
      tcaaaaaga cataacgga ggctgcaat caaagccat 
      ggtgtcgct gatgaccaa acaacccga tcttcttga 
      cgagagagg tgttttggt cattttgag caacctgga 
      caaaggcga accagaaac tcaaagatt tgtcttaaa 
      gtggtgcaa agaatgctg tgtgatgca tgaaatgaa 
      cagacaaaa attaattga gctaaacat cgtaaataa 
      actgtccaa gcttttgaa ggaaggaaa ttcgcatat 
      tttgagaga tactctcct cgttttaag agccttgac 
      tttggatga cgacgtccg tgtagagtt cggacgtgc 
      ccacgagca tgcacgtat tatagccac agagacgct 
      aacccctct cataggatt gattcaaaa attccaggc 
      tgaagatat tctcgttaa tgatttaat tctcactct 
      gtccgcttt gcttgtgcg aaacgaatt ctgcaagca 
      agtggatga agtcggcgg gattgaact aatcagtga 
      agttgtgac tgcttttct caacaacga taaagttgt 
      gagacaatg cttcaaatt cctcaaggc cttggctgg 
      aaacgacga gtgcaaagt gcacataat ccaaaaggg 
      cttggcccc gatcatttc agacgcgct gaacgccca 
      ccaggataa cgtcccgac agcagtcgg gggtctgag 
      ttgcgtggc agcgctgtg tctcaggtc ccccgataa 
      cagcaaagc gttgtttga ggcagcctt acccgaagt 
      tataatcgc gataatgaa aaaacaccc caatgataa 
      attggttca gaaaacggc aagttgggc aattcgtaa 
      catggtctc gcgcagtcg ctaccaaaa tggccattt 
      cctagagct gggtgttga agaattgct taagaacaa 
      tttggtgaa atttaagga gtttgagta cgacaccaa 
      ggcggcttt aagagactt ggaagttaa ggtggacct 
      gaaggagga gtggaccgg aaaaggcgg gaaaattac 
      ttccgggac cagcttatc catggatag aaaataatt 
      tttgcattt actacacaa taatttatg cacctaatt 
      taagctttg tataacaag actaataaa tttttcaca 
      ttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY