General information | |||
TC009940 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 11515775..11521619 | |
Sequence information | |||
Transcripts / Proteins (4) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009940 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04730 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
thorax musculature partiallydegenerated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen musculature degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head musculature potentiallyrounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax musculature potentiallyrounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
leg musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
labium distal broader - Penetrance (percentage of animals/eggs on slide): more than 80%
maxillary palp broader - Penetrance (percentage of animals/eggs on slide): more than 80%
antenna distal broader - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC009940 RA
MDGQGPEVT FLFQFGLIR DAITVPVAS LNLKTIKDL ACNFINSKI PDHGINRLE DRLLLFRHE YNSNNILQF INSASEIVD ETLIEIVLT GKVSTPEIT IVRPHALSV HSYKTPTFC DFCGEMLFG LVRQGLKCE GCGQNYHKR CVVKVPNNC SYTLLGDKQ RRRSTTLQV PRSPSGGSN SSLASANST QTEDSGLVR LANFFTGGV SLKD
- >TC009940 RB
MSSRVKIPH TFVLHTYTR PTVCQYCKK LLKGLFKQG LQCKDCNYN AHKKCMDKV PKDCTGEVP KENSCNYDY ADSSASNDS QCHLTLVED ENEDEKLNG ANKYIEVIP VHDDDNDSI PSSCSSSSS PSANIPLQR IVQSVKHTK RRGSKVIKE GWLVHFTNK DRMVRRHFW RLDTKSIVL FQNDHTSKY YKEIPLSEI LTIDSARSK QAEVMHCFE IRTANVDYF VGQDPLYDL QDGNNVNLP PPDSGIGAY LAKSWETSI RQALMPVTA GPKPEETNE SEEHIMDMS QIYQIYPDE VLGSGQFGI VYGGIHRKT SRPVAIKVI DKLRFPTKQ EAQLKNEVA ILQNLSHPG VVNLERMFE TPERIFVVM EKLKGDMLE MILSHEKGR LTERVTKFL ITQILIALK HLHSKNIVH CDLKPENVL LSSDAEFPQ VKLCDFGFA RIIGEKSFR RSVVGTPAY LAPEVLRNK GYNRSLDMW SVGVIVYVS LSGTFPFNE DEDINEQIQ NAAFMYPPN PWKEISSDA IDLINNLLQ VKQRKRYTV DKSLQHIWL QDYQTWCDL RALENQIGV RYLTHESDD ARWERYRAE L
- >TC009940 RC
MDGQGPEVT FLFQFGLIR DAITVPVAS LNLKTIKDL ACNFINSKI PDHGINRLE DRLLLFRHE YNSNNILQF INSASEIVD ETLIEIVLT GKVSTPEIT IVRPHALSV HSYKTPTFC DFCGEMLFG LVRQGLKCE GCGQNYHKR CVVKVPNNC SYTLLGDKQ RRRSTTLQV PRSPSGGSN SSLASANST QTEDSGLFQ APNRSPSLG GRPAWVEKE MSSRVKIPH TFVLHTYTR PTVCQYCKK LLKGLFKQG LQCKDCNYN AHKKCMDKV PKDCTGEVP KENSCNYDY ADSSASNDS QCHLTLVED ENEDEKLNG ANKYIEVIP VHDDDNDSI PSSCSSSSS PSANIPLQR IVQSVKHTK RRGSKVIKE GWLVHFTNK DRMVRRHFW RLDTKSIVL FQNDHTSKY YKEIPLSEI LTIDSARSK QAEVMHCFE IRTANVDYF VGQDPLYDL QDGNNVNLP PPDSGIGAY LAKSWETSI RQALMPVTA GPKPEETNE SEEHIMDMS QIYQIYPDE VLGSGQFGI VYGGIHRKT SRPVAIKVI DKLRFPTKQ EAQLKNEVA ILQNLSHPG VVNLERMFE TPERIFVVM EKLKGDMLE MILSHEKGR LTERVTKFL ITQILIALK HLHSKNIVH CDLKPENVL LSSDAEFPQ VKLCDFGFA RIIGEKSFR RSVVGTPAY LAPEVLRNK GYNRSLDMW SVGVIVYVS LSGTFPFNE DEDINEQIQ NAAFMYPPN PWKEISSDA IDLINNLLQ VKQRKRYTV DKSLQHIWL QDYQTWCDL RALENQIGV RYLTHESDD ARWERYRAE L
- >TC009940 RD
MDGQGPEVT FLFQFGLIR DAITVPVAS LNLKTIKDL ACNFINSKI PDHGINRLE DRLLLFRHE YNSNNILQF INSASEIVD ETLIEIVLT GKVVSTPEI TIVRPHALS VHSYKTPTF CDFCGEMLF GLVRQGLKC EGCGQNYHK RCVVKVPNN CSYTLLGDK QRRRSTTLQ VPRSPSGGS NSSLASANS TQTEDSGLV RLANFFTGG VSLKD
- >TC009940 RA
atggatggt caggggcca gaagtgacg ttcctattc caatttggc ttgattcga gatgcaatc acagttcct gtggcatcg ctgaacctc aaaaccatc aaagacctg gcttgcaat tttataaac tcgaaaata cccgaccat gggatcaac aggttggag gaccgcctc ttgctgttc agacacgag tacaactcg aacaacatt cttcagttc atcaactcg gcttcggag atcgtcgac gaaaccctg atcgagatc gtcttgact ggcaaagtt tcaaccccc gaaatcaca atagtcaga ccgcatgct ctctcagtt cactcgtac aaaacacca acgttttgc gatttttgc ggcgaaatg ctgttcggg ttggtgcgc caggggctc aagtgtgaa ggatgtggg caaaattac cacaagaga tgtgtggtc aaagtcccg aacaactgc agctacact ctcttggga gacaagcag aggagacgc tcgaccact ctgcaggtt ccgcggagt ccgtcaggg ggctccaac tcgtcgcta gcgtccgcc aactcgact cagacagag gacagtggc ttggtaagg cttgctaat ttttttacc gggggagtc agtctcaag gactga
- >TC009940 RB
atgtcaagt cgggtaaaa attccgcac acgttcgtc ttgcacact tacacaaga ccgacagtg tgtcagtac tgtaaaaag ctgttgaaa ggactgttc aagcaaggt ctccagtgc aaagactgc aattacaac gcgcacaaa aagtgcatg gacaaagta cctaaagat tgcacgggt gaagtgcca aaggagaac tcctgcaat tacgactac gccgacagc tctgccagt aacgacagc cagtgtcac ctgaccctg gtcgaagac gagaacgaa gacgagaaa ctgaatggt gctaacaaa tatattgaa gttattccg gtgcacgat gacgacaac gactcaatt ccttccagt tgtagctct tcttcctct cccagtgcc aacattcca ttacaacga atagtgcag tcagtcaaa cacaccaaa cgaaggggc tccaaggtg atcaaagag ggctggctt gtgcacttc actaacaaa gatcgcatg gtgcggcgc cacttctgg cgccttgat actaagtca attgtttta ttccaaaat gaccacact tcaaagtat tacaaggaa attccgctt tcggaaatt ctgacgatc gattcggca cgatctaaa caagctgaa gttatgcat tgtttcgaa attaggacc gcaaatgtt gattatttc gtgggtcag gaccctttg tacgacctc caagacggg aacaatgtg aacttacca ccgcctgat agcggaatt ggggcttat ttggccaaa agttgggaa acttccatc agacaagcg ctgatgcct gtgacagcc ggtccaaaa ccggaagag accaacgaa agcgaggag cacatcatg gatatgagc caaatttac cagatatat ccggacgag gttttgggt tcgggacaa ttcggaatt gtctacggg gggattcac cgaaaaacc tcaaggccc gtggctatt aaggtgatc gataagtta agatttccc accaaacaa gaagctcag ctaaaaaac gaggttgca attttacaa aacttatca caccctggt gtagttaat ctcgagcgg atgtttgaa acgcccgaa cgcattttc gtggtcatg gaaaaattg aagggcgat atgttggag atgatttta tcgcacgag aaagggcgc ttgactgaa cgcgttacg aagtttttg atcactcaa atactgata gctttaaaa caccttcat agtaaaaat atcgttcac tgtgatctg aaaccggaa aatgttctt ttgagttcg gatgcggag tttccgcaa gtgaagctg tgcgacttt gggtttgct cgaattatt ggcgagaag tcgtttcgc aggtctgtg gttggaact ccggcatat ctcgcaccg gaagttctt cgcaacaag ggctacaat aggtcgttg gatatgtgg agtgtgggt gtaatagtt tacgtgagt ctgagtgga acttttccg ttcaatgag gacgaagat attaacgaa caaattcag aatgcggct tttatgtat cctccaaat ccgtggaaa gaaatctcg tcggatgct attgatctc atcaataat ttgctgcaa gtcaagcag aggaaacgg tacactgtc gacaagtct ttgcagcat atttggctg caggactat caaacttgg tgcgatttg cgagcgttg gagaaccaa ataggcgtt cgctacttg acccacgaa tcggatgat gcacgatgg gagcgatac agggcggaa ctttga
- >TC009940 RC
atggatggt caggggcca gaagtgacg ttcctattc caatttggc ttgattcga gatgcaatc acagttcct gtggcatcg ctgaacctc aaaaccatc aaagacctg gcttgcaat tttataaac tcgaaaata cccgaccat gggatcaac aggttggag gaccgcctc ttgctgttc agacacgag tacaactcg aacaacatt cttcagttc atcaactcg gcttcggag atcgtcgac gaaaccctg atcgagatc gtcttgact ggcaaagtt tcaaccccc gaaatcaca atagtcaga ccgcatgct ctctcagtt cactcgtac aaaacacca acgttttgc gatttttgc ggcgaaatg ctgttcggg ttggtgcgc caggggctc aagtgtgaa ggatgtggg caaaattac cacaagaga tgtgtggtc aaagtcccg aacaactgc agctacact ctcttggga gacaagcag aggagacgc tcgaccact ctgcaggtt ccgcggagt ccgtcaggg ggctccaac tcgtcgcta gcgtccgcc aactcgact cagacagag gacagtggc ttgtttcag gcaccgaac aggtcacca tccctgggt gggcgaccg gcatgggtt gagaaagaa atgtcaagt cgggtaaaa attccgcac acgttcgtc ttgcacact tacacaaga ccgacagtg tgtcagtac tgtaaaaag ctgttgaaa ggactgttc aagcaaggt ctccagtgc aaagactgc aattacaac gcgcacaaa aagtgcatg gacaaagta cctaaagat tgcacgggt gaagtgcca aaggagaac tcctgcaat tacgactac gccgacagc tctgccagt aacgacagc cagtgtcac ctgaccctg gtcgaagac gagaacgaa gacgagaaa ctgaatggt gctaacaaa tatattgaa gttattccg gtgcacgat gacgacaac gactcaatt ccttccagt tgtagctct tcttcctct cccagtgcc aacattcca ttacaacga atagtgcag tcagtcaaa cacaccaaa cgaaggggc tccaaggtg atcaaagag ggctggctt gtgcacttc actaacaaa gatcgcatg gtgcggcgc cacttctgg cgccttgat actaagtca attgtttta ttccaaaat gaccacact tcaaagtat tacaaggaa attccgctt tcggaaatt ctgacgatc gattcggca cgatctaaa caagctgaa gttatgcat tgtttcgaa attaggacc gcaaatgtt gattatttc gtgggtcag gaccctttg tacgacctc caagacggg aacaatgtg aacttacca ccgcctgat agcggaatt ggggcttat ttggccaaa agttgggaa acttccatc agacaagcg ctgatgcct gtgacagcc ggtccaaaa ccggaagag accaacgaa agcgaggag cacatcatg gatatgagc caaatttac cagatatat ccggacgag gttttgggt tcgggacaa ttcggaatt gtctacggg gggattcac cgaaaaacc tcaaggccc gtggctatt aaggtgatc gataagtta agatttccc accaaacaa gaagctcag ctaaaaaac gaggttgca attttacaa aacttatca caccctggt gtagttaat ctcgagcgg atgtttgaa acgcccgaa cgcattttc gtggtcatg gaaaaattg aagggcgat atgttggag atgatttta tcgcacgag aaagggcgc ttgactgaa cgcgttacg aagtttttg atcactcaa atactgata gctttaaaa caccttcat agtaaaaat atcgttcac tgtgatctg aaaccggaa aatgttctt ttgagttcg gatgcggag tttccgcaa gtgaagctg tgcgacttt gggtttgct cgaattatt ggcgagaag tcgtttcgc aggtctgtg gttggaact ccggcatat ctcgcaccg gaagttctt cgcaacaag ggctacaat aggtcgttg gatatgtgg agtgtgggt gtaatagtt tacgtgagt ctgagtgga acttttccg ttcaatgag gacgaagat attaacgaa caaattcag aatgcggct tttatgtat cctccaaat ccgtggaaa gaaatctcg tcggatgct attgatctc atcaataat ttgctgcaa gtcaagcag aggaaacgg tacactgtc gacaagtct ttgcagcat atttggctg caggactat caaacttgg tgcgatttg cgagcgttg gagaaccaa ataggcgtt cgctacttg acccacgaa tcggatgat gcacgatgg gagcgatac agggcggaa ctttga
- >TC009940 RD
atggatggt caggggcca gaagtgacg ttcctattc caatttggc ttgattcga gatgcaatc acagttcct gtggcatcg ctgaacctc aaaaccatc aaagacctg gcttgcaat tttataaac tcgaaaata cccgaccat gggatcaac aggttggag gaccgcctc ttgctgttc agacacgag tacaactcg aacaacatt cttcagttc atcaactcg gcttcggag atcgtcgac gaaaccctg atcgagatc gtcttgact ggcaaagta gtttcaacc cccgaaatc acaatagtc agaccgcat gctctctca gttcactcg tacaaaaca ccaacgttt tgcgatttt tgcggcgaa atgctgttc gggttggtg cgccagggg ctcaagtgt gaaggatgt gggcaaaat taccacaag agatgtgtg gtcaaagtc ccgaacaac tgcagctac actctcttg ggagacaag cagaggaga cgctcgacc actctgcag gttccgcgg agtccgtca gggggctcc aactcgtcg ctagcgtcc gccaactcg actcagaca gaggacagt ggcttggta aggcttgct aattttttt accggggga gtcagtctc aaggactga
- >TC009940 RA
tcatgtttg tacgtcatt taacagtgt cttagaggt tgtttttac aaaccattt attaatgct ttatattac tgtaaatag tgaatggtt aggtgtaat tagggaacg agtgtaaat atttacgcc agtagttac gaatgtttt aggcaaaaa tggatggtc aggggccag aagtgacgt tcctattcc aatttggct tgattcgag atgcaatca cagttcctg tggcatcgc tgaacctca aaaccatca aagacctgg cttgcaatt ttataaact cgaaaatac ccgaccatg ggatcaaca ggttggagg accgcctct tgctgttca gacacgagt acaactcga acaacattc ttcagttca tcaactcgg cttcggaga tcgtcgacg aaaccctga tcgagatcg tcttgactg gcaaagttt caacccccg aaatcacaa tagtcagac cgcatgctc tctcagttc actcgtaca aaacaccaa cgttttgcg atttttgcg gcgaaatgc tgttcgggt tggtgcgcc aggggctca agtgtgaag gatgtgggc aaaattacc acaagagat gtgtggtca aagtcccga acaactgca gctacactc tcttgggag acaagcaga ggagacgct cgaccactc tgcaggttc cgcggagtc cgtcagggg gctccaact cgtcgctag cgtccgcca actcgactc agacagagg acagtggct tggtaaggc ttgctaatt tttttaccg ggggagtca gtctcaagg actgatttt ttgaccatg agctcgatc gcattccag tattttatt ctaacatga tgtacgaaa agaaaaatt tttcaaaca cagtattac taacattga aacgaaaga ttaagaatt ttctagcat ttttacaat tttgttatt ttattttat ttttgtgtt gtaagcagc gttttgttt gtattttat taacgattc cgtccatac gaagcgccg caagtggtg gaggttttc agtttgtac ttgtatttc ctttgtata ttgtttaaa tatattttg tttgactta
- >TC009940 RB
aaagtgcta agtgaagtg tagtttcag gcaccgaac aggtcacca tccctgggt gggcgaccg gcatgggtt gagaaagaa atgtcaagt cgggtaaaa attccgcac acgttcgtc ttgcacact tacacaaga ccgacagtg tgtcagtac tgtaaaaag ctgttgaaa ggactgttc aagcaaggt ctccagtgc aaagactgc aattacaac gcgcacaaa aagtgcatg gacaaagta cctaaagat tgcacgggt gaagtgcca aaggagaac tcctgcaat tacgactac gccgacagc tctgccagt aacgacagc cagtgtcac ctgaccctg gtcgaagac gagaacgaa gacgagaaa ctgaatggt gctaacaaa tatattgaa gttattccg gtgcacgat gacgacaac gactcaatt ccttccagt tgtagctct tcttcctct cccagtgcc aacattcca ttacaacga atagtgcag tcagtcaaa cacaccaaa cgaaggggc tccaaggtg atcaaagag ggctggctt gtgcacttc actaacaaa gatcgcatg gtgcggcgc cacttctgg cgccttgat actaagtca attgtttta ttccaaaat gaccacact tcaaagtat tacaaggaa attccgctt tcggaaatt ctgacgatc gattcggca cgatctaaa caagctgaa gttatgcat tgtttcgaa attaggacc gcaaatgtt gattatttc gtgggtcag gaccctttg tacgacctc caagacggg aacaatgtg aacttacca ccgcctgat agcggaatt ggggcttat ttggccaaa agttgggaa acttccatc agacaagcg ctgatgcct gtgacagcc ggtccaaaa ccggaagag accaacgaa agcgaggag cacatcatg gatatgagc caaatttac cagatatat ccggacgag gttttgggt tcgggacaa ttcggaatt gtctacggg gggattcac cgaaaaacc tcaaggccc gtggctatt aaggtgatc gataagtta agatttccc accaaacaa gaagctcag ctaaaaaac gaggttgca attttacaa aacttatca caccctggt gtagttaat ctcgagcgg atgtttgaa acgcccgaa cgcattttc gtggtcatg gaaaaattg aagggcgat atgttggag atgatttta tcgcacgag aaagggcgc ttgactgaa cgcgttacg aagtttttg atcactcaa atactgata gctttaaaa caccttcat agtaaaaat atcgttcac tgtgatctg aaaccggaa aatgttctt ttgagttcg gatgcggag tttccgcaa gtgaagctg tgcgacttt gggtttgct cgaattatt ggcgagaag tcgtttcgc aggtctgtg gttggaact ccggcatat ctcgcaccg gaagttctt cgcaacaag ggctacaat aggtcgttg gatatgtgg agtgtgggt gtaatagtt tacgtgagt ctgagtgga acttttccg ttcaatgag gacgaagat attaacgaa caaattcag aatgcggct tttatgtat cctccaaat ccgtggaaa gaaatctcg tcggatgct attgatctc atcaataat ttgctgcaa gtcaagcag aggaaacgg tacactgtc gacaagtct ttgcagcat atttggctg caggactat caaacttgg tgcgatttg cgagcgttg gagaaccaa ataggcgtt cgctacttg acccacgaa tcggatgat gcacgatgg gagcgatac agggcggaa ctttgacct gagaaaggt tggcttcac aatgtcaaa aagtgccta cgaggtatg aagacatag gcagctcaa aatctacac ggaattgct agagcacac taaacgttt ctcgataaa atttttctg tgatgtcac cttaaataa cacttcgaa tttaccaca cagatgttt tatcgtaca ctttttgaa tggtttcgc attctaaga gattctact taagtacac
- >TC009940 RC
tcatgtttg tacgtcatt taacagtgt cttagaggt tgtttttac aaaccattt attaatgct ttatattac tgtaaatag tgaatggtt aggtgtaat tagggaacg agtgtaaat atttacgcc agtagttac gaatgtttt aggcaaaaa tggatggtc aggggccag aagtgacgt tcctattcc aatttggct tgattcgag atgcaatca cagttcctg tggcatcgc tgaacctca aaaccatca aagacctgg cttgcaatt ttataaact cgaaaatac ccgaccatg ggatcaaca ggttggagg accgcctct tgctgttca gacacgagt acaactcga acaacattc ttcagttca tcaactcgg cttcggaga tcgtcgacg aaaccctga tcgagatcg tcttgactg gcaaagttt caacccccg aaatcacaa tagtcagac cgcatgctc tctcagttc actcgtaca aaacaccaa cgttttgcg atttttgcg gcgaaatgc tgttcgggt tggtgcgcc aggggctca agtgtgaag gatgtgggc aaaattacc acaagagat gtgtggtca aagtcccga acaactgca gctacactc tcttgggag acaagcaga ggagacgct cgaccactc tgcaggttc cgcggagtc cgtcagggg gctccaact cgtcgctag cgtccgcca actcgactc agacagagg acagtggct tgtttcagg caccgaaca ggtcaccat ccctgggtg ggcgaccgg catgggttg agaaagaaa tgtcaagtc gggtaaaaa ttccgcaca cgttcgtct tgcacactt acacaagac cgacagtgt gtcagtact gtaaaaagc tgttgaaag gactgttca agcaaggtc tccagtgca aagactgca attacaacg cgcacaaaa agtgcatgg acaaagtac ctaaagatt gcacgggtg aagtgccaa aggagaact cctgcaatt acgactacg ccgacagct ctgccagta acgacagcc agtgtcacc tgaccctgg tcgaagacg agaacgaag acgagaaac tgaatggtg ctaacaaat atattgaag ttattccgg tgcacgatg acgacaacg actcaattc cttccagtt gtagctctt cttcctctc ccagtgcca acattccat tacaacgaa tagtgcagt cagtcaaac acaccaaac gaaggggct ccaaggtga tcaaagagg gctggcttg tgcacttca ctaacaaag atcgcatgg tgcggcgcc acttctggc gccttgata ctaagtcaa ttgttttat tccaaaatg accacactt caaagtatt acaaggaaa ttccgcttt cggaaattc tgacgatcg attcggcac gatctaaac aagctgaag ttatgcatt gtttcgaaa ttaggaccg caaatgttg attatttcg tgggtcagg accctttgt acgacctcc aagacggga acaatgtga acttaccac cgcctgata gcggaattg gggcttatt tggccaaaa gttgggaaa cttccatca gacaagcgc tgatgcctg tgacagccg gtccaaaac cggaagaga ccaacgaaa gcgaggagc acatcatgg atatgagcc aaatttacc agatatatc cggacgagg ttttgggtt cgggacaat tcggaattg tctacgggg ggattcacc gaaaaacct caaggcccg tggctatta aggtgatcg ataagttaa gatttccca ccaaacaag aagctcagc taaaaaacg aggttgcaa ttttacaaa acttatcac accctggtg tagttaatc tcgagcgga tgtttgaaa cgcccgaac gcattttcg tggtcatgg aaaaattga agggcgata tgttggaga tgattttat cgcacgaga aagggcgct tgactgaac gcgttacga agtttttga tcactcaaa tactgatag ctttaaaac accttcata gtaaaaata tcgttcact gtgatctga aaccggaaa atgttcttt tgagttcgg atgcggagt ttccgcaag tgaagctgt gcgactttg ggtttgctc gaattattg gcgagaagt cgtttcgca ggtctgtgg ttggaactc cggcatatc tcgcaccgg aagttcttc gcaacaagg gctacaata ggtcgttgg atatgtgga gtgtgggtg taatagttt acgtgagtc tgagtggaa cttttccgt tcaatgagg acgaagata ttaacgaac aaattcaga atgcggctt ttatgtatc ctccaaatc cgtggaaag aaatctcgt cggatgcta ttgatctca tcaataatt tgctgcaag tcaagcaga ggaaacggt acactgtcg acaagtctt tgcagcata tttggctgc aggactatc aaacttggt gcgatttgc gagcgttgg agaaccaaa taggcgttc gctacttga cccacgaat cggatgatg cacgatggg agcgataca gggcggaac tttgacctg agaaaggtt ggcttcaca atgtcaaaa agtgcctac gaggtatga agacatagg cagctcaaa atctacacg gaattgcta gagcacact aaacgtttc tcgataaaa tttttctgt gatgtcacc ttaaataac acttcgaat ttaccacac agatgtttt atcgtacac tttttgaat ggtttcgca ttctaagag attctactt aagtacac
- >TC009940 RD
tcatgtttg tacgtcatt taacagtgt cttagaggt tgtttttac aaaccattt attaatgct ttatattac tgtaaatag tgaatggtt aggtgtaat tagggaacg agtgtaaat atttacgcc agtagttac gaatgtttt aggcaaaaa tggatggtc aggggccag aagtgacgt tcctattcc aatttggct tgattcgag atgcaatca cagttcctg tggcatcgc tgaacctca aaaccatca aagacctgg cttgcaatt ttataaact cgaaaatac ccgaccatg ggatcaaca ggttggagg accgcctct tgctgttca gacacgagt acaactcga acaacattc ttcagttca tcaactcgg cttcggaga tcgtcgacg aaaccctga tcgagatcg tcttgactg gcaaagtag tttcaaccc ccgaaatca caatagtca gaccgcatg ctctctcag ttcactcgt acaaaacac caacgtttt gcgattttt gcggcgaaa tgctgttcg ggttggtgc gccaggggc tcaagtgtg aaggatgtg ggcaaaatt accacaaga gatgtgtgg tcaaagtcc cgaacaact gcagctaca ctctcttgg gagacaagc agaggagac gctcgacca ctctgcagg ttccgcgga gtccgtcag ggggctcca actcgtcgc tagcgtccg ccaactcga ctcagacag aggacagtg gcttggtaa ggcttgcta attttttta ccgggggag tcagtctca aggactgat tttttgacc atgagctcg atcgcattc cagtatttt attctaaca tgatgtacg aaaagaaaa atttttcaa acacagtat tactaacat tgaaacgaa agattaaga attttctag catttttac aattttgtt attttattt tatttttgt gttgtaagc agcgttttg tttgtattt tattaacga ttccgtcca tacgaagcg ccgcaagtg gtggaggtt ttcagtttg tacttgtat ttcctttgt atattgttt aaatatatt ttgtttgac tta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
