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Gene information
General information
TC009934 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11651007..11652370
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009934
    iBeetle screen iB_04728

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009934 RA
      MSSWQNLVH KLRKNWKQF QPNLKSVRN VKTRASLTF 
      CAIGGATYW YFFYSKEPK KSITRNYSQ QTFLKFATV 
      EYKGQIYMT PSDFLSSVI ELNPKMRRN RRAKLTERQ 
      IQYIRARIP PQTKQFFSK LQNNGIISY TEYLFLLSL 
      LIKPAADIE IAFKMLDTD YNGVIDKEE FLVMETIFS 
      RSWRQKLAT RYETGLENK LDTTLAIYF FGSDGTKVL 
      SYQSFKLFV TNLQEEILQ LEFSQFAHG TFINDIDFA 
      KLLVRYTYF DNYDNYIER LLATAEREP SRDQLITFE 
      EFHSFMGFL HRLEDFYFA TRYYAEEDS QITRKGFHD 
      ILKLLGRHD LTWHMIETV FNIFDVDGT GYVNYRDLF 
      MVLNDRLNR GLTRHVEKS EWQEFKSCL RRAINPNPG 
      LAPHTRRKQ MNK
    CDS sequence
    • >TC009934 RA
      atgtcatcg tggcagaac ctcgtccac aagctgcgc 
      aaaaactgg aaacaattc caaccaaac ctaaaaagc 
      gttcgcaac gtcaaaaca cgcgcctct ctcacattt 
      tgtgcaata ggaggcgca acctattgg tattttttc 
      tattccaaa gaaccaaaa aaatcaatc acacgcaat 
      tactcccaa caaacgttt ctcaaattt gccaccgtg 
      gaatacaag ggccaaatt tacatgaca cccagcgat 
      ttcctcagt tctgtcatt gagctaaac ccaaaaatg 
      cgtcgcaac agaagagcg aagctcaca gaacgccaa 
      atacagtac atcagggca cgaattccc cctcaaacc 
      aagcaattc ttctccaaa cttcaaaat aacggaata 
      atttcctac actgagtat ttgttcctc ttatcgctc 
      ctaattaaa ccagctgct gatattgaa attgccttt 
      aaaatgctg gacacagac tacaatgga gtcatcgat 
      aaggaagag tttctggtg atggagacg atttttagc 
      cgttcttgg aggcaaaaa cttgcaact cgttacgag 
      accggcctc gaaaacaaa ctggataca actctggct 
      atatatttt tttggatca gatggaacc aaagtttta 
      agctatcaa tctttcaag ctctttgtg accaacttg 
      caggaagaa attctccaa ttggagttc agtcaattc 
      gcccatggc actttcatc aacgacata gacttcgct 
      aagcttcta gtacgatac acctatttt gacaattac 
      gacaattac attgagcgc cttttggcc acggcagaa 
      cgcgaacca tcgcgcgac caactcatc acttttgag 
      gagttccac tcattcatg gggttttta caccgactt 
      gaggatttc tacttcgca acgagatac tacgccgag 
      gaggactcc caaataact cgaaagggc ttccatgat 
      attctcaaa ttactgggc agacatgac ctaacctgg 
      cacatgata gagacagta tttaatatt tttgatgtc 
      gacggcacc ggttacgtc aactatcga gacttattt 
      atggttttg aacgatcgt ctgaatcgg ggcttaaca 
      cgccacgtc gaaaaaagc gaatggcag gaattcaaa 
      agttgccta agaagggcc attaatcca aaccctgga 
      ctagctcct cacacaaga agaaaacaa atgaacaaa 
      taa
    mRNA sequence
    • >TC009934 RA
      gcgaaaaac aatatacaa gtgacaaag gaaatcaaa 
      tgacacatt ttgcaattt ttcagtcat gtcatcgtg 
      gcagaacct cgtccacaa gctgcgcaa aaactggaa 
      acaattcca accaaacct aaaaagcgt tcgcaacgt 
      caaaacacg cgcctctct cacattttg tgcaatagg 
      aggcgcaac ctattggta ttttttcta ttccaaaga 
      accaaaaaa atcaatcac acgcaatta ctcccaaca 
      aacgtttct caaatttgc caccgtgga atacaaggg 
      ccaaattta catgacacc cagcgattt cctcagttc 
      tgtcattga gctaaaccc aaaaatgcg tcgcaacag 
      aagagcgaa gctcacaga acgccaaat acagtacat 
      cagggcacg aattccccc tcaaaccaa gcaattctt 
      ctccaaact tcaaaataa cggaataat ttcctacac 
      tgagtattt gttcctctt atcgctcct aattaaacc 
      agctgctga tattgaaat tgcctttaa aatgctgga 
      cacagacta caatggagt catcgataa ggaagagtt 
      tctggtgat ggagacgat ttttagccg ttcttggag 
      gcaaaaact tgcaactcg ttacgagac cggcctcga 
      aaacaaact ggatacaac tctggctat atatttttt 
      tggatcaga tggaaccaa agttttaag ctatcaatc 
      tttcaagct ctttgtgac caacttgca ggaagaaat 
      tctccaatt ggagttcag tcaattcgc ccatggcac 
      tttcatcaa cgacataga cttcgctaa gcttctagt 
      acgatacac ctattttga caattacga caattacat 
      tgagcgcct tttggccac ggcagaacg cgaaccatc 
      gcgcgacca actcatcac ttttgagga gttccactc 
      attcatggg gtttttaca ccgacttga ggatttcta 
      cttcgcaac gagatacta cgccgagga ggactccca 
      aataactcg aaagggctt ccatgatat tctcaaatt 
      actgggcag acatgacct aacctggca catgataga 
      gacagtatt taatatttt tgatgtcga cggcaccgg 
      ttacgtcaa ctatcgaga cttatttat ggttttgaa 
      cgatcgtct gaatcgggg cttaacacg ccacgtcga 
      aaaaagcga atggcagga attcaaaag ttgcctaag 
      aagggccat taatccaaa ccctggact agctcctca 
      cacaagaag aaaacaaat gaacaaata agtacttgt 
      tgtctgtac caacttcta atttaaaac atactgttg 
      taatgaaaa ctaaataaa aacttaatt tacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY