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Gene information
General information
TC009927 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11752297..11757771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009927
    iBeetle screen iB_04724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009927 RA
      MRSLGISVL SLTLTTIVI ANAYYQKKQ FYPSVVYIT 
      KSNPSMAVI YIQAFICVL IVGKIMRKI FFGQLRSAE 
      FEHLMERSW YAITETCLA FTVFRDDFN PKFVALFTL 
      LLFLKSFHW LAEDRVDYM ERSPVISLL FHIRVLSLL 
      LLLGSLDLH FIQHAYQST VSKGASVQL VFGFEYAIL 
      LTVVINIAI KYALHSVDL NSETPWDNK AVFLLYTEL 
      IMGFIKVIL YVAFVAIMV RIYTLPLFA FRPMYYTIR 
      NFKKAFSDV ILSRRAIYN MNTLYPDAT PEELQAADN 
      VCIICREEM TTASKKLPC NHIFHTSCL RSWFQRQQT 
      CPTCRLNIL RTPTSNNNE PRQDQRHRQ VPPPNPFFN 
      PFGGQNPFQ MFGQNNQPQ PQAQPAAAG APSGAQNAT 
      NAPQANPNI PPFVFPPFP FMPFMAPPP MPPPNLQSL 
      SPEELRRME GNERQNVEA RIQCLRNIQ VMLDAAVVM 
      MQQYSAAAA LSSQAPGGE PQPGPSTSG TPPTCNGTT 
      PKSPINLPT SSVINNNVE SDSVPSTST SSETPEATP 
      TESVALTPE EEVRRRRLQ KFQQAAEQ
    CDS sequence
    • >TC009927 RA
      atgaggtcg ttaggtata tcagttttg agcctcaca 
      ctaaccaca attgtgata gcaaatgca tactatcag 
      aaaaaacag ttctacccc tcggttgtg tatattaca 
      aaatcaaat cctagcatg gctgtcatt tatattcaa 
      gcttttata tgtgtcttg attgtgggc aaaatcatg 
      cgaaaaatc ttttttggg caactcagg tcggctgag 
      ttcgagcat ttgatggaa aggtcctgg tatgcaatc 
      acagagact tgtctagcc ttcactgtg tttagagac 
      gatttcaat ccgaagttt gtggcactg ttcacgtta 
      ctactgttt ctgaagtcg tttcattgg ctggctgaa 
      gacagggtc gattatatg gagagaagc cctgtcata 
      agtttacta ttccacata agagtacta tcattgctg 
      ttgttattg ggctccctt gacttgcat ttcatacaa 
      cacgcgtac caatcaaca gtgtcaaaa ggggcatcc 
      gttcagttg gtttttggt ttcgagtac gccatcctc 
      ctgactgtc gtcataaac atcgcaatt aaatatgcc 
      ttacattcg gttgacctc aacagcgag acgccttgg 
      gacaacaag gcagtcttt ctgttgtac actgagctc 
      atcatgggc ttcatcaaa gtcattctt tacgtggct 
      ttcgtcgct atcatggtc cgcatctat actctcccc 
      ttgttcgcg ttccgcccc atgtactac accatccgc 
      aacttcaaa aaggccttc agcgacgtg atcctctcc 
      cggcgcgcc atctacaac atgaacacc ctctacccg 
      gacgccacc cccgaggag ctgcaggcc gccgacaac 
      gtctgcatc atctgccgc gaggagatg accaccgcc 
      tccaagaaa ctaccctgc aaccacatt ttccacacg 
      tcttgtctc cgctcgtgg ttccagcgg cagcagacg 
      tgccccacc tgccgcttg aacatcctg cggacgccc 
      acgagcaac aacaacgag ccgagacag gaccagaga 
      cacagacag gtgcccccg ccgaatccg tttttcaat 
      ccgtttggt ggccagaat ccgtttcaa atgttcggc 
      cagaacaac cagccgcag ccgcaagca caaccggcg 
      gccgccggg gccccaagt ggggcgcag aatgcgacc 
      aatgcaccg caagctaat cctaatatt ccccctttt 
      gtttttcca ccgtttccg tttatgccg tttatggca 
      ccgccacca atgccgcca ccgaatctg cagtcactc 
      tcgccggag gaattgagg aggatggag ggcaatgag 
      aggcagaat gtggaggcc aggatacag tgcttgcgc 
      aatatccag gtcatgctg gacgctgct gtggtcatg 
      atgcaacaa tacagcgcg gcggcggcc ctttcgagc 
      caagccccc ggcggggag ccccagccg ggcccctcg 
      acatccggt acacccccc acctgtaat ggaacgact 
      ccaaaatcg ccaataaat ctgccaact agttcagtg 
      attaataat aatgtcgaa agtgactct gtacctagt 
      actagtacg tcgagcgag acgccagaa gcgacacca 
      actgaatca gttgctctt acgcccgaa gaagaagtg 
      aggagacgc aggttgcaa aaattccag caagctgct 
      gaacaataa
    mRNA sequence
    • >TC009927 RA
      ctcgtatat cagctgtca aaatcgtaa aaagtcaac 
      atatgaggt gaacacacg tagcaaaac tcgaacatt 
      ttcaaatta tcccatatt ttcgtctag ttgatcatg 
      ttttcagtg taataagcg tacaaggca acaatgagg 
      tcgttaggt atatcagtt ttgagcctc acactaacc 
      acaattgtg atagcaaat gcatactat cagaaaaaa 
      cagttctac ccctcggtt gtgtatatt acaaaatca 
      aatcctagc atggctgtc atttatatt caagctttt 
      atatgtgtc ttgattgtg ggcaaaatc atgcgaaaa 
      atctttttt gggcaactc aggtcggct gagttcgag 
      catttgatg gaaaggtcc tggtatgca atcacagag 
      acttgtcta gccttcact gtgtttaga gacgatttc 
      aatccgaag tttgtggca ctgttcacg ttactactg 
      tttctgaag tcgtttcat tggctggct gaagacagg 
      gtcgattat atggagaga agccctgtc ataagttta 
      ctattccac ataagagta ctatcattg ctgttgtta 
      ttgggctcc cttgacttg catttcata caacacgcg 
      taccaatca acagtgtca aaaggggca tccgttcag 
      ttggttttt ggtttcgag tacgccatc ctcctgact 
      gtcgtcata aacatcgca attaaatat gccttacat 
      tcggttgac ctcaacagc gagacgcct tgggacaac 
      aaggcagtc tttctgttg tacactgag ctcatcatg 
      ggcttcatc aaagtcatt ctttacgtg gctttcgtc 
      gctatcatg gtccgcatc tatactctc cccttgttc 
      gcgttccgc cccatgtac tacaccatc cgcaacttc 
      aaaaaggcc ttcagcgac gtgatcctc tcccggcgc 
      gccatctac aacatgaac accctctac ccggacgcc 
      acccccgag gagctgcag gccgccgac aacgtctgc 
      atcatctgc cgcgaggag atgaccacc gcctccaag 
      aaactaccc tgcaaccac attttccac acgtcttgt 
      ctccgctcg tggttccag cggcagcag acgtgcccc 
      acctgccgc ttgaacatc ctgcggacg cccacgagc 
      aacaacaac gagccgaga caggaccag agacacaga 
      caggtgccc ccgccgaat ccgtttttc aatccgttt 
      ggtggccag aatccgttt caaatgttc ggccagaac 
      aaccagccg cagccgcaa gcacaaccg gcggccgcc 
      ggggcccca agtggggcg cagaatgcg accaatgca 
      ccgcaagct aatcctaat attccccct tttgttttt 
      ccaccgttt ccgtttatg ccgtttatg gcaccgcca 
      ccaatgccg ccaccgaat ctgcagtca ctctcgccg 
      gaggaattg aggaggatg gagggcaat gagaggcag 
      aatgtggag gccaggata cagtgcttg cgcaatatc 
      caggtcatg ctggacgct gctgtggtc atgatgcaa 
      caatacagc gcggcggcg gccctttcg agccaagcc 
      cccggcggg gagccccag ccgggcccc tcgacatcc 
      ggtacaccc cccacctgt aatggaacg actccaaaa 
      tcgccaata aatctgcca actagttca gtgattaat 
      aataatgtc gaaagtgac tctgtacct agtactagt 
      acgtcgagc gagacgcca gaagcgaca ccaactgaa 
      tcagttgct cttacgccc gaagaagaa gtgaggaga 
      cgcaggttg caaaaattc cagcaagct gctgaacaa 
      taattttat tttattcca tgttgattt gggtaatca 
      attttatgt tacgataag ggccgactg tcgtaggag 
      aaattggta gtttctagt gtcaaggct tgtgctatt 
      taccacttt gtgacatca ataaatttg taaaacgcc 
      cttattgtg ttgtacata catatttaa ttattttta 
      atttctgtg taggattta tcacattta atatattcg 
      tcgatattg ttatgactt ttgagcttt atggtggtc 
      ctattatta tttgaagac tggattgtt cgtgaccaa 
      gtcactctc gacatggat gttacgtat tcactatac 
      actactgat tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY