Status
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Gene information
General information
TC009925 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11763491..11767171
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009925
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04723

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009925 RA
      MFLSDVLKK EECRNEALQ ELQKLGIKC RPKKPKQQT 
      VKATRKVFK VPLRSLSAE TVTLKTGQQ IAVPKLVHE 
      LCSFILAKV STEGLFRKE GSKARQNEI KLLLNGGGA 
      VDSNYHIID VAALLKMFL RELPEPLIP YGYHELYLR 
      CALLEKGRV RKEALLLAC LLLPLEHLN TLSYLLQFF 
      YDVTQHSVS NKMNAYNLA IVISPAIFP IEEKLSPHN 
      MQMRTAKFC DIVQILIEN APEVGILPE SIIERLGSN 
      SSVDLKKKK RRSGSLTRM FNGLKKIVG GKSEENHNV 
      VTPDLLLTP INNNVVKRR RIQSNGISN KKKKELLNK 
      LPDNVLLNT PFTPCRTPV SMDLSGTHE NHSKEPNDL 
      IKEKNWFSR SRSVKNLKP EAETKPANT KTLLERRWS 
      AITQPHNLK KAKKRNSFA ATSKKDEPD YIRVPKTEY 
      EAIKSRVSA MERRISQEL ENVQSRIEN DINEEVDVN 
      IENVQTAYE QTLVQSKLS PTADHLAKR LSRELKIRR 
      SAEQKIIRS PSARKIGSM RRKSLEKSN VKLGRHQSW 
      HVAPRESIP RVSLRRGKP NTVMTGLPT LEHKELKRT 
      PVGEPITRS VSCNLSGSS SDTWVSAEG FFETLKSND 
      SSVDGRASL ARLRSQNAG MVLAKAKLF DKLTDSDSS 
      GSVRSLPRV KTGTKIGCC RTNDSRLSY RVRSLKYEE 
      RRIGKKSMS PRKRNLTQK QKLQIAKDC LEKSSDKEN 
      IPDSFEIAK DCTNQKFAS PNYSTPRVP PHIKKSLYV 
      SSPKRLCRT PGVERSTPL RVLIN
    CDS sequence
    • >TC009925 RA
      atgtttctc agtgatgtg ctgaaaaag gaagaatgc 
      aggaacgaa gcgctccag gagctgcaa aaattgggg 
      ataaagtgt cggcccaag aaacccaaa caacagact 
      gtgaaagca acccgtaaa gtgttcaaa gttccttta 
      agatctctc tcggcggaa actgttact ttaaagacc 
      ggacagcaa attgctgtc ccgaaacta gtccatgaa 
      ttgtgttca tttatttta gccaaagtt agcacggag 
      gggctgttt aggaaagag ggctccaag gcgagacaa 
      aacgaaatt aaattgttg ctcaatggg ggcggcgct 
      gttgatagc aattaccac attatcgac gtcgcggcg 
      ctcctgaaa atgtttctt agggaatta ccggagcct 
      ctgatacct tacggctac cacgagttg tacttaaga 
      tgcgctttg ttggagaaa ggcagagtg cgaaaggaa 
      gccctgctt ttagcttgt cttttgttg ccgctggaa 
      cacctaaac actctgagt tatctgctg cagtttttt 
      tatgacgtg acgcaacac tcagtctca aataaaatg 
      aacgcttat aacctggcc atagtcata agccctgca 
      atttttccc attgaggaa aagctttcg ccacataat 
      atgcaaatg aggacggcg aaattttgt gatattgtg 
      cagattttg atagagaat gcacctgaa gtaggtatt 
      ttgccggag agcatcatc gaacgtctc ggctcgaat 
      tcaagtgtg gacttgaaa aagaagaaa cggcgcagt 
      ggatcgctc acaaggatg tttaacggc cttaaaaag 
      atagttgga gggaagtcg gaagagaat cataacgtg 
      gttacacca gacttgtta ctcactccg attaacaat 
      aatgtggtg aaaagacga cgaattcag tcgaacgga 
      atttcgaac aaaaaaaag aaggaactg ttgaataaa 
      ttacccgac aatgtttta ttaaatacg ccatttacg 
      ccgtgtcgc actccagtt agcatggac ttgagtgga 
      acacacgaa aatcactca aaagaaccg aatgactta 
      atcaaggaa aagaattgg ttttcgcgc agccgtagc 
      gtgaaaaac ctgaaaccc gaagcggaa actaaacca 
      gcaaacaca aaaacttta ctcgagcgt cgatggtcg 
      gcaataacg caaccccac aacctgaaa aaggccaag 
      aagcgcaat tccttcgcc gccacgtcg aaaaaggac 
      gagcctgac tacattcga gtcccgaaa accgaatac 
      gaagcaatc aaaagccga gtttccgca atggaacgc 
      cgaatttcg caagaactg gaaaacgtg caatcgcga 
      atcgaaaac gacatcaac gaggaagtt gacgtgaac 
      atcgaaaac gtccagact gcctacgaa cagaccttg 
      gtccagtcc aagctgagc ccgaccgcc gaccacttg 
      gccaagaga ctgagccgg gagttgaaa atccgaagg 
      agcgcagaa cagaaaata attcggtcg cccagtgcc 
      cgaaaaatc gggagcatg cggcgaaaa tcgctcgag 
      aaaagtaac gtgaaactg gggcgccac cagtcgtgg 
      cacgtggcc cccagagag agcatccct cgggtgagc 
      ttgaggcgg gggaagccc aacacggtg atgacaggt 
      ttgccaact ttggagcac aaagaactg aaacggacg 
      ccggttggg gagccaatc acacgcagc gtttcgtgc 
      aacttgtcc gggagctcc agcgacacg tgggtgagc 
      gctgagggg tttttcgag actttgaag tcgaatgac 
      tcctcggtg gacgggagg gcttccctg gcgcgtctc 
      aggagtcag aacgctggg atggtgctc gccaaagcc 
      aaacttttc gataagttg accgactcg gacagttcc 
      ggctcagtt cggagcttg ccgagggtg aagaccggg 
      acaaaaatc gggtgctgt agaaccaac gattcgcgt 
      ttgtcgtac agagtgagg agtttgaaa tacgaggag 
      aggaggatc ggaaagaag agcatgtcc ccgcgtaaa 
      cgaaattta acgcagaaa cagaagtta caaattgct 
      aaagattgt ctggagaaa agtagcgat aaggagaat 
      attcctgat agttttgaa atagcgaaa gactgcact 
      aaccaaaaa ttcgcgtca ccgaattac tcaacaccg 
      agagtgccg ccacatatc aaaaagtcg ctttatgtg 
      agctccccc aagaggctg tgtcggacg ccgggggtg 
      gagcgcagc accccgctc agagttttg attaattag
    mRNA sequence
    • >TC009925 RA
      tttgaaagt gacaactcg agcagcgac agcaatgaa 
      caacataaa ttaatttcc taataatta acgaaaccc 
      ccttctaca ttatgtttc tcagtgatg tgctgaaaa 
      aggaagaat gcaggaacg aagcgctcc aggagctgc 
      aaaaattgg ggataaagt gtcggccca agaaaccca 
      aacaacaga ctgtgaaag caacccgta aagtgttca 
      aagttcctt taagatctc tctcggcgg aaactgtta 
      ctttaaaga ccggacagc aaattgctg tcccgaaac 
      tagtccatg aattgtgtt catttattt tagccaaag 
      ttagcacgg aggggctgt ttaggaaag agggctcca 
      aggcgagac aaaacgaaa ttaaattgt tgctcaatg 
      ggggcggcg ctgttgata gcaattacc acattatcg 
      acgtcgcgg cgctcctga aaatgtttc ttagggaat 
      taccggagc ctctgatac cttacggct accacgagt 
      tgtacttaa gatgcgctt tgttggaga aaggcagag 
      tgcgaaagg aagccctgc ttttagctt gtcttttgt 
      tgccgctgg aacacctaa acactctga gttatctgc 
      tgcagtttt tttatgacg tgacgcaac actcagtct 
      caaataaaa tgaacgctt ataacctgg ccatagtca 
      taagccctg caatttttc ccattgagg aaaagcttt 
      cgccacata atatgcaaa tgaggacgg cgaaatttt 
      gtgatattg tgcagattt tgatagaga atgcacctg 
      aagtaggta ttttgccgg agagcatca tcgaacgtc 
      tcggctcga attcaagtg tggacttga aaaagaaga 
      aacggcgca gtggatcgc tcacaagga tgtttaacg 
      gccttaaaa agatagttg gagggaagt cggaagaga 
      atcataacg tggttacac cagacttgt tactcactc 
      cgattaaca ataatgtgg tgaaaagac gacgaattc 
      agtcgaacg gaatttcga acaaaaaaa agaaggaac 
      tgttgaata aattacccg acaatgttt tattaaata 
      cgccattta cgccgtgtc gcactccag ttagcatgg 
      acttgagtg gaacacacg aaaatcact caaaagaac 
      cgaatgact taatcaagg aaaagaatt ggttttcgc 
      gcagccgta gcgtgaaaa acctgaaac ccgaagcgg 
      aaactaaac cagcaaaca caaaaactt tactcgagc 
      gtcgatggt cggcaataa cgcaacccc acaacctga 
      aaaaggcca agaagcgca attccttcg ccgccacgt 
      cgaaaaagg acgagcctg actacattc gagtcccga 
      aaaccgaat acgaagcaa tcaaaagcc gagtttccg 
      caatggaac gccgaattt cgcaagaac tggaaaacg 
      tgcaatcgc gaatcgaaa acgacatca acgaggaag 
      ttgacgtga acatcgaaa acgtccaga ctgcctacg 
      aacagacct tggtccagt ccaagctga gcccgaccg 
      ccgaccact tggccaaga gactgagcc gggagttga 
      aaatccgaa ggagcgcag aacagaaaa taattcggt 
      cgcccagtg cccgaaaaa tcgggagca tgcggcgaa 
      aatcgctcg agaaaagta acgtgaaac tggggcgcc 
      accagtcgt ggcacgtgg cccccagag agagcatcc 
      ctcgggtga gcttgaggc gggggaagc ccaacacgg 
      tgatgacag gtttgccaa ctttggagc acaaagaac 
      tgaaacgga cgccggttg gggagccaa tcacacgca 
      gcgtttcgt gcaacttgt ccgggagct ccagcgaca 
      cgtgggtga gcgctgagg ggtttttcg agactttga 
      agtcgaatg actcctcgg tggacggga gggcttccc 
      tggcgcgtc tcaggagtc agaacgctg ggatggtgc 
      tcgccaaag ccaaacttt tcgataagt tgaccgact 
      cggacagtt ccggctcag ttcggagct tgccgaggg 
      tgaagaccg ggacaaaaa tcgggtgct gtagaacca 
      acgattcgc gtttgtcgt acagagtga ggagtttga 
      aatacgagg agaggagga tcggaaaga agagcatgt 
      ccccgcgta aacgaaatt taacgcaga aacagaagt 
      tacaaattg ctaaagatt gtctggaga aaagtagcg 
      ataaggaga atattcctg atagttttg aaatagcga 
      aagactgca ctaaccaaa aattcgcgt caccgaatt 
      actcaacac cgagagtgc cgccacata tcaaaaagt 
      cgctttatg tgagctccc ccaagaggc tgtgtcgga 
      cgccggggg tggagcgca gcaccccgc tcagagttt 
      tgattaatt aggagtgga aacgatttt atatatttt 
      tgccatatg ttatttgta aaatggtca cttttagga 
      ccgctttta tgattttgt gacttgtat attcgtttt 
      taaataaat aaaagtgaa agat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY