| General information | |||
| TC009918 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 11897000..11902194 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009918 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04722 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult nutritional state - Number of animals showing the phenotype: 1 (22 dpi)
- >TC009918 RA
MEGNSGDEN SDDGLQIVV DSPRPKKRA KRELDERDK KIIECVEKR LEENLEEKA AKANLTSKH VKNIIKEVV TNEHVLALV RQVERQNED DSPVYEPKF TRAKTKHLL TTNAPAIPW SKTPKKVSA VQKLFTEEL QEESSGDEY VPDTPSKNT SFDSQLETT QGTLRDEEE EANIARRTR SKLSLSHTP LEVIEEAFI PPDITTDMY DMDCDDDEW KEFLKTFTK PLDEVTKAA EDEDQDPEY NILADEEID EVDTEELRA DRAVTISRK EFNQLMTEL FEFADNYET NELNKKNAE EVANTNTDD KQDTNVESE CENDEDISI KKNQIPILV QQMTQHVQM LTQNYLMSY EHPEYAELA DKLKQMLVD LKIEGVGKT ASIYHVANL DPALNLIDD WEQLFESKS SEVKEYKAY VEKVLFKSI ASKKAGTEY IVTFPTLYL KTVSNSEVF IYPNLLPRI PFKSSQVTR RAYHYFTPG EENLIALFL EQAIPSMKQ DPEHLNKHK RINMDHVCG LINKLLMPH LPARKILRH IWTVKNTDD SNPIKFFYS HKRAPPAVH YVIPIDVNN LLPPCKQSP DSLPYLWQQ YNYPDYKHQ KSIENNITV NSSPIIVVN PPVQLWPVT TNTSASNYL HFSKKIIAK RKLEPNGSL PPVPVRKTR RRGPNKKSS ANKTVVNKE LMMLPDMPS LGGTPVKNT PEPEANPKE EGTNPSEKP PVRQSLSFE NITSFSIEF QKPDSSRLT DANAKDNPD DINELMIAS STVKTKKEP SGAEKKRAK MRREFIANI VMATPDNPE TELQKEENF AESFFDKLH QTLSVEDYE KLINILGHY DENSGQFVD LYKNVQSIL FPKYKELSE EFLLFLTEI QAKKVGQLM PYFLMKDMH RFVKKLHIY FKDQPSQLR KVIKCISDL TENPDLTLE NIKDTVLPL FKGNAALTD WFLQIFPYE RPPKLLTGR PHEVIDWSK ELARPPDSE ICETVVFPD TDDPYGGHS CVCTCHNGE NLQFKTRSQ HCNNCGLKF IQGKIFINN GKSLRPAVV SFLTNPEKD HTIRLSATP PRKRSDSSP SKQVVSPTK ENHTVEEPR QSHESEEEC DGTKKKKTK APRKRKVNP EKPAAKAKK EPKPKPVKS SPKRQNKKR APKKPPEHK PGSKAGQAK RPAEQEIEY SEPAEFVEL KVSSDHSGE SGTECFGSS QEATNTDSE DVVDSTKVS LQQNAAWSR EEDKTILQA FRERSDNEE TFKHISEVL QNRSVAEIK NRFHKLMSL LQKMASNS
- >TC009918 RA
atggagggc aactccgga gacgaaaat tctgacgac gggttgcaa attgttgtt gatagcccc cgaccgaaa aagagggct aaaagggag ctcgacgaa agagataaa aagataatt gaatgtgtt gagaaacgt ttggaggag aatctggaa gagaaggca gctaaggcg aatttaacg agcaaacat gttaaaaac ataatcaaa gaagtcgtg accaatgaa cacgtcctg gctttggtc agacaagtg gagagacaa aacgaagac gactctcca gtgtacgaa cccaaattc accagggct aagacaaag catttgtta acaacaaat gcgccagct ataccatgg agcaagacg ccgaaaaaa gtatccgca gtccaaaaa ttgttcact gaggagttg caggaagag tcatctggg gatgaatat gttcccgat actccttcg aaaaatact tcttttgat agccaactt gaaacaact caagggact ctaagagat gaagaagag gaggcaaat attgcccga aggacgagg tcaaagtta agcctcagt cacactcct ttggaagtg attgaagaa gctttcata ccgcctgat atcaccact gatatgtat gatatggac tgtgatgac gacgaatgg aaggaattt ttgaaaaca ttcactaaa cctttagat gaagtcact aaggccgct gaagacgaa gaccaagac cctgagtat aacatactc gcagatgaa gaaattgac gaagtggat acggaggaa ctgcgagct gatcgagct gtaaccatt tcgcgaaaa gaattcaac caactaatg actgaattg tttgaattc gccgataat tacgaaacc aacgaatta aataaaaaa aatgccgaa gaagtggcc aacactaac actgacgac aaacaggac accaatgta gaatcagag tgtgaaaat gacgaagac atttcgatc aagaaaaac caaattcct attttagtg caacaaatg acccagcac gtccaaatg ctgacacag aattattta atgagttat gaacatcct gagtatgcc gaattggca gataaattg aaacaaatg cttgtagac ttaaaaata gaaggagtt ggcaaaact gcctccatt taccacgta gctaattta gatcctgcg ttaaatttg attgacgat tgggaacaa ttattcgaa agtaaaagc agcgaggtg aaggaatac aaagcatat gttgaaaaa gttcttttc aaatcaata gcatcaaaa aaagctggc actgagtac attgtaacg tttccgacc ttataccta aaaactgtc tcaaacagt gaggttttt atttatcct aatttactt ccgagaatt ccatttaaa tcgtctcaa gtcacaaga cgggcgtat cattatttt actcccgga gaggaaaat ttaatagcc ttatttttg gagcaagca ataccgtct atgaaacaa gatccagaa cacttaaac aaacacaag agaattaat atggaccac gtttgtggc cttatcaat aaattattg atgcctcat ttgcccgcg cgcaaaatt ttaagacac atttggacc gtaaaaaac actgatgat tccaatcca atcaagttc ttctactcg cacaaaagg gcaccacct gcagtacat tacgtgatt cccatagat gtcaacaat ttgttaccc ccttgtaag cagagtcca gattctctt ccgtacttg tggcaacag tacaactat ccagattac aagcatcaa aaatcgatc gaaaacaac attactgta aatagtagt ccaataata gtcgtaaat ccgccggtg caactgtgg cctgtgact acaaacacg tcggcgtcc aattattta catttttcg aagaaaatt attgctaag agaaaacta gagccaaac gggtcgctt cctccggta ccggtgcga aaaacacga cgtaggggc cccaacaaa aagtctagt gctaacaaa actgtggtt aataaagaa ctgatgatg ctgcctgat atgccgagt ttggggggg accccggtc aaaaacacc ccagaacca gaggcaaac ccaaaggaa gaaggtaca aacccgagc gagaaaccc ccggtgcgc cagagtttg tcctttgaa aacattacc agtttcagt attgagttc caaaagcct gattcatcg aggcttact gatgcaaac gcaaaggac aatcccgat gatattaac gagctaatg attgctagc tctactgtt aaaacaaaa aaggaaccg tcaggtgct gagaaaaaa cgggcgaaa atgcggcga gaattcatc gctaatatc gttatggcc actccggac aatcccgaa acggagctt caaaaggaa gaaaatttt gcagaatcg ttttttgat aaactccac caaacgtta agtgtggaa gattacgag aagcttatt aatattttg gggcattat gacgaaaac agcggtcag tttgtagat ctgtacaaa aatgttcaa tcgattctg tttccaaag tacaaggag ttatctgag gaatttctc ctctttctc actgaaatt caagccaag aaggtgggc caacttatg ccttatttc ctaatgaag gacatgcat cgtttcgtg aaaaaattg cacatttat ttcaaagat cagccctct cagttgagg aaagttatc aagtgtatc agcgatttg acggaaaat cctgaccta acactggag aacatcaaa gacacggtt ttaccgcta tttaaaggc aatgcggct ttgactgat tggttttta caaattttc ccatacgag aggccccct aaactttta acagggagg ccccatgag gtcatagac tggtcaaaa gaactggca aggccgcca gattccgaa atttgcgaa actgtggta tttccagac actgatgac ccctacggg gggcatagt tgtgtctgc acttgtcac aacggtgaa aatttacaa ttcaaaacg aggtcgcaa cattgtaat aattgtgga ttaaagttt attcaaggc aaaattttt attaataat gggaaaagt ttgcggcct gcggtcgtt tcatttctc acaaatccg gaaaaagac cacacgata cgcttaagt gctacgccc ccaagaaaa cgctcagat tcgagtccg agcaaacaa gtggtgtca cctactaaa gaaaatcac accgtggag gaacctcgg cagagccac gaaagtgag gaagaatgt gacggaaca aagaaaaag aaaaccaaa gcaccgcga aagcggaaa gtaaatccc gaaaaaccg gccgctaaa gccaaaaaa gaacccaag ccaaagccg gtcaagagt tcgccaaaa cggcaaaac aaaaagcgc gctcctaaa aagccgcct gaacataaa cctgggtca aaagccggt caagcaaag cgaccagcc gaacaagaa atcgaatat tccgaaccg gcggaattt gttgaattg aaagtcagt tcagaccat agcggggaa tctggaacc gagtgcttt gggtcgtcc caagaagcc acaaatacc gacagtgag gacgtagtt gattcaaca aaagtaagt ttgcaacaa aacgccgcg tggagtcgg gaggaggac aagactatt ttacaagcg tttagagaa cggagtgat aacgaggaa acctttaaa cacatttcg gaagtgtta caaaaccga tccgttgct gaaattaag aacaggttc cacaaactg atgagtttg ttgcaaaaa atggcgtca aattcgtaa
- >TC009918 RA
ttgaggcgc tcaattttt caggttatc tcaatttca aaaagtgga tagcgttcc gagtcgttt aagaaatta aaaagatcg ttttagtgg tttgtttaa tggtgtctt catgtgcaa tatggaggg caactccgg agacgaaaa ttctgacga cgggttgca aattgttgt tgatagccc ccgaccgaa aaagagggc taaaaggga gctcgacga aagagataa aaagataat tgaatgtgt tgagaaacg tttggagga gaatctgga agagaaggc agctaaggc gaatttaac gagcaaaca tgttaaaaa cataatcaa agaagtcgt gaccaatga acacgtcct ggctttggt cagacaagt ggagagaca aaacgaaga cgactctcc agtgtacga acccaaatt caccagggc taagacaaa gcatttgtt aacaacaaa tgcgccagc tataccatg gagcaagac gccgaaaaa agtatccgc agtccaaaa attgttcac tgaggagtt gcaggaaga gtcatctgg ggatgaata tgttcccga tactccttc gaaaaatac ttcttttga tagccaact tgaaacaac tcaagggac tctaagaga tgaagaaga ggaggcaaa tattgcccg aaggacgag gtcaaagtt aagcctcag tcacactcc tttggaagt gattgaaga agctttcat accgcctga tatcaccac tgatatgta tgatatgga ctgtgatga cgacgaatg gaaggaatt tttgaaaac attcactaa acctttaga tgaagtcac taaggccgc tgaagacga agaccaaga ccctgagta taacatact cgcagatga agaaattga cgaagtgga tacggagga actgcgagc tgatcgagc tgtaaccat ttcgcgaaa agaattcaa ccaactaat gactgaatt gtttgaatt cgccgataa ttacgaaac caacgaatt aaataaaaa aaatgccga agaagtggc caacactaa cactgacga caaacagga caccaatgt agaatcaga gtgtgaaaa tgacgaaga catttcgat caagaaaaa ccaaattcc tattttagt gcaacaaat gacccagca cgtccaaat gctgacaca gaattattt aatgagtta tgaacatcc tgagtatgc cgaattggc agataaatt gaaacaaat gcttgtaga cttaaaaat agaaggagt tggcaaaac tgcctccat ttaccacgt agctaattt agatcctgc gttaaattt gattgacga ttgggaaca attattcga aagtaaaag cagcgaggt gaaggaata caaagcata tgttgaaaa agttctttt caaatcaat agcatcaaa aaaagctgg cactgagta cattgtaac gtttccgac cttatacct aaaaactgt ctcaaacag tgaggtttt tatttatcc taatttact tccgagaat tccatttaa atcgtctca agtcacaag acgggcgta tcattattt tactcccgg agaggaaaa tttaatagc cttattttt ggagcaagc aataccgtc tatgaaaca agatccaga acacttaaa caaacacaa gagaattaa tatggacca cgtttgtgg ccttatcaa taaattatt gatgcctca tttgcccgc gcgcaaaat tttaagaca catttggac cgtaaaaaa cactgatga ttccaatcc aatcaagtt cttctactc gcacaaaag ggcaccacc tgcagtaca ttacgtgat tcccataga tgtcaacaa tttgttacc cccttgtaa gcagagtcc agattctct tccgtactt gtggcaaca gtacaacta tccagatta caagcatca aaaatcgat cgaaaacaa cattactgt aaatagtag tccaataat agtcgtaaa tccgccggt gcaactgtg gcctgtgac tacaaacac gtcggcgtc caattattt acatttttc gaagaaaat tattgctaa gagaaaact agagccaaa cgggtcgct tcctccggt accggtgcg aaaaacacg acgtagggg ccccaacaa aaagtctag tgctaacaa aactgtggt taataaaga actgatgat gctgcctga tatgccgag tttgggggg gaccccggt caaaaacac cccagaacc agaggcaaa cccaaagga agaaggtac aaacccgag cgagaaacc cccggtgcg ccagagttt gtcctttga aaacattac cagtttcag tattgagtt ccaaaagcc tgattcatc gaggcttac tgatgcaaa cgcaaagga caatcccga tgatattaa cgagctaat gattgctag ctctactgt taaaacaaa aaaggaacc gtcaggtgc tgagaaaaa acgggcgaa aatgcggcg agaattcat cgctaatat cgttatggc cactccgga caatcccga aacggagct tcaaaagga agaaaattt tgcagaatc gttttttga taaactcca ccaaacgtt aagtgtgga agattacga gaagcttat taatatttt ggggcatta tgacgaaaa cagcggtca gtttgtaga tctgtacaa aaatgttca atcgattct gtttccaaa gtacaagga gttatctga ggaatttct cctctttct cactgaaat tcaagccaa gaaggtggg ccaacttat gccttattt cctaatgaa ggacatgca tcgtttcgt gaaaaaatt gcacattta tttcaaaga tcagccctc tcagttgag gaaagttat caagtgtat cagcgattt gacggaaaa tcctgacct aacactgga gaacatcaa agacacggt tttaccgct atttaaagg caatgcggc tttgactga ttggttttt acaaatttt cccatacga gaggccccc taaactttt aacagggag gccccatga ggtcataga ctggtcaaa agaactggc aaggccgcc agattccga aatttgcga aactgtggt atttccaga cactgatga cccctacgg ggggcatag ttgtgtctg cacttgtca caacggtga aaatttaca attcaaaac gaggtcgca acattgtaa taattgtgg attaaagtt tattcaagg caaaatttt tattaataa tgggaaaag tttgcggcc tgcggtcgt ttcatttct cacaaatcc ggaaaaaga ccacacgat acgcttaag tgctacgcc cccaagaaa acgctcaga ttcgagtcc gagcaaaca agtggtgtc acctactaa agaaaatca caccgtgga ggaacctcg gcagagcca cgaaagtga ggaagaatg tgacggaac aaagaaaaa gaaaaccaa agcaccgcg aaagcggaa agtaaatcc cgaaaaacc ggccgctaa agccaaaaa agaacccaa gccaaagcc ggtcaagag ttcgccaaa acggcaaaa caaaaagcg cgctcctaa aaagccgcc tgaacataa acctgggtc aaaagccgg tcaagcaaa gcgaccagc cgaacaaga aatcgaata ttccgaacc ggcggaatt tgttgaatt gaaagtcag ttcagacca tagcgggga atctggaac cgagtgctt tgggtcgtc ccaagaagc cacaaatac cgacagtga ggacgtagt tgattcaac aaaagtaag tttgcaaca aaacgccgc gtggagtcg ggaggagga caagactat tttacaagc gtttagaga acggagtga taacgagga aacctttaa acacatttc ggaagtgtt acaaaaccg atccgttgc tgaaattaa gaacaggtt ccacaaact gatgagttt gttgcaaaa aatggcgtc aaattcgta actttacat ttattaaag tgataaa
