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Gene information
General information
TC009918 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11897000..11902194
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009918
    iBeetle screen iB_04722

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009918 RA
      MEGNSGDEN SDDGLQIVV DSPRPKKRA KRELDERDK 
      KIIECVEKR LEENLEEKA AKANLTSKH VKNIIKEVV 
      TNEHVLALV RQVERQNED DSPVYEPKF TRAKTKHLL 
      TTNAPAIPW SKTPKKVSA VQKLFTEEL QEESSGDEY 
      VPDTPSKNT SFDSQLETT QGTLRDEEE EANIARRTR 
      SKLSLSHTP LEVIEEAFI PPDITTDMY DMDCDDDEW 
      KEFLKTFTK PLDEVTKAA EDEDQDPEY NILADEEID 
      EVDTEELRA DRAVTISRK EFNQLMTEL FEFADNYET 
      NELNKKNAE EVANTNTDD KQDTNVESE CENDEDISI 
      KKNQIPILV QQMTQHVQM LTQNYLMSY EHPEYAELA 
      DKLKQMLVD LKIEGVGKT ASIYHVANL DPALNLIDD 
      WEQLFESKS SEVKEYKAY VEKVLFKSI ASKKAGTEY 
      IVTFPTLYL KTVSNSEVF IYPNLLPRI PFKSSQVTR 
      RAYHYFTPG EENLIALFL EQAIPSMKQ DPEHLNKHK 
      RINMDHVCG LINKLLMPH LPARKILRH IWTVKNTDD 
      SNPIKFFYS HKRAPPAVH YVIPIDVNN LLPPCKQSP 
      DSLPYLWQQ YNYPDYKHQ KSIENNITV NSSPIIVVN 
      PPVQLWPVT TNTSASNYL HFSKKIIAK RKLEPNGSL 
      PPVPVRKTR RRGPNKKSS ANKTVVNKE LMMLPDMPS 
      LGGTPVKNT PEPEANPKE EGTNPSEKP PVRQSLSFE 
      NITSFSIEF QKPDSSRLT DANAKDNPD DINELMIAS 
      STVKTKKEP SGAEKKRAK MRREFIANI VMATPDNPE 
      TELQKEENF AESFFDKLH QTLSVEDYE KLINILGHY 
      DENSGQFVD LYKNVQSIL FPKYKELSE EFLLFLTEI 
      QAKKVGQLM PYFLMKDMH RFVKKLHIY FKDQPSQLR 
      KVIKCISDL TENPDLTLE NIKDTVLPL FKGNAALTD 
      WFLQIFPYE RPPKLLTGR PHEVIDWSK ELARPPDSE 
      ICETVVFPD TDDPYGGHS CVCTCHNGE NLQFKTRSQ 
      HCNNCGLKF IQGKIFINN GKSLRPAVV SFLTNPEKD 
      HTIRLSATP PRKRSDSSP SKQVVSPTK ENHTVEEPR 
      QSHESEEEC DGTKKKKTK APRKRKVNP EKPAAKAKK 
      EPKPKPVKS SPKRQNKKR APKKPPEHK PGSKAGQAK 
      RPAEQEIEY SEPAEFVEL KVSSDHSGE SGTECFGSS 
      QEATNTDSE DVVDSTKVS LQQNAAWSR EEDKTILQA 
      FRERSDNEE TFKHISEVL QNRSVAEIK NRFHKLMSL 
      LQKMASNS
    CDS sequence
    • >TC009918 RA
      atggagggc aactccgga gacgaaaat tctgacgac 
      gggttgcaa attgttgtt gatagcccc cgaccgaaa 
      aagagggct aaaagggag ctcgacgaa agagataaa 
      aagataatt gaatgtgtt gagaaacgt ttggaggag 
      aatctggaa gagaaggca gctaaggcg aatttaacg 
      agcaaacat gttaaaaac ataatcaaa gaagtcgtg 
      accaatgaa cacgtcctg gctttggtc agacaagtg 
      gagagacaa aacgaagac gactctcca gtgtacgaa 
      cccaaattc accagggct aagacaaag catttgtta 
      acaacaaat gcgccagct ataccatgg agcaagacg 
      ccgaaaaaa gtatccgca gtccaaaaa ttgttcact 
      gaggagttg caggaagag tcatctggg gatgaatat 
      gttcccgat actccttcg aaaaatact tcttttgat 
      agccaactt gaaacaact caagggact ctaagagat 
      gaagaagag gaggcaaat attgcccga aggacgagg 
      tcaaagtta agcctcagt cacactcct ttggaagtg 
      attgaagaa gctttcata ccgcctgat atcaccact 
      gatatgtat gatatggac tgtgatgac gacgaatgg 
      aaggaattt ttgaaaaca ttcactaaa cctttagat 
      gaagtcact aaggccgct gaagacgaa gaccaagac 
      cctgagtat aacatactc gcagatgaa gaaattgac 
      gaagtggat acggaggaa ctgcgagct gatcgagct 
      gtaaccatt tcgcgaaaa gaattcaac caactaatg 
      actgaattg tttgaattc gccgataat tacgaaacc 
      aacgaatta aataaaaaa aatgccgaa gaagtggcc 
      aacactaac actgacgac aaacaggac accaatgta 
      gaatcagag tgtgaaaat gacgaagac atttcgatc 
      aagaaaaac caaattcct attttagtg caacaaatg 
      acccagcac gtccaaatg ctgacacag aattattta 
      atgagttat gaacatcct gagtatgcc gaattggca 
      gataaattg aaacaaatg cttgtagac ttaaaaata 
      gaaggagtt ggcaaaact gcctccatt taccacgta 
      gctaattta gatcctgcg ttaaatttg attgacgat 
      tgggaacaa ttattcgaa agtaaaagc agcgaggtg 
      aaggaatac aaagcatat gttgaaaaa gttcttttc 
      aaatcaata gcatcaaaa aaagctggc actgagtac 
      attgtaacg tttccgacc ttataccta aaaactgtc 
      tcaaacagt gaggttttt atttatcct aatttactt 
      ccgagaatt ccatttaaa tcgtctcaa gtcacaaga 
      cgggcgtat cattatttt actcccgga gaggaaaat 
      ttaatagcc ttatttttg gagcaagca ataccgtct 
      atgaaacaa gatccagaa cacttaaac aaacacaag 
      agaattaat atggaccac gtttgtggc cttatcaat 
      aaattattg atgcctcat ttgcccgcg cgcaaaatt 
      ttaagacac atttggacc gtaaaaaac actgatgat 
      tccaatcca atcaagttc ttctactcg cacaaaagg 
      gcaccacct gcagtacat tacgtgatt cccatagat 
      gtcaacaat ttgttaccc ccttgtaag cagagtcca 
      gattctctt ccgtacttg tggcaacag tacaactat 
      ccagattac aagcatcaa aaatcgatc gaaaacaac 
      attactgta aatagtagt ccaataata gtcgtaaat 
      ccgccggtg caactgtgg cctgtgact acaaacacg 
      tcggcgtcc aattattta catttttcg aagaaaatt 
      attgctaag agaaaacta gagccaaac gggtcgctt 
      cctccggta ccggtgcga aaaacacga cgtaggggc 
      cccaacaaa aagtctagt gctaacaaa actgtggtt 
      aataaagaa ctgatgatg ctgcctgat atgccgagt 
      ttggggggg accccggtc aaaaacacc ccagaacca 
      gaggcaaac ccaaaggaa gaaggtaca aacccgagc 
      gagaaaccc ccggtgcgc cagagtttg tcctttgaa 
      aacattacc agtttcagt attgagttc caaaagcct 
      gattcatcg aggcttact gatgcaaac gcaaaggac 
      aatcccgat gatattaac gagctaatg attgctagc 
      tctactgtt aaaacaaaa aaggaaccg tcaggtgct 
      gagaaaaaa cgggcgaaa atgcggcga gaattcatc 
      gctaatatc gttatggcc actccggac aatcccgaa 
      acggagctt caaaaggaa gaaaatttt gcagaatcg 
      ttttttgat aaactccac caaacgtta agtgtggaa 
      gattacgag aagcttatt aatattttg gggcattat 
      gacgaaaac agcggtcag tttgtagat ctgtacaaa 
      aatgttcaa tcgattctg tttccaaag tacaaggag 
      ttatctgag gaatttctc ctctttctc actgaaatt 
      caagccaag aaggtgggc caacttatg ccttatttc 
      ctaatgaag gacatgcat cgtttcgtg aaaaaattg 
      cacatttat ttcaaagat cagccctct cagttgagg 
      aaagttatc aagtgtatc agcgatttg acggaaaat 
      cctgaccta acactggag aacatcaaa gacacggtt 
      ttaccgcta tttaaaggc aatgcggct ttgactgat 
      tggttttta caaattttc ccatacgag aggccccct 
      aaactttta acagggagg ccccatgag gtcatagac 
      tggtcaaaa gaactggca aggccgcca gattccgaa 
      atttgcgaa actgtggta tttccagac actgatgac 
      ccctacggg gggcatagt tgtgtctgc acttgtcac 
      aacggtgaa aatttacaa ttcaaaacg aggtcgcaa 
      cattgtaat aattgtgga ttaaagttt attcaaggc 
      aaaattttt attaataat gggaaaagt ttgcggcct 
      gcggtcgtt tcatttctc acaaatccg gaaaaagac 
      cacacgata cgcttaagt gctacgccc ccaagaaaa 
      cgctcagat tcgagtccg agcaaacaa gtggtgtca 
      cctactaaa gaaaatcac accgtggag gaacctcgg 
      cagagccac gaaagtgag gaagaatgt gacggaaca 
      aagaaaaag aaaaccaaa gcaccgcga aagcggaaa 
      gtaaatccc gaaaaaccg gccgctaaa gccaaaaaa 
      gaacccaag ccaaagccg gtcaagagt tcgccaaaa 
      cggcaaaac aaaaagcgc gctcctaaa aagccgcct 
      gaacataaa cctgggtca aaagccggt caagcaaag 
      cgaccagcc gaacaagaa atcgaatat tccgaaccg 
      gcggaattt gttgaattg aaagtcagt tcagaccat 
      agcggggaa tctggaacc gagtgcttt gggtcgtcc 
      caagaagcc acaaatacc gacagtgag gacgtagtt 
      gattcaaca aaagtaagt ttgcaacaa aacgccgcg 
      tggagtcgg gaggaggac aagactatt ttacaagcg 
      tttagagaa cggagtgat aacgaggaa acctttaaa 
      cacatttcg gaagtgtta caaaaccga tccgttgct 
      gaaattaag aacaggttc cacaaactg atgagtttg 
      ttgcaaaaa atggcgtca aattcgtaa
    mRNA sequence
    • >TC009918 RA
      ttgaggcgc tcaattttt caggttatc tcaatttca 
      aaaagtgga tagcgttcc gagtcgttt aagaaatta 
      aaaagatcg ttttagtgg tttgtttaa tggtgtctt 
      catgtgcaa tatggaggg caactccgg agacgaaaa 
      ttctgacga cgggttgca aattgttgt tgatagccc 
      ccgaccgaa aaagagggc taaaaggga gctcgacga 
      aagagataa aaagataat tgaatgtgt tgagaaacg 
      tttggagga gaatctgga agagaaggc agctaaggc 
      gaatttaac gagcaaaca tgttaaaaa cataatcaa 
      agaagtcgt gaccaatga acacgtcct ggctttggt 
      cagacaagt ggagagaca aaacgaaga cgactctcc 
      agtgtacga acccaaatt caccagggc taagacaaa 
      gcatttgtt aacaacaaa tgcgccagc tataccatg 
      gagcaagac gccgaaaaa agtatccgc agtccaaaa 
      attgttcac tgaggagtt gcaggaaga gtcatctgg 
      ggatgaata tgttcccga tactccttc gaaaaatac 
      ttcttttga tagccaact tgaaacaac tcaagggac 
      tctaagaga tgaagaaga ggaggcaaa tattgcccg 
      aaggacgag gtcaaagtt aagcctcag tcacactcc 
      tttggaagt gattgaaga agctttcat accgcctga 
      tatcaccac tgatatgta tgatatgga ctgtgatga 
      cgacgaatg gaaggaatt tttgaaaac attcactaa 
      acctttaga tgaagtcac taaggccgc tgaagacga 
      agaccaaga ccctgagta taacatact cgcagatga 
      agaaattga cgaagtgga tacggagga actgcgagc 
      tgatcgagc tgtaaccat ttcgcgaaa agaattcaa 
      ccaactaat gactgaatt gtttgaatt cgccgataa 
      ttacgaaac caacgaatt aaataaaaa aaatgccga 
      agaagtggc caacactaa cactgacga caaacagga 
      caccaatgt agaatcaga gtgtgaaaa tgacgaaga 
      catttcgat caagaaaaa ccaaattcc tattttagt 
      gcaacaaat gacccagca cgtccaaat gctgacaca 
      gaattattt aatgagtta tgaacatcc tgagtatgc 
      cgaattggc agataaatt gaaacaaat gcttgtaga 
      cttaaaaat agaaggagt tggcaaaac tgcctccat 
      ttaccacgt agctaattt agatcctgc gttaaattt 
      gattgacga ttgggaaca attattcga aagtaaaag 
      cagcgaggt gaaggaata caaagcata tgttgaaaa 
      agttctttt caaatcaat agcatcaaa aaaagctgg 
      cactgagta cattgtaac gtttccgac cttatacct 
      aaaaactgt ctcaaacag tgaggtttt tatttatcc 
      taatttact tccgagaat tccatttaa atcgtctca 
      agtcacaag acgggcgta tcattattt tactcccgg 
      agaggaaaa tttaatagc cttattttt ggagcaagc 
      aataccgtc tatgaaaca agatccaga acacttaaa 
      caaacacaa gagaattaa tatggacca cgtttgtgg 
      ccttatcaa taaattatt gatgcctca tttgcccgc 
      gcgcaaaat tttaagaca catttggac cgtaaaaaa 
      cactgatga ttccaatcc aatcaagtt cttctactc 
      gcacaaaag ggcaccacc tgcagtaca ttacgtgat 
      tcccataga tgtcaacaa tttgttacc cccttgtaa 
      gcagagtcc agattctct tccgtactt gtggcaaca 
      gtacaacta tccagatta caagcatca aaaatcgat 
      cgaaaacaa cattactgt aaatagtag tccaataat 
      agtcgtaaa tccgccggt gcaactgtg gcctgtgac 
      tacaaacac gtcggcgtc caattattt acatttttc 
      gaagaaaat tattgctaa gagaaaact agagccaaa 
      cgggtcgct tcctccggt accggtgcg aaaaacacg 
      acgtagggg ccccaacaa aaagtctag tgctaacaa 
      aactgtggt taataaaga actgatgat gctgcctga 
      tatgccgag tttgggggg gaccccggt caaaaacac 
      cccagaacc agaggcaaa cccaaagga agaaggtac 
      aaacccgag cgagaaacc cccggtgcg ccagagttt 
      gtcctttga aaacattac cagtttcag tattgagtt 
      ccaaaagcc tgattcatc gaggcttac tgatgcaaa 
      cgcaaagga caatcccga tgatattaa cgagctaat 
      gattgctag ctctactgt taaaacaaa aaaggaacc 
      gtcaggtgc tgagaaaaa acgggcgaa aatgcggcg 
      agaattcat cgctaatat cgttatggc cactccgga 
      caatcccga aacggagct tcaaaagga agaaaattt 
      tgcagaatc gttttttga taaactcca ccaaacgtt 
      aagtgtgga agattacga gaagcttat taatatttt 
      ggggcatta tgacgaaaa cagcggtca gtttgtaga 
      tctgtacaa aaatgttca atcgattct gtttccaaa 
      gtacaagga gttatctga ggaatttct cctctttct 
      cactgaaat tcaagccaa gaaggtggg ccaacttat 
      gccttattt cctaatgaa ggacatgca tcgtttcgt 
      gaaaaaatt gcacattta tttcaaaga tcagccctc 
      tcagttgag gaaagttat caagtgtat cagcgattt 
      gacggaaaa tcctgacct aacactgga gaacatcaa 
      agacacggt tttaccgct atttaaagg caatgcggc 
      tttgactga ttggttttt acaaatttt cccatacga 
      gaggccccc taaactttt aacagggag gccccatga 
      ggtcataga ctggtcaaa agaactggc aaggccgcc 
      agattccga aatttgcga aactgtggt atttccaga 
      cactgatga cccctacgg ggggcatag ttgtgtctg 
      cacttgtca caacggtga aaatttaca attcaaaac 
      gaggtcgca acattgtaa taattgtgg attaaagtt 
      tattcaagg caaaatttt tattaataa tgggaaaag 
      tttgcggcc tgcggtcgt ttcatttct cacaaatcc 
      ggaaaaaga ccacacgat acgcttaag tgctacgcc 
      cccaagaaa acgctcaga ttcgagtcc gagcaaaca 
      agtggtgtc acctactaa agaaaatca caccgtgga 
      ggaacctcg gcagagcca cgaaagtga ggaagaatg 
      tgacggaac aaagaaaaa gaaaaccaa agcaccgcg 
      aaagcggaa agtaaatcc cgaaaaacc ggccgctaa 
      agccaaaaa agaacccaa gccaaagcc ggtcaagag 
      ttcgccaaa acggcaaaa caaaaagcg cgctcctaa 
      aaagccgcc tgaacataa acctgggtc aaaagccgg 
      tcaagcaaa gcgaccagc cgaacaaga aatcgaata 
      ttccgaacc ggcggaatt tgttgaatt gaaagtcag 
      ttcagacca tagcgggga atctggaac cgagtgctt 
      tgggtcgtc ccaagaagc cacaaatac cgacagtga 
      ggacgtagt tgattcaac aaaagtaag tttgcaaca 
      aaacgccgc gtggagtcg ggaggagga caagactat 
      tttacaagc gtttagaga acggagtga taacgagga 
      aacctttaa acacatttc ggaagtgtt acaaaaccg 
      atccgttgc tgaaattaa gaacaggtt ccacaaact 
      gatgagttt gttgcaaaa aatggcgtc aaattcgta 
      actttacat ttattaaag tgataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY