Status
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Gene information
General information
TC033736
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009869
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12842219..12851905
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033736
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04715

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033736 RA
      MNNTTERKS PPERPPPPI VIHGKAKDH AALLQFCKK 
      QAGENFTIK YTRERTIIS TKTKKEYNS IKEKMEQDE 
      LEYHTYTTR EEKTHAFVL HGLDKGPEI PELVAEMKE 
      KGVDLINMY EMKNTQRPL FLVITGKYE TLNSISKKC 
      PALSHIRVS FHRYVKKTR ITQCHRCQE WGHATSNCR 
      VKPKCLKCA EGHWTRDCE IGEDATPKS TNKVMTVFH 
      DEIEIEDFE YDEEEEVYY YPCPCGDRF QISKEELLA 
      GEEVATCPS CSLVVKVIY DQETFQEEQ NEVKKLTED 
      LKKLEPSVE TDYLLILKQ DLIDHMQLS PSYLKLPHD 
      KNKQPEQEI DKLVAQLPS VKEKYDWTL FPSELRPKV 
      LAKRVAKKV EKNVDVQQK LTQLEQLES DDKVAKDDD 
      EEENVEDDE IVEEEQDEE MDDGTDYAN NYFDNGEDY 
      EDEDDNLDD GPIY
    CDS sequence
    • >TC033736 RA
      atgaacaac accacggag agaaaaagc cccccagaa 
      agaccccca ccgccgatt gtcattcac ggcaaagcg 
      aaagaccat gcagcactt cttcagttc tgcaagaaa 
      caagctggt gaaaatttc accatcaag tacacccgg 
      gagaggaca atcatctcc acaaaaacg aagaaagaa 
      tacaattcc atcaaggag aagatggaa caagacgaa 
      ctggagtac cacacgtac accaccagg gaggagaaa 
      acccacgcg ttcgtgctc cacggcctg gacaaaggg 
      cctgaaatt cctgaactc gtggctgag atgaaggag 
      aagggtgtt gatctcatc aacatgtat gagatgaaa 
      aacacccaa aggcccctg ttcctcgtc atcacgggg 
      aaatacgag acgctgaac agcatctcc aagaaatgc 
      ccggcacta agccacatc cgtgtgagc ttccaccgg 
      tatgtcaag aaaacgcgc atcacgcag tgccacaga 
      tgccaagaa tggggccat gcgacaagc aactgtcgc 
      gtgaaacca aaatgcctg aaatgtgcc gagggacat 
      tggacaaga gattgcgaa atcggcgaa gacgccaca 
      ccaaaatcc acaaataaa gtaatgact gtttttcac 
      gacgaaata gaaatcgag gatttcgaa tacgacgaa 
      gaggaagaa gtttactat tacccttgc ccatgcggc 
      gataggttc caaatatcc aaagaagaa ttgctcgcg 
      ggcgaagaa gtagcgact tgtccgagc tgttccttg 
      gtggttaaa gtaatttac gaccaggag acgtttcag 
      gaggagcaa aatgaagtc aaaaaacta accgaagat 
      ttgaaaaaa ttagaacca tcggtagaa acagattat 
      ctgttaatt ttaaaacag gatttaata gatcacatg 
      cagctgtca ccgtcatat ttaaaattg ccgcatgat 
      aaaaataaa caacccgaa caggaaatt gacaaattg 
      gtggcgcag ttgccaagt gtgaaagag aaatacgac 
      tggacattg ttcccttca gagctgaga ccaaaagtt 
      ttagccaaa agagtagcc aagaaagtt gagaaaaat 
      gtggacgta caacagaag ctcactcag ttggaacaa 
      ctggagagc gatgataaa gtggctaag gacgacgat 
      gaagaggag aatgtagaa gatgatgaa attgttgaa 
      gaggaacaa gacgaagaa atggatgac ggcacggat 
      tatgcaaat aattacttc gataacggc gaagattac 
      gaggatgaa gatgataac ttagatgat gggcccatt 
      tattaa
    mRNA sequence
    • >TC033736 RA
      actttatag tacagcaac atgaacaac accacggag 
      agaaaaagc cccccagaa agaccccca ccgccgatt 
      gtcattcac ggcaaagcg aaagaccat gcagcactt 
      cttcagttc tgcaagaaa caagctggt gaaaatttc 
      accatcaag tacacccgg gagaggaca atcatctcc 
      acaaaaacg aagaaagaa tacaattcc atcaaggag 
      aagatggaa caagacgaa ctggagtac cacacgtac 
      accaccagg gaggagaaa acccacgcg ttcgtgctc 
      cacggcctg gacaaaggg cctgaaatt cctgaactc 
      gtggctgag atgaaggag aagggtgtt gatctcatc 
      aacatgtat gagatgaaa aacacccaa aggcccctg 
      ttcctcgtc atcacgggg aaatacgag acgctgaac 
      agcatctcc aagaaatgc ccggcacta agccacatc 
      cgtgtgagc ttccaccgg tatgtcaag aaaacgcgc 
      atcacgcag tgccacaga tgccaagaa tggggccat 
      gcgacaagc aactgtcgc gtgaaacca aaatgcctg 
      aaatgtgcc gagggacat tggacaaga gattgcgaa 
      atcggcgaa gacgccaca ccaaaatcc acaaataaa 
      gtaatgact gtttttcac gacgaaata gaaatcgag 
      gatttcgaa tacgacgaa gaggaagaa gtttactat 
      tacccttgc ccatgcggc gataggttc caaatatcc 
      aaagaagaa ttgctcgcg ggcgaagaa gtagcgact 
      tgtccgagc tgttccttg gtggttaaa gtaatttac 
      gaccaggag acgtttcag gaggagcaa aatgaagtc 
      aaaaaacta accgaagat ttgaaaaaa ttagaacca 
      tcggtagaa acagattat ctgttaatt ttaaaacag 
      gatttaata gatcacatg cagctgtca ccgtcatat 
      ttaaaattg ccgcatgat aaaaataaa caacccgaa 
      caggaaatt gacaaattg gtggcgcag ttgccaagt 
      gtgaaagag aaatacgac tggacattg ttcccttca 
      gagctgaga ccaaaagtt ttagccaaa agagtagcc 
      aagaaagtt gagaaaaat gtggacgta caacagaag 
      ctcactcag ttggaacaa ctggagagc gatgataaa 
      gtggctaag gacgacgat gaagaggag aatgtagaa 
      gatgatgaa attgttgaa gaggaacaa gacgaagaa 
      atggatgac ggcacggat tatgcaaat aattacttc 
      gataacggc gaagattac gaggatgaa gatgataac 
      ttagatgat gggcccatt tattaattt atttatttt 
      tacaaaatg tatatagcg taataaatt gaaagtttt 
      tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY