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Gene information
General information
TC009857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9220744..9222392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009857
      iBeetle screen iB_04714

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva segment anterior decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      head partially randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      prothorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009857 RA
        MAEQDDNWF GSWLNAAKD KSSKVLEFV KKDLEEFGT 
        AVKNEASYV VSSTGNVIE KTFKLDTPD STASSMKRS 
        FSSFLGQMN TVLNPTPDD SDTEAILIG EDSETVPLT 
        KLQKAIYEL QKEDVTFTN DPEEALAKK FECWLEIID 
        DQLSEDRIN KHLTSSTVL RNQYEKLVP DTVPHSLFW 
        KRYLFKRAL LEDEIAHQE AMEKKEKKE KAITVETVK 
        WDQEDLAPY IELTEEEQI KLLQEYENE IKNKQDTKC 
        KESKKEAPM SAIPDTCDT NSKVSNLEE EMKTSETKA 
        INKVDSAIS LETTSSNSS TDGDWEKIG DIDK
      CDS sequence
      • >TC009857 RA
        atggcagaa caggatgat aactggttt ggtagctgg 
        ctgaacgcc gcgaaagat aagagctct aaagtgctg 
        gaatttgtc aaaaaagat ttggaagag ttcgggacc 
        gctgttaaa aatgaggcg agttatgtg gtttcctct 
        acaggaaat gttattgag aaaactttt aagctggac 
        actccagac tcaactgcc agctccatg aaaaggagt 
        tttagttct tttcttggt caaatgaac actgtccta 
        aaccccacc cctgacgat tcagacact gaagccatc 
        ctaattggt gaagattca gaaaccgtt cccttgact 
        aagttacaa aaagccatt tacgaatta caaaaagag 
        gatgtcact ttcacaaac gatcctgaa gaagcccta 
        gcgaagaaa tttgagtgc tggctggag atcattgac 
        gatcagctg tctgaagac aggataaat aaacactta 
        accagttcc actgtgctt agaaaccaa tacgagaaa 
        cttgtgcct gacactgtg ccacactct ttattttgg 
        aaaaggtat ttatttaaa agagcgctg ttagaagat 
        gagatcgct caccaggaa gccatggag aagaaggag 
        aaaaaggaa aaagccatc acggttgag acggtcaag 
        tgggatcaa gaagatctc gcaccatac attgagctg 
        acagaagaa gagcaaatc aagctactt caagagtac 
        gaaaatgag attaagaac aaacaggac accaaatgt 
        aaggaaagt aagaaagaa gcaccgatg tcggcaatt 
        ccagacact tgtgatact aattctaaa gtctcaaac 
        ttagaagaa gaaatgaaa acctccgaa acgaaagcc 
        attaataaa gtcgactcc gcgattagt ttagaaacg 
        actagcagt aatagcagt acagacggt gattgggaa 
        aagatcggc gatattgat aaatag
      mRNA sequence
      • >TC009857 RA
        gctctaagg catcacttg tgttaaaaa agaaaacaa 
        aacgtcaaa attactgtt tgtcctgat aaaaactca 
        aaatttcac gtgaaattt tcgttggtt cgttagaaa 
        aaccaagta atggcagaa caggatgat aactggttt 
        ggtagctgg ctgaacgcc gcgaaagat aagagctct 
        aaagtgctg gaatttgtc aaaaaagat ttggaagag 
        ttcgggacc gctgttaaa aatgaggcg agttatgtg 
        gtttcctct acaggaaat gttattgag aaaactttt 
        aagctggac actccagac tcaactgcc agctccatg 
        aaaaggagt tttagttct tttcttggt caaatgaac 
        actgtccta aaccccacc cctgacgat tcagacact 
        gaagccatc ctaattggt gaagattca gaaaccgtt 
        cccttgact aagttacaa aaagccatt tacgaatta 
        caaaaagag gatgtcact ttcacaaac gatcctgaa 
        gaagcccta gcgaagaaa tttgagtgc tggctggag 
        atcattgac gatcagctg tctgaagac aggataaat 
        aaacactta accagttcc actgtgctt agaaaccaa 
        tacgagaaa cttgtgcct gacactgtg ccacactct 
        ttattttgg aaaaggtat ttatttaaa agagcgctg 
        ttagaagat gagatcgct caccaggaa gccatggag 
        aagaaggag aaaaaggaa aaagccatc acggttgag 
        acggtcaag tgggatcaa gaagatctc gcaccatac 
        attgagctg acagaagaa gagcaaatc aagctactt 
        caagagtac gaaaatgag attaagaac aaacaggac 
        accaaatgt aaggaaagt aagaaagaa gcaccgatg 
        tcggcaatt ccagacact tgtgatact aattctaaa 
        gtctcaaac ttagaagaa gaaatgaaa acctccgaa 
        acgaaagcc attaataaa gtcgactcc gcgattagt 
        ttagaaacg actagcagt aatagcagt acagacggt 
        gattgggaa aagatcggc gatattgat aaataggtc 
        aatgtattc aggatttat atttaatac tttttatca 
        tacttgtag ttcacaatg caattttgg gtttttatt 
        tatgaattt tgcaacaat aaaatgttt ttactagt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY