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Gene information
General information
TC009847 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9040431..9042057
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009847
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04713

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009847 RA
      MASQLLHVT RTLSKLTLN TKCRVTNTL IKLERELPK 
      QPLFNHASR NTNFFNKMP AEDLWKGIT TVSNAGKKR 
      GRGKTVSKK NIKDLNRGQ IIGVGKANM LWPGLTAPV 
      IRGKEIVEQ QQLPEDPER QARLNQLRD QMGTHRHAK 
      LSPIERGWS GAKMPGRSI GPPDPVGED HFDGFDTKV 
      LELKTVFNM KGNFGRKRR VSATVITGN KNGLVGFAN 
      GKGVEPRTA MRKAKNRSA QKLMHIKIF RNHTVYHDF 
      FTQFAATKI YVWQKPEGY GLECHRAIK TICEVVGIK 
      DLHAKVEGS TNVTYVMKA FLLGLVRQK THQEIAEEK 
      KLHLVEFTK ETGCFPTVL ASPAQCRKE DQLGKDEIL 
      DFTQYCFDG KVILKKKKY PPFYTKLKS YETYLKKQE 
      KLRNKDKVK LDLIAQYGE IRSFLTEKY PECRPFRRA 
      KKEEEQEAE A
    CDS sequence
    • >TC009847 RA
      atggcctct caactcctt catgtgaca agaacacta 
      tcaaaattg actttaaat acaaaatgt cgagttact 
      aacacttta attaagtta gaacgcgaa ttacccaag 
      cagcccctc ttcaatcat gcttctcga aacacgaat 
      ttttttaac aaaatgcca gctgaagac ctttggaaa 
      ggaatcacg actgtaagt aatgccggt aaaaaacga 
      ggtcgtggt aaaaccgtc tcaaagaaa aacattaag 
      gatttgaat aggggccaa atcattgga gtaggaaag 
      gccaatatg ttgtggcca ggcctcaca gcccccgta 
      atccgggga aaggaaata gtagagcaa caacaactc 
      cctgaagac ccagaacgc caagctaga ctcaaccag 
      ttgcgggac caaatgggg acacaccgt cacgccaaa 
      ctgagcccc attgaaagg ggctggtct ggggcgaaa 
      atgccggga cggagcatt ggaccccca gatccagtt 
      ggggaagac cattttgac ggtttcgat actaaagtc 
      ctagaattg aaaaccgtg ttcaacatg aagggaaac 
      ttcggccgc aaacgccgc gtgtctgca accgttatc 
      accggtaac aagaacggt ttggttggc ttcgccaac 
      ggcaagggt gtcgaacct cgaacagca atgcgcaaa 
      gctaaaaac cgctcagca cagaaacta atgcacata 
      aaaatattc cgaaaccac accgtctac cacgacttt 
      ttcacccaa tttgcggcc actaaaatt tacgtctgg 
      cagaaaccc gaaggctac ggtttggag tgccacagg 
      gctatcaaa acaatctgc gaagttgtg gggatcaaa 
      gatctgcac gcgaaagtt gaggggtcc acaaacgtg 
      acttatgta atgaaagcg ttccttttg ggtctagtt 
      agacaaaaa acgcaccag gaaatcgcc gaggaaaag 
      aaactccat ctggtggag ttcaccaag gagactggg 
      tgttttccc actgtgcta gcttcgccg gcgcagtgt 
      agaaaagag gatcagctc gggaaagac gaaatttta 
      gattttacg caatattgt tttgatggg aaagttatc 
      ctaaagaag aagaaatat cccccgttc tataccaag 
      ctgaagtct tacgagact tatttgaag aagcaggaa 
      aagttgagg aataaggat aaggtcaag ctcgatttg 
      attgcgcaa tatggcgaa attaggagc tttctgacg 
      gagaagtac cccgagtgc aggccgttc aggcgtgca 
      aagaaggaa gaagagcag gaagcggaa gcgtag
    mRNA sequence
    • >TC009847 RA
      attgtcaac atggccgga aaaaggtta tgaaataca 
      agcactaaa aaattgtta ataacttta aaaaatggc 
      ctctcaact ccttcatgt gacaagaac actatcaaa 
      attgacttt aaatacaaa atgtcgagt tactaacac 
      tttaattaa gttagaacg cgaattacc caagcagcc 
      cctcttcaa tcatgcttc tcgaaacac gaatttttt 
      taacaaaat gccagctga agacctttg gaaaggaat 
      cacgactgt aagtaatgc cggtaaaaa acgaggtcg 
      tggtaaaac cgtctcaaa gaaaaacat taaggattt 
      gaatagggg ccaaatcat tggagtagg aaaggccaa 
      tatgttgtg gccaggcct cacagcccc cgtaatccg 
      gggaaagga aatagtaga gcaacaaca actccctga 
      agacccaga acgccaagc tagactcaa ccagttgcg 
      ggaccaaat ggggacaca ccgtcacgc caaactgag 
      ccccattga aaggggctg gtctggggc gaaaatgcc 
      gggacggag cattggacc cccagatcc agttgggga 
      agaccattt tgacggttt cgatactaa agtcctaga 
      attgaaaac cgtgttcaa catgaaggg aaacttcgg 
      ccgcaaacg ccgcgtgtc tgcaaccgt tatcaccgg 
      taacaagaa cggtttggt tggcttcgc caacggcaa 
      gggtgtcga acctcgaac agcaatgcg caaagctaa 
      aaaccgctc agcacagaa actaatgca cataaaaat 
      attccgaaa ccacaccgt ctaccacga ctttttcac 
      ccaatttgc ggccactaa aatttacgt ctggcagaa 
      acccgaagg ctacggttt ggagtgcca cagggctat 
      caaaacaat ctgcgaagt tgtggggat caaagatct 
      gcacgcgaa agttgaggg gtccacaaa cgtgactta 
      tgtaatgaa agcgttcct tttgggtct agttagaca 
      aaaaacgca ccaggaaat cgccgagga aaagaaact 
      ccatctggt ggagttcac caaggagac tgggtgttt 
      tcccactgt gctagcttc gccggcgca gtgtagaaa 
      agaggatca gctcgggaa agacgaaat tttagattt 
      tacgcaata ttgttttga tgggaaagt tatcctaaa 
      gaagaagaa atatccccc gttctatac caagctgaa 
      gtcttacga gacttattt gaagaagca ggaaaagtt 
      gaggaataa ggataaggt caagctcga tttgattgc 
      gcaatatgg cgaaattag gagctttct gacggagaa 
      gtaccccga gtgcaggcc gttcaggcg tgcaaagaa 
      ggaagaaga gcaggaagc ggaagcgta gtgtaaata 
      aactagcga agttacaaa tatttgtta atactactt 
      gaataataa aaaatacaa ggaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY