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Gene information
General information
TC009842 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8946267..8949064
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009842
    iBeetle screen iB_04712

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009842 RA
      MSGKPLLYS YWRSSCSWR VRIALNLKE 
      IPYDIKPVS LIKTGGEQH TNEFREVNP 
      MEQVPALHI DGVTLVESL SILAYLEET 
      RPQRPLLPH DVVKRAKVR EICEVIASG 
      IQPLQNLVV LIHVGEEKK NEWAQHWIN 
      RGFRAVEKL LSASAGKYC VGDEITLAD 
      CCLIPQVFN ARRFHVDLR PFPIILRID 
      RELENHPAF RAAHPSNQP DCPPEIAK
    CDS sequence
    • >TC009842 RA
      atgtctggt aagcctctt ttgtattca tattggcgc 
      agttcgtgc tcgtggagg gtccggata gccctaaat 
      ttaaaagaa atcccctac gacatcaaa cccgtgtct 
      ctcatcaaa accgggggt gagcagcac acaaacgag 
      ttcagagag gtcaacccg atggaacaa gtgcctgcc 
      ctccacatc gacggagtc accttggtg gagtctttg 
      agtatatta gcttatctg gaagaaacg aggccccaa 
      aggccgctg cttccgcac gacgtggtc aaacgtgca 
      aaggtccgc gaaatttgc gaagtaatt gcgtcagga 
      atccagcct ttgcaaaac ctcgtcgtc ctcattcac 
      gtcggggag gaaaaaaag aacgaatgg gctcaacac 
      tggatcaac cggggcttc agagccgtg gagaagctg 
      ctctcggcc agcgcggga aagtactgc gtcggggac 
      gaaatcact ctagcggat tgttgttta ataccacaa 
      gttttcaac gcgaggaga tttcacgtg gacctgcga 
      ccgtttcca attattttg cggattgat agggaattg 
      gagaatcat ccggcgttc cgagcggct catccgagc 
      aatcagcca gactgtccc cccgaaatt gccaagtaa
    mRNA sequence
    • >TC009842 RA
      catctgtaa agcgctgaa tcatgttgt ttatccaac 
      tttttagat atatcttat taaaattcg ttattaaaa 
      tgcgtgcga aaaccagtt aaatagcga agggtttgc 
      gctttgaat agaacaagt ttgcaaata aaccagtcg 
      cacacaatc aactcattg tagggttgc tgtaaaagc 
      tgtcgctga tttcagttt ttgagcacg cttcgtgcg 
      atcgattca tttgtatgt atctctgag cgaaattaa 
      agtaacgaa aatgtctgg taagcctct tttgtattc 
      atattggcg cagttcgtg ctcgtggag ggtccggat 
      agccctaaa tttaaaaga aatccccta cgacatcaa 
      acccgtgtc tctcatcaa aaccggggg tgagcagca 
      cacaaacga gttcagaga ggtcaaccc gatggaaca 
      agtgcctgc cctccacat cgacggagt caccttggt 
      ggagtcttt gagtatatt agcttatct ggaagaaac 
      gaggcccca aaggccgct gcttccgca cgacgtggt 
      caaacgtgc aaaggtccg cgaaatttg cgaagtaat 
      tgcgtcagg aatccagcc tttgcaaaa cctcgtcgt 
      cctcattca cgtcgggga ggaaaaaaa gaacgaatg 
      ggctcaaca ctggatcaa ccggggctt cagagccgt 
      ggagaagct gctctcggc cagcgcggg aaagtactg 
      cgtcgggga cgaaatcac tctagcgga ttgttgttt 
      aataccaca agttttcaa cgcgaggag atttcacgt 
      ggacctgcg accgtttcc aattatttt gcggattga 
      tagggaatt ggagaatca tccggcgtt ccgagcggc 
      tcatccgag caatcagcc agactgtcc ccccgaaat 
      tgccaagta atcggaaaa cagacggtt tttttattg 
      ctcaatcaa agtacaaat tgtttcagc cctgaccct 
      tacgtttga tttattggt tttggtagg tttatgcat 
      tcgttgaaa ttctagatc tgatatttt ttaatgctt 
      tttaataaa tgtgttttt tgcaaaata agtgatggt 
      ttaaatcag ccttgtctt tatttagtt tcgtgtacg 
      tacaatatt gacattgtt caataacaa caaattaca 
      aatttggaa ttcgactag attattagt ttaaaatgg 
      acggaccta aagtatcat gaatttcga aacgaaaaa 
      attgaatga atcaagcgc tataataca agcaaataa 
      ggaacaaaa taaagattg gatgaggca agaactact 
      gcggttttt gtatgatgc aagctctgt aagtgtaca 
      aagaccggg gagcactct tttggaatt ttcgctaaa 
      atccatcac attcagaat gattgtccc atcgagaag 
      gcttcacaa tagagtgct ccaaatgga catgttaat 
      gttgcgtgt catgaggaa tcatgaata atacgatat 
      atggcacta tggatttta ttgaattat agtgtgttt 
      gacaatgct gtggactaa cttattaaa agaaaaaca 
      aaaacatgt acagacact gtgcttagg tccacgcag 
      acggaacct aagaacact gccacagca actttgaaa 
      gaactataa acccgtgtc acctcctta aaactgtaa 
      actattatc actgtacaa acaattctt gactaactg 
      taacaaact gttacgtat caaccataa cagaacctt 
      caagtgcga aaatcggct tttgttaaa acagtgcaa 
      gaaaacaca cttccccta cacaaaact ggtaactta 
      attgttaaa tgtcggact tggatgttg ccaatcggc 
      cacatgcac acacacaat cataaggta cctttagcc 
      ccgatttac ttactccta atctatcat taccacgaa 
      atgactaaa tctcttaca ctaacttag aataaatca 
      tacatggct acaagaggg cgctgtgtg ggcccggca 
      ccccgatat aggcctctt tttttaaaa agggcacca 
      acggaccca cacgacacc ctttataaa aatatacaa 
      aagaaagcg aatctggaa aaacccgag tcgggcaaa 
      ctcaagccg tttgctcgc ccccgatcc gaaaaacaa 
      ctgaattcc gacgatagt gtcactctg gcattaaaa 
      acaagtgaa tacgagtca caacatgtt ttcttattt 
      aattacgtc tgatgaagg tcactttaa tttgcactg 
      caagtagga gagaacggg aaaaatggc gtggtttta 
      atgccacag tgacacgag attggccaa tggcaagca 
      cgggatgtt tctcgtgac tcgactatc tattataat 
      tatttttct tcaatatat tataatatg aactctagc 
      taaattcac agttacccc cgagagcgg tggattgcc 
      cgtcatcta cgataacag acaaaccac gtgactatg 
      atgacaatg taatattgc acacacttg gtgaaatga 
      cgggcaacc aaagcctga gacaggtaa ccccacaga 
      tgtgaatat cacagcaac agtaatgtt gtatataga 
      acagttttc ttcattaaa tatatcaaa gtatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY