Status
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Gene information
General information
TC009841 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8939377..8944721
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009841
    iBeetle screen iB_04711

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009841 RA
      MKWIILILL YPTIQCQED ENLEIVSKT RTSYLQKYG 
      GYVDVVVMH VEVPPEVSF ASLKFTADE TKASIFGCK 
      PRKVLLYMK HKSLPVINP DGSKFPDSF KNISRSQYL 
      YLELMSDKS VKYLNLTSP DPGCYFITA FLPYNNPKH 
      ESIQPPELT PECYAFVET TLYIRRRPS AGQIVEGAS 
      YNVETFTSH SILYTFFVP EFVTHATFL VDNITFAKT 
      ATRLVIRIQ SRSIPSENN TIRTEIIEP SYTNKVTIP 
      FNTEEDNWH YIEFLFEGP ASPTESKSS LKFTLKYFA 
      EDSERTSNN SVWRIFNSD QITKVTKYK QYDLVRESS 
      TESFSYSYE LRQKFTISS NVAVNLTNK ELAVLQFKL 
      REHADIGGT LQFILAFKP RINKSKRII EDEPADNKI 
      IACIRPEAP ELPTWPNLC NYDDNNIVA PLVLGKSTD 
      NSSMMIPYP ESGVWYATF KLFCGECVP CSCPNDCQK 
      KFKACVKEC QANCVKKET CNNCSLDCK TEVVNSNGC 
      AKCDCDGPC LRSKKECNS SVLFDISST ACANNCGKH 
      GRCMFMVCD GVVYATCVC TDNYRGWDC SDASEATPY 
      YMIVIELLL LVLSNLMFL PTVYVAYRR KYYVEAIVY 
      FAICFFSTF YHACDAGEN VISFCITRL GALQFADFY 
      CALLAIWVT LIAIADLPS YWPTMCHIT GAIILSFTT 
      TINKTGIWG FLLPVISGL VIIAINWFL KWKRVKRLF 
      LHKRYLYVF IPLGMLVVC VGLTIFIFL ETSNNYKYL 
      HSLWHVLMA VGVMILLPQ PNTFQPETV L
    CDS sequence
    • >TC009841 RA
      atgaaatgg ataattcta atccttctg tacccgaca 
      atacaatgc caagaggac gaaaatttg gaaatcgtg 
      tccaagact cgaacgtcc tacctgcag aaatatggg 
      ggctacgtg gacgtggtc gtgatgcat gtggaagtc 
      cctccagag gtgtctttc gcatcactg aagttcacg 
      gcagatgaa accaaggcc tcgatattt ggttgcaaa 
      ccacggaaa gtattattg tacatgaag cacaagtct 
      ttgccagtg atcaatcca gacgggtcg aagttccca 
      gacagtttt aagaacatt tcgaggtca cagtatttg 
      taccttgag ttgatgagc gacaaaagt gtgaaatat 
      ttgaatttg acgtcgccc gatccaggg tgctatttt 
      ataactgcc tttttgcca tacaataac cccaagcac 
      gagtccatc caaccacca gagttgaca ccggagtgc 
      tacgctttt gtggaaacc accctgtac ataaggcgg 
      aggcctagc gctggccag atcgtcgag ggcgcctct 
      tacaacgtc gagaccttc acctcgcac agtattctc 
      tatacattt ttcgtcccc gaattcgtc acacacgcc 
      accttcctt gtggacaac atcaccttc gcaaagact 
      gcaactcgt cttgtaatt cgtatccaa tcccgttcc 
      atcccttcc gagaacaat acaatcaga actgaaata 
      atagagccc tcttatacc aacaaagtc actattccc 
      ttcaacacg gaagaggat aactggcat tacatcgaa 
      ttcctcttc gaaggacct gcttcaccc accgagagt 
      aaatctagt cttaaattc accctcaag tacttcgct 
      gaagactcg gagaggacg tccaataat tcagtttgg 
      cgaatattc aacagcgac caaataaca aaagtgacc 
      aagtacaag cagtacgat ttagtaagg gagtccagc 
      accgaaagc ttctcctat tcgtacgaa ctgaggcaa 
      aaattcacg atttcgtca aatgttgcc gtgaatttg 
      accaataaa gaattagca gtgctccag ttcaaactg 
      cgggaacat gcggatatc ggcggcacg ctccagttt 
      atcctagcg ttcaaacca cgcatcaac aaaagcaaa 
      cggataatc gaggacgag cccgcagat aacaaaatc 
      atagcttgt attcggccc gaggctcca gaattgcca 
      acttggccc aacttgtgt aactacgac gataacaac 
      attgtggcc cctctggtg ttgggcaaa tctaccgat 
      aactcctct atgatgatc ccctatccc gaatcgggg 
      gtctggtac gcaaccttc aagttgttc tgtggcgaa 
      tgtgtgcct tgcagttgt ccgaatgat tgccagaag 
      aagttcaaa gcgtgcgtc aaggagtgc caagcgaat 
      tgtgtgaag aaagagact tgtaacaat tgttcactt 
      gactgcaag actgaagtt gtcaattcc aacggttgt 
      gcgaaatgt gactgtgat gggccgtgt cttaggagc 
      aaaaaagaa tgcaattcg agtgtcctt ttcgatatt 
      tcgtcaacg gcgtgtgcc aataactgc ggaaaacac 
      ggaaggtgc atgttcatg gtctgcgat ggcgtcgtc 
      tatgccacc tgtgtctgc actgacaat tacagaggc 
      tgggattgc tcggacgcc tctgaggcc accccatac 
      tacatgatc gtcatcgaa cttcttcta ttggtcctc 
      agcaacttg atgtttctg ccgacggtg tatgtggca 
      taccgtcgc aaatactac gtcgaagct atagtttat 
      ttcgcaatt tgcttcttc tcaaccttc taccacgcg 
      tgtgatgct ggcgaaaac gtgattagt ttttgtata 
      acacgcttg ggggcgttg cagttcgcc gatttctat 
      tgtgccctt ctggccatt tgggtcacg ctgatcgca 
      attgctgac ttgccctct tattggcca actatgtgt 
      catataacc ggcgctatt atcttgtct ttcacgacg 
      actataaat aaaactgga atttggggg tttcttttg 
      cccgttatt tcgggtctt gtgattata gcgattaat 
      tggttcttg aagtggaag agagttaag cggttgttt 
      ttgcacaag aggtatttg tacgttttt ataccgctg 
      ggtatgctc gtggtttgt gtcgggttg acgattttt 
      atatttttg gaaacgtct aataattat aagtatttg 
      cacagtctg tggcacgtt ttgatggct gttggtgtt 
      atgatactg ttgccgcaa ccgaatacg tttcagccg 
      gaaacggtg ttgtaa
    mRNA sequence
    • >TC009841 RA
      atcggcata aacaaacaa tacattatt gccccgacc 
      aatgaaagc ccacagtcg ggcccaaaa tttaaaaat 
      aaatccatt catttaaaa cgatcaatt atcttgtgc 
      gttggttga taagagaag catcgagtg ggatgatct 
      cataaagcg acttcctgc tgataaagc cctgttttc 
      ctcgccttc cagcaaatc tataatgaa atggataat 
      tctaatcct tctgtaccc gacaataca atgccaaga 
      ggacgaaaa tttggaaat cgtgtccaa gactcgaac 
      gtcctacct gcagaaata tgggggcta cgtggacgt 
      ggtcgtgat gcatgtgga agtccctcc agaggtgtc 
      tttcgcatc actgaagtt cacggcaga tgaaaccaa 
      ggcctcgat atttggttg caaaccacg gaaagtatt 
      attgtacat gaagcacaa gtctttgcc agtgatcaa 
      tccagacgg gtcgaagtt cccagacag ttttaagaa 
      catttcgag gtcacagta tttgtacct tgagttgat 
      gagcgacaa aagtgtgaa atatttgaa tttgacgtc 
      gcccgatcc agggtgcta ttttataac tgccttttt 
      gccatacaa taaccccaa gcacgagtc catccaacc 
      accagagtt gacaccgga gtgctacgc ttttgtgga 
      aaccaccct gtacataag gcggaggcc tagcgctgg 
      ccagatcgt cgagggcgc ctcttacaa cgtcgagac 
      cttcacctc gcacagtat tctctatac atttttcgt 
      ccccgaatt cgtcacaca cgccacctt ccttgtgga 
      caacatcac cttcgcaaa gactgcaac tcgtcttgt 
      aattcgtat ccaatcccg ttccatccc ttccgagaa 
      caatacaat cagaactga aataataga gccctctta 
      taccaacaa agtcactat tcccttcaa cacggaaga 
      ggataactg gcattacat cgaattcct cttcgaagg 
      acctgcttc acccaccga gagtaaatc tagtcttaa 
      attcaccct caagtactt cgctgaaga ctcggagag 
      gacgtccaa taattcagt ttggcgaat attcaacag 
      cgaccaaat aacaaaagt gaccaagta caagcagta 
      cgatttagt aagggagtc cagcaccga aagcttctc 
      ctattcgta cgaactgag gcaaaaatt cacgatttc 
      gtcaaatgt tgccgtgaa tttgaccaa taaagaatt 
      agcagtgct ccagttcaa actgcggga acatgcgga 
      tatcggcgg cacgctcca gtttatcct agcgttcaa 
      accacgcat caacaaaag caaacggat aatcgagga 
      cgagcccgc agataacaa aatcatagc ttgtattcg 
      gcccgaggc tccagaatt gccaacttg gcccaactt 
      gtgtaacta cgacgataa caacattgt ggcccctct 
      ggtgttggg caaatctac cgataactc ctctatgat 
      gatccccta tcccgaatc gggggtctg gtacgcaac 
      cttcaagtt gttctgtgg cgaatgtgt gccttgcag 
      ttgtccgaa tgattgcca gaagaagtt caaagcgtg 
      cgtcaagga gtgccaagc gaattgtgt gaagaaaga 
      gacttgtaa caattgttc acttgactg caagactga 
      agttgtcaa ttccaacgg ttgtgcgaa atgtgactg 
      tgatgggcc gtgtcttag gagcaaaaa agaatgcaa 
      ttcgagtgt ccttttcga tatttcgtc aacggcgtg 
      tgccaataa ctgcggaaa acacggaag gtgcatgtt 
      catggtctg cgatggcgt cgtctatgc cacctgtgt 
      ctgcactga caattacag aggctggga ttgctcgga 
      cgcctctga ggccacccc atactacat gatcgtcat 
      cgaacttct tctattggt cctcagcaa cttgatgtt 
      tctgccgac ggtgtatgt ggcataccg tcgcaaata 
      ctacgtcga agctatagt ttatttcgc aatttgctt 
      cttctcaac cttctacca cgcgtgtga tgctggcga 
      aaacgtgat tagtttttg tataacacg cttgggggc 
      gttgcagtt cgccgattt ctattgtgc ccttctggc 
      catttgggt cacgctgat cgcaattgc tgacttgcc 
      ctcttattg gccaactat gtgtcatat aaccggcgc 
      tattatctt gtctttcac gacgactat aaataaaac 
      tggaatttg ggggtttct tttgcccgt tatttcggg 
      tcttgtgat tatagcgat taattggtt cttgaagtg 
      gaagagagt taagcggtt gtttttgca caagaggta 
      tttgtacgt ttttatacc gctgggtat gctcgtggt 
      ttgtgtcgg gttgacgat ttttatatt tttggaaac 
      gtctaataa ttataagta tttgcacag tctgtggca 
      cgttttgat ggctgttgg tgttatgat actgttgcc 
      gcaaccgaa tacgtttca gccggaaac ggtgttgta 
      actttcgtc caatttctc tttaagatt agaaatgcg 
      tttatgtgt ttatttaga cgtccattg tttcaggga 
      ccgtctcga acaaagatt tataaccgt aagtcaaag 
      aattttgtg ttccctcct tagctagcg acttcttgt 
      acccaaagt tgccttcta ctataatgt aattaagaa 
      aagcactga gtgtgagaa tagtccttc aatattttt 
      ctcattatg aaaattgtg attttcgat tggaaatgt 
      aaaacagta gattacctt tatgacact atttgttag 
      gatactttt tacaatgct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY