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Gene information
General information
TC009839 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8928497..8929829
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009839
    iBeetle screen iB_04710

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009839 RA
      MQQREEKQL ESSLDSIIQ RVNDLKSSI ASMIYKVEN 
      EYETLNWPT FLDNYALMS GQLTSLSKV LSHDKCPTL 
      RNLTVLPLM LSPERDEQL AQMTEHRVT TFAHDLVPD 
      YLRTKLEPL AENKMMQLE HKASTLSYE NAQKQIAAY 
      QKVVSHVWE LVSKAREEW EVESSARGS TQQNSSVAD 
      THSLVAAVG MGKGLKMGA NPNGQPNPT SGGMMVPPQ 
      GRPGGTPGQ GQMPPSTQT MTMNKAPSA IKTNIKSAT 
      QIHPYGR
    CDS sequence
    • >TC009839 RA
      atgcaacaa cgggaagaa aaacaactc gaatcgtct 
      ctcgattcc ataatccag cgtgtcaac gacctaaaa 
      tcatcgata gcttcaatg atttacaag gtggagaat 
      gaatatgag accctcaac tggcccaca tttttggac 
      aattacgcc cttatgtcc ggccaactc acctccctt 
      tcgaaggtt ttaagccac gacaaatgc cccaccctc 
      agaaatctc acagtactg cccctcatg ttgtccccc 
      gagcgggac gaacaattg gcccaaatg accgaacac 
      agagtcaca accttcgcc catgattta gtcccagat 
      tatcttcga accaaacta gaaccgttg gcggagaat 
      aaaatgatg caattggaa cacaaggcg tcaacttta 
      tcgtatgag aacgcacag aaacaaatt gctgcgtat 
      caaaaggtt gtgtcccac gtttgggaa ctggtgagt 
      aaagcgagg gaggagtgg gaagttgag tcttccgcc 
      aggggaagt acgcagcag aattccagt gtggctgat 
      actcactcg cttgtggcg gctgttgga atggggaag 
      ggactcaag atgggggcg aaccctaac ggtcagccc 
      aatcccacg tcgggaggg atgatggtg cctccgcag 
      gggcgccca gggggcact cccgggcaa ggacagatg 
      ccccccagc acgcaaacc atgacgatg aataaggcg 
      ccctctgct attaagact aacatcaaa agtgccacc 
      caaatacat ccatatgga cgctag
    mRNA sequence
    • >TC009839 RA
      cggcaatgc cacagccga cataccgtc atcgtggtt 
      aaggttatg tttttgtaa acagcgcca aaaatacag 
      caaaatgca acaacggga agaaaaaca actcgaatc 
      gtctctcga ttccataat ccagcgtgt caacgacct 
      aaaatcatc gatagcttc aatgattta caaggtgga 
      gaatgaata tgagaccct caactggcc cacattttt 
      ggacaatta cgcccttat gtccggcca actcacctc 
      cctttcgaa ggttttaag ccacgacaa atgccccac 
      cctcagaaa tctcacagt actgcccct catgttgtc 
      ccccgagcg ggacgaaca attggccca aatgaccga 
      acacagagt cacaacctt cgcccatga tttagtccc 
      agattatct tcgaaccaa actagaacc gttggcgga 
      gaataaaat gatgcaatt ggaacacaa ggcgtcaac 
      tttatcgta tgagaacgc acagaaaca aattgctgc 
      gtatcaaaa ggttgtgtc ccacgtttg ggaactggt 
      gagtaaagc gagggagga gtgggaagt tgagtcttc 
      cgccagggg aagtacgca gcagaattc cagtgtggc 
      tgatactca ctcgcttgt ggcggctgt tggaatggg 
      gaagggact caagatggg ggcgaaccc taacggtca 
      gcccaatcc cacgtcggg agggatgat ggtgcctcc 
      gcaggggcg cccaggggg cactcccgg gcaaggaca 
      gatgccccc cagcacgca aaccatgac gatgaataa 
      ggcgccctc tgctattaa gactaacat caaaagtgc 
      cacccaaat acatccata tggacgcta gtgaaatag 
      tttttaatg tgtacggag atatattta tttattaag 
      atataatct tacaaaaat aactaaata taacagtgg 
      aaacaaaga ttaacactt tgtagcgta atgagtaga 
      ttataaaaa gcattaatt ttatagact ttgaaacta 
      tactatcaa caaatggtt gaagtgtgg ctggtcgga 
      acgggtggc ttttccaat aagtacagc ggaacttga 
      acattttca cgatgtatg ttagcacat gctacgcat 
      tatataaaa tataaaaca tataacatt tagaatact 
      tgggaccca gtcaaacaa aaattaaca acttaaaaa 
      aatattttc tacaacgta acgcgggtg actggaccc 
      tgtttacgt ataatacgc atttaaaaa aatataaca 
      aacaacaaa taaaatact cttgtgcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY