Status
Please wait ...
Gene information
General information
TC009819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8612856..8616132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009819
    iBeetle screen iB_04708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009819 RA
      MSSLLERVR GHSRASSGS REFLEATKR DALNLLEKE 
LDKLLSTAP DNEKEKIRK EFDGFAALF GRFLEETGP 
SVDWDKIEK LPNDAVRDY STLETPTAD TIHNMLNKL 
VVVKLNGGL GTSMGCHGP KSVIAVRNE LTFLDLTVQ 
QIEHLNKTY KVNVPLILM NSFNTDEDT QKVIRKYKN 
LQVEIHTFN QSCYPRINR DTLMPITKK IDIHNDLES 
WYPPGHGDF YQSFKNSGL LRKFIQEGR EYCFISNID 
NLGATVDLN ILNMVLNPK DKVQHEFVM EVTDKTRAD 
VKGGTLIQY ENKLRLLEI AQVPKEHVD DFKSVKTFK 
FFNTNNLWA KLDAIERVL AQGSLSLEI IINNKSLNN 
GLNVIQLET AVGAAMKSF EAGIGINVP RSRFLPVKK 
TQDLLLVMS NLYSLKNGS LVMSPQRMF PTTPLVKLG 
DNHFAKVKD FLGRFATIP DLIELDHLT VSGDVTFGR 
GVSLKGTVI IIANHGERI DIPSGANLE NKIVSGNMR 
ILDH
    CDS sequence
    • >TC009819 RA
      atgagctct ctgctagaa cgggtccgg ggccattcg 
agggcctcc agcggctcg cgcgaattt ctggaagcc 
accaaacgg gacgccttg aacctcctg gaaaaggaa 
ctggacaag cttctatca acggcgccc gacaatgaa 
aaggagaag atccggaaa gagttcgac ggatttgca 
gcactcttt ggaaggttt ctcgaagaa actggacct 
tctgttgat tgggataaa attgagaag ttgcctaat 
gatgctgtt agagattac agtactctg gagacgcca 
actgcagat actatacac aatatgctc aataaattg 
gtagttgtt aagctgaac ggagggctc gggacgtcc 
atgggctgc cacgggccc aaatcggtg atcgcggtc 
agaaacgaa ctcactttc ctcgactta accgtgcag 
caaatcgaa cacttgaac aagacttac aaagttaac 
gttcctcta atactcatg aattcgttc aacacggat 
gaagacacg caaaaagtg atccgcaaa tacaagaat 
cttcaagtt gaaattcac acttttaac cagtcgtgt 
tatcccaga atcaatagg gatactcta atgcctatc 
acgaagaaa atcgatatt cataacgac ctggaatcg 
tggtatcct ccaggtcac ggcgatttt taccaaagt 
ttcaaaaat tcaggcctc ttgcgcaaa ttcatacaa 
gaagggcgg gaatattgc tttatttct aacatcgat 
aatctggga gctacagtt gacttgaat attcttaac 
atggtactg aatccgaaa gataaagtc caacatgaa 
ttcgttatg gaagtcacc gataagact agggctgac 
gtcaagggc ggtactttg atccaatac gagaacaaa 
ctgcgctta ttggaaatc gcccaagtg cccaaagag 
cacgtcgac gacttcaag tcggtcaaa acgttcaaa 
tttttcaat acgaataac ttgtgggca aaacttgac 
gctattgag cgagtctta gctcagggt tcgcttagt 
cttgagatt attatcaat aataaaagt ctgaacaat 
ggtttgaat gtgattcag ttggagacg gctgttggg 
gcggcgatg aaatcgttt gaggcaggc attggcata 
aatgtgccg cggagtaga tttttgccg gtgaaaaaa 
acacaggac ttgcttcta gtcatgagt aatttgtac 
agtttgaaa aatgggtcg ttggtgatg tcgccacaa 
aggatgttc cccactacg ccactggtt aagttaggg 
gataatcat ttcgcgaaa gtgaaggac tttttgggg 
cgctttgcg acgattccc gatttgatc gagttggac 
catttgacc gtttcgggg gatgttacg tttggcagg 
ggcgtctcg ttgaagggt actgtaatt ataatcgcg 
aatcacgga gaacggatc gatattccg tcaggtgcc 
aatttagaa aataagata gtgtccggt aatatgcgg 
attctggat cattga
    mRNA sequence
    • >TC009819 RA
      accgagtca tgtgcgtgc tgtaaatga ccacttaca 
taattagca atatcactt ttgtttatc catgccgtt 
cttctcaaa cgaacgata agggcccga atgtctaat 
aatagtcga ctctctcaa aacttatta tcgcggaaa 
cgaaactta tcaactcgg atataaact aagtgtcca 
tcaggagcg atcagtaat cgttgcttt tccacacac 
tcgtaatga gctctctgc tagaacggg tccggggcc 
attcgaggg cctccagcg gctcgcgcg aatttctgg 
aagccacca aacgggacg ccttgaacc tcctggaaa 
aggaactgg acaagcttc tatcaacgg cgcccgaca 
atgaaaagg agaagatcc ggaaagagt tcgacggat 
ttgcagcac tctttggaa ggtttctcg aagaaactg 
gaccttctg ttgattggg ataaaattg agaagttgc 
ctaatgatg ctgttagag attacagta ctctggaga 
cgccaactg cagatacta tacacaata tgctcaata 
aattggtag ttgttaagc tgaacggag ggctcggga 
cgtccatgg gctgccacg ggcccaaat cggtgatcg 
cggtcagaa acgaactca ctttcctcg acttaaccg 
tgcagcaaa tcgaacact tgaacaaga cttacaaag 
ttaacgttc ctctaatac tcatgaatt cgttcaaca 
cggatgaag acacgcaaa aagtgatcc gcaaataca 
agaatcttc aagttgaaa ttcacactt ttaaccagt 
cgtgttatc ccagaatca atagggata ctctaatgc 
ctatcacga agaaaatcg atattcata acgacctgg 
aatcgtggt atcctccag gtcacggcg atttttacc 
aaagtttca aaaattcag gcctcttgc gcaaattca 
tacaagaag ggcgggaat attgcttta tttctaaca 
tcgataatc tgggagcta cagttgact tgaatattc 
ttaacatgg tactgaatc cgaaagata aagtccaac 
atgaattcg ttatggaag tcaccgata agactaggg 
ctgacgtca agggcggta ctttgatcc aatacgaga 
acaaactgc gcttattgg aaatcgccc aagtgccca 
aagagcacg tcgacgact tcaagtcgg tcaaaacgt 
tcaaatttt tcaatacga ataacttgt gggcaaaac 
ttgacgcta ttgagcgag tcttagctc agggttcgc 
ttagtcttg agattatta tcaataata aaagtctga 
acaatggtt tgaatgtga ttcagttgg agacggctg 
ttggggcgg cgatgaaat cgtttgagg caggcattg 
gcataaatg tgccgcgga gtagatttt tgccggtga 
aaaaaacac aggacttgc ttctagtca tgagtaatt 
tgtacagtt tgaaaaatg ggtcgttgg tgatgtcgc 
cacaaagga tgttcccca ctacgccac tggttaagt 
taggggata atcatttcg cgaaagtga aggactttt 
tggggcgct ttgcgacga ttcccgatt tgatcgagt 
tggaccatt tgaccgttt cgggggatg ttacgtttg 
gcaggggcg tctcgttga agggtactg taattataa 
tcgcgaatc acggagaac ggatcgata ttccgtcag 
gtgccaatt tagaaaata agatagtgt ccggtaata 
tgcggattc tggatcatt gagtaacga gtatgcctg 
tgtagattt tgttgtttt atttgtata tgttgttat 
agtttttct acacgtaaa tttacagta actgtagtc 
attgtttgc tatttattt atgaagatt tattgccta 
gttttacaa taaatatat tttcggcat atttttggg 
attttgctt ttaacgtag gcaaccaac aatacaaac 
atacccttt ctaacaata ccaccttgt cctaccacc 
actttttat ggcggcgtt ttgattaat taattaatg 
atgcaataa taaattgaa aaccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY