| General information | |||
| TC009819 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8612856..8616132 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009819 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04708 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
- >TC009819 RA
MSSLLERVR GHSRASSGS REFLEATKR DALNLLEKE LDKLLSTAP DNEKEKIRK EFDGFAALF GRFLEETGP SVDWDKIEK LPNDAVRDY STLETPTAD TIHNMLNKL VVVKLNGGL GTSMGCHGP KSVIAVRNE LTFLDLTVQ QIEHLNKTY KVNVPLILM NSFNTDEDT QKVIRKYKN LQVEIHTFN QSCYPRINR DTLMPITKK IDIHNDLES WYPPGHGDF YQSFKNSGL LRKFIQEGR EYCFISNID NLGATVDLN ILNMVLNPK DKVQHEFVM EVTDKTRAD VKGGTLIQY ENKLRLLEI AQVPKEHVD DFKSVKTFK FFNTNNLWA KLDAIERVL AQGSLSLEI IINNKSLNN GLNVIQLET AVGAAMKSF EAGIGINVP RSRFLPVKK TQDLLLVMS NLYSLKNGS LVMSPQRMF PTTPLVKLG DNHFAKVKD FLGRFATIP DLIELDHLT VSGDVTFGR GVSLKGTVI IIANHGERI DIPSGANLE NKIVSGNMR ILDH
- >TC009819 RA
atgagctct ctgctagaa cgggtccgg ggccattcg agggcctcc agcggctcg cgcgaattt ctggaagcc accaaacgg gacgccttg aacctcctg gaaaaggaa ctggacaag cttctatca acggcgccc gacaatgaa aaggagaag atccggaaa gagttcgac ggatttgca gcactcttt ggaaggttt ctcgaagaa actggacct tctgttgat tgggataaa attgagaag ttgcctaat gatgctgtt agagattac agtactctg gagacgcca actgcagat actatacac aatatgctc aataaattg gtagttgtt aagctgaac ggagggctc gggacgtcc atgggctgc cacgggccc aaatcggtg atcgcggtc agaaacgaa ctcactttc ctcgactta accgtgcag caaatcgaa cacttgaac aagacttac aaagttaac gttcctcta atactcatg aattcgttc aacacggat gaagacacg caaaaagtg atccgcaaa tacaagaat cttcaagtt gaaattcac acttttaac cagtcgtgt tatcccaga atcaatagg gatactcta atgcctatc acgaagaaa atcgatatt cataacgac ctggaatcg tggtatcct ccaggtcac ggcgatttt taccaaagt ttcaaaaat tcaggcctc ttgcgcaaa ttcatacaa gaagggcgg gaatattgc tttatttct aacatcgat aatctggga gctacagtt gacttgaat attcttaac atggtactg aatccgaaa gataaagtc caacatgaa ttcgttatg gaagtcacc gataagact agggctgac gtcaagggc ggtactttg atccaatac gagaacaaa ctgcgctta ttggaaatc gcccaagtg cccaaagag cacgtcgac gacttcaag tcggtcaaa acgttcaaa tttttcaat acgaataac ttgtgggca aaacttgac gctattgag cgagtctta gctcagggt tcgcttagt cttgagatt attatcaat aataaaagt ctgaacaat ggtttgaat gtgattcag ttggagacg gctgttggg gcggcgatg aaatcgttt gaggcaggc attggcata aatgtgccg cggagtaga tttttgccg gtgaaaaaa acacaggac ttgcttcta gtcatgagt aatttgtac agtttgaaa aatgggtcg ttggtgatg tcgccacaa aggatgttc cccactacg ccactggtt aagttaggg gataatcat ttcgcgaaa gtgaaggac tttttgggg cgctttgcg acgattccc gatttgatc gagttggac catttgacc gtttcgggg gatgttacg tttggcagg ggcgtctcg ttgaagggt actgtaatt ataatcgcg aatcacgga gaacggatc gatattccg tcaggtgcc aatttagaa aataagata gtgtccggt aatatgcgg attctggat cattga
- >TC009819 RA
accgagtca tgtgcgtgc tgtaaatga ccacttaca taattagca atatcactt ttgtttatc catgccgtt cttctcaaa cgaacgata agggcccga atgtctaat aatagtcga ctctctcaa aacttatta tcgcggaaa cgaaactta tcaactcgg atataaact aagtgtcca tcaggagcg atcagtaat cgttgcttt tccacacac tcgtaatga gctctctgc tagaacggg tccggggcc attcgaggg cctccagcg gctcgcgcg aatttctgg aagccacca aacgggacg ccttgaacc tcctggaaa aggaactgg acaagcttc tatcaacgg cgcccgaca atgaaaagg agaagatcc ggaaagagt tcgacggat ttgcagcac tctttggaa ggtttctcg aagaaactg gaccttctg ttgattggg ataaaattg agaagttgc ctaatgatg ctgttagag attacagta ctctggaga cgccaactg cagatacta tacacaata tgctcaata aattggtag ttgttaagc tgaacggag ggctcggga cgtccatgg gctgccacg ggcccaaat cggtgatcg cggtcagaa acgaactca ctttcctcg acttaaccg tgcagcaaa tcgaacact tgaacaaga cttacaaag ttaacgttc ctctaatac tcatgaatt cgttcaaca cggatgaag acacgcaaa aagtgatcc gcaaataca agaatcttc aagttgaaa ttcacactt ttaaccagt cgtgttatc ccagaatca atagggata ctctaatgc ctatcacga agaaaatcg atattcata acgacctgg aatcgtggt atcctccag gtcacggcg atttttacc aaagtttca aaaattcag gcctcttgc gcaaattca tacaagaag ggcgggaat attgcttta tttctaaca tcgataatc tgggagcta cagttgact tgaatattc ttaacatgg tactgaatc cgaaagata aagtccaac atgaattcg ttatggaag tcaccgata agactaggg ctgacgtca agggcggta ctttgatcc aatacgaga acaaactgc gcttattgg aaatcgccc aagtgccca aagagcacg tcgacgact tcaagtcgg tcaaaacgt tcaaatttt tcaatacga ataacttgt gggcaaaac ttgacgcta ttgagcgag tcttagctc agggttcgc ttagtcttg agattatta tcaataata aaagtctga acaatggtt tgaatgtga ttcagttgg agacggctg ttggggcgg cgatgaaat cgtttgagg caggcattg gcataaatg tgccgcgga gtagatttt tgccggtga aaaaaacac aggacttgc ttctagtca tgagtaatt tgtacagtt tgaaaaatg ggtcgttgg tgatgtcgc cacaaagga tgttcccca ctacgccac tggttaagt taggggata atcatttcg cgaaagtga aggactttt tggggcgct ttgcgacga ttcccgatt tgatcgagt tggaccatt tgaccgttt cgggggatg ttacgtttg gcaggggcg tctcgttga agggtactg taattataa tcgcgaatc acggagaac ggatcgata ttccgtcag gtgccaatt tagaaaata agatagtgt ccggtaata tgcggattc tggatcatt gagtaacga gtatgcctg tgtagattt tgttgtttt atttgtata tgttgttat agtttttct acacgtaaa tttacagta actgtagtc attgtttgc tatttattt atgaagatt tattgccta gttttacaa taaatatat tttcggcat atttttggg attttgctt ttaacgtag gcaaccaac aatacaaac atacccttt ctaacaata ccaccttgt cctaccacc actttttat ggcggcgtt ttgattaat taattaatg atgcaataa taaattgaa aaccat
