Status
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Gene information
General information
TC009819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8612856..8616132
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009819
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009819 RA
      MSSLLERVR GHSRASSGS REFLEATKR DALNLLEKE 
      LDKLLSTAP DNEKEKIRK EFDGFAALF GRFLEETGP 
      SVDWDKIEK LPNDAVRDY STLETPTAD TIHNMLNKL 
      VVVKLNGGL GTSMGCHGP KSVIAVRNE LTFLDLTVQ 
      QIEHLNKTY KVNVPLILM NSFNTDEDT QKVIRKYKN 
      LQVEIHTFN QSCYPRINR DTLMPITKK IDIHNDLES 
      WYPPGHGDF YQSFKNSGL LRKFIQEGR EYCFISNID 
      NLGATVDLN ILNMVLNPK DKVQHEFVM EVTDKTRAD 
      VKGGTLIQY ENKLRLLEI AQVPKEHVD DFKSVKTFK 
      FFNTNNLWA KLDAIERVL AQGSLSLEI IINNKSLNN 
      GLNVIQLET AVGAAMKSF EAGIGINVP RSRFLPVKK 
      TQDLLLVMS NLYSLKNGS LVMSPQRMF PTTPLVKLG 
      DNHFAKVKD FLGRFATIP DLIELDHLT VSGDVTFGR 
      GVSLKGTVI IIANHGERI DIPSGANLE NKIVSGNMR 
      ILDH
    CDS sequence
    • >TC009819 RA
      atgagctct ctgctagaa cgggtccgg ggccattcg 
      agggcctcc agcggctcg cgcgaattt ctggaagcc 
      accaaacgg gacgccttg aacctcctg gaaaaggaa 
      ctggacaag cttctatca acggcgccc gacaatgaa 
      aaggagaag atccggaaa gagttcgac ggatttgca 
      gcactcttt ggaaggttt ctcgaagaa actggacct 
      tctgttgat tgggataaa attgagaag ttgcctaat 
      gatgctgtt agagattac agtactctg gagacgcca 
      actgcagat actatacac aatatgctc aataaattg 
      gtagttgtt aagctgaac ggagggctc gggacgtcc 
      atgggctgc cacgggccc aaatcggtg atcgcggtc 
      agaaacgaa ctcactttc ctcgactta accgtgcag 
      caaatcgaa cacttgaac aagacttac aaagttaac 
      gttcctcta atactcatg aattcgttc aacacggat 
      gaagacacg caaaaagtg atccgcaaa tacaagaat 
      cttcaagtt gaaattcac acttttaac cagtcgtgt 
      tatcccaga atcaatagg gatactcta atgcctatc 
      acgaagaaa atcgatatt cataacgac ctggaatcg 
      tggtatcct ccaggtcac ggcgatttt taccaaagt 
      ttcaaaaat tcaggcctc ttgcgcaaa ttcatacaa 
      gaagggcgg gaatattgc tttatttct aacatcgat 
      aatctggga gctacagtt gacttgaat attcttaac 
      atggtactg aatccgaaa gataaagtc caacatgaa 
      ttcgttatg gaagtcacc gataagact agggctgac 
      gtcaagggc ggtactttg atccaatac gagaacaaa 
      ctgcgctta ttggaaatc gcccaagtg cccaaagag 
      cacgtcgac gacttcaag tcggtcaaa acgttcaaa 
      tttttcaat acgaataac ttgtgggca aaacttgac 
      gctattgag cgagtctta gctcagggt tcgcttagt 
      cttgagatt attatcaat aataaaagt ctgaacaat 
      ggtttgaat gtgattcag ttggagacg gctgttggg 
      gcggcgatg aaatcgttt gaggcaggc attggcata 
      aatgtgccg cggagtaga tttttgccg gtgaaaaaa 
      acacaggac ttgcttcta gtcatgagt aatttgtac 
      agtttgaaa aatgggtcg ttggtgatg tcgccacaa 
      aggatgttc cccactacg ccactggtt aagttaggg 
      gataatcat ttcgcgaaa gtgaaggac tttttgggg 
      cgctttgcg acgattccc gatttgatc gagttggac 
      catttgacc gtttcgggg gatgttacg tttggcagg 
      ggcgtctcg ttgaagggt actgtaatt ataatcgcg 
      aatcacgga gaacggatc gatattccg tcaggtgcc 
      aatttagaa aataagata gtgtccggt aatatgcgg 
      attctggat cattga
    mRNA sequence
    • >TC009819 RA
      accgagtca tgtgcgtgc tgtaaatga ccacttaca 
      taattagca atatcactt ttgtttatc catgccgtt 
      cttctcaaa cgaacgata agggcccga atgtctaat 
      aatagtcga ctctctcaa aacttatta tcgcggaaa 
      cgaaactta tcaactcgg atataaact aagtgtcca 
      tcaggagcg atcagtaat cgttgcttt tccacacac 
      tcgtaatga gctctctgc tagaacggg tccggggcc 
      attcgaggg cctccagcg gctcgcgcg aatttctgg 
      aagccacca aacgggacg ccttgaacc tcctggaaa 
      aggaactgg acaagcttc tatcaacgg cgcccgaca 
      atgaaaagg agaagatcc ggaaagagt tcgacggat 
      ttgcagcac tctttggaa ggtttctcg aagaaactg 
      gaccttctg ttgattggg ataaaattg agaagttgc 
      ctaatgatg ctgttagag attacagta ctctggaga 
      cgccaactg cagatacta tacacaata tgctcaata 
      aattggtag ttgttaagc tgaacggag ggctcggga 
      cgtccatgg gctgccacg ggcccaaat cggtgatcg 
      cggtcagaa acgaactca ctttcctcg acttaaccg 
      tgcagcaaa tcgaacact tgaacaaga cttacaaag 
      ttaacgttc ctctaatac tcatgaatt cgttcaaca 
      cggatgaag acacgcaaa aagtgatcc gcaaataca 
      agaatcttc aagttgaaa ttcacactt ttaaccagt 
      cgtgttatc ccagaatca atagggata ctctaatgc 
      ctatcacga agaaaatcg atattcata acgacctgg 
      aatcgtggt atcctccag gtcacggcg atttttacc 
      aaagtttca aaaattcag gcctcttgc gcaaattca 
      tacaagaag ggcgggaat attgcttta tttctaaca 
      tcgataatc tgggagcta cagttgact tgaatattc 
      ttaacatgg tactgaatc cgaaagata aagtccaac 
      atgaattcg ttatggaag tcaccgata agactaggg 
      ctgacgtca agggcggta ctttgatcc aatacgaga 
      acaaactgc gcttattgg aaatcgccc aagtgccca 
      aagagcacg tcgacgact tcaagtcgg tcaaaacgt 
      tcaaatttt tcaatacga ataacttgt gggcaaaac 
      ttgacgcta ttgagcgag tcttagctc agggttcgc 
      ttagtcttg agattatta tcaataata aaagtctga 
      acaatggtt tgaatgtga ttcagttgg agacggctg 
      ttggggcgg cgatgaaat cgtttgagg caggcattg 
      gcataaatg tgccgcgga gtagatttt tgccggtga 
      aaaaaacac aggacttgc ttctagtca tgagtaatt 
      tgtacagtt tgaaaaatg ggtcgttgg tgatgtcgc 
      cacaaagga tgttcccca ctacgccac tggttaagt 
      taggggata atcatttcg cgaaagtga aggactttt 
      tggggcgct ttgcgacga ttcccgatt tgatcgagt 
      tggaccatt tgaccgttt cgggggatg ttacgtttg 
      gcaggggcg tctcgttga agggtactg taattataa 
      tcgcgaatc acggagaac ggatcgata ttccgtcag 
      gtgccaatt tagaaaata agatagtgt ccggtaata 
      tgcggattc tggatcatt gagtaacga gtatgcctg 
      tgtagattt tgttgtttt atttgtata tgttgttat 
      agtttttct acacgtaaa tttacagta actgtagtc 
      attgtttgc tatttattt atgaagatt tattgccta 
      gttttacaa taaatatat tttcggcat atttttggg 
      attttgctt ttaacgtag gcaaccaac aatacaaac 
      atacccttt ctaacaata ccaccttgt cctaccacc 
      actttttat ggcggcgtt ttgattaat taattaatg 
      atgcaataa taaattgaa aaccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY