Status
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Gene information
General information
TC009812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8505406..8508695
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009812
    iBeetle screen iB_04707

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009812 RA
      MSCGAGRGR GWLNLGSNA EPPKPMGLP PPVVPSPVE 
      VSPEYKELV DRVKELNVN DDGILLNQK VKHIQSFWQ 
      NECHTQEEV QASFEQLYN AALHDSELA SQLVTLVSL 
      RSSDSKTHD CNIRYEFLK RLQRDFQNS NQLQTTNPS 
      VFRNCMLII GEFFSKARL SDGEPYKFL ITPFFSCLE 
      LLLESKALP DLILFTRQL HLNGSTLRS RVPEDLTKL 
      TLKIRTLLA EPQLVKEAR LWLLLALDL CNVRFGVLP 
      NEIFKFYED QLGIVPMIN FQRPHDPLS IQTVHSSKV 
      LDSYQSNVN VLQISTSPT EPPNDVQQF GNLEYNGTQ 
      HKKDKPGRP ILGVGARFI NKNKDENGS WNNKKSLSG 
      RAPKKTPPD FKNKGWEHD DRFENDYS
    • >TC009812 RB
      MSCGAGRGR GWLNLGSNA EPPKPMGLP PPVVPSPVE 
      VSPEYKELV DRVKELNVN DDGILLNQK VKHIQSFWQ 
      NECHTQEEV QASFEQLYN AALHDSELA SQLVTLVSL 
      RSSDSKTHD CNIRYEFLK RLQRDFQNS NQLQTTNPS 
      VFRNCMLII GEFFSKARL SDGEPYKFL ITPFFSCLE 
      LLLESKALP DLILFTRQR PHDPLSIQT VHSSKVLDS 
      YQSNVNVLQ ISTSPTEPP NDVQQFGNL EYNGTQHKK 
      DKPGRPILG VGARFINKN KDENGSWNN KKSLSGRAP 
      KKTPPDFKN KGWEHDDRF ENDYS
    CDS sequence
    • >TC009812 RA
      atgtcgtgt ggggccggt cgaggcagg ggttggttg 
      aatttgggc tcgaatgcg gagcccccg aagcccatg 
      gggctgccc ccgccggtt gtcccatcg ccggtggag 
      gtgagcccc gagtacaag gaactggtg gacagagtc 
      aaggaactg aacgtgaac gatgatggg attcttttg 
      aatcagaaa gttaagcat attcagagc ttttggcag 
      aacgagtgt cacactcag gaggaagtt caggcgagt 
      ttcgagcag ttgtacaac gcagcgctt cacgattcg 
      gaattggca tcacagttg gtaactcta gtctcattg 
      aggtcttct gactcaaaa acgcatgat tgtaatata 
      agatacgag tttttgaag cggttgcag agggatttt 
      cagaatagt aatcagttg caaacaact aatccctca 
      gtgttcagg aactgtatg ctcattata ggagaattt 
      ttcagtaaa gctagatta tcggatggg gaaccttac 
      aagttttta ataacaccg tttttttcg tgcctagag 
      ttattacta gagagcaaa gctctacct gatctcatt 
      ttattcaca agacaatta cacttaaat ggctcaact 
      ttgagaagt cgagtgcct gaagacctt acaaagcta 
      acacttaaa ataagaaca ttattagca gaaccccaa 
      cttgtgaaa gaggcaaga ttatggctc ttgttggct 
      ttagaccta tgtaatgtc cgattcggg gttctgcca 
      aatgaaatt tttaaattt tatgaagat caactcgga 
      attgtgccc atgatcaac ttccagagg ccgcacgac 
      cctctcagc atccaaact gtgcactcg agcaaagtc 
      cttgacagt taccagagc aacgtaaac gtgttgcaa 
      atttcaacc tcccccacc gagccccca aatgacgtc 
      caacaattt ggcaacctt gagtacaac ggtactcag 
      cacaagaaa gacaagcct gggcgacct attctaggc 
      gtgggtgct aggtttata aataaaaat aaggatgaa 
      aacggttct tggaataat aagaaaagc ttgtctgga 
      agagcacct aagaaaacc ccacctgat tttaaaaat 
      aaagggtgg gaacatgat gacagattc gagaatgat 
      tatagttaa
    • >TC009812 RB
      atgtcgtgt ggggccggt cgaggcagg ggttggttg 
      aatttgggc tcgaatgcg gagcccccg aagcccatg 
      gggctgccc ccgccggtt gtcccatcg ccggtggag 
      gtgagcccc gagtacaag gaactggtg gacagagtc 
      aaggaactg aacgtgaac gatgatggg attcttttg 
      aatcagaaa gttaagcat attcagagc ttttggcag 
      aacgagtgt cacactcag gaggaagtt caggcgagt 
      ttcgagcag ttgtacaac gcagcgctt cacgattcg 
      gaattggca tcacagttg gtaactcta gtctcattg 
      aggtcttct gactcaaaa acgcatgat tgtaatata 
      agatacgag tttttgaag cggttgcag agggatttt 
      cagaatagt aatcagttg caaacaact aatccctca 
      gtgttcagg aactgtatg ctcattata ggagaattt 
      ttcagtaaa gctagatta tcggatggg gaaccttac 
      aagttttta ataacaccg tttttttcg tgcctagag 
      ttattacta gagagcaaa gctctacct gatctcatt 
      ttattcaca agacaaagg ccgcacgac cctctcagc 
      atccaaact gtgcactcg agcaaagtc cttgacagt 
      taccagagc aacgtaaac gtgttgcaa atttcaacc 
      tcccccacc gagccccca aatgacgtc caacaattt 
      ggcaacctt gagtacaac ggtactcag cacaagaaa 
      gacaagcct gggcgacct attctaggc gtgggtgct 
      aggtttata aataaaaat aaggatgaa aacggttct 
      tggaataat aagaaaagc ttgtctgga agagcacct 
      aagaaaacc ccacctgat tttaaaaat aaagggtgg 
      gaacatgat gacagattc gagaatgat tatagttaa
    mRNA sequence
    • >TC009812 RA
      tttttgtgg acgatttga caatttggt gcaaccctg 
      gttgcattg gcaacccgt ttgcagcgt tgccagatc 
      gtgcggtca gcgaaatgt cgtgtgggg ccggtcgag 
      gcaggggtt ggttgaatt tgggctcga atgcggagc 
      ccccgaagc ccatggggc tgcccccgc cggttgtcc 
      catcgccgg tggaggtga gccccgagt acaaggaac 
      tggtggaca gagtcaagg aactgaacg tgaacgatg 
      atgggattc ttttgaatc agaaagtta agcatattc 
      agagctttt ggcagaacg agtgtcaca ctcaggagg 
      aagttcagg cgagtttcg agcagttgt acaacgcag 
      cgcttcacg attcggaat tggcatcac agttggtaa 
      ctctagtct cattgaggt cttctgact caaaaacgc 
      atgattgta atataagat acgagtttt tgaagcggt 
      tgcagaggg attttcaga atagtaatc agttgcaaa 
      caactaatc cctcagtgt tcaggaact gtatgctca 
      ttataggag aatttttca gtaaagcta gattatcgg 
      atggggaac cttacaagt ttttaataa caccgtttt 
      tttcgtgcc tagagttat tactagaga gcaaagctc 
      tacctgatc tcattttat tcacaagac aattacact 
      taaatggct caactttga gaagtcgag tgcctgaag 
      accttacaa agctaacac ttaaaataa gaacattat 
      tagcagaac cccaacttg tgaaagagg caagattat 
      ggctcttgt tggctttag acctatgta atgtccgat 
      tcggggttc tgccaaatg aaattttta aattttatg 
      aagatcaac tcggaattg tgcccatga tcaacttcc 
      agaggccgc acgaccctc tcagcatcc aaactgtgc 
      actcgagca aagtccttg acagttacc agagcaacg 
      taaacgtgt tgcaaattt caacctccc ccaccgagc 
      ccccaaatg acgtccaac aatttggca accttgagt 
      acaacggta ctcagcaca agaaagaca agcctgggc 
      gacctattc taggcgtgg gtgctaggt ttataaata 
      aaaataagg atgaaaacg gttcttgga ataataaga 
      aaagcttgt ctggaagag cacctaaga aaaccccac 
      ctgatttta aaaataaag ggtgggaac atgatgaca 
      gattcgaga atgattata gttaaaaag tgatttgtt 
      tttattagg ttttggcaa ataatttta gtcaatttt 
      agtttttgc acttagtga tattcatga cgttctaaa 
      gaacgctca taaatactc tagttaagt gatcttcaa 
      ttaggtgaa tttttaaaa aggttttta tctcgatag 
      ttgtaattt ttatatcat gtagatgta tgtaatcct 
      ttatattta tattagtat ggtgtgtat tgtggttga 
      cattttatg tatattttt gagttttat tttgtgtac 
      catttttac gttaaaagc tgaggctta taatatttt 
      attgacgaa ttttacata aaaataatt gattaattt 
      tattgcata cagaaatat aagcctgaa gcactgttg 
      tctgaacaa aaaacctat aatttatgg tgcaaaggt 
      aatttttta aataagccg agttgtgta catgattat 
      catgttatg aattgaaaa ataaagaga gaacagtat 
      t
    • >TC009812 RB
      tttttgtgg acgatttga caatttggt gcaaccctg 
      gttgcattg gcaacccgt ttgcagcgt tgccagatc 
      gtgcggtca ggtttgttt gacccgtct ttcgtcatg 
      tgtttgttt attttttga ttttctcca gcgaaatgt 
      cgtgtgggg ccggtcgag gcaggggtt ggttgaatt 
      tgggctcga atgcggagc ccccgaagc ccatggggc 
      tgcccccgc cggttgtcc catcgccgg tggaggtga 
      gccccgagt acaaggaac tggtggaca gagtcaagg 
      aactgaacg tgaacgatg atgggattc ttttgaatc 
      agaaagtta agcatattc agagctttt ggcagaacg 
      agtgtcaca ctcaggagg aagttcagg cgagtttcg 
      agcagttgt acaacgcag cgcttcacg attcggaat 
      tggcatcac agttggtaa ctctagtct cattgaggt 
      cttctgact caaaaacgc atgattgta atataagat 
      acgagtttt tgaagcggt tgcagaggg attttcaga 
      atagtaatc agttgcaaa caactaatc cctcagtgt 
      tcaggaact gtatgctca ttataggag aatttttca 
      gtaaagcta gattatcgg atggggaac cttacaagt 
      ttttaataa caccgtttt tttcgtgcc tagagttat 
      tactagaga gcaaagctc tacctgatc tcattttat 
      tcacaagac aaaggccgc acgaccctc tcagcatcc 
      aaactgtgc actcgagca aagtccttg acagttacc 
      agagcaacg taaacgtgt tgcaaattt caacctccc 
      ccaccgagc ccccaaatg acgtccaac aatttggca 
      accttgagt acaacggta ctcagcaca agaaagaca 
      agcctgggc gacctattc taggcgtgg gtgctaggt 
      ttataaata aaaataagg atgaaaacg gttcttgga 
      ataataaga aaagcttgt ctggaagag cacctaaga 
      aaaccccac ctgatttta aaaataaag ggtgggaac 
      atgatgaca gattcgaga atgattata gttaaaaag 
      tgatttgtt tttattagg ttttggcaa ataatttta 
      gtcaatttt agtttttgc acttagtga tattcatga 
      cgttctaaa gaacgctca taaatactc tagttaagt 
      gatcttcaa ttaggtgaa tttttaaaa aggttttta 
      tctcgatag ttgtaattt ttatatcat gtagatgta 
      tgtaatcct ttatattta tattagtat ggtgtgtat 
      tgtggttga cattttatg tatattttt gagttttat 
      tttgtgtac catttttac gttaaaagc tgaggctta 
      taatatttt attgacgaa ttttacata aaaataatt 
      gattaattt tattgcata cagaaatat aagcctgaa 
      gcactgttg tctgaacaa aaaacctat aatttatgg 
      tgcaaaggt aatttttta aataagccg agttgtgta 
      catgattat catgttatg aattgaaaa ataaagaga 
      gaacagtat t
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