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Gene information
General information
TC009812 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8505406..8508695
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009812
    iBeetle screen iB_04707

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009812 RA
      MSCGAGRGR GWLNLGSNA EPPKPMGLP PPVVPSPVE 
VSPEYKELV DRVKELNVN DDGILLNQK VKHIQSFWQ 
NECHTQEEV QASFEQLYN AALHDSELA SQLVTLVSL 
RSSDSKTHD CNIRYEFLK RLQRDFQNS NQLQTTNPS 
VFRNCMLII GEFFSKARL SDGEPYKFL ITPFFSCLE 
LLLESKALP DLILFTRQL HLNGSTLRS RVPEDLTKL 
TLKIRTLLA EPQLVKEAR LWLLLALDL CNVRFGVLP 
NEIFKFYED QLGIVPMIN FQRPHDPLS IQTVHSSKV 
LDSYQSNVN VLQISTSPT EPPNDVQQF GNLEYNGTQ 
HKKDKPGRP ILGVGARFI NKNKDENGS WNNKKSLSG 
RAPKKTPPD FKNKGWEHD DRFENDYS
    • >TC009812 RB
      MSCGAGRGR GWLNLGSNA EPPKPMGLP PPVVPSPVE 
VSPEYKELV DRVKELNVN DDGILLNQK VKHIQSFWQ 
NECHTQEEV QASFEQLYN AALHDSELA SQLVTLVSL 
RSSDSKTHD CNIRYEFLK RLQRDFQNS NQLQTTNPS 
VFRNCMLII GEFFSKARL SDGEPYKFL ITPFFSCLE 
LLLESKALP DLILFTRQR PHDPLSIQT VHSSKVLDS 
YQSNVNVLQ ISTSPTEPP NDVQQFGNL EYNGTQHKK 
DKPGRPILG VGARFINKN KDENGSWNN KKSLSGRAP 
KKTPPDFKN KGWEHDDRF ENDYS
    CDS sequence
    • >TC009812 RA
      atgtcgtgt ggggccggt cgaggcagg ggttggttg 
aatttgggc tcgaatgcg gagcccccg aagcccatg 
gggctgccc ccgccggtt gtcccatcg ccggtggag 
gtgagcccc gagtacaag gaactggtg gacagagtc 
aaggaactg aacgtgaac gatgatggg attcttttg 
aatcagaaa gttaagcat attcagagc ttttggcag 
aacgagtgt cacactcag gaggaagtt caggcgagt 
ttcgagcag ttgtacaac gcagcgctt cacgattcg 
gaattggca tcacagttg gtaactcta gtctcattg 
aggtcttct gactcaaaa acgcatgat tgtaatata 
agatacgag tttttgaag cggttgcag agggatttt 
cagaatagt aatcagttg caaacaact aatccctca 
gtgttcagg aactgtatg ctcattata ggagaattt 
ttcagtaaa gctagatta tcggatggg gaaccttac 
aagttttta ataacaccg tttttttcg tgcctagag 
ttattacta gagagcaaa gctctacct gatctcatt 
ttattcaca agacaatta cacttaaat ggctcaact 
ttgagaagt cgagtgcct gaagacctt acaaagcta 
acacttaaa ataagaaca ttattagca gaaccccaa 
cttgtgaaa gaggcaaga ttatggctc ttgttggct 
ttagaccta tgtaatgtc cgattcggg gttctgcca 
aatgaaatt tttaaattt tatgaagat caactcgga 
attgtgccc atgatcaac ttccagagg ccgcacgac 
cctctcagc atccaaact gtgcactcg agcaaagtc 
cttgacagt taccagagc aacgtaaac gtgttgcaa 
atttcaacc tcccccacc gagccccca aatgacgtc 
caacaattt ggcaacctt gagtacaac ggtactcag 
cacaagaaa gacaagcct gggcgacct attctaggc 
gtgggtgct aggtttata aataaaaat aaggatgaa 
aacggttct tggaataat aagaaaagc ttgtctgga 
agagcacct aagaaaacc ccacctgat tttaaaaat 
aaagggtgg gaacatgat gacagattc gagaatgat 
tatagttaa
    • >TC009812 RB
      atgtcgtgt ggggccggt cgaggcagg ggttggttg 
aatttgggc tcgaatgcg gagcccccg aagcccatg 
gggctgccc ccgccggtt gtcccatcg ccggtggag 
gtgagcccc gagtacaag gaactggtg gacagagtc 
aaggaactg aacgtgaac gatgatggg attcttttg 
aatcagaaa gttaagcat attcagagc ttttggcag 
aacgagtgt cacactcag gaggaagtt caggcgagt 
ttcgagcag ttgtacaac gcagcgctt cacgattcg 
gaattggca tcacagttg gtaactcta gtctcattg 
aggtcttct gactcaaaa acgcatgat tgtaatata 
agatacgag tttttgaag cggttgcag agggatttt 
cagaatagt aatcagttg caaacaact aatccctca 
gtgttcagg aactgtatg ctcattata ggagaattt 
ttcagtaaa gctagatta tcggatggg gaaccttac 
aagttttta ataacaccg tttttttcg tgcctagag 
ttattacta gagagcaaa gctctacct gatctcatt 
ttattcaca agacaaagg ccgcacgac cctctcagc 
atccaaact gtgcactcg agcaaagtc cttgacagt 
taccagagc aacgtaaac gtgttgcaa atttcaacc 
tcccccacc gagccccca aatgacgtc caacaattt 
ggcaacctt gagtacaac ggtactcag cacaagaaa 
gacaagcct gggcgacct attctaggc gtgggtgct 
aggtttata aataaaaat aaggatgaa aacggttct 
tggaataat aagaaaagc ttgtctgga agagcacct 
aagaaaacc ccacctgat tttaaaaat aaagggtgg 
gaacatgat gacagattc gagaatgat tatagttaa
    mRNA sequence
    • >TC009812 RA
      tttttgtgg acgatttga caatttggt gcaaccctg 
gttgcattg gcaacccgt ttgcagcgt tgccagatc 
gtgcggtca gcgaaatgt cgtgtgggg ccggtcgag 
gcaggggtt ggttgaatt tgggctcga atgcggagc 
ccccgaagc ccatggggc tgcccccgc cggttgtcc 
catcgccgg tggaggtga gccccgagt acaaggaac 
tggtggaca gagtcaagg aactgaacg tgaacgatg 
atgggattc ttttgaatc agaaagtta agcatattc 
agagctttt ggcagaacg agtgtcaca ctcaggagg 
aagttcagg cgagtttcg agcagttgt acaacgcag 
cgcttcacg attcggaat tggcatcac agttggtaa 
ctctagtct cattgaggt cttctgact caaaaacgc 
atgattgta atataagat acgagtttt tgaagcggt 
tgcagaggg attttcaga atagtaatc agttgcaaa 
caactaatc cctcagtgt tcaggaact gtatgctca 
ttataggag aatttttca gtaaagcta gattatcgg 
atggggaac cttacaagt ttttaataa caccgtttt 
tttcgtgcc tagagttat tactagaga gcaaagctc 
tacctgatc tcattttat tcacaagac aattacact 
taaatggct caactttga gaagtcgag tgcctgaag 
accttacaa agctaacac ttaaaataa gaacattat 
tagcagaac cccaacttg tgaaagagg caagattat 
ggctcttgt tggctttag acctatgta atgtccgat 
tcggggttc tgccaaatg aaattttta aattttatg 
aagatcaac tcggaattg tgcccatga tcaacttcc 
agaggccgc acgaccctc tcagcatcc aaactgtgc 
actcgagca aagtccttg acagttacc agagcaacg 
taaacgtgt tgcaaattt caacctccc ccaccgagc 
ccccaaatg acgtccaac aatttggca accttgagt 
acaacggta ctcagcaca agaaagaca agcctgggc 
gacctattc taggcgtgg gtgctaggt ttataaata 
aaaataagg atgaaaacg gttcttgga ataataaga 
aaagcttgt ctggaagag cacctaaga aaaccccac 
ctgatttta aaaataaag ggtgggaac atgatgaca 
gattcgaga atgattata gttaaaaag tgatttgtt 
tttattagg ttttggcaa ataatttta gtcaatttt 
agtttttgc acttagtga tattcatga cgttctaaa 
gaacgctca taaatactc tagttaagt gatcttcaa 
ttaggtgaa tttttaaaa aggttttta tctcgatag 
ttgtaattt ttatatcat gtagatgta tgtaatcct 
ttatattta tattagtat ggtgtgtat tgtggttga 
cattttatg tatattttt gagttttat tttgtgtac 
catttttac gttaaaagc tgaggctta taatatttt 
attgacgaa ttttacata aaaataatt gattaattt 
tattgcata cagaaatat aagcctgaa gcactgttg 
tctgaacaa aaaacctat aatttatgg tgcaaaggt 
aatttttta aataagccg agttgtgta catgattat 
catgttatg aattgaaaa ataaagaga gaacagtat 
t
    • >TC009812 RB
      tttttgtgg acgatttga caatttggt gcaaccctg 
gttgcattg gcaacccgt ttgcagcgt tgccagatc 
gtgcggtca ggtttgttt gacccgtct ttcgtcatg 
tgtttgttt attttttga ttttctcca gcgaaatgt 
cgtgtgggg ccggtcgag gcaggggtt ggttgaatt 
tgggctcga atgcggagc ccccgaagc ccatggggc 
tgcccccgc cggttgtcc catcgccgg tggaggtga 
gccccgagt acaaggaac tggtggaca gagtcaagg 
aactgaacg tgaacgatg atgggattc ttttgaatc 
agaaagtta agcatattc agagctttt ggcagaacg 
agtgtcaca ctcaggagg aagttcagg cgagtttcg 
agcagttgt acaacgcag cgcttcacg attcggaat 
tggcatcac agttggtaa ctctagtct cattgaggt 
cttctgact caaaaacgc atgattgta atataagat 
acgagtttt tgaagcggt tgcagaggg attttcaga 
atagtaatc agttgcaaa caactaatc cctcagtgt 
tcaggaact gtatgctca ttataggag aatttttca 
gtaaagcta gattatcgg atggggaac cttacaagt 
ttttaataa caccgtttt tttcgtgcc tagagttat 
tactagaga gcaaagctc tacctgatc tcattttat 
tcacaagac aaaggccgc acgaccctc tcagcatcc 
aaactgtgc actcgagca aagtccttg acagttacc 
agagcaacg taaacgtgt tgcaaattt caacctccc 
ccaccgagc ccccaaatg acgtccaac aatttggca 
accttgagt acaacggta ctcagcaca agaaagaca 
agcctgggc gacctattc taggcgtgg gtgctaggt 
ttataaata aaaataagg atgaaaacg gttcttgga 
ataataaga aaagcttgt ctggaagag cacctaaga 
aaaccccac ctgatttta aaaataaag ggtgggaac 
atgatgaca gattcgaga atgattata gttaaaaag 
tgatttgtt tttattagg ttttggcaa ataatttta 
gtcaatttt agtttttgc acttagtga tattcatga 
cgttctaaa gaacgctca taaatactc tagttaagt 
gatcttcaa ttaggtgaa tttttaaaa aggttttta 
tctcgatag ttgtaattt ttatatcat gtagatgta 
tgtaatcct ttatattta tattagtat ggtgtgtat 
tgtggttga cattttatg tatattttt gagttttat 
tttgtgtac catttttac gttaaaagc tgaggctta 
taatatttt attgacgaa ttttacata aaaataatt 
gattaattt tattgcata cagaaatat aagcctgaa 
gcactgttg tctgaacaa aaaacctat aatttatgg 
tgcaaaggt aatttttta aataagccg agttgtgta 
catgattat catgttatg aattgaaaa ataaagaga 
gaacagtat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY