| General information | |||
| TC009812 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8505406..8508695 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009812 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04707 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC009812 RA
MSCGAGRGR GWLNLGSNA EPPKPMGLP PPVVPSPVE VSPEYKELV DRVKELNVN DDGILLNQK VKHIQSFWQ NECHTQEEV QASFEQLYN AALHDSELA SQLVTLVSL RSSDSKTHD CNIRYEFLK RLQRDFQNS NQLQTTNPS VFRNCMLII GEFFSKARL SDGEPYKFL ITPFFSCLE LLLESKALP DLILFTRQL HLNGSTLRS RVPEDLTKL TLKIRTLLA EPQLVKEAR LWLLLALDL CNVRFGVLP NEIFKFYED QLGIVPMIN FQRPHDPLS IQTVHSSKV LDSYQSNVN VLQISTSPT EPPNDVQQF GNLEYNGTQ HKKDKPGRP ILGVGARFI NKNKDENGS WNNKKSLSG RAPKKTPPD FKNKGWEHD DRFENDYS
- >TC009812 RB
MSCGAGRGR GWLNLGSNA EPPKPMGLP PPVVPSPVE VSPEYKELV DRVKELNVN DDGILLNQK VKHIQSFWQ NECHTQEEV QASFEQLYN AALHDSELA SQLVTLVSL RSSDSKTHD CNIRYEFLK RLQRDFQNS NQLQTTNPS VFRNCMLII GEFFSKARL SDGEPYKFL ITPFFSCLE LLLESKALP DLILFTRQR PHDPLSIQT VHSSKVLDS YQSNVNVLQ ISTSPTEPP NDVQQFGNL EYNGTQHKK DKPGRPILG VGARFINKN KDENGSWNN KKSLSGRAP KKTPPDFKN KGWEHDDRF ENDYS
- >TC009812 RA
atgtcgtgt ggggccggt cgaggcagg ggttggttg aatttgggc tcgaatgcg gagcccccg aagcccatg gggctgccc ccgccggtt gtcccatcg ccggtggag gtgagcccc gagtacaag gaactggtg gacagagtc aaggaactg aacgtgaac gatgatggg attcttttg aatcagaaa gttaagcat attcagagc ttttggcag aacgagtgt cacactcag gaggaagtt caggcgagt ttcgagcag ttgtacaac gcagcgctt cacgattcg gaattggca tcacagttg gtaactcta gtctcattg aggtcttct gactcaaaa acgcatgat tgtaatata agatacgag tttttgaag cggttgcag agggatttt cagaatagt aatcagttg caaacaact aatccctca gtgttcagg aactgtatg ctcattata ggagaattt ttcagtaaa gctagatta tcggatggg gaaccttac aagttttta ataacaccg tttttttcg tgcctagag ttattacta gagagcaaa gctctacct gatctcatt ttattcaca agacaatta cacttaaat ggctcaact ttgagaagt cgagtgcct gaagacctt acaaagcta acacttaaa ataagaaca ttattagca gaaccccaa cttgtgaaa gaggcaaga ttatggctc ttgttggct ttagaccta tgtaatgtc cgattcggg gttctgcca aatgaaatt tttaaattt tatgaagat caactcgga attgtgccc atgatcaac ttccagagg ccgcacgac cctctcagc atccaaact gtgcactcg agcaaagtc cttgacagt taccagagc aacgtaaac gtgttgcaa atttcaacc tcccccacc gagccccca aatgacgtc caacaattt ggcaacctt gagtacaac ggtactcag cacaagaaa gacaagcct gggcgacct attctaggc gtgggtgct aggtttata aataaaaat aaggatgaa aacggttct tggaataat aagaaaagc ttgtctgga agagcacct aagaaaacc ccacctgat tttaaaaat aaagggtgg gaacatgat gacagattc gagaatgat tatagttaa
- >TC009812 RB
atgtcgtgt ggggccggt cgaggcagg ggttggttg aatttgggc tcgaatgcg gagcccccg aagcccatg gggctgccc ccgccggtt gtcccatcg ccggtggag gtgagcccc gagtacaag gaactggtg gacagagtc aaggaactg aacgtgaac gatgatggg attcttttg aatcagaaa gttaagcat attcagagc ttttggcag aacgagtgt cacactcag gaggaagtt caggcgagt ttcgagcag ttgtacaac gcagcgctt cacgattcg gaattggca tcacagttg gtaactcta gtctcattg aggtcttct gactcaaaa acgcatgat tgtaatata agatacgag tttttgaag cggttgcag agggatttt cagaatagt aatcagttg caaacaact aatccctca gtgttcagg aactgtatg ctcattata ggagaattt ttcagtaaa gctagatta tcggatggg gaaccttac aagttttta ataacaccg tttttttcg tgcctagag ttattacta gagagcaaa gctctacct gatctcatt ttattcaca agacaaagg ccgcacgac cctctcagc atccaaact gtgcactcg agcaaagtc cttgacagt taccagagc aacgtaaac gtgttgcaa atttcaacc tcccccacc gagccccca aatgacgtc caacaattt ggcaacctt gagtacaac ggtactcag cacaagaaa gacaagcct gggcgacct attctaggc gtgggtgct aggtttata aataaaaat aaggatgaa aacggttct tggaataat aagaaaagc ttgtctgga agagcacct aagaaaacc ccacctgat tttaaaaat aaagggtgg gaacatgat gacagattc gagaatgat tatagttaa
- >TC009812 RA
tttttgtgg acgatttga caatttggt gcaaccctg gttgcattg gcaacccgt ttgcagcgt tgccagatc gtgcggtca gcgaaatgt cgtgtgggg ccggtcgag gcaggggtt ggttgaatt tgggctcga atgcggagc ccccgaagc ccatggggc tgcccccgc cggttgtcc catcgccgg tggaggtga gccccgagt acaaggaac tggtggaca gagtcaagg aactgaacg tgaacgatg atgggattc ttttgaatc agaaagtta agcatattc agagctttt ggcagaacg agtgtcaca ctcaggagg aagttcagg cgagtttcg agcagttgt acaacgcag cgcttcacg attcggaat tggcatcac agttggtaa ctctagtct cattgaggt cttctgact caaaaacgc atgattgta atataagat acgagtttt tgaagcggt tgcagaggg attttcaga atagtaatc agttgcaaa caactaatc cctcagtgt tcaggaact gtatgctca ttataggag aatttttca gtaaagcta gattatcgg atggggaac cttacaagt ttttaataa caccgtttt tttcgtgcc tagagttat tactagaga gcaaagctc tacctgatc tcattttat tcacaagac aattacact taaatggct caactttga gaagtcgag tgcctgaag accttacaa agctaacac ttaaaataa gaacattat tagcagaac cccaacttg tgaaagagg caagattat ggctcttgt tggctttag acctatgta atgtccgat tcggggttc tgccaaatg aaattttta aattttatg aagatcaac tcggaattg tgcccatga tcaacttcc agaggccgc acgaccctc tcagcatcc aaactgtgc actcgagca aagtccttg acagttacc agagcaacg taaacgtgt tgcaaattt caacctccc ccaccgagc ccccaaatg acgtccaac aatttggca accttgagt acaacggta ctcagcaca agaaagaca agcctgggc gacctattc taggcgtgg gtgctaggt ttataaata aaaataagg atgaaaacg gttcttgga ataataaga aaagcttgt ctggaagag cacctaaga aaaccccac ctgatttta aaaataaag ggtgggaac atgatgaca gattcgaga atgattata gttaaaaag tgatttgtt tttattagg ttttggcaa ataatttta gtcaatttt agtttttgc acttagtga tattcatga cgttctaaa gaacgctca taaatactc tagttaagt gatcttcaa ttaggtgaa tttttaaaa aggttttta tctcgatag ttgtaattt ttatatcat gtagatgta tgtaatcct ttatattta tattagtat ggtgtgtat tgtggttga cattttatg tatattttt gagttttat tttgtgtac catttttac gttaaaagc tgaggctta taatatttt attgacgaa ttttacata aaaataatt gattaattt tattgcata cagaaatat aagcctgaa gcactgttg tctgaacaa aaaacctat aatttatgg tgcaaaggt aatttttta aataagccg agttgtgta catgattat catgttatg aattgaaaa ataaagaga gaacagtat t
- >TC009812 RB
tttttgtgg acgatttga caatttggt gcaaccctg gttgcattg gcaacccgt ttgcagcgt tgccagatc gtgcggtca ggtttgttt gacccgtct ttcgtcatg tgtttgttt attttttga ttttctcca gcgaaatgt cgtgtgggg ccggtcgag gcaggggtt ggttgaatt tgggctcga atgcggagc ccccgaagc ccatggggc tgcccccgc cggttgtcc catcgccgg tggaggtga gccccgagt acaaggaac tggtggaca gagtcaagg aactgaacg tgaacgatg atgggattc ttttgaatc agaaagtta agcatattc agagctttt ggcagaacg agtgtcaca ctcaggagg aagttcagg cgagtttcg agcagttgt acaacgcag cgcttcacg attcggaat tggcatcac agttggtaa ctctagtct cattgaggt cttctgact caaaaacgc atgattgta atataagat acgagtttt tgaagcggt tgcagaggg attttcaga atagtaatc agttgcaaa caactaatc cctcagtgt tcaggaact gtatgctca ttataggag aatttttca gtaaagcta gattatcgg atggggaac cttacaagt ttttaataa caccgtttt tttcgtgcc tagagttat tactagaga gcaaagctc tacctgatc tcattttat tcacaagac aaaggccgc acgaccctc tcagcatcc aaactgtgc actcgagca aagtccttg acagttacc agagcaacg taaacgtgt tgcaaattt caacctccc ccaccgagc ccccaaatg acgtccaac aatttggca accttgagt acaacggta ctcagcaca agaaagaca agcctgggc gacctattc taggcgtgg gtgctaggt ttataaata aaaataagg atgaaaacg gttcttgga ataataaga aaagcttgt ctggaagag cacctaaga aaaccccac ctgatttta aaaataaag ggtgggaac atgatgaca gattcgaga atgattata gttaaaaag tgatttgtt tttattagg ttttggcaa ataatttta gtcaatttt agtttttgc acttagtga tattcatga cgttctaaa gaacgctca taaatactc tagttaagt gatcttcaa ttaggtgaa tttttaaaa aggttttta tctcgatag ttgtaattt ttatatcat gtagatgta tgtaatcct ttatattta tattagtat ggtgtgtat tgtggttga cattttatg tatattttt gagttttat tttgtgtac catttttac gttaaaagc tgaggctta taatatttt attgacgaa ttttacata aaaataatt gattaattt tattgcata cagaaatat aagcctgaa gcactgttg tctgaacaa aaaacctat aatttatgg tgcaaaggt aatttttta aataagccg agttgtgta catgattat catgttatg aattgaaaa ataaagaga gaacagtat t
