Status
Please wait ...
Gene information
General information
TC009798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8237380..8240502
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009798
    iBeetle screen iB_04705

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    prothorax sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009798 RA
      MSFLRGSQN YVWSTTSVL GKGATGAVY QGVNKINGE 
      PVAVKTFNQ MSHLRPHDV QMREFEVLQ KVKHENIVK 
      LLAIEEEQE NRGKVIVME LCTGGSLFN ILDDPENTY 
      GLAENEFLK VLQDLSAGM KHLRDNNLV HRDLKPGNI 
      MKFICDDGR TVYKLTDFG AARELPEGQ HFQSLYGTE 
      EYLHPDLYE RAVLRKHVN KTFGATIDL WSIGVTLYH 
      VATGNLPFR PYGGRKNKE TMHLITTKK ASGVISGIQ 
      VTEDGQIEW RKTLPESCQ LSPGLKKII TPLLAGLLE 
      ADTKKIWTF ERFFTEVTD MLSRKIVNI FHVNKAQLI 
      KVYIHPDES YNHLQNYIN EQTEVSLEN QILLLKSNL 
      FTDLVEENS RAGGYPETK EDEPLFLFN KENNNVTVI 
      PEQNLPKFT EFSNVTSVE SDAAQAKNA CSIGYLCKR 
      RIEKYSLAC SHFNNCVEN FVQYINKEL REMNQESLH 
      LLEKTTIHK KTAHFLESS QKIAAFKFS NIHQVVYAD 
      ELKHLSESF VSETAKKIM QLNQSHVIE NTLHLEWDV 
      SSRPLKSPV KSRGAERAR TEVERLRDS WQHLVRDRA 
      TRTLSYNDE QFHILERIK ITHTINRIK VLLQKEVFP 
      QYVQLAENL GDWYKIAQT VYLQMMILK RDVLNYDAN 
      LKKFELEMF IKNEEYLEQ VKKLLEQDA TDSSSKKMK 
      NSNNQKLKM CLNEYRREC CDLNEVVLE NEQLVEQVN 
      RILEELIVD EEG
    CDS sequence
    • >TC009798 RA
      atgtcgttt ttacgcgga tctcagaat tacgtctgg 
      tcgacaacg agtgtcttg ggcaagggg gccaccggg 
      gctgtctac caaggcgta aacaaaatc aatggtgaa 
      cctgtcgcg gtgaaaact ttcaatcaa atgagccac 
      ctgcgcccc cacgacgtg caaatgcgc gaattcgaa 
      gtgctccag aaagtgaaa catgaaaat atcgtcaaa 
      cttttggcc attgaggaa gaacaggaa aaccgtgga 
      aaggttatt gtgatggaa ctgtgtacg gggggaagt 
      cttttcaac attttggac gacccggaa aacacctac 
      ggactagcc gaaaatgag ttcctgaag gtgctccag 
      gacttgtcc gccgggatg aagcacctc cgggataat 
      aacctggtc caccgggat ttaaagcca ggtaacata 
      atgaaattt atctgtgat gatggccgg actgtgtac 
      aagttgacc gactttggg gctgcccga gaactcccc 
      gagggccaa catttccag tcattatat ggcaccgaa 
      gaatactta caccctgat ctctacgaa cgcgcagta 
      ttacggaaa cacgtcaat aaaaccttt ggcgccaca 
      attgacttg tggtcaatc ggagtcact ctgtatcat 
      gtcgccacc ggaaatctt ccattccgt ccatacggg 
      ggccgcaaa aacaaggaa acgatgcat ttaataacg 
      acgaaaaaa gcaagtggc gttatttcc ggaatacag 
      gtcactgaa gatggtcaa atagagtgg cgcaaaacg 
      ttgccggaa agttgccaa ttaagtccg ggtttaaaa 
      aaaataatc acgccgcta ttggccggt ttattggaa 
      gctgacacg aaaaaaatt tggacgttt gagcgcttt 
      ttcaccgaa gtcactgac atgttatcg cgaaaaatc 
      gttaatatt tttcacgtg aacaaagca cagctcatc 
      aaggtttac attcatccc gacgaaagc tacaatcac 
      ctacagaat tacataaac gaacagact gaagtatcg 
      cttgaaaat caaatcttg ctccttaaa tcaaattta 
      ttcaccgac ttagtggaa gaaaatagt cgagccggg 
      ggttaccct gaaacaaaa gaagacgaa cctttattc 
      ctgtttaat aaagaaaac aacaacgtg acggtaatc 
      cccgagcag aatttaccg aaatttacc gaatttagt 
      aatgtgact tcggttgaa agcgacgca gcgcaggcg 
      aaaaacgcc tgcagtatc ggctacctt tgcaaacgc 
      cgaatcgaa aaatattca ctcgcttgc tcccacttc 
      aacaactgt gttgaaaac ttcgtgcaa tacataaac 
      aaggaactc agagaaatg aaccaagaa tcgttacat 
      ctcctagag aaaaccacg attcataaa aagactgct 
      catttcctg gaatcatcg caaaaaatt gccgccttc 
      aagttcagc aatatccac caagtggtc tacgctgac 
      gagttgaaa cacctgagc gagagtttt gtctcagaa 
      acggcgaaa aaaatcatg caactgaac cagagccac 
      gtgatcgag aacacgttg catttggag tgggacgtg 
      tcaagtcgg ccgttgaag agtccggtg aagtcccga 
      ggggccgaa agggcccgc accgaagtg gagcgattg 
      agggactca tggcagcac ctcgtgcga gatcgcgcc 
      actcggacg ctttcgtac aacgacgag cagttccat 
      attttggaa cgcatcaaa atcacgcac acgattaat 
      cggatcaag gttttgctg cagaaggaa gtgtttccg 
      cagtatgtg caattggcg gagaatttg ggcgattgg 
      tataaaatt gcccaaacg gtttatttg caaatgatg 
      attttgaaa agggacgtt ttgaattat gacgccaat 
      ttgaagaag tttgagttg gagatgttt attaaaaat 
      gaggagtat ttggaacaa gtgaagaaa ttgttggaa 
      caggacgcg actgatagt tcgagcaag aagatgaag 
      aattcgaat aatcaaaag ttgaaaatg tgtctgaat 
      gagtatagg agggaatgt tgtgatttg aacgaagtt 
      gttttggaa aatgaacaa ctcgttgaa caagtgaat 
      aggattctg gaggagttg atcgttgac gaggagggc 
      tga
    mRNA sequence
    • >TC009798 RA
      cttcactgc tgtgacaca aatacagct gacgaaaca 
      tttcaagtt aattttttg tgaaatttc ggtaattca 
      ccgatgtga gaaagcatg tgacccaaa tatcggccc 
      ccaaaatgt cgtttttac gcggatctc agaattacg 
      tctggtcga caacgagtg tcttgggca agggggcca 
      ccggggctg tctaccaag gcgtaaaca aaatcaatg 
      gtgaacctg tcgcggtga aaactttca atcaaatga 
      gccacctgc gcccccacg acgtgcaaa tgcgcgaat 
      tcgaagtgc tccagaaag tgaaacatg aaaatatcg 
      tcaaacttt tggccattg aggaagaac aggaaaacc 
      gtggaaagg ttattgtga tggaactgt gtacggggg 
      gaagtcttt tcaacattt tggacgacc cggaaaaca 
      cctacggac tagccgaaa atgagttcc tgaaggtgc 
      tccaggact tgtccgccg ggatgaagc acctccggg 
      ataataacc tggtccacc gggatttaa agccaggta 
      acataatga aatttatct gtgatgatg gccggactg 
      tgtacaagt tgaccgact ttggggctg cccgagaac 
      tccccgagg gccaacatt tccagtcat tatatggca 
      ccgaagaat acttacacc ctgatctct acgaacgcg 
      cagtattac ggaaacacg tcaataaaa cctttggcg 
      ccacaattg acttgtggt caatcggag tcactctgt 
      atcatgtcg ccaccggaa atcttccat tccgtccat 
      acgggggcc gcaaaaaca aggaaacga tgcatttaa 
      taacgacga aaaaagcaa gtggcgtta tttccggaa 
      tacaggtca ctgaagatg gtcaaatag agtggcgca 
      aaacgttgc cggaaagtt gccaattaa gtccgggtt 
      taaaaaaaa taatcacgc cgctattgg ccggtttat 
      tggaagctg acacgaaaa aaatttgga cgtttgagc 
      gctttttca ccgaagtca ctgacatgt tatcgcgaa 
      aaatcgtta atatttttc acgtgaaca aagcacagc 
      tcatcaagg tttacattc atcccgacg aaagctaca 
      atcacctac agaattaca taaacgaac agactgaag 
      tatcgcttg aaaatcaaa tcttgctcc ttaaatcaa 
      atttattca ccgacttag tggaagaaa atagtcgag 
      ccgggggtt accctgaaa caaaagaag acgaacctt 
      tattcctgt ttaataaag aaaacaaca acgtgacgg 
      taatccccg agcagaatt taccgaaat ttaccgaat 
      ttagtaatg tgacttcgg ttgaaagcg acgcagcgc 
      aggcgaaaa acgcctgca gtatcggct acctttgca 
      aacgccgaa tcgaaaaat attcactcg cttgctccc 
      acttcaaca actgtgttg aaaacttcg tgcaataca 
      taaacaagg aactcagag aaatgaacc aagaatcgt 
      tacatctcc tagagaaaa ccacgattc ataaaaaga 
      ctgctcatt tcctggaat catcgcaaa aaattgccg 
      ccttcaagt tcagcaata tccaccaag tggtctacg 
      ctgacgagt tgaaacacc tgagcgaga gttttgtct 
      cagaaacgg cgaaaaaaa tcatgcaac tgaaccaga 
      gccacgtga tcgagaaca cgttgcatt tggagtggg 
      acgtgtcaa gtcggccgt tgaagagtc cggtgaagt 
      cccgagggg ccgaaaggg cccgcaccg aagtggagc 
      gattgaggg actcatggc agcacctcg tgcgagatc 
      gcgccactc ggacgcttt cgtacaacg acgagcagt 
      tccatattt tggaacgca tcaaaatca cgcacacga 
      ttaatcgga tcaaggttt tgctgcaga aggaagtgt 
      ttccgcagt atgtgcaat tggcggaga atttgggcg 
      attggtata aaattgccc aaacggttt atttgcaaa 
      tgatgattt tgaaaaggg acgttttga attatgacg 
      ccaatttga agaagtttg agttggaga tgtttatta 
      aaaatgagg agtatttgg aacaagtga agaaattgt 
      tggaacagg acgcgactg atagttcga gcaagaaga 
      tgaagaatt cgaataatc aaaagttga aaatgtgtc 
      tgaatgagt ataggaggg aatgttgtg atttgaacg 
      aagttgttt tggaaaatg aacaactcg ttgaacaag 
      tgaatagga ttctggagg agttgatcg ttgacgagg 
      agggctgat tgcattccg tgttctttt tgagattag 
      ttgccagtt ctcacctgc ttgtgttta caaaattgt 
      attatggat attgttata ctgaagaaa ttaaaatat 
      ttttacttg atttgtttt ctgttttgt aaatggaaa 
      aacatcaac agattaata aattttttg ttggtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY