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Gene information
General information
TC009795 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8215770..8217074
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009795
    iBeetle screen iB_04704

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment posterior randomnumber decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009795 RA
      MDHARFISV VKQFPQVWD RNCATFREQ SLKSEAWER 
      IGEIMNCPA SSCKDTFKS LREKYVRER EKQAMYADK 
      YMPWQHLES LSFLDPHII LRKQSLNYD SDMSKLPLD 
      ETKMNMTDE PFFVQLAEN YSDFDKTLI ALVRKASPI 
      WDRKSNGYP NKQVKYQLW RGIAQAMNK DINVCMMRW 
      KGLREKYIR QKNKYQEGE GKWEFLDDM SFLDKVIQY 
      RRKNCEPDM SFRPQSGPA EGFPFHNDM YDDNSMNSS 
      SNDFIPTVK SEDFGCDMQ DSSYDSGTA SSGRKRSAS 
      DNSESSYEK REKVEEIQK SPEAMFGEL VAAMLAKKT 
      ERDKNIAMI EIMKVLTK
    CDS sequence
    • >TC009795 RA
      atggatcat gccagattt attagtgtt gtcaagcag 
      ttcccccag gtgtgggac cgaaattgc gctactttc 
      agggagcag agcctgaaa agtgaagct tgggagaga 
      atcggcgaa attatgaat tgtccagct tcgtcctgt 
      aaagacacc ttcaagagt cttcgggag aagtatgtg 
      agagaaagg gaaaaacag gccatgtat gcggacaag 
      tacatgcct tggcagcac ctggagagc ctgtctttc 
      ctggacccc catattatc ctcaggaag cagtcgctg 
      aactatgac agtgacatg tcgaagcta cccctcgac 
      gagacaaaa atgaacatg acggacgag ccatttttc 
      gtccaactg gcggaaaat tacagcgat tttgacaag 
      actttgatc gcgttggtg cgcaaggcg agccccatt 
      tgggaccgc aagagcaac ggttacccc aacaagcag 
      gtgaagtac cagctgtgg cggggcata gcccaggcc 
      atgaacaag gatatcaac gtctgcatg atgcgctgg 
      aaggggctc agggagaag tacatccgc cagaaaaat 
      aagtaccaa gaaggggag ggcaagtgg gagtttctg 
      gatgacatg tcgttcctg gacaaggtg atccaatac 
      cggcgcaag aactgcgaa ccggatatg agctttcgc 
      ccccagagt ggccccgca gaggggttt ccgtttcac 
      aatgacatg tacgacgac aattcgatg aactcgtcg 
      tcaaacgat tttatacca actgttaaa agtgaggat 
      tttggatgt gatatgcag gatagttcc tacgattcc 
      gggacggcc agttccgga aggaagagg tcagcgagt 
      gataatagc gagtcgtcc tacgaaaag agggaaaaa 
      gtcgaagag attcagaaa agtcccgaa gctatgttt 
      ggggaactg gtggcagcg atgcttgcg aaaaagact 
      gaaagggac aaaaatata gccatgatc gaaatcatg 
      aaggtgttg accaaatga
    mRNA sequence
    • >TC009795 RA
      ccattcctt aatttaatg gaatctaat taacttggc 
      gtagtatcg acgaaattg caatcagta cgtgcggtg 
      aacgtcgaa agagcgcca tgagaccac aaatcctcc 
      caaaatcgc tctaaaaac ccgaatttt tccattttt 
      gctcgaatt tctcgtaat tactaaaac taacctcaa 
      aattagtca tggatcatg ccagattta ttagtgttg 
      tcaagcagt tcccccagg tgtgggacc gaaattgcg 
      ctactttca gggagcaga gcctgaaaa gtgaagctt 
      gggagagaa tcggcgaaa ttatgaatt gtccagctt 
      cgtcctgta aagacacct tcaagagtc ttcgggaga 
      agtatgtga gagaaaggg aaaaacagg ccatgtatg 
      cggacaagt acatgcctt ggcagcacc tggagagcc 
      tgtctttcc tggaccccc atattatcc tcaggaagc 
      agtcgctga actatgaca gtgacatgt cgaagctac 
      ccctcgacg agacaaaaa tgaacatga cggacgagc 
      catttttcg tccaactgg cggaaaatt acagcgatt 
      ttgacaaga ctttgatcg cgttggtgc gcaaggcga 
      gccccattt gggaccgca agagcaacg gttacccca 
      acaagcagg tgaagtacc agctgtggc ggggcatag 
      cccaggcca tgaacaagg atatcaacg tctgcatga 
      tgcgctgga aggggctca gggagaagt acatccgcc 
      agaaaaata agtaccaag aaggggagg gcaagtggg 
      agtttctgg atgacatgt cgttcctgg acaaggtga 
      tccaatacc ggcgcaaga actgcgaac cggatatga 
      gctttcgcc cccagagtg gccccgcag aggggtttc 
      cgtttcaca atgacatgt acgacgaca attcgatga 
      actcgtcgt caaacgatt ttataccaa ctgttaaaa 
      gtgaggatt ttggatgtg atatgcagg atagttcct 
      acgattccg ggacggcca gttccggaa ggaagaggt 
      cagcgagtg ataatagcg agtcgtcct acgaaaaga 
      gggaaaaag tcgaagaga ttcagaaaa gtcccgaag 
      ctatgtttg gggaactgg tggcagcga tgcttgcga 
      aaaagactg aaagggaca aaaatatag ccatgatcg 
      aaatcatga aggtgttga ccaaatgaa ggatctcag 
      agggtatat tttctcgga atagtgttt ggata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY