Status
Please wait ...
Gene information
General information
TC009793 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8197390..8202993
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009793
    iBeetle screen iB_04703

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    previtellogenic egg chamber partiallyshape irregular - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009793 RA
      MDHIFTINK KINKSVDTS KHLSFTKLE KSTLCSISS 
      SNVVAFSTE CNIEEAGSL FWNSNVYVC DLNSPWQVH 
      KILSNSSPI SVLQWDFTG DLLLVADEN GSIKIYKAK 
      DHILNDWTL VLQTVLEGE HILSAAFFH SGKKICLNS 
      EKKDSALYT EKFHHVKFA CSVKQFGGR PASGALLLT 
      TTGMLCSIL LPQSTSQVA MVMATESLA PTRIFIKAA 
      DICYGKNGH FLLAVSNGD LSMPIQCYK VSVRKAEDK 
      CVITSQSLL SFFLFEAPK EALMEQVSK EKNCHISHI 
      KWIMREDAD SVVVSATSD KISCLQVWE LREKALPVH 
      KSLGKSETP QFFNTVLWQ YQCHFQYNS RVTSLATSK 
      LSLLNNVSS GYVVATFAD NSIHCLYRD TLKHLVSTT 
      LTICPRYFD EPLARYPKT SIKISNIDM SWLGNILLV 
      IDSDDSLHM FKLPPQIDS STPLSVPYC TTILEYCMM 
      SGFDWLDLL LVLRPGMLD AICDRLTES FNRQPSAVQ 
      QFFYVQYLC MKISLCRLS VQGQVKAHD LAQFLMLHS 
      ISTAFKSLL RPSEMSSHD KSPADSLAN VIAEGQTDV 
      DKVLMHLEA KEFTVEPST LQSLQQLIQ WVADLALNL 
      LVKLPDSRP SMGKVYELL RDVKALNML REMLVLIRI 
      WGLLRPACL PVFIKSDGS LDVLALLFR LLSRLVQNV 
      NEPDDTLID ECCLLPSQV QIQLFQPSN NRAVLASPQ 
      LVQQILPLQ LEYNNDPEC LMSSTDSGA TQTVDSIRH 
      LYLGRTPRA IKQCVRCGG SAGTIPVTR TAAIRAWDQ 
      RWLRSCQCG GLWRMQVYT
    CDS sequence
    • >TC009793 RA
      atggaccat attttcacc attaataag aaaataaat 
      aaatcggtg gacaccagc aagcatcta tcattcacc 
      aaactcgag aagtcaacc ttatgctcg atttcaagc 
      tccaacgtt gttgctttc agcacggaa tgcaatatc 
      gaagaagcg ggttccctc ttttggaac tctaatgtc 
      tacgtctgc gatttaaac agtccttgg caggtccac 
      aaaatatta tcaaactct tcccccatt tcggtgctc 
      cagtgggac tttacgggc gaccttctg ctagtcgcg 
      gacgaaaac ggctctata aaaatttac aaagcaaaa 
      gaccacatc ctaaatgac tggaccctt gtcctccag 
      actgtccta gaaggggag cacattctg tcagcggcg 
      tttttccac tcgggcaag aaaatctgc ctcaactcc 
      gagaaaaaa gacagcgct ctgtacacg gaaaagttt 
      caccatgtc aagtttgcc tgctctgtt aaacagttt 
      ggggggcgc cctgccagt ggggccctg ctactcaca 
      acgacggga atgttgtgc tcgatttta ctcccccaa 
      agcacgagc caggtcgca atggtcatg gcgacagaa 
      agcctggcc cctactagg atttttatc aaagctgct 
      gatatttgc tatggcaaa aacggtcat tttttgctc 
      gctgtgagt aacggagac ttatcaatg cctattcag 
      tgttataaa gtctctgtc aggaaagct gaagataag 
      tgtgtcatc acaagccag tctttactc agttttttt 
      ctatttgag gcgcctaag gaagcatta atggaacaa 
      gtttccaaa gaaaaaaat tgccacata agtcatatc 
      aaatggata atgagagag gacgcagat tcggtggtt 
      gtttcagcc actagtgat aaaataagc tgtttgcaa 
      gtctgggaa ttgagagag aaggctttg ccggttcac 
      aaatcacta ggaaagtct gaaactccc cagtttttt 
      aacactgtc ttgtggcaa taccagtgc cacttccag 
      tacaacagc agggtcact tcactagct acaagtaaa 
      ctaagtcta ttaaataat gtttcaagt ggttatgtc 
      gtggccaca tttgccgac aattccatc cattgcctc 
      tacagagac accctaaaa catctggtc agcaccact 
      ttgactatt tgtccgcgt tattttgac gaacctctc 
      gccagatac cccaaaact agcatcaaa atctcaaac 
      attgacatg tcctggctc ggcaatatt ttactcgtg 
      attgattca gacgatagt ctgcatatg tttaagtta 
      ccgccccaa atcgacagc tcaacgcct ctaagtgta 
      ccttattgt accacgatt ttagagtat tgcatgatg 
      agcggtttt gattggtta gacttgttg ctagtgtta 
      aggcctgga atgttggac gcgatatgt gaccgactc 
      accgagagt tttaatcgc cagccctcg gcggtgcaa 
      cagtttttc tacgtgcag tatttgtgc atgaaaata 
      tcgctatgc aggttgagc gttcaaggc caagtgaag 
      gctcacgat ctggctcaa ttcctgatg cttcacagc 
      atatctaca gccttcaaa agccttcta agaccaagc 
      gagatgagc agtcatgac aaaagtcca gctgattcc 
      ttagcaaat gtaattgcg gaaggacaa accgacgtg 
      gataaggtt ttgatgcat ctcgaggcg aaagaattc 
      accgttgaa ccctcaact ctccaaagt ttgcaacaa 
      ctcatccaa tgggtggcc gacttggct cttaattta 
      ttggtcaaa ttgccagat agtcgtcct tccatggga 
      aaggtgtac gagttgttg cgcgatgtc aaagcgttg 
      aatatgttg agagaaatg ctggttttg atccgaatc 
      tgggggctt ttgagaccc gcttgtctc ccagttttt 
      atcaaatcg gatggaagt ttggacgtt ttggctctt 
      ctgttccga ctattaagc cgattggtc caaaacgtg 
      aacgaacca gatgatact ttaattgac gaatgttgc 
      ctccttcct agtcaagtc caaattcag cttttccag 
      ccgagcaat aatagggcc gttttggcg tccccacaa 
      ctcgtccaa caaattcta ccgttacag ttggagtat 
      aacaacgac cctgaatgt ctcatgtct tcgaccgac 
      tcgggggcg actcagaca gttgactca atcaggcat 
      ttgtatctg gggcgcacc cctagggct attaaacag 
      tgtgtaagg tgtgggggc tctgcaggg acgattccg 
      gtgacccgg actgcagca atacgcgcg tgggaccag 
      cggtggctc cgctcatgc cagtgtggt ggcctctgg 
      aggatgcaa gtttacaca tga
    mRNA sequence
    • >TC009793 RA
      ggtgtcaaa aatctgcca attcagtaa agttttaaa 
      aagatgctt cattagata aataccgaa aaatggacc 
      atattttca ccattaata agaaaataa ataaatcgg 
      tggacacca gcaagcatc tatcattca ccaaactcg 
      agaagtcaa ccttatgct cgatttcaa gctccaacg 
      ttgttgctt tcagcacgg aatgcaata tcgaagaag 
      cgggttccc tcttttgga actctaatg tctacgtct 
      gcgatttaa acagtcctt ggcaggtcc acaaaatat 
      tatcaaact cttccccca tttcggtgc tccagtggg 
      actttacgg gcgaccttc tgctagtcg cggacgaaa 
      acggctcta taaaaattt acaaagcaa aagaccaca 
      tcctaaatg actggaccc ttgtcctcc agactgtcc 
      tagaagggg agcacattc tgtcagcgg cgtttttcc 
      actcgggca agaaaatct gcctcaact ccgagaaaa 
      aagacagcg ctctgtaca cggaaaagt ttcaccatg 
      tcaagtttg cctgctctg ttaaacagt ttggggggc 
      gccctgcca gtggggccc tgctactca caacgacgg 
      gaatgttgt gctcgattt tactccccc aaagcacga 
      gccaggtcg caatggtca tggcgacag aaagcctgg 
      cccctacta ggattttta tcaaagctg ctgatattt 
      gctatggca aaaacggtc attttttgc tcgctgtga 
      gtaacggag acttatcaa tgcctattc agtgttata 
      aagtctctg tcaggaaag ctgaagata agtgtgtca 
      tcacaagcc agtctttac tcagttttt ttctatttg 
      aggcgccta aggaagcat taatggaac aagtttcca 
      aagaaaaaa attgccaca taagtcata tcaaatgga 
      taatgagag aggacgcag attcggtgg ttgtttcag 
      ccactagtg ataaaataa gctgtttgc aagtctggg 
      aattgagag agaaggctt tgccggttc acaaatcac 
      taggaaagt ctgaaactc cccagtttt ttaacactg 
      tcttgtggc aataccagt gccacttcc agtacaaca 
      gcagggtca cttcactag ctacaagta aactaagtc 
      tattaaata atgtttcaa gtggttatg tcgtggcca 
      catttgccg acaattcca tccattgcc tctacagag 
      acaccctaa aacatctgg tcagcacca ctttgacta 
      tttgtccgc gttattttg acgaacctc tcgccagat 
      accccaaaa ctagcatca aaatctcaa acattgaca 
      tgtcctggc tcggcaata ttttactcg tgattgatt 
      cagacgata gtctgcata tgtttaagt taccgcccc 
      aaatcgaca gctcaacgc ctctaagtg taccttatt 
      gtaccacga ttttagagt attgcatga tgagcggtt 
      ttgattggt tagacttgt tgctagtgt taaggcctg 
      gaatgttgg acgcgatat gtgaccgac tcaccgaga 
      gttttaatc gccagccct cggcggtgc aacagtttt 
      tctacgtgc agtatttgt gcatgaaaa tatcgctat 
      gcaggttga gcgttcaag gccaagtga aggctcacg 
      atctggctc aattcctga tgcttcaca gcatatcta 
      cagccttca aaagccttc taagaccaa gcgagatga 
      gcagtcatg acaaaagtc cagctgatt ccttagcaa 
      atgtaattg cggaaggac aaaccgacg tggataagg 
      ttttgatgc atctcgagg cgaaagaat tcaccgttg 
      aaccctcaa ctctccaaa gtttgcaac aactcatcc 
      aatgggtgg ccgacttgg ctcttaatt tattggtca 
      aattgccag atagtcgtc cttccatgg gaaaggtgt 
      acgagttgt tgcgcgatg tcaaagcgt tgaatatgt 
      tgagagaaa tgctggttt tgatccgaa tctgggggc 
      ttttgagac ccgcttgtc tcccagttt ttatcaaat 
      cggatggaa gtttggacg ttttggctc ttctgttcc 
      gactattaa gccgattgg tccaaaacg tgaacgaac 
      cagatgata ctttaattg acgaatgtt gcctccttc 
      ctagtcaag tccaaattc agcttttcc agccgagca 
      ataataggg ccgttttgg cgtccccac aactcgtcc 
      aacaaattc taccgttac agttggagt ataacaacg 
      accctgaat gtctcatgt cttcgaccg actcggggg 
      cgactcaga cagttgact caatcaggc atttgtatc 
      tggggcgca cccctaggg ctattaaac agtgtgtaa 
      ggtgtgggg gctctgcag ggacgattc cggtgaccc 
      ggactgcag caatacgcg cgtgggacc agcggtggc 
      tccgctcat gccagtgtg gtggcctct ggaggatgc 
      aagtttaca catgaattt aaggcatcg caaatgtgg 
      tactgatcg tgtaagagg ttgaaaatg atttaattt 
      tatatgaac gacttgtat aaataaaga tttaaggat 
      gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY