Status
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Gene information
General information
TC031200
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009790,TC009792 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8181260..8196904
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031200
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04702

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Adult morphology
    prothoracic odoriferous glands darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection


    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4

    egg chamber potentially number decreased - Number of animals showing the phenotype: 4

    egg chamber ap-orientation irregular - Number of animals showing the phenotype: 4

    Comment: egg chambers growing potentially in the tropharium
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031200 RA
      MGNCLSSPS NNDRIDRNR ERLGVNAIE DTSLPSNPM 
PTPPSEVVR PAVVNPEPE TTNAKIFVA LYDYDARTD 
EDLSFRKGE HLEILNDTQ GDWWLARSK RTRQEGYIP 
SNYVAKLKS IEAEPWYFR KIKRIEAEK KLLLPENEH 
GAFLIRDSE SRHNDYSLS VRDGDTVKH YRIRQLDEG 
GFFIARRTT FRTLQELVE HYSKDPDGL CVNLCKPCV 
QIEKPQPEG LSHRTRDQW EIERSSLKF VRKLGHGQF 
GEVWEGMWN NTTPVAIKT LKPGTMDPK DFLAEAQIM 
KKLRHPKLI QLYAVCTVE EPIYIITEL MRNGSLLEH 
LQGKGKSLK LQQLIDMAA QIAAGMAYL ESQNYIHRD 
LAARNVLVA DNNVVKIAD FGLARLIKE DEYEARVGA 
RFPIKWTAP EAANYNKFS IKSDVWSFG ILLTELVTY 
GRIPYPGMT NAEVLHQVE HGYRMPSPP GCPAPLYDI 
MLECWNRDP MKRPTFETL QWKLEDFFT MEGSEYKEA 
SAY
    CDS sequence
    • >TC031200 RA
      atggggaat tgcctcagc agtccgtcg aataacgac 
aggatagac aggaacagg gagaggctg ggcgtgaat 
gccatcgag gacaccagc ctgccctcc aaccccatg 
cccacgccc ccctcggag gtggtgcgg cccgcggtg 
gtcaacccg gaaccggag accaccaac gccaagatt 
ttcgtggcc ctttacgac tacgatgca aggactgat 
gaggatttg agtttcaga aaaggggaa catttggag 
atcttgaac gatacacag ggtgattgg tggctggcg 
aggagcaaa aggactaga caggaaggg tatatccct 
tcgaattat gtggccaag ttgaagagc atcgaagca 
gaaccatgg tatttccgg aaaatcaaa cgtatcgaa 
gcggaaaag aagctccta cttcccgag aacgaacac 
ggcgcattt ttgatccgc gattcggaa agccggcac 
aacgattac tcgttatca gtccgtgat ggagatacc 
gtcaaacat tacagaatc aggcaactg gatgaaggt 
ggtttcttc atcgcgcgg cgaacgacg tttagaacg 
cttcaagaa ctagtggaa cattacagt aaagaccca 
gacggtctg tgtgtcaat ctgtgcaaa ccatgcgtt 
cagatcgaa aaaccccag ccggagggc ttatcacac 
cgcacaagg gaccaatgg gagatcgag cgttcttcg 
ctgaaattc gtgcgaaaa ttaggccac ggccagttc 
ggcgaagtg tgggagggt atgtggaac aacaccaca 
cccgttgca ataaaaacc ctcaaacct ggaacgatg 
gacccgaaa gatttcctc gcagaagca caaatcatg 
aaaaaactt aggcatccg aaactgatc caactctat 
gccgtttgc acggttgag gaaccgatt tatatcatc 
acagaactg atgcgaaac ggctcgctg ttggaacat 
ctacagggt aaaggcaaa agtctgaaa ttgcagcaa 
ttgatcgat atggccgca caaatagcc gccggaatg 
gcgtattta gaatcgcag aattacata catagggat 
ctagcggcg aggaatgtt ctcgtggcc gataataat 
gtagtgaag atcgccgat ttcggactc gccaggctt 
ataaaggaa gatgaatat gaagcgcga gtcggtgca 
aggttcccc ataaaatgg acggctccc gaggcggcc 
aattacaat aaattttcg ataaaatcc gacgtatgg 
tcgtttgga attctcctg actgagctc gtcacatac 
ggcagaata ccatatcca ggtatgacc aatgccgag 
gtgttgcat caagtcgag cacgggtat cgcatgccg 
tcgcccccc ggctgtccc gcaccgctc tacgatatc 
atgttagaa tgttggaac cgtgaccca atgaaaagg 
ccgacgttt gagacgtta caatggaaa ttagaagac 
tttttcacc atggaaggt tcagaatac aaagaagcg 
tcggcttac tga
    mRNA sequence
    • >TC031200 RA
      ggcaggcga aagttacta tcgtccgtg ataggaaaa 
gtgcacttt tccagtgtt gttccgcga ttcccgtct 
gaaatcgtg atgggtttc gttatttag taacggtac 
gtcttctac gtgactgca taaatgtat ttaatttac 
ttacgaatt taatttacg aatcgaata acaacgaaa 
cacataaca atcaagtgt cccgtgttt gtgatggtg 
gttggattg ttgtgacaa gtgaagtgc ggattcaaa 
aatggggaa ttgcctcag cagtccgtc gaataacga 
caggataga caggaacag ggagaggct gggcgtgaa 
tgccatcga ggacaccag cctgccctc caaccccat 
gcccacgcc cccctcgga ggtggtgcg gcccgcggt 
ggtcaaccc ggaaccgga gaccaccaa cgccaagat 
tttcgtggc cctttacga ctacgatgc aaggactga 
tgaggattt gagtttcag aaaagggga acatttgga 
gatcttgaa cgatacaca gggtgattg gtggctggc 
gaggagcaa aaggactag acaggaagg gtatatccc 
ttcgaatta tgtggccaa gttgaagag catcgaagc 
agaaccatg gtatttccg gaaaatcaa acgtatcga 
agcggaaaa gaagctcct acttcccga gaacgaaca 
cggcgcatt tttgatccg cgattcgga aagccggca 
caacgatta ctcgttatc agtccgtga tggagatac 
cgtcaaaca ttacagaat caggcaact ggatgaagg 
tggtttctt catcgcgcg gcgaacgac gtttagaac 
gcttcaaga actagtgga acattacag taaagaccc 
agacggtct gtgtgtcaa tctgtgcaa accatgcgt 
tcagatcga aaaacccca gccggaggg cttatcaca 
ccgcacaag ggaccaatg ggagatcga gcgttcttc 
gctgaaatt cgtgcgaaa attaggcca cggccagtt 
cggcgaagt gtgggaggg tatgtggaa caacaccac 
acccgttgc aataaaaac cctcaaacc tggaacgat 
ggacccgaa agatttcct cgcagaagc acaaatcat 
gaaaaaact taggcatcc gaaactgat ccaactcta 
tgccgtttg cacggttga ggaaccgat ttatatcat 
cacagaact gatgcgaaa cggctcgct gttggaaca 
tctacaggg taaaggcaa aagtctgaa attgcagca 
attgatcga tatggccgc acaaatagc cgccggaat 
ggcgtattt agaatcgca gaattacat acataggga 
tctagcggc gaggaatgt tctcgtggc cgataataa 
tgtagtgaa gatcgccga tttcggact cgccaggct 
tataaagga agatgaata tgaagcgcg agtcggtgc 
aaggttccc cataaaatg gacggctcc cgaggcggc 
caattacaa taaattttc gataaaatc cgacgtatg 
gtcgtttgg aattctcct gactgagct cgtcacata 
cggcagaat accatatcc aggtatgac caatgccga 
ggtgttgca tcaagtcga gcacgggta tcgcatgcc 
gtcgccccc cggctgtcc cgcaccgct ctacgatat 
catgttaga atgttggaa ccgtgaccc aatgaaaag 
gccgacgtt tgagacgtt acaatggaa attagaaga 
ctttttcac catggaagg ttcagaata caaagaagc 
gtcggctta ctgaggcca cgcccaccc ccctgctga 
ctggctgcg taattcgat tgacttaca ctaccatta 
gtggggagg tggatatct atttctatg taacaatag 
atagatatg cccgtactc ttatttctt gtcgtgact 
atgcttgtg tttgaaata atggtttgt gaagtttta 
tcagatttt tacacgtcg actcggtat cacaaacca 
ctattttgc gattttaat tttaaactt tgcttcctt 
ttatacatc gtgaatggg gctcttcgc tctggctca 
tctattttt aaatattct ttatataat aatttattt 
taagatttt atgtaattt tatagtgaa attcgacaa 
cgttaaatt ttccccgat tgaaacttt taaacgcac 
aattcgtgt ctaccaata agaatgaaa cgatgtgta 
attgttgct tgtgcatat tacctaatt cccctaagt 
acttacgct tttgcattt attttttaa aaacgaata 
acattcctc tcaagtgac actatccga tatctctag 
cgttatttc ccagatgcc ctacagtta ttaaaaatc 
ggtactaat ttatgaaag gcgttgttt cttggttgt 
tttagtcac aacttatgt cttcattat tttaagatt 
gttattaaa ggcattgtt tatacagtt ataggccat 
tatgaaaat attttttgt attatacac ttagaatat 
acaataaaa gtcctttac aatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY