Status
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Gene information
General information
TC031200
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009790,TC009792
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8181260..8196904
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031200
    iBeetle screen iB_04702

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Adult morphology
    prothoracic odoriferous glands darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4

    egg chamber potentially number decreased - Number of animals showing the phenotype: 4

    egg chamber ap-orientation irregular - Number of animals showing the phenotype: 4

    Comment: egg chambers growing potentially in the tropharium
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031200 RA
      MGNCLSSPS NNDRIDRNR ERLGVNAIE DTSLPSNPM 
      PTPPSEVVR PAVVNPEPE TTNAKIFVA LYDYDARTD 
      EDLSFRKGE HLEILNDTQ GDWWLARSK RTRQEGYIP 
      SNYVAKLKS IEAEPWYFR KIKRIEAEK KLLLPENEH 
      GAFLIRDSE SRHNDYSLS VRDGDTVKH YRIRQLDEG 
      GFFIARRTT FRTLQELVE HYSKDPDGL CVNLCKPCV 
      QIEKPQPEG LSHRTRDQW EIERSSLKF VRKLGHGQF 
      GEVWEGMWN NTTPVAIKT LKPGTMDPK DFLAEAQIM 
      KKLRHPKLI QLYAVCTVE EPIYIITEL MRNGSLLEH 
      LQGKGKSLK LQQLIDMAA QIAAGMAYL ESQNYIHRD 
      LAARNVLVA DNNVVKIAD FGLARLIKE DEYEARVGA 
      RFPIKWTAP EAANYNKFS IKSDVWSFG ILLTELVTY 
      GRIPYPGMT NAEVLHQVE HGYRMPSPP GCPAPLYDI 
      MLECWNRDP MKRPTFETL QWKLEDFFT MEGSEYKEA 
      SAY
    CDS sequence
    • >TC031200 RA
      atggggaat tgcctcagc agtccgtcg aataacgac 
      aggatagac aggaacagg gagaggctg ggcgtgaat 
      gccatcgag gacaccagc ctgccctcc aaccccatg 
      cccacgccc ccctcggag gtggtgcgg cccgcggtg 
      gtcaacccg gaaccggag accaccaac gccaagatt 
      ttcgtggcc ctttacgac tacgatgca aggactgat 
      gaggatttg agtttcaga aaaggggaa catttggag 
      atcttgaac gatacacag ggtgattgg tggctggcg 
      aggagcaaa aggactaga caggaaggg tatatccct 
      tcgaattat gtggccaag ttgaagagc atcgaagca 
      gaaccatgg tatttccgg aaaatcaaa cgtatcgaa 
      gcggaaaag aagctccta cttcccgag aacgaacac 
      ggcgcattt ttgatccgc gattcggaa agccggcac 
      aacgattac tcgttatca gtccgtgat ggagatacc 
      gtcaaacat tacagaatc aggcaactg gatgaaggt 
      ggtttcttc atcgcgcgg cgaacgacg tttagaacg 
      cttcaagaa ctagtggaa cattacagt aaagaccca 
      gacggtctg tgtgtcaat ctgtgcaaa ccatgcgtt 
      cagatcgaa aaaccccag ccggagggc ttatcacac 
      cgcacaagg gaccaatgg gagatcgag cgttcttcg 
      ctgaaattc gtgcgaaaa ttaggccac ggccagttc 
      ggcgaagtg tgggagggt atgtggaac aacaccaca 
      cccgttgca ataaaaacc ctcaaacct ggaacgatg 
      gacccgaaa gatttcctc gcagaagca caaatcatg 
      aaaaaactt aggcatccg aaactgatc caactctat 
      gccgtttgc acggttgag gaaccgatt tatatcatc 
      acagaactg atgcgaaac ggctcgctg ttggaacat 
      ctacagggt aaaggcaaa agtctgaaa ttgcagcaa 
      ttgatcgat atggccgca caaatagcc gccggaatg 
      gcgtattta gaatcgcag aattacata catagggat 
      ctagcggcg aggaatgtt ctcgtggcc gataataat 
      gtagtgaag atcgccgat ttcggactc gccaggctt 
      ataaaggaa gatgaatat gaagcgcga gtcggtgca 
      aggttcccc ataaaatgg acggctccc gaggcggcc 
      aattacaat aaattttcg ataaaatcc gacgtatgg 
      tcgtttgga attctcctg actgagctc gtcacatac 
      ggcagaata ccatatcca ggtatgacc aatgccgag 
      gtgttgcat caagtcgag cacgggtat cgcatgccg 
      tcgcccccc ggctgtccc gcaccgctc tacgatatc 
      atgttagaa tgttggaac cgtgaccca atgaaaagg 
      ccgacgttt gagacgtta caatggaaa ttagaagac 
      tttttcacc atggaaggt tcagaatac aaagaagcg 
      tcggcttac tga
    mRNA sequence
    • >TC031200 RA
      ggcaggcga aagttacta tcgtccgtg ataggaaaa 
      gtgcacttt tccagtgtt gttccgcga ttcccgtct 
      gaaatcgtg atgggtttc gttatttag taacggtac 
      gtcttctac gtgactgca taaatgtat ttaatttac 
      ttacgaatt taatttacg aatcgaata acaacgaaa 
      cacataaca atcaagtgt cccgtgttt gtgatggtg 
      gttggattg ttgtgacaa gtgaagtgc ggattcaaa 
      aatggggaa ttgcctcag cagtccgtc gaataacga 
      caggataga caggaacag ggagaggct gggcgtgaa 
      tgccatcga ggacaccag cctgccctc caaccccat 
      gcccacgcc cccctcgga ggtggtgcg gcccgcggt 
      ggtcaaccc ggaaccgga gaccaccaa cgccaagat 
      tttcgtggc cctttacga ctacgatgc aaggactga 
      tgaggattt gagtttcag aaaagggga acatttgga 
      gatcttgaa cgatacaca gggtgattg gtggctggc 
      gaggagcaa aaggactag acaggaagg gtatatccc 
      ttcgaatta tgtggccaa gttgaagag catcgaagc 
      agaaccatg gtatttccg gaaaatcaa acgtatcga 
      agcggaaaa gaagctcct acttcccga gaacgaaca 
      cggcgcatt tttgatccg cgattcgga aagccggca 
      caacgatta ctcgttatc agtccgtga tggagatac 
      cgtcaaaca ttacagaat caggcaact ggatgaagg 
      tggtttctt catcgcgcg gcgaacgac gtttagaac 
      gcttcaaga actagtgga acattacag taaagaccc 
      agacggtct gtgtgtcaa tctgtgcaa accatgcgt 
      tcagatcga aaaacccca gccggaggg cttatcaca 
      ccgcacaag ggaccaatg ggagatcga gcgttcttc 
      gctgaaatt cgtgcgaaa attaggcca cggccagtt 
      cggcgaagt gtgggaggg tatgtggaa caacaccac 
      acccgttgc aataaaaac cctcaaacc tggaacgat 
      ggacccgaa agatttcct cgcagaagc acaaatcat 
      gaaaaaact taggcatcc gaaactgat ccaactcta 
      tgccgtttg cacggttga ggaaccgat ttatatcat 
      cacagaact gatgcgaaa cggctcgct gttggaaca 
      tctacaggg taaaggcaa aagtctgaa attgcagca 
      attgatcga tatggccgc acaaatagc cgccggaat 
      ggcgtattt agaatcgca gaattacat acataggga 
      tctagcggc gaggaatgt tctcgtggc cgataataa 
      tgtagtgaa gatcgccga tttcggact cgccaggct 
      tataaagga agatgaata tgaagcgcg agtcggtgc 
      aaggttccc cataaaatg gacggctcc cgaggcggc 
      caattacaa taaattttc gataaaatc cgacgtatg 
      gtcgtttgg aattctcct gactgagct cgtcacata 
      cggcagaat accatatcc aggtatgac caatgccga 
      ggtgttgca tcaagtcga gcacgggta tcgcatgcc 
      gtcgccccc cggctgtcc cgcaccgct ctacgatat 
      catgttaga atgttggaa ccgtgaccc aatgaaaag 
      gccgacgtt tgagacgtt acaatggaa attagaaga 
      ctttttcac catggaagg ttcagaata caaagaagc 
      gtcggctta ctgaggcca cgcccaccc ccctgctga 
      ctggctgcg taattcgat tgacttaca ctaccatta 
      gtggggagg tggatatct atttctatg taacaatag 
      atagatatg cccgtactc ttatttctt gtcgtgact 
      atgcttgtg tttgaaata atggtttgt gaagtttta 
      tcagatttt tacacgtcg actcggtat cacaaacca 
      ctattttgc gattttaat tttaaactt tgcttcctt 
      ttatacatc gtgaatggg gctcttcgc tctggctca 
      tctattttt aaatattct ttatataat aatttattt 
      taagatttt atgtaattt tatagtgaa attcgacaa 
      cgttaaatt ttccccgat tgaaacttt taaacgcac 
      aattcgtgt ctaccaata agaatgaaa cgatgtgta 
      attgttgct tgtgcatat tacctaatt cccctaagt 
      acttacgct tttgcattt attttttaa aaacgaata 
      acattcctc tcaagtgac actatccga tatctctag 
      cgttatttc ccagatgcc ctacagtta ttaaaaatc 
      ggtactaat ttatgaaag gcgttgttt cttggttgt 
      tttagtcac aacttatgt cttcattat tttaagatt 
      gttattaaa ggcattgtt tatacagtt ataggccat 
      tatgaaaat attttttgt attatacac ttagaatat 
      acaataaaa gtcctttac aatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY