Status
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Gene information
General information
TC009785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8122792..8124756
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009785
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04699

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009785 RA
      MEAQSQIVE SFKNQTIFL TGGSGFVGK 
      VFLAKLLTV CPEVNTIYL LVRPKRNKT 
      PSERLRDIF SFSCFEPLK SKWPNFQEK 
      IKLIIGDCE KIGLDIAAQ DREILRREV 
      TIFLHAAAN VKFDQSLKL ATYANVRAM 
      REVLALVKE MTRLKILVR YPCFLSAHI 
      TRILCRCYS NMSG
    • >TC009785 RB
      MEAQSQIVE SFKNQTIFL TGGSGFVGK VFLAKLLTV 
      CPEVNTIYL LVRPKRNKT PSERLRDIF SFSCFEPLK 
      SKWPNFQEK IKLIIGDCE KIGLDIAAQ DREILRREV 
      TIFLHAAAN VKFDQSLKL ATYANVRAM REVLALVKE 
      MTRLKAFTY VSTVYSNCP HSHIGEDFY ESGMKAESL 
      LTLVESVDE SVLDAMTPS LLKTWPNTY VFTKCISED 
      LLKREAGDL PVAVVRPCI IMPTAREPV PAWSDNFYG 
      IIGICVGVL AGVIKVMPG KPANPLHVI PCDYVVNLI 
      MASVWDLLQ PKSTVKENK IAIYNHVPP PENTCTLGH 
      LVATINKIK WVYPFSDMA YWFPLFTMV TCRYWYAIR 
      AFFQHTLLA YFADVILTC LGRKPIAVQ QTRKISKLV 
      ILLSYFTLN TWTFDFHNV EALWDRLSE KDQKLFRFE 
      LKSLNWDDF WKLSIKHGR QYLLKEKLE DLPYGRKKV 
      RVLAVIHYA TCALLLYLS YKILQFVFG FMF
    CDS sequence
    • >TC009785 RA
      atggaggcc caaagccag attgttgaa tcgtttaag 
      aatcaaacg attttttta actgggggc tcaggcttc 
      gtcgggaaa gtgttccta gcgaaactg ttaaccgtt 
      tgtcccgaa gtgaataca atttacctc cttgtgcgc 
      cccaaacga aacaaaacg ccgagtgaa cgacttcgg 
      gacattttt agtttttcg tgcttcgaa cctctcaaa 
      tcaaaatgg ccaaacttc caagaaaaa atcaaactg 
      ataattgga gactgcgaa aaaatcggc ctggacatt 
      gcggcacaa gaccgggaa attttgaga cgtgaagtg 
      acgatattt ttacacgcg gcggcgaat gttaagttt 
      gaccagagt ttgaaactc gccacttat gctaacgtt 
      cgagccatg agggaggtt ttggccttg gtcaaggaa 
      atgactcga ttgaagata ctggtacgc tatccgtgc 
      tttctttca gcacacatt actcgcata ctttgccga 
      tgttattct aacatgtct gggtag
    • >TC009785 RB
      atggaggcc caaagccag attgttgaa tcgtttaag 
      aatcaaacg attttttta actgggggc tcaggcttc 
      gtcgggaaa gtgttccta gcgaaactg ttaaccgtt 
      tgtcccgaa gtgaataca atttacctc cttgtgcgc 
      cccaaacga aacaaaacg ccgagtgaa cgacttcgg 
      gacattttt agtttttcg tgcttcgaa cctctcaaa 
      tcaaaatgg ccaaacttc caagaaaaa atcaaactg 
      ataattgga gactgcgaa aaaatcggc ctggacatt 
      gcggcacaa gaccgggaa attttgaga cgtgaagtg 
      acgatattt ttacacgcg gcggcgaat gttaagttt 
      gaccagagt ttgaaactc gccacttat gctaacgtt 
      cgagccatg agggaggtt ttggccttg gtcaaggaa 
      atgactcga ttgaaggct tttacttat gtgtccacg 
      gtgtattca aactgtcct cattctcac attggggag 
      gatttctac gagtctgga atgaaagca gaaagttta 
      ttaacgctt gttgaaagt gtcgacgag agtgtttta 
      gatgccatg accccgagt ttgttgaaa acgtggccc 
      aacacttac gttttcacc aagtgtatt agcgaggat 
      ttgcttaag agagaagcg ggggattta ccggtggca 
      gttgtccga ccttgcata attatgcca actgcgaga 
      gaaccagtt ccagcgtgg agtgataat ttttacggc 
      attataggc atctgtgtt ggggttctg gcaggggtg 
      ataaaggtc atgcctggt aaaccagct aatcccctg 
      catgttata ccctgtgac tatgtggtt aatttaatt 
      atggctagt gtttgggat ttgttgcaa ccaaaatcc 
      actgtgaag gaaaataaa attgccatt tataatcac 
      gttcctcca cccgaaaat acttgtact cttggtcac 
      ttagtggct accattaac aaaataaaa tgggtctac 
      ccctttagc gacatggct tactggttt cccttattt 
      acaatggtc acttgtaga tactggtac gctatccgt 
      gctttcttt cagcacaca ttactcgca tactttgcc 
      gatgttatt ctaacatgt ctgggtaga aagccaata 
      gcggtgcaa caaacacgc aaaattagt aaactggta 
      atcctcttg tcttatttt actttaaac acatggacg 
      tttgatttt cataatgtt gaagcttta tgggatagg 
      ttgagtgaa aaagatcag aagttgttc agatttgag 
      cttaaatct ttgaattgg gatgatttt tggaaacta 
      tcaatcaag catggacgg cagtatttg ttaaaggaa 
      aagttggaa gatttgcct tatgggagg aaaaaagtt 
      agagtactc gccgttatc cactacgct acttgtgct 
      ttgttgttg tatttaagt tacaagatt ttacaattt 
      gtattcggc tttatgttt taa
    mRNA sequence
    • >TC009785 RA
      gcatgctta attatacaa gagttaaaa acaccacct 
      tcaagtgaa aaacacttt cagctcgac ttttccaca 
      agcagcatg gaggcccaa agccagatt gttgaatcg 
      tttaagaat caaacgatt tttttaact gggggctca 
      ggcttcgtc gggaaagtg ttcctagcg aaactgtta 
      accgtttgt cccgaagtg aatacaatt tacctcctt 
      gtgcgcccc aaacgaaac aaaacgccg agtgaacga 
      cttcgggac atttttagt ttttcgtgc ttcgaacct 
      ctcaaatca aaatggcca aacttccaa gaaaaaatc 
      aaactgata attggagac tgcgaaaaa atcggcctg 
      gacattgcg gcacaagac cgggaaatt ttgagacgt 
      gaagtgacg atattttta cacgcggcg gcgaatgtt 
      aagtttgac cagagtttg aaactcgcc acttatgct 
      aacgttcga gccatgagg gaggttttg gccttggtc 
      aaggaaatg actcgattg aagatactg gtacgctat 
      ccgtgcttt ctttcagca cacattact cgcatactt 
      tgccgatgt tattctaac atgtctggg tagaaagcc 
      aatgttggt actaacata ttacgttaa aaatattaa 
      atgtttttt tacagagcg gtgcaacaa acacgcaaa 
      attagtaaa ctggtaatc ctcttgtct tattttact 
      ttaaacaca tggacgttt gattttcat aatgttgaa 
      gctttatgg gataggttg agtgaaaaa gatcagaag 
      ttgttcaga tttgagctt aaatctttg aattgggat 
      gatttttgg aaactatca atcaagcat ggacggcag 
      tatttgtta aaggaaaag ttggaagat ttgccttat 
      gggaggaaa aaagttaga gtactcgcc gttatccac 
      tacgctact tgtgctttg ttgttgtat ttaagttac 
      aagatttta caatttgta ttcggcttt atgttttaa 
      tttaatctc taatttaag tggaatgtt ttcagtaaa 
      taaataatt caaattaag
    • >TC009785 RB
      gcatgctta attatacaa gagttaaaa acaccacct 
      tcaagtgaa aaacacttt cagctcgac ttttccaca 
      agcagcatg gaggcccaa agccagatt gttgaatcg 
      tttaagaat caaacgatt tttttaact gggggctca 
      ggcttcgtc gggaaagtg ttcctagcg aaactgtta 
      accgtttgt cccgaagtg aatacaatt tacctcctt 
      gtgcgcccc aaacgaaac aaaacgccg agtgaacga 
      cttcgggac atttttagt ttttcgtgc ttcgaacct 
      ctcaaatca aaatggcca aacttccaa gaaaaaatc 
      aaactgata attggagac tgcgaaaaa atcggcctg 
      gacattgcg gcacaagac cgggaaatt ttgagacgt 
      gaagtgacg atattttta cacgcggcg gcgaatgtt 
      aagtttgac cagagtttg aaactcgcc acttatgct 
      aacgttcga gccatgagg gaggttttg gccttggtc 
      aaggaaatg actcgattg aaggctttt acttatgtg 
      tccacggtg tattcaaac tgtcctcat tctcacatt 
      ggggaggat ttctacgag tctggaatg aaagcagaa 
      agtttatta acgcttgtt gaaagtgtc gacgagagt 
      gttttagat gccatgacc ccgagtttg ttgaaaacg 
      tggcccaac acttacgtt ttcaccaag tgtattagc 
      gaggatttg cttaagaga gaagcgggg gatttaccg 
      gtggcagtt gtccgacct tgcataatt atgccaact 
      gcgagagaa ccagttcca gcgtggagt gataatttt 
      tacggcatt ataggcatc tgtgttggg gttctggca 
      ggggtgata aaggtcatg cctggtaaa ccagctaat 
      cccctgcat gttataccc tgtgactat gtggttaat 
      ttaattatg gctagtgtt tgggatttg ttgcaacca 
      aaatccact gtgaaggaa aataaaatt gccatttat 
      aatcacgtt cctccaccc gaaaatact tgtactctt 
      ggtcactta gtggctacc attaacaaa ataaaatgg 
      gtctacccc tttagcgac atggcttac tggtttccc 
      ttatttaca atggtcact tgtagatac tggtacgct 
      atccgtgct ttctttcag cacacatta ctcgcatac 
      tttgccgat gttattcta acatgtctg ggtagaaag 
      ccaatagcg gtgcaacaa acacgcaaa attagtaaa 
      ctggtaatc ctcttgtct tattttact ttaaacaca 
      tggacgttt gattttcat aatgttgaa gctttatgg 
      gataggttg agtgaaaaa gatcagaag ttgttcaga 
      tttgagctt aaatctttg aattgggat gatttttgg 
      aaactatca atcaagcat ggacggcag tatttgtta 
      aaggaaaag ttggaagat ttgccttat gggaggaaa 
      aaagttaga gtactcgcc gttatccac tacgctact 
      tgtgctttg ttgttgtat ttaagttac aagatttta 
      caatttgta ttcggcttt atgttttaa tttaatctc 
      taatttaag tggaatgtt ttcagtaaa taaataatt 
      caaattaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY