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Gene information
General information
TC009782 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8087292..8107348
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009782
    iBeetle screen iB_04697

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partiallythinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature sometimes orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    Pupal morphology
    elytra size shortened - Number of animals showing the phenotype: 8

    female genital lobes shortened - Number of animals showing the phenotype: 8

    abdomen gin-traps not present - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009782 RA
      MDVDEAVVD LSISSNRSA SPAPQQQPQ QQQQQQPMG 
      IVSTNSAQS MVYSRRVLR PRTEPRSYV ESPDVLING 
      TLDKPRTNG NADYSSDSS EGEMPPLAP IKELSPSEL 
      KQRDRGLRR LREDLRAEE MKLVLLKKL KQSQQLKEN 
      VAVLPPSIP QSALPPTGL PSGLSITPT TVKVPSNKN 
      QPPQSLHSR HPNSHKTGQ GSVPSLLRG QPPPARSSI 
      HAPPLSGSP LSSRGANLS VPQPPQRSS LGRPPSGFP 
      PGVKDLSPS VTITPAPPQ PSKDRSRDD GQTPAQRQA 
      AAKLALRKQ LEKTLLQIP PPKPPPPEM HFIPNPSNT 
      EFIYLVGLE HVVDFLTKE TRMPLPPMP FSCSQCNSD 
      FTPVWKWET KGKNRGIKV ICEQCVTNN VKKALKAEH 
      TNRLKTAFV KALQQEQEI EQRLAQNGV FTSSSPAPS 
      PVTPEAVPK SATPTPTPR HAATPPAPS AQQTPPPSR 
      SSHSSSDKF SQNAAMQAL QQQLLRSLT AGAPPAHML 
      PFSPLFYPY QMAIAQAAA SGKGGTPNM AELQRAAEL 
      QRQYLLDMI PPGPGQRHN WKT
    • >TC009782 RB
      MDVDEAVVD LSISSNRSA SPAPQQQPQ QQQQQQPMG 
      IVSTNSAQS MVYSRRVLR PRTEPRSYV ESPDVLING 
      TLDKPRTNG NADYSSDSS EGEMPPLAP IKELSPSEL 
      KQRDRGLRR LREDLRAEE MKLVLLKKL KQSQQLKEN 
      VAVLPPSIP QSALPPTGL PSGLSITPT TVKVPSNKN 
      QPPQSLHSR HPNSHKTGQ GSVPSLLRG DRSRDDGQT 
      PAQRQAAAK LALRKQLEK TLLQIPPPK PPPPEMHFI 
      PNPSNTEFI YLVGLEHVV DFLTKETRM PLPPMPFSC 
      SQCNSDFTP VWKWETKGK NRGIKVICE QCVTNNVKK 
      ALKAEHTNR LKTAFVKAL QQEQEIEQR LAQNGVFTS 
      SSPAPSPVT PEAVPKSAT PTPTPRHAA TPPAPSAQQ 
      TPPPSRSSH SSSDKFSQN AAMQALQQQ LLRSLTAGA 
      PPAHMLPFS PLFYPYQMA IAQAAASGK GGTPNMAEL 
      QRAAELQRQ YLLDMIPPG PGQRHNWKT
    CDS sequence
    • >TC009782 RA
      atggacgtt gatgaagct gtcgtcgat ctgagtatt 
      agttcaaac cgatcagct tcaccagcc ccacagcag 
      cagccgcag caacagcag caacaacaa ccaatgggc 
      attgtgtcg acaaatagc gcccagtcg atggtctat 
      tcgagacga gtgcttcgc ccccgcact gaaccaaga 
      agttacgtc gaaagcccc gacgtgctc attaatggc 
      actctagat aaaccgcgg acgaacgga aacgcggat 
      tatagcagc gattcgagc gaaggggaa atgcctccg 
      ttagcgccc atcaaggag ctcagcccc tcggagctg 
      aagcagaga gacagaggc ctgagacga ctccgcgaa 
      gatctccgc gccgaagag atgaagctg gtgctgttg 
      aaaaaattg aaacaatcg caacagctg aaggagaac 
      gtggcggtg ttgcctccg agcatacct cagtcggcg 
      ttacctccg accggtctt ccaagcggt ctcagcatc 
      acaccgacc actgtcaaa gtaccatcg aacaagaac 
      caaccaccg caatcgctt cattcccga catccgaat 
      tcgcacaag actggccaa ggaagtgtt ccctcactt 
      ctgcgtgga caaccgccc ccagctcgt tcaagtatc 
      cacgcgccg cctctgtcc ggctcgcct ctgagtagc 
      cgaggcgcg aatctatcc gtacctcaa ccgccacaa 
      aggagttct ttaggtcga cctccttca ggtttccct 
      ccaggcgtt aaggatctc tcgccttcc gttacaatc 
      acacccgca ccaccccaa ccgtctaag gaccgatcg 
      agggacgat ggacagaca ccggcgcaa agacaagcg 
      gcggcgaag ttggcccta cgaaaacaa ttggaaaag 
      acgctacta cagatccct ccgcccaag ccgcctccg 
      cctgaaatg catttcatc cctaatcct agcaacacc 
      gaattcata tatctggta ggcttagaa cacgtcgtt 
      gatttcctg acgaaggag acgcgaatg ccgctgccg 
      ccaatgccg ttttcgtgt agtcaatgc aattctgat 
      tttaccccg gtttggaag tgggaaact aaaggcaaa 
      aatagaggg attaaagtc atttgtgag caatgtgta 
      accaataac gtgaagaaa gcgctcaaa gccgaacat 
      acgaaccgg ctcaagaca gcgtttgtg aaagctctt 
      caacaggag caggaaatc gagcagagg cttgctcaa 
      aatggggtt ttcacttcg agcagtccg gcaccgtcg 
      ccagtaact ccagaagcc gttcctaaa tcggcgacc 
      ccgactcct acgcctcga catgcggcc actccccca 
      gccccttct gctcaacaa acgcctcca ccctccagg 
      agttcacac tcgtcgtcg gataagttc tcgcagaat 
      gcggcgatg caagcgctg caacaacag ctgctcaga 
      agcttgacg gcgggagct ccgccggcg cacatgctg 
      ccgttctcg ccgctgttc tacccttac cagatggcc 
      atagcgcag gcggccgcc agcggcaaa gggggcaca 
      ccgaacatg gctgagctg cagcgggcc gccgagctg 
      caacgacag tacctcctc gacatgatc cctccaggt 
      ccgggtcag cgtcacaat tggaaaaca tga
    • >TC009782 RB
      atggacgtt gatgaagct gtcgtcgat ctgagtatt 
      agttcaaac cgatcagct tcaccagcc ccacagcag 
      cagccgcag caacagcag caacaacaa ccaatgggc 
      attgtgtcg acaaatagc gcccagtcg atggtctat 
      tcgagacga gtgcttcgc ccccgcact gaaccaaga 
      agttacgtc gaaagcccc gacgtgctc attaatggc 
      actctagat aaaccgcgg acgaacgga aacgcggat 
      tatagcagc gattcgagc gaaggggaa atgcctccg 
      ttagcgccc atcaaggag ctcagcccc tcggagctg 
      aagcagaga gacagaggc ctgagacga ctccgcgaa 
      gatctccgc gccgaagag atgaagctg gtgctgttg 
      aaaaaattg aaacaatcg caacagctg aaggagaac 
      gtggcggtg ttgcctccg agcatacct cagtcggcg 
      ttacctccg accggtctt ccaagcggt ctcagcatc 
      acaccgacc actgtcaaa gtaccatcg aacaagaac 
      caaccaccg caatcgctt cattcccga catccgaat 
      tcgcacaag actggccaa ggaagtgtt ccctcactt 
      ctgcgtgga gaccgatcg agggacgat ggacagaca 
      ccggcgcaa agacaagcg gcggcgaag ttggcccta 
      cgaaaacaa ttggaaaag acgctacta cagatccct 
      ccgcccaag ccgcctccg cctgaaatg catttcatc 
      cctaatcct agcaacacc gaattcata tatctggta 
      ggcttagaa cacgtcgtt gatttcctg acgaaggag 
      acgcgaatg ccgctgccg ccaatgccg ttttcgtgt 
      agtcaatgc aattctgat tttaccccg gtttggaag 
      tgggaaact aaaggcaaa aatagaggg attaaagtc 
      atttgtgag caatgtgta accaataac gtgaagaaa 
      gcgctcaaa gccgaacat acgaaccgg ctcaagaca 
      gcgtttgtg aaagctctt caacaggag caggaaatc 
      gagcagagg cttgctcaa aatggggtt ttcacttcg 
      agcagtccg gcaccgtcg ccagtaact ccagaagcc 
      gttcctaaa tcggcgacc ccgactcct acgcctcga 
      catgcggcc actccccca gccccttct gctcaacaa 
      acgcctcca ccctccagg agttcacac tcgtcgtcg 
      gataagttc tcgcagaat gcggcgatg caagcgctg 
      caacaacag ctgctcaga agcttgacg gcgggagct 
      ccgccggcg cacatgctg ccgttctcg ccgctgttc 
      tacccttac cagatggcc atagcgcag gcggccgcc 
      agcggcaaa gggggcaca ccgaacatg gctgagctg 
      cagcgggcc gccgagctg caacgacag tacctcctc 
      gacatgatc cctccaggt ccgggtcag cgtcacaat 
      tggaaaaca tga
    mRNA sequence
    • >TC009782 RA
      gtgcgacaa agacggact cggtgaggt gcgtcgtgg 
      cttgcgccg cgcgctgga tgaataaag gggcggccg 
      gcgcgatct gcgacgtat gtcgtgaaa ggtaggagt 
      gacgtagta aaagttcga ctgttcacg atacgtctg 
      ttttatcaa cgcggtagt gtttgtaga cacaacaca 
      aggccacgc catattgaa ttgaatcag tcgtctgtt 
      aatttcgga gcggagtga agtgcggcc gattttcgt 
      gttttgtgt gctgggggg ccgatgagg ccgccgggg 
      acgtgccag tgccgggcc gaagagtgc aagtgataa 
      gataatcga tttggcgac gcgatacat tttcggtgc 
      actgataat tgagccgag gtgcgctgg gatcgcgcc 
      gtgtcatgt tctgagagg ttagggggc tcatggaga 
      agatggacg ttgatgaag ctgtcgtcg atctgagta 
      ttagttcaa accgatcag cttcaccag ccccacagc 
      agcagccgc agcaacagc agcaacaac aaccaatgg 
      gcattgtgt cgacaaata gcgcccagt cgatggtct 
      attcgagac gagtgcttc gcccccgca ctgaaccaa 
      gaagttacg tcgaaagcc ccgacgtgc tcattaatg 
      gcactctag ataaaccgc ggacgaacg gaaacgcgg 
      attatagca gcgattcga gcgaagggg aaatgcctc 
      cgttagcgc ccatcaagg agctcagcc cctcggagc 
      tgaagcaga gagacagag gcctgagac gactccgcg 
      aagatctcc gcgccgaag agatgaagc tggtgctgt 
      tgaaaaaat tgaaacaat cgcaacagc tgaaggaga 
      acgtggcgg tgttgcctc cgagcatac ctcagtcgg 
      cgttacctc cgaccggtc ttccaagcg gtctcagca 
      tcacaccga ccactgtca aagtaccat cgaacaaga 
      accaaccac cgcaatcgc ttcattccc gacatccga 
      attcgcaca agactggcc aaggaagtg ttccctcac 
      ttctgcgtg gacaaccgc ccccagctc gttcaagta 
      tccacgcgc cgcctctgt ccggctcgc ctctgagta 
      gccgaggcg cgaatctat ccgtacctc aaccgccac 
      aaaggagtt ctttaggtc gacctcctt caggtttcc 
      ctccaggcg ttaaggatc tctcgcctt ccgttacaa 
      tcacacccg caccacccc aaccgtcta aggaccgat 
      cgagggacg atggacaga caccggcgc aaagacaag 
      cggcggcga agttggccc tacgaaaac aattggaaa 
      agacgctac tacagatcc ctccgccca agccgcctc 
      cgcctgaaa tgcatttca tccctaatc ctagcaaca 
      ccgaattca tatatctgg taggcttag aacacgtcg 
      ttgatttcc tgacgaagg agacgcgaa tgccgctgc 
      cgccaatgc cgttttcgt gtagtcaat gcaattctg 
      attttaccc cggtttgga agtgggaaa ctaaaggca 
      aaaatagag ggattaaag tcatttgtg agcaatgtg 
      taaccaata acgtgaaga aagcgctca aagccgaac 
      atacgaacc ggctcaaga cagcgtttg tgaaagctc 
      ttcaacagg agcaggaaa tcgagcaga ggcttgctc 
      aaaatgggg ttttcactt cgagcagtc cggcaccgt 
      cgccagtaa ctccagaag ccgttccta aatcggcga 
      ccccgactc ctacgcctc gacatgcgg ccactcccc 
      cagcccctt ctgctcaac aaacgcctc caccctcca 
      ggagttcac actcgtcgt cggataagt tctcgcaga 
      atgcggcga tgcaagcgc tgcaacaac agctgctca 
      gaagcttga cggcgggag ctccgccgg cgcacatgc 
      tgccgttct cgccgctgt tctaccctt accagatgg 
      ccatagcgc aggcggccg ccagcggca aagggggca 
      caccgaaca tggctgagc tgcagcggg ccgccgagc 
      tgcaacgac agtacctcc tcgacatga tccctccag 
      gtccgggtc agcgtcaca attggaaaa catgatgtg 
      tcagtgata agatttaga taatgacgt gctaaaaaa 
      gtgatcgag actatgtat aagaagaag ccatcgagg 
      ctgtcatcc gcgaggatc taaatgtcg atgtgccct 
      tttgttcca gaagtaaaa atcgctatt tattattta 
      acagtttga gtggaaatt tagtcaatt atcgcaaga 
      ctgattcta gtacctatt gtattaccg acactggag 
      gttattgaa aaaatagaa aaattaact cactaaacg 
      aacggagat ctcaggatt gacatactt acactaagt 
      ttaaacgat ttatttcct taacgttaa gttttcgga 
      tctttttac ttctggaat gtgttttat ttattacgt 
      gatgttctc gagacagtg caggacatg taaataagc 
      tttggcgaa attttttag atgtagaaa ttacggttg 
      acaatgcta aagcagaga cgtgtaagg ttgtttgta 
      atcgtgtga ttgaattac ttttcaacg gatttaagg 
      cattcaacg tttacaatg cacgattag ttgaatttt 
      tttcgaggc atttatttt taaagtgaa tgcttgaat 
      gcggaatcc ttacattat aacaagttt gtaaatagg 
      tgtttttac aattgtata atagttaat caataggaa 
      tattgatgc atattatgc tcattaatc ttgatgttg 
      acatttatt atgaggtgt tttctatta taacaaaaa 
      cagtgtttt ctaagacaa ctagctgaa aaaatgtac 
      tttctgtaa aaaaagaaa atatttatt tcagatgta 
      atcataaaa gtggtattt tcgaggcac cttaaatta 
      ttcaatgca gctaaagga atggaaact gaggggtga 
      agagtagtt tttttttat tggatttca ctgtactgt 
      gttcatttt taatactgt attttttcc attagaatc 
      gtgtacatt ttagcgata tgagggtgc taacagaaa 
      tttcgattt ctgatgata atctaaatg aatttttgt 
      ttgtattaa tgggggagt gtttggatt aacacaatt 
      ttgtttttg cacattttt cgtcacaaa actatgaag 
      ctcaatgaa attattttc taatctaaa aaatatcag 
      taagactta tttagagca caattatgc cagtaaaaa 
      tatgtaaat gcatttcaa atatctgca gtgtgaaaa 
      atcgtctac ataataaaa tcaacttct cagtcttac 
      ttgtattac tagtgttat tattgtagg tagtgcttg 
      caatttttc catctaaac tatagtcaa tctttgtcg 
      gtaataaat cggctgtgg aattatttt ttgattttc 
      attttcttt ttcagtcat acagtatca tacacaagc 
      tgaacaggt ctttaggga gctttataa actacagtt 
      tgtaaataa cagtttcta aaaactaaa tgcctcttc 
      agtacttac gttaatatg ttgcctcaa agttttcaa 
      ctattctat tttgtaagt gtataatta tttcaacat 
      gtttttaaa taaattgac tcataagtt
    • >TC009782 RB
      gtgcgacaa agacggact cggtgaggt gcgtcgtgg 
      cttgcgccg cgcgctgga tgaataaag gggcggccg 
      gcgcgatct gcgacgtat gtcgtgaaa ggtaggagt 
      gacgtagta aaagttcga ctgttcacg atacgtctg 
      ttttatcaa cgcggtagt gtttgtaga cacaacaca 
      aggccacgc catattgaa ttgaatcag tcgtctgtt 
      aatttcgga gcggagtga agtgcggcc gattttcgt 
      gttttgtgt gctgggggg ccgatgagg ccgccgggg 
      acgtgccag tgccgggcc gaagagtgc aagtgataa 
      gataatcga tttggcgac gcgatacat tttcggtgc 
      actgataat tgagccgag gtgcgctgg gatcgcgcc 
      gtgtcatgt tctgagagg ttagggggc tcatggaga 
      agatggacg ttgatgaag ctgtcgtcg atctgagta 
      ttagttcaa accgatcag cttcaccag ccccacagc 
      agcagccgc agcaacagc agcaacaac aaccaatgg 
      gcattgtgt cgacaaata gcgcccagt cgatggtct 
      attcgagac gagtgcttc gcccccgca ctgaaccaa 
      gaagttacg tcgaaagcc ccgacgtgc tcattaatg 
      gcactctag ataaaccgc ggacgaacg gaaacgcgg 
      attatagca gcgattcga gcgaagggg aaatgcctc 
      cgttagcgc ccatcaagg agctcagcc cctcggagc 
      tgaagcaga gagacagag gcctgagac gactccgcg 
      aagatctcc gcgccgaag agatgaagc tggtgctgt 
      tgaaaaaat tgaaacaat cgcaacagc tgaaggaga 
      acgtggcgg tgttgcctc cgagcatac ctcagtcgg 
      cgttacctc cgaccggtc ttccaagcg gtctcagca 
      tcacaccga ccactgtca aagtaccat cgaacaaga 
      accaaccac cgcaatcgc ttcattccc gacatccga 
      attcgcaca agactggcc aaggaagtg ttccctcac 
      ttctgcgtg gagaccgat cgagggacg atggacaga 
      caccggcgc aaagacaag cggcggcga agttggccc 
      tacgaaaac aattggaaa agacgctac tacagatcc 
      ctccgccca agccgcctc cgcctgaaa tgcatttca 
      tccctaatc ctagcaaca ccgaattca tatatctgg 
      taggcttag aacacgtcg ttgatttcc tgacgaagg 
      agacgcgaa tgccgctgc cgccaatgc cgttttcgt 
      gtagtcaat gcaattctg attttaccc cggtttgga 
      agtgggaaa ctaaaggca aaaatagag ggattaaag 
      tcatttgtg agcaatgtg taaccaata acgtgaaga 
      aagcgctca aagccgaac atacgaacc ggctcaaga 
      cagcgtttg tgaaagctc ttcaacagg agcaggaaa 
      tcgagcaga ggcttgctc aaaatgggg ttttcactt 
      cgagcagtc cggcaccgt cgccagtaa ctccagaag 
      ccgttccta aatcggcga ccccgactc ctacgcctc 
      gacatgcgg ccactcccc cagcccctt ctgctcaac 
      aaacgcctc caccctcca ggagttcac actcgtcgt 
      cggataagt tctcgcaga atgcggcga tgcaagcgc 
      tgcaacaac agctgctca gaagcttga cggcgggag 
      ctccgccgg cgcacatgc tgccgttct cgccgctgt 
      tctaccctt accagatgg ccatagcgc aggcggccg 
      ccagcggca aagggggca caccgaaca tggctgagc 
      tgcagcggg ccgccgagc tgcaacgac agtacctcc 
      tcgacatga tccctccag gtccgggtc agcgtcaca 
      attggaaaa catgatgtg tcagtgata agatttaga 
      taatgacgt gctaaaaaa gtgatcgag actatgtat 
      aagaagaag ccatcgagg ctgtcatcc gcgaggatc 
      taaatgtcg atgtgccct tttgttcca gaagtaaaa 
      atcgctatt tattattta acagtttga gtggaaatt 
      tagtcaatt atcgcaaga ctgattcta gtacctatt 
      gtattaccg acactggag gttattgaa aaaatagaa 
      aaattaact cactaaacg aacggagat ctcaggatt 
      gacatactt acactaagt ttaaacgat ttatttcct 
      taacgttaa gttttcgga tctttttac ttctggaat 
      gtgttttat ttattacgt gatgttctc gagacagtg 
      caggacatg taaataagc tttggcgaa attttttag 
      atgtagaaa ttacggttg acaatgcta aagcagaga 
      cgtgtaagg ttgtttgta atcgtgtga ttgaattac 
      ttttcaacg gatttaagg cattcaacg tttacaatg 
      cacgattag ttgaatttt tttcgaggc atttatttt 
      taaagtgaa tgcttgaat gcggaatcc ttacattat 
      aacaagttt gtaaatagg tgtttttac aattgtata 
      atagttaat caataggaa tattgatgc atattatgc 
      tcattaatc ttgatgttg acatttatt atgaggtgt 
      tttctatta taacaaaaa cagtgtttt ctaagacaa 
      ctagctgaa aaaatgtac tttctgtaa aaaaagaaa 
      atatttatt tcagatgta atcataaaa gtggtattt 
      tcgaggcac cttaaatta ttcaatgca gctaaagga 
      atggaaact gaggggtga agagtagtt tttttttat 
      tggatttca ctgtactgt gttcatttt taatactgt 
      attttttcc attagaatc gtgtacatt ttagcgata 
      tgagggtgc taacagaaa tttcgattt ctgatgata 
      atctaaatg aatttttgt ttgtattaa tgggggagt 
      gtttggatt aacacaatt ttgtttttg cacattttt 
      cgtcacaaa actatgaag ctcaatgaa attattttc 
      taatctaaa aaatatcag taagactta tttagagca 
      caattatgc cagtaaaaa tatgtaaat gcatttcaa 
      atatctgca gtgtgaaaa atcgtctac ataataaaa 
      tcaacttct cagtcttac ttgtattac tagtgttat 
      tattgtagg tagtgcttg caatttttc catctaaac 
      tatagtcaa tctttgtcg gtaataaat cggctgtgg 
      aattatttt ttgattttc attttcttt ttcagtcat 
      acagtatca tacacaagc tgaacaggt ctttaggga 
      gctttataa actacagtt tgtaaataa cagtttcta 
      aaaactaaa tgcctcttc agtacttac gttaatatg 
      ttgcctcaa agttttcaa ctattctat tttgtaagt 
      gtataatta tttcaacat gtttttaaa taaattgac 
      tcataagtt
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