| General information | |||
| TC009782 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8087292..8107348 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009782 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04697 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature sometimes orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
female genital lobes shortened - Number of animals showing the phenotype: 8
abdomen gin-traps not present - Number of animals showing the phenotype: 8
- >TC009782 RA
MDVDEAVVD LSISSNRSA SPAPQQQPQ QQQQQQPMG IVSTNSAQS MVYSRRVLR PRTEPRSYV ESPDVLING TLDKPRTNG NADYSSDSS EGEMPPLAP IKELSPSEL KQRDRGLRR LREDLRAEE MKLVLLKKL KQSQQLKEN VAVLPPSIP QSALPPTGL PSGLSITPT TVKVPSNKN QPPQSLHSR HPNSHKTGQ GSVPSLLRG QPPPARSSI HAPPLSGSP LSSRGANLS VPQPPQRSS LGRPPSGFP PGVKDLSPS VTITPAPPQ PSKDRSRDD GQTPAQRQA AAKLALRKQ LEKTLLQIP PPKPPPPEM HFIPNPSNT EFIYLVGLE HVVDFLTKE TRMPLPPMP FSCSQCNSD FTPVWKWET KGKNRGIKV ICEQCVTNN VKKALKAEH TNRLKTAFV KALQQEQEI EQRLAQNGV FTSSSPAPS PVTPEAVPK SATPTPTPR HAATPPAPS AQQTPPPSR SSHSSSDKF SQNAAMQAL QQQLLRSLT AGAPPAHML PFSPLFYPY QMAIAQAAA SGKGGTPNM AELQRAAEL QRQYLLDMI PPGPGQRHN WKT
- >TC009782 RB
MDVDEAVVD LSISSNRSA SPAPQQQPQ QQQQQQPMG IVSTNSAQS MVYSRRVLR PRTEPRSYV ESPDVLING TLDKPRTNG NADYSSDSS EGEMPPLAP IKELSPSEL KQRDRGLRR LREDLRAEE MKLVLLKKL KQSQQLKEN VAVLPPSIP QSALPPTGL PSGLSITPT TVKVPSNKN QPPQSLHSR HPNSHKTGQ GSVPSLLRG DRSRDDGQT PAQRQAAAK LALRKQLEK TLLQIPPPK PPPPEMHFI PNPSNTEFI YLVGLEHVV DFLTKETRM PLPPMPFSC SQCNSDFTP VWKWETKGK NRGIKVICE QCVTNNVKK ALKAEHTNR LKTAFVKAL QQEQEIEQR LAQNGVFTS SSPAPSPVT PEAVPKSAT PTPTPRHAA TPPAPSAQQ TPPPSRSSH SSSDKFSQN AAMQALQQQ LLRSLTAGA PPAHMLPFS PLFYPYQMA IAQAAASGK GGTPNMAEL QRAAELQRQ YLLDMIPPG PGQRHNWKT
- >TC009782 RA
atggacgtt gatgaagct gtcgtcgat ctgagtatt agttcaaac cgatcagct tcaccagcc ccacagcag cagccgcag caacagcag caacaacaa ccaatgggc attgtgtcg acaaatagc gcccagtcg atggtctat tcgagacga gtgcttcgc ccccgcact gaaccaaga agttacgtc gaaagcccc gacgtgctc attaatggc actctagat aaaccgcgg acgaacgga aacgcggat tatagcagc gattcgagc gaaggggaa atgcctccg ttagcgccc atcaaggag ctcagcccc tcggagctg aagcagaga gacagaggc ctgagacga ctccgcgaa gatctccgc gccgaagag atgaagctg gtgctgttg aaaaaattg aaacaatcg caacagctg aaggagaac gtggcggtg ttgcctccg agcatacct cagtcggcg ttacctccg accggtctt ccaagcggt ctcagcatc acaccgacc actgtcaaa gtaccatcg aacaagaac caaccaccg caatcgctt cattcccga catccgaat tcgcacaag actggccaa ggaagtgtt ccctcactt ctgcgtgga caaccgccc ccagctcgt tcaagtatc cacgcgccg cctctgtcc ggctcgcct ctgagtagc cgaggcgcg aatctatcc gtacctcaa ccgccacaa aggagttct ttaggtcga cctccttca ggtttccct ccaggcgtt aaggatctc tcgccttcc gttacaatc acacccgca ccaccccaa ccgtctaag gaccgatcg agggacgat ggacagaca ccggcgcaa agacaagcg gcggcgaag ttggcccta cgaaaacaa ttggaaaag acgctacta cagatccct ccgcccaag ccgcctccg cctgaaatg catttcatc cctaatcct agcaacacc gaattcata tatctggta ggcttagaa cacgtcgtt gatttcctg acgaaggag acgcgaatg ccgctgccg ccaatgccg ttttcgtgt agtcaatgc aattctgat tttaccccg gtttggaag tgggaaact aaaggcaaa aatagaggg attaaagtc atttgtgag caatgtgta accaataac gtgaagaaa gcgctcaaa gccgaacat acgaaccgg ctcaagaca gcgtttgtg aaagctctt caacaggag caggaaatc gagcagagg cttgctcaa aatggggtt ttcacttcg agcagtccg gcaccgtcg ccagtaact ccagaagcc gttcctaaa tcggcgacc ccgactcct acgcctcga catgcggcc actccccca gccccttct gctcaacaa acgcctcca ccctccagg agttcacac tcgtcgtcg gataagttc tcgcagaat gcggcgatg caagcgctg caacaacag ctgctcaga agcttgacg gcgggagct ccgccggcg cacatgctg ccgttctcg ccgctgttc tacccttac cagatggcc atagcgcag gcggccgcc agcggcaaa gggggcaca ccgaacatg gctgagctg cagcgggcc gccgagctg caacgacag tacctcctc gacatgatc cctccaggt ccgggtcag cgtcacaat tggaaaaca tga
- >TC009782 RB
atggacgtt gatgaagct gtcgtcgat ctgagtatt agttcaaac cgatcagct tcaccagcc ccacagcag cagccgcag caacagcag caacaacaa ccaatgggc attgtgtcg acaaatagc gcccagtcg atggtctat tcgagacga gtgcttcgc ccccgcact gaaccaaga agttacgtc gaaagcccc gacgtgctc attaatggc actctagat aaaccgcgg acgaacgga aacgcggat tatagcagc gattcgagc gaaggggaa atgcctccg ttagcgccc atcaaggag ctcagcccc tcggagctg aagcagaga gacagaggc ctgagacga ctccgcgaa gatctccgc gccgaagag atgaagctg gtgctgttg aaaaaattg aaacaatcg caacagctg aaggagaac gtggcggtg ttgcctccg agcatacct cagtcggcg ttacctccg accggtctt ccaagcggt ctcagcatc acaccgacc actgtcaaa gtaccatcg aacaagaac caaccaccg caatcgctt cattcccga catccgaat tcgcacaag actggccaa ggaagtgtt ccctcactt ctgcgtgga gaccgatcg agggacgat ggacagaca ccggcgcaa agacaagcg gcggcgaag ttggcccta cgaaaacaa ttggaaaag acgctacta cagatccct ccgcccaag ccgcctccg cctgaaatg catttcatc cctaatcct agcaacacc gaattcata tatctggta ggcttagaa cacgtcgtt gatttcctg acgaaggag acgcgaatg ccgctgccg ccaatgccg ttttcgtgt agtcaatgc aattctgat tttaccccg gtttggaag tgggaaact aaaggcaaa aatagaggg attaaagtc atttgtgag caatgtgta accaataac gtgaagaaa gcgctcaaa gccgaacat acgaaccgg ctcaagaca gcgtttgtg aaagctctt caacaggag caggaaatc gagcagagg cttgctcaa aatggggtt ttcacttcg agcagtccg gcaccgtcg ccagtaact ccagaagcc gttcctaaa tcggcgacc ccgactcct acgcctcga catgcggcc actccccca gccccttct gctcaacaa acgcctcca ccctccagg agttcacac tcgtcgtcg gataagttc tcgcagaat gcggcgatg caagcgctg caacaacag ctgctcaga agcttgacg gcgggagct ccgccggcg cacatgctg ccgttctcg ccgctgttc tacccttac cagatggcc atagcgcag gcggccgcc agcggcaaa gggggcaca ccgaacatg gctgagctg cagcgggcc gccgagctg caacgacag tacctcctc gacatgatc cctccaggt ccgggtcag cgtcacaat tggaaaaca tga
- >TC009782 RA
gtgcgacaa agacggact cggtgaggt gcgtcgtgg cttgcgccg cgcgctgga tgaataaag gggcggccg gcgcgatct gcgacgtat gtcgtgaaa ggtaggagt gacgtagta aaagttcga ctgttcacg atacgtctg ttttatcaa cgcggtagt gtttgtaga cacaacaca aggccacgc catattgaa ttgaatcag tcgtctgtt aatttcgga gcggagtga agtgcggcc gattttcgt gttttgtgt gctgggggg ccgatgagg ccgccgggg acgtgccag tgccgggcc gaagagtgc aagtgataa gataatcga tttggcgac gcgatacat tttcggtgc actgataat tgagccgag gtgcgctgg gatcgcgcc gtgtcatgt tctgagagg ttagggggc tcatggaga agatggacg ttgatgaag ctgtcgtcg atctgagta ttagttcaa accgatcag cttcaccag ccccacagc agcagccgc agcaacagc agcaacaac aaccaatgg gcattgtgt cgacaaata gcgcccagt cgatggtct attcgagac gagtgcttc gcccccgca ctgaaccaa gaagttacg tcgaaagcc ccgacgtgc tcattaatg gcactctag ataaaccgc ggacgaacg gaaacgcgg attatagca gcgattcga gcgaagggg aaatgcctc cgttagcgc ccatcaagg agctcagcc cctcggagc tgaagcaga gagacagag gcctgagac gactccgcg aagatctcc gcgccgaag agatgaagc tggtgctgt tgaaaaaat tgaaacaat cgcaacagc tgaaggaga acgtggcgg tgttgcctc cgagcatac ctcagtcgg cgttacctc cgaccggtc ttccaagcg gtctcagca tcacaccga ccactgtca aagtaccat cgaacaaga accaaccac cgcaatcgc ttcattccc gacatccga attcgcaca agactggcc aaggaagtg ttccctcac ttctgcgtg gacaaccgc ccccagctc gttcaagta tccacgcgc cgcctctgt ccggctcgc ctctgagta gccgaggcg cgaatctat ccgtacctc aaccgccac aaaggagtt ctttaggtc gacctcctt caggtttcc ctccaggcg ttaaggatc tctcgcctt ccgttacaa tcacacccg caccacccc aaccgtcta aggaccgat cgagggacg atggacaga caccggcgc aaagacaag cggcggcga agttggccc tacgaaaac aattggaaa agacgctac tacagatcc ctccgccca agccgcctc cgcctgaaa tgcatttca tccctaatc ctagcaaca ccgaattca tatatctgg taggcttag aacacgtcg ttgatttcc tgacgaagg agacgcgaa tgccgctgc cgccaatgc cgttttcgt gtagtcaat gcaattctg attttaccc cggtttgga agtgggaaa ctaaaggca aaaatagag ggattaaag tcatttgtg agcaatgtg taaccaata acgtgaaga aagcgctca aagccgaac atacgaacc ggctcaaga cagcgtttg tgaaagctc ttcaacagg agcaggaaa tcgagcaga ggcttgctc aaaatgggg ttttcactt cgagcagtc cggcaccgt cgccagtaa ctccagaag ccgttccta aatcggcga ccccgactc ctacgcctc gacatgcgg ccactcccc cagcccctt ctgctcaac aaacgcctc caccctcca ggagttcac actcgtcgt cggataagt tctcgcaga atgcggcga tgcaagcgc tgcaacaac agctgctca gaagcttga cggcgggag ctccgccgg cgcacatgc tgccgttct cgccgctgt tctaccctt accagatgg ccatagcgc aggcggccg ccagcggca aagggggca caccgaaca tggctgagc tgcagcggg ccgccgagc tgcaacgac agtacctcc tcgacatga tccctccag gtccgggtc agcgtcaca attggaaaa catgatgtg tcagtgata agatttaga taatgacgt gctaaaaaa gtgatcgag actatgtat aagaagaag ccatcgagg ctgtcatcc gcgaggatc taaatgtcg atgtgccct tttgttcca gaagtaaaa atcgctatt tattattta acagtttga gtggaaatt tagtcaatt atcgcaaga ctgattcta gtacctatt gtattaccg acactggag gttattgaa aaaatagaa aaattaact cactaaacg aacggagat ctcaggatt gacatactt acactaagt ttaaacgat ttatttcct taacgttaa gttttcgga tctttttac ttctggaat gtgttttat ttattacgt gatgttctc gagacagtg caggacatg taaataagc tttggcgaa attttttag atgtagaaa ttacggttg acaatgcta aagcagaga cgtgtaagg ttgtttgta atcgtgtga ttgaattac ttttcaacg gatttaagg cattcaacg tttacaatg cacgattag ttgaatttt tttcgaggc atttatttt taaagtgaa tgcttgaat gcggaatcc ttacattat aacaagttt gtaaatagg tgtttttac aattgtata atagttaat caataggaa tattgatgc atattatgc tcattaatc ttgatgttg acatttatt atgaggtgt tttctatta taacaaaaa cagtgtttt ctaagacaa ctagctgaa aaaatgtac tttctgtaa aaaaagaaa atatttatt tcagatgta atcataaaa gtggtattt tcgaggcac cttaaatta ttcaatgca gctaaagga atggaaact gaggggtga agagtagtt tttttttat tggatttca ctgtactgt gttcatttt taatactgt attttttcc attagaatc gtgtacatt ttagcgata tgagggtgc taacagaaa tttcgattt ctgatgata atctaaatg aatttttgt ttgtattaa tgggggagt gtttggatt aacacaatt ttgtttttg cacattttt cgtcacaaa actatgaag ctcaatgaa attattttc taatctaaa aaatatcag taagactta tttagagca caattatgc cagtaaaaa tatgtaaat gcatttcaa atatctgca gtgtgaaaa atcgtctac ataataaaa tcaacttct cagtcttac ttgtattac tagtgttat tattgtagg tagtgcttg caatttttc catctaaac tatagtcaa tctttgtcg gtaataaat cggctgtgg aattatttt ttgattttc attttcttt ttcagtcat acagtatca tacacaagc tgaacaggt ctttaggga gctttataa actacagtt tgtaaataa cagtttcta aaaactaaa tgcctcttc agtacttac gttaatatg ttgcctcaa agttttcaa ctattctat tttgtaagt gtataatta tttcaacat gtttttaaa taaattgac tcataagtt
- >TC009782 RB
gtgcgacaa agacggact cggtgaggt gcgtcgtgg cttgcgccg cgcgctgga tgaataaag gggcggccg gcgcgatct gcgacgtat gtcgtgaaa ggtaggagt gacgtagta aaagttcga ctgttcacg atacgtctg ttttatcaa cgcggtagt gtttgtaga cacaacaca aggccacgc catattgaa ttgaatcag tcgtctgtt aatttcgga gcggagtga agtgcggcc gattttcgt gttttgtgt gctgggggg ccgatgagg ccgccgggg acgtgccag tgccgggcc gaagagtgc aagtgataa gataatcga tttggcgac gcgatacat tttcggtgc actgataat tgagccgag gtgcgctgg gatcgcgcc gtgtcatgt tctgagagg ttagggggc tcatggaga agatggacg ttgatgaag ctgtcgtcg atctgagta ttagttcaa accgatcag cttcaccag ccccacagc agcagccgc agcaacagc agcaacaac aaccaatgg gcattgtgt cgacaaata gcgcccagt cgatggtct attcgagac gagtgcttc gcccccgca ctgaaccaa gaagttacg tcgaaagcc ccgacgtgc tcattaatg gcactctag ataaaccgc ggacgaacg gaaacgcgg attatagca gcgattcga gcgaagggg aaatgcctc cgttagcgc ccatcaagg agctcagcc cctcggagc tgaagcaga gagacagag gcctgagac gactccgcg aagatctcc gcgccgaag agatgaagc tggtgctgt tgaaaaaat tgaaacaat cgcaacagc tgaaggaga acgtggcgg tgttgcctc cgagcatac ctcagtcgg cgttacctc cgaccggtc ttccaagcg gtctcagca tcacaccga ccactgtca aagtaccat cgaacaaga accaaccac cgcaatcgc ttcattccc gacatccga attcgcaca agactggcc aaggaagtg ttccctcac ttctgcgtg gagaccgat cgagggacg atggacaga caccggcgc aaagacaag cggcggcga agttggccc tacgaaaac aattggaaa agacgctac tacagatcc ctccgccca agccgcctc cgcctgaaa tgcatttca tccctaatc ctagcaaca ccgaattca tatatctgg taggcttag aacacgtcg ttgatttcc tgacgaagg agacgcgaa tgccgctgc cgccaatgc cgttttcgt gtagtcaat gcaattctg attttaccc cggtttgga agtgggaaa ctaaaggca aaaatagag ggattaaag tcatttgtg agcaatgtg taaccaata acgtgaaga aagcgctca aagccgaac atacgaacc ggctcaaga cagcgtttg tgaaagctc ttcaacagg agcaggaaa tcgagcaga ggcttgctc aaaatgggg ttttcactt cgagcagtc cggcaccgt cgccagtaa ctccagaag ccgttccta aatcggcga ccccgactc ctacgcctc gacatgcgg ccactcccc cagcccctt ctgctcaac aaacgcctc caccctcca ggagttcac actcgtcgt cggataagt tctcgcaga atgcggcga tgcaagcgc tgcaacaac agctgctca gaagcttga cggcgggag ctccgccgg cgcacatgc tgccgttct cgccgctgt tctaccctt accagatgg ccatagcgc aggcggccg ccagcggca aagggggca caccgaaca tggctgagc tgcagcggg ccgccgagc tgcaacgac agtacctcc tcgacatga tccctccag gtccgggtc agcgtcaca attggaaaa catgatgtg tcagtgata agatttaga taatgacgt gctaaaaaa gtgatcgag actatgtat aagaagaag ccatcgagg ctgtcatcc gcgaggatc taaatgtcg atgtgccct tttgttcca gaagtaaaa atcgctatt tattattta acagtttga gtggaaatt tagtcaatt atcgcaaga ctgattcta gtacctatt gtattaccg acactggag gttattgaa aaaatagaa aaattaact cactaaacg aacggagat ctcaggatt gacatactt acactaagt ttaaacgat ttatttcct taacgttaa gttttcgga tctttttac ttctggaat gtgttttat ttattacgt gatgttctc gagacagtg caggacatg taaataagc tttggcgaa attttttag atgtagaaa ttacggttg acaatgcta aagcagaga cgtgtaagg ttgtttgta atcgtgtga ttgaattac ttttcaacg gatttaagg cattcaacg tttacaatg cacgattag ttgaatttt tttcgaggc atttatttt taaagtgaa tgcttgaat gcggaatcc ttacattat aacaagttt gtaaatagg tgtttttac aattgtata atagttaat caataggaa tattgatgc atattatgc tcattaatc ttgatgttg acatttatt atgaggtgt tttctatta taacaaaaa cagtgtttt ctaagacaa ctagctgaa aaaatgtac tttctgtaa aaaaagaaa atatttatt tcagatgta atcataaaa gtggtattt tcgaggcac cttaaatta ttcaatgca gctaaagga atggaaact gaggggtga agagtagtt tttttttat tggatttca ctgtactgt gttcatttt taatactgt attttttcc attagaatc gtgtacatt ttagcgata tgagggtgc taacagaaa tttcgattt ctgatgata atctaaatg aatttttgt ttgtattaa tgggggagt gtttggatt aacacaatt ttgtttttg cacattttt cgtcacaaa actatgaag ctcaatgaa attattttc taatctaaa aaatatcag taagactta tttagagca caattatgc cagtaaaaa tatgtaaat gcatttcaa atatctgca gtgtgaaaa atcgtctac ataataaaa tcaacttct cagtcttac ttgtattac tagtgttat tattgtagg tagtgcttg caatttttc catctaaac tatagtcaa tctttgtcg gtaataaat cggctgtgg aattatttt ttgattttc attttcttt ttcagtcat acagtatca tacacaagc tgaacaggt ctttaggga gctttataa actacagtt tgtaaataa cagtttcta aaaactaaa tgcctcttc agtacttac gttaatatg ttgcctcaa agttttcaa ctattctat tttgtaagt gtataatta tttcaacat gtttttaaa taaattgac tcataagtt
