Status
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Gene information
General information
TC009779 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8046992..8066551
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009779
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva appendages irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs sometimes shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009779 RA
      MKPGELKRV LFFFSFCCI LFFIYLYWQ QTSNLTTSK 
      LLYTYRQPY PRQKKSHPP QWTWQCINQ RCERRHIKG 
      AIPVVSLST CSMLCGSTQ LWPQPTGPV TLASRAVTF 
      NHQQLELET DTPEPARTL LEHSFVAFN TNIISLVQN 
      KDYVERDTD IRRFLIKVT ILHPNIVKL KLDTSEGYT 
      LSVKPRDGE IVANITAKT FFGARHGLE TLSQLIWWD 
      DYETKGALK VLKGATVQD NPIFPYRGI MLDTARNYM 
      SVESIRRVL DGMAANKLN VFHWHLTDS QSFPLVSQR 
      VPQLAKNGA YGPDMIYTP EDVKALVEY ARIRGIRVV 
      LEVDTPAHA GNGWNWGPQ EGLGELAVC VNERPWSLY 
      CGEPPCGQL NPDNPNVYE ILEKLYKDL LELSDETEL 
      FHLGGDEVN LECWAQHLQ KTTTFMNYT DLHDLWGEF 
      TLKALKRLE RANNGVKIP LVIIWSSNL SKRPYIYKY 
      LDKKNVVVQ SWGASQWPD TPDLISDGY RVIISHVDA 
      WYLDCGFGR WRETGEAAC DPYRPWQTV YNHRPWQQL 
      HLNKKQILG GEACLWSEQ FDETSLDTR LWPRAAAFA 
      ERVWSDPQL DVTSFTIQE DVYTRLNTH RDRLVARGL 
      GAEAMWPVW CAQNPGMCL
    CDS sequence
    • >TC009779 RA
      atgaagcct ggcgagctt aagcgtgtc ctcttcttt 
      ttttccttc tgctgtatt ttattcttc atttatctg 
      tactggcaa caaaccagc aatcttacc acgtcgaag 
      cttttgtac acttataga caaccctac ccccggcag 
      aagaagtcg cacccgccc cagtggact tggcaatgt 
      ataaatcag cgatgcgag aggcggcat ataaaaggg 
      gccatccct gtagtgtca ttatcgaca tgcagcatg 
      ttgtgcggc tcgacgcag ctctggccc caacccacc 
      ggacctgta actctcgcc agcagggct gtcacattc 
      aaccatcaa caattagag ctcgaaacg gacactccc 
      gaacccgct agaacactt ttagagcat tctttcgtg 
      gccttcaat actaacatt atcagtcta gtccaaaac 
      aaagattac gtagaacga gacaccgac attagaaga 
      tttctaatc aaagtcaca attttgcac ccgaatatc 
      gtaaaacta aagctggat acgagcgaa ggctatacg 
      ttgagtgtg aagccacgg gacggcgaa attgtggcc 
      aatatcacg gcgaaaact tttttcggg gccaggcac 
      gggctcgag actttgtcc cagttgatt tggtgggac 
      gattatgaa accaagggg gcgctcaag gtacttaaa 
      ggggccacg gtccaggat aatcccatt tttccctac 
      cgtgggatt atgctggat acggcgagg aattacatg 
      agtgttgag agtattcga cgagtgttg gacggaatg 
      gccgcgaat aaactcaat gtcttccac tggcacctc 
      accgactcc cagagtttt cctttggtg tcacaaaga 
      gtgcctcag ttggccaaa aacggtgcc tacggaccc 
      gacatgatc tacacgccg gaagacgtc aaggctttg 
      gtcgaatat gcccgaatc agaggcatc cgagtggtg 
      cttgaagtg gatacaccg gcccacgcc ggcaatggt 
      tggaattgg ggcccccag gaagggcta ggcgaacta 
      gcagtgtgt gtcaacgag cgcccctgg agcctgtac 
      tgcggggag cccccatgt ggccaactc aaccccgac 
      aaccccaac gtttacgaa atcctcgag aaactgtac 
      aaagacttg ttagaatta agtgacgaa acggagctc 
      ttccatttg ggaggagac gaggtgaat ttggagtgt 
      tgggcccaa cacttgcaa aaaaccacg actttcatg 
      aactacacc gacttgcac gacctttgg ggcgagttc 
      accctcaag gcgttaaaa cgattggaa cgggccaat 
      aacggggta aagatcccg cttgtgata atctggtcc 
      agcaatctc agcaagaga ccctacatt tacaaatat 
      ttagataag aaaaacgtc gtggtgcag agttggggg 
      gcgagccag tggccggac acccccgac ctcatcagc 
      gacggctac cgggtgatc atctcgcac gtggacgcg 
      tggtacctc gactgcggt ttcgggcgg tggcgtgag 
      acgggggag gcggcgtgt gacccatac cggccgtgg 
      cagaccgtc tacaaccac cggccgtgg cagcaactg 
      cacctcaac aagaagcag atcctgggg ggcgaggcc 
      tgtctctgg agcgagcag ttcgacgag acgtcgctc 
      gatacgagg ctgtggccg agggcggcc gccttcgcc 
      gagagggtg tggagcgac ccgcagctg gatgtaacc 
      tcgtttacc atccaggag gacgtctac acgaggttg 
      aacacgcat agggaccgg ttggtggcc cgggggctg 
      ggggcggag gccatgtgg ccggtgtgg tgtgcgcaa 
      aatcccggt atgtgcctt tga
    mRNA sequence
    • >TC009779 RA
      taacaataa tccaggtaa atacaatgc agctgacaa 
      agtagtgtg gttgagccg ttgagcctc ttgaattta 
      aaacatggt tacatttgt tgtggaaag gtttattta 
      tttagtagg taaactttg taactttag cttgacaaa 
      cctgtaaat attttaaga ggtaataac gtgtgttaa 
      agtcattgc ttcaattgt ttggacttg agcaaataa 
      aaaaattaa atattcagg taaacaata cagtcgcat 
      tctgacacg ggtttgtgt agcgtggaa aagtgttcg 
      gaagtactc tgatgcagt gatgaaatg tatctattg 
      tttccatgt ttgccggca tgtgattgt aaaatattt 
      attttcgct atttacata aagtcagtg agcctttca 
      tatctaaac atgtataat tcaaactcg agttcaata 
      caatcctct ttgaactac gaagtggta aaaatgcga 
      aggtgaact aagcaccag ccggggtca tgatttagt 
      gtggacaaa aaatgataa cgctgaggc tccagacaa 
      tttgaattt caaattccc cttttttcg ttgtctcct 
      tccaagcga actcaacag gccccagct ccgccaaat 
      tcgcgtcga cctagttcg gtcacttcc actctccgc 
      caagcgctc gagtgtatg accagccgt acaatgacg 
      tcttctgcg aagcggcca ccgcgtttc accgagatg 
      gttcgccgt gaccatgga ggatgttaa cgacgcgaa 
      ccagttcga atccgtgca agtttcgcg ccgcacgtg 
      tatggaaaa acgcgactt ttcaattag atccccgaa 
      ccccaccca aggaaaccg tacgagttc cgtaaatag 
      agtgtagtg acttcagca ttgcgtaaa tgaagacaa 
      ccacgcgaa aaataaatc ggctcggtt gggtgcctc 
      ttgcagtca gcgtctgcg gtccaaggc cggtgccag 
      agaaactgc ccagtgccc atcttggta tctacctgt 
      gctcaggtg caagcaaca tgatacaga ctctcactc 
      cggctacca acgactctc tcttcaact ccgaactgt 
      gatagtgtg tgtgcaaat gctgataaa acgataaca 
      caggctcga gaggatgaa gcctggcga gcttaagcg 
      tgtcctctt ctttttttc cttctgctg tattttatt 
      cttcattta tctgtactg gcaacaaac cagcaatct 
      taccacgtc gaagctttt gtacactta tagacaacc 
      ctacccccg gcagaagaa gtcgcaccc gccccagtg 
      gacttggca atgtataaa tcagcgatg cgagaggcg 
      gcatataaa aggggccat ccctgtagt gtcattatc 
      gacatgcag catgttgtg cggctcgac gcagctctg 
      gccccaacc caccggacc tgtaactct cgccagcag 
      ggctgtcac attcaacca tcaacaatt agagctcga 
      aacggacac tcccgaacc cgctagaac acttttaga 
      gcattcttt cgtggcctt caatactaa cattatcag 
      tctagtcca aaacaaaga ttacgtaga acgagacac 
      cgacattag aagatttct aatcaaagt cacaatttt 
      gcacccgaa tatcgtaaa actaaagct ggatacgag 
      cgaaggcta tacgttgag tgtgaagcc acgggacgg 
      cgaaattgt ggccaatat cacggcgaa aactttttt 
      cggggccag gcacgggct cgagacttt gtcccagtt 
      gatttggtg ggacgatta tgaaaccaa gggggcgct 
      caaggtact taaaggggc cacggtcca ggataatcc 
      catttttcc ctaccgtgg gattatgct ggatacggc 
      gaggaatta catgagtgt tgagagtat tcgacgagt 
      gttggacgg aatggccgc gaataaact caatgtctt 
      ccactggca cctcaccga ctcccagag ttttccttt 
      ggtgtcaca aagagtgcc tcagttggc caaaaacgg 
      tgcctacgg acccgacat gatctacac gccggaaga 
      cgtcaaggc tttggtcga atatgcccg aatcagagg 
      catccgagt ggtgcttga agtggatac accggccca 
      cgccggcaa tggttggaa ttggggccc ccaggaagg 
      gctaggcga actagcagt gtgtgtcaa cgagcgccc 
      ctggagcct gtactgcgg ggagccccc atgtggcca 
      actcaaccc cgacaaccc caacgttta cgaaatcct 
      cgagaaact gtacaaaga cttgttaga attaagtga 
      cgaaacgga gctcttcca tttgggagg agacgaggt 
      gaatttgga gtgttgggc ccaacactt gcaaaaaac 
      cacgacttt catgaacta caccgactt gcacgacct 
      ttggggcga gttcaccct caaggcgtt aaaacgatt 
      ggaacgggc caataacgg ggtaaagat cccgcttgt 
      gataatctg gtccagcaa tctcagcaa gagacccta 
      catttacaa atatttaga taagaaaaa cgtcgtggt 
      gcagagttg gggggcgag ccagtggcc ggacacccc 
      cgacctcat cagcgacgg ctaccgggt gatcatctc 
      gcacgtgga cgcgtggta cctcgactg cggtttcgg 
      gcggtggcg tgagacggg ggaggcggc gtgtgaccc 
      ataccggcc gtggcagac cgtctacaa ccaccggcc 
      gtggcagca actgcacct caacaagaa gcagatcct 
      ggggggcga ggcctgtct ctggagcga gcagttcga 
      cgagacgtc gctcgatac gaggctgtg gccgagggc 
      ggccgcctt cgccgagag ggtgtggag cgacccgca 
      gctggatgt aacctcgtt taccatcca ggaggacgt 
      ctacacgag gttgaacac gcataggga ccggttggt 
      ggcccgggg gctgggggc ggaggccat gtggccggt 
      gtggtgtgc gcaaaatcc cggtatgtg cctttgacg 
      ggggaccaa aggactgtt tatgtgcca atgtgtgac 
      tgaaggacg ctcttgccg agtacttac agccaaagg 
      ttcgattcg tcagtacaa ttagggtta agtggatca 
      gcaagtgct tatttattg aataaaaat tttaagtaa 
      at
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