Status
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Gene information
General information
TC009768 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7839513..7932500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009768
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04694

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009768 RA
      MIVFMGLIW RILAIYLVT APQVSPIFN GLEDTSTPN 
      DLDKPVPMV EVQGVLNKK KGLPCDITP KERDDAVAM 
      VLWFKETIG EPLYSFDVR ARQFSQAKY WSSPHVFGD 
      RAYFRHTTS PATLEIASI QLSDEGIYR CRVDFKNSP 
      TRNSKVNFT VIVPPEKIH IYDDKRVDK TILLGPYNE 
      GSDVNLLCE VKGGRPRPK VTWFLENTV IDDSYEIRS 
      DGIAVNHLT FPNVGRQHL HARLICQAS NNNLVPPET 
      RVAVLDINL KPQTVNILT KEKQVSAGK RYEVECRTS 
      GSRPDAVIT WWKGSRPVR KLAKNFSEQ NNQSLSILT 
      FVPVIDDDG KYLTCRAEN PSIPESALE DKWRLNVHY 
      TPVVTLKMG STLNPDDIK EGDDVYFEC NIRANPKAY 
      KLSWFHDGV EIFQNVTAG VIMSDQSLV LQSVTRATG 
      GAYTCMATN VEGKSTSNA VQLVVRYAP VCKQDREEL 
      YGALKQETV TLRCQVDAN PAIVTFHWT FNNSGDLTE 
      VPANRFTNE LSSSRLNYT PVSDLDYGT LLCWGDNDV 
      GHQKVPCVF QVVIAGRPS QLQNCTVSN QSSDSLQVD 
      CTEGFDGGL PQNFFMEIL ELPALRSRL NVTSYRTPP 
      TFFADGLDP GVSYRIMLY AVNAKGRSE ATIIDPVTF 
      KGVAKLQGS TASMPVSPL IMGLLGTAG VLAAGVCLV 
      LAALCRRHY SRPCSRPEG GTKHVPMEA VIAADDLIV 
      DGSVTGVRT PATPDAAIT AEAVRNGTS IEATDPDII 
      RNQYERRPT LGFMKVYEP PGSREEEDV EDEGEEYDF 
      RHVAKETHI PNQTVYRSL QRPGRNPAN PIPGTQTLT 
      HKYRGPEVI TTSNRIQES CI
    CDS sequence
    • >TC009768 RA
      atgatagtg ttcatgggg ttgatttgg agaatcctt 
      gccatctat ttggtgacc gcgcctcag gtgtctccc 
      atcttcaac gggctagag gatacttcc acccccaac 
      gacctcgat aagcccgtt ccaatggtg gaagttcaa 
      ggagtgttg aataaaaag aagggtctt ccctgtgat 
      ataaccccg aaggagcgt gatgatgcc gtcgccatg 
      gtgctatgg ttcaaggag accatcggc gagcctctt 
      tacagtttc gacgtaagg gctcgacag ttcagtcag 
      gcaaaatat tggtcgtca ccacacgtg ttcggagac 
      agggcgtat tttcggcac accacgtca cctgcaaca 
      ttagaaatt gcatcaatt caactctca gatgaagga 
      atctaccga tgccgagtc gattttaaa aactcaccc 
      accagaaat agtaaagtc aacttcact gttatcgtg 
      cctcccgaa aagatccac atttacgat gacaagcga 
      gtcgacaag acgattcta ttaggacca tataatgag 
      ggctcggac gttaattta ttgtgcgaa gttaaggga 
      ggccgacct cgaccgaag gtgacgtgg tttctcgaa 
      aacacagtg atcgatgat tcgtacgag ataagatcg 
      gatggcata gccgtcaat catctcaca tttccaaat 
      gttggtcgc caacattta catgcaaga ctgatttgc 
      caggccagt aataacaat ctggtccca cctgagaca 
      agagtagct gtgttagac attaattta aaaccccaa 
      acggtaaat atcctaaca aaagaaaaa caagtgtca 
      gcaggcaaa cgctacgaa gttgagtgt aggacgtct 
      ggatcgagg ccagacgca gttataaca tggtggaaa 
      ggaagcaga cctgtcaga aaactagca aaaaacttt 
      tcggaacag aacaaccaa agcttaagc attttgacg 
      tttgtccct gtgatcgat gacgacggc aaatacttg 
      acctgtcga gctgaaaac ccgtccatc cctgaaagc 
      gcgctcgag gacaagtgg agattaaac gtccattat 
      actcctgtt gtaacgtta aaaatgggc tcgacatta 
      aacccggac gatatcaaa gaaggtgac gacgtttat 
      ttcgaatgc aatatccgg gccaacccc aaagcttac 
      aaactttcc tggttccac gacggagtg gagattttc 
      cagaacgtc accgccgga gtgatcatg agcgatcaa 
      agtttggtg ttgcagagc gtaacgaga gccacggga 
      ggtgcttac acgtgtatg gccaccaac gtggaaggc 
      aaaagcacc agtaatgcc gtccaactt gtcgtgaga 
      tacgccccc gtgtgcaaa caggaccga gaggagtta 
      tatggcgcg ttgaagcaa gagaccgtg actttgcgc 
      tgtcaggtc gacgccaat cctgccata gtgaccttc 
      cactggacg ttcaacaac tccggggac ctgaccgaa 
      gttccggcc aacaggttc acaaacgag ctctcttcc 
      tcccgactc aactacaca cccgtctcg gacctcgac 
      tacggcaca ctcctctgt tggggtgac aacgatgtg 
      ggtcaccag aaggtcccc tgcgtcttc caagtagtc 
      atagcaggc cggccgtcg cagctgcaa aattgcacc 
      gtatcgaac cagagctcg gattcgctc caagtggac 
      tgcacagag ggcttcgat ggtgggctc cctcagaac 
      ttcttcatg gagatcctg gaactgcca gcgcttcgg 
      tcccgactg aacgtcacc agctaccgg actcctccg 
      acgtttttc gccgacggc ctggatccg ggagtgagc 
      taccggatc atgctttac gccgtgaat gccaaggga 
      aggagcgaa gccaccatt attgacccc gtcacgttc 
      aaaggtgtc gccaaatta cagggttca acggcaagt 
      atgccggtt agcccccta attatgggt cttctgggc 
      actgccggc gtgctggcg gcgggggtc tgcctcgtg 
      ctggctgct ctgtgccgg agacactac tccaggccg 
      tgctcgagg cccgaaggg ggcaccaag cacgtacct 
      atggaagcg gttattgcc gctgacgat ctcatcgtt 
      gacggctcc gtcacgggc gtacgcaca ccagcgact 
      cccgacgcg gccattacc gccgaagcc gtgagaaac 
      ggcacttcc atagaggcc acagatcca gacattata 
      cgaaatcaa tatgagcgt cgaccgacc ctcggattt 
      atgaaagtg tacgagccg cccgggtcc agagaggaa 
      gaggacgtc gaagacgag ggcgaggag tacgatttc 
      agacacgtt gccaaagaa actcacatc cccaatcag 
      accgtgtac cggagtctg cagaggccc ggacgaaac 
      cccgcaaac cccattccg ggcactcag accttgacg 
      cacaagtat cgagggccc gaagtcatt acgacgtcg 
      aaccgaata caggagagc tgcatataa
    mRNA sequence
    • >TC009768 RA
      gatgaccgc gaaagagat cgtttcgaa cggcgtcga 
      aggcccgga aagccgcaa ggataatat gccatgccc 
      gcaaacata acccctcaa aggacgaag ctttcaaac 
      tttgcaact cgtgctgat tctttacga tgaattgcg 
      agattttgt ggccaaacg gtgcaggat tataaagcg 
      ttgtgccgg gtcctgtgt ggtgttgtg agatatttc 
      agtgttgag atgtaagga aatgatagt gttcatggg 
      gttgatttg gagaatcct tgccatcta tttggtgac 
      cgcgcctca ggtgtctcc catcttcaa cgggctaga 
      ggatacttc cacccccaa cgacctcga taagcccgt 
      tccaatggt ggaagttca aggagtgtt gaataaaaa 
      gaagggtct tccctgtga tataacccc gaaggagcg 
      tgatgatgc cgtcgccat ggtgctatg gttcaagga 
      gaccatcgg cgagcctct ttacagttt cgacgtaag 
      ggctcgaca gttcagtca ggcaaaata ttggtcgtc 
      accacacgt gttcggaga cagggcgta ttttcggca 
      caccacgtc acctgcaac attagaaat tgcatcaat 
      tcaactctc agatgaagg aatctaccg atgccgagt 
      cgattttaa aaactcacc caccagaaa tagtaaagt 
      caacttcac tgttatcgt gcctcccga aaagatcca 
      catttacga tgacaagcg agtcgacaa gacgattct 
      attaggacc atataatga gggctcgga cgttaattt 
      attgtgcga agttaaggg aggccgacc tcgaccgaa 
      ggtgacgtg gtttctcga aaacacagt gatcgatga 
      ttcgtacga gataagatc ggatggcat agccgtcaa 
      tcatctcac atttccaaa tgttggtcg ccaacattt 
      acatgcaag actgatttg ccaggccag taataacaa 
      tctggtccc acctgagac aagagtagc tgtgttaga 
      cattaattt aaaacccca aacggtaaa tatcctaac 
      aaaagaaaa acaagtgtc agcaggcaa acgctacga 
      agttgagtg taggacgtc tggatcgag gccagacgc 
      agttataac atggtggaa aggaagcag acctgtcag 
      aaaactagc aaaaaactt ttcggaaca gaacaacca 
      aagcttaag cattttgac gtttgtccc tgtgatcga 
      tgacgacgg caaatactt gacctgtcg agctgaaaa 
      cccgtccat ccctgaaag cgcgctcga ggacaagtg 
      gagattaaa cgtccatta tactcctgt tgtaacgtt 
      aaaaatggg ctcgacatt aaacccgga cgatatcaa 
      agaaggtga cgacgttta tttcgaatg caatatccg 
      ggccaaccc caaagctta caaactttc ctggttcca 
      cgacggagt ggagatttt ccagaacgt caccgccgg 
      agtgatcat gagcgatca aagtttggt gttgcagag 
      cgtaacgag agccacggg aggtgctta cacgtgtat 
      ggccaccaa cgtggaagg caaaagcac cagtaatgc 
      cgtccaact tgtcgtgag atacgcccc cgtgtgcaa 
      acaggaccg agaggagtt atatggcgc gttgaagca 
      agagaccgt gactttgcg ctgtcaggt cgacgccaa 
      tcctgccat agtgacctt ccactggac gttcaacaa 
      ctccgggga cctgaccga agttccggc caacaggtt 
      cacaaacga gctctcttc ctcccgact caactacac 
      acccgtctc ggacctcga ctacggcac actcctctg 
      ttggggtga caacgatgt gggtcacca gaaggtccc 
      ctgcgtctt ccaagtagt catagcagg ccggccgtc 
      gcagctgca aaattgcac cgtatcgaa ccagagctc 
      ggattcgct ccaagtgga ctgcacaga gggcttcga 
      tggtgggct ccctcagaa cttcttcat ggagatcct 
      ggaactgcc agcgcttcg gtcccgact gaacgtcac 
      cagctaccg gactcctcc gacgttttt cgccgacgg 
      cctggatcc gggagtgag ctaccggat catgcttta 
      cgccgtgaa tgccaaggg aaggagcga agccaccat 
      tattgaccc cgtcacgtt caaaggtgt cgccaaatt 
      acagggttc aacggcaag tatgccggt tagccccct 
      aattatggg tcttctggg cactgccgg cgtgctggc 
      ggcgggggt ctgcctcgt gctggctgc tctgtgccg 
      gagacacta ctccaggcc gtgctcgag gcccgaagg 
      gggcaccaa gcacgtacc tatggaagc ggttattgc 
      cgctgacga tctcatcgt tgacggctc cgtcacggg 
      cgtacgcac accagcgac tcccgacgc ggccattac 
      cgccgaagc cgtgagaaa cggcacttc catagaggc 
      cacagatcc agacattat acgaaatca atatgagcg 
      tcgaccgac cctcggatt tatgaaagt gtacgagcc 
      gcccgggtc cagagagga agaggacgt cgaagacga 
      gggcgagga gtacgattt cagacacgt tgccaaaga 
      aactcacat ccccaatca gaccgtgta ccggagtct 
      gcagaggcc cggacgaaa ccccgcaaa ccccattcc 
      gggcactca gaccttgac gcacaagta tcgagggcc 
      cgaagtcat tacgacgtc gaaccgaat acaggagag 
      ctgcatata aatgtaatt gtttttatc atgtattat 
      attaatgta tttaaaata aataaaagc ggactttca 
      tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY