| General information | |||
| TC009768 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 7839513..7932500 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009768 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04694 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC009768 RA
MIVFMGLIW RILAIYLVT APQVSPIFN GLEDTSTPN DLDKPVPMV EVQGVLNKK KGLPCDITP KERDDAVAM VLWFKETIG EPLYSFDVR ARQFSQAKY WSSPHVFGD RAYFRHTTS PATLEIASI QLSDEGIYR CRVDFKNSP TRNSKVNFT VIVPPEKIH IYDDKRVDK TILLGPYNE GSDVNLLCE VKGGRPRPK VTWFLENTV IDDSYEIRS DGIAVNHLT FPNVGRQHL HARLICQAS NNNLVPPET RVAVLDINL KPQTVNILT KEKQVSAGK RYEVECRTS GSRPDAVIT WWKGSRPVR KLAKNFSEQ NNQSLSILT FVPVIDDDG KYLTCRAEN PSIPESALE DKWRLNVHY TPVVTLKMG STLNPDDIK EGDDVYFEC NIRANPKAY KLSWFHDGV EIFQNVTAG VIMSDQSLV LQSVTRATG GAYTCMATN VEGKSTSNA VQLVVRYAP VCKQDREEL YGALKQETV TLRCQVDAN PAIVTFHWT FNNSGDLTE VPANRFTNE LSSSRLNYT PVSDLDYGT LLCWGDNDV GHQKVPCVF QVVIAGRPS QLQNCTVSN QSSDSLQVD CTEGFDGGL PQNFFMEIL ELPALRSRL NVTSYRTPP TFFADGLDP GVSYRIMLY AVNAKGRSE ATIIDPVTF KGVAKLQGS TASMPVSPL IMGLLGTAG VLAAGVCLV LAALCRRHY SRPCSRPEG GTKHVPMEA VIAADDLIV DGSVTGVRT PATPDAAIT AEAVRNGTS IEATDPDII RNQYERRPT LGFMKVYEP PGSREEEDV EDEGEEYDF RHVAKETHI PNQTVYRSL QRPGRNPAN PIPGTQTLT HKYRGPEVI TTSNRIQES CI
- >TC009768 RA
atgatagtg ttcatgggg ttgatttgg agaatcctt gccatctat ttggtgacc gcgcctcag gtgtctccc atcttcaac gggctagag gatacttcc acccccaac gacctcgat aagcccgtt ccaatggtg gaagttcaa ggagtgttg aataaaaag aagggtctt ccctgtgat ataaccccg aaggagcgt gatgatgcc gtcgccatg gtgctatgg ttcaaggag accatcggc gagcctctt tacagtttc gacgtaagg gctcgacag ttcagtcag gcaaaatat tggtcgtca ccacacgtg ttcggagac agggcgtat tttcggcac accacgtca cctgcaaca ttagaaatt gcatcaatt caactctca gatgaagga atctaccga tgccgagtc gattttaaa aactcaccc accagaaat agtaaagtc aacttcact gttatcgtg cctcccgaa aagatccac atttacgat gacaagcga gtcgacaag acgattcta ttaggacca tataatgag ggctcggac gttaattta ttgtgcgaa gttaaggga ggccgacct cgaccgaag gtgacgtgg tttctcgaa aacacagtg atcgatgat tcgtacgag ataagatcg gatggcata gccgtcaat catctcaca tttccaaat gttggtcgc caacattta catgcaaga ctgatttgc caggccagt aataacaat ctggtccca cctgagaca agagtagct gtgttagac attaattta aaaccccaa acggtaaat atcctaaca aaagaaaaa caagtgtca gcaggcaaa cgctacgaa gttgagtgt aggacgtct ggatcgagg ccagacgca gttataaca tggtggaaa ggaagcaga cctgtcaga aaactagca aaaaacttt tcggaacag aacaaccaa agcttaagc attttgacg tttgtccct gtgatcgat gacgacggc aaatacttg acctgtcga gctgaaaac ccgtccatc cctgaaagc gcgctcgag gacaagtgg agattaaac gtccattat actcctgtt gtaacgtta aaaatgggc tcgacatta aacccggac gatatcaaa gaaggtgac gacgtttat ttcgaatgc aatatccgg gccaacccc aaagcttac aaactttcc tggttccac gacggagtg gagattttc cagaacgtc accgccgga gtgatcatg agcgatcaa agtttggtg ttgcagagc gtaacgaga gccacggga ggtgcttac acgtgtatg gccaccaac gtggaaggc aaaagcacc agtaatgcc gtccaactt gtcgtgaga tacgccccc gtgtgcaaa caggaccga gaggagtta tatggcgcg ttgaagcaa gagaccgtg actttgcgc tgtcaggtc gacgccaat cctgccata gtgaccttc cactggacg ttcaacaac tccggggac ctgaccgaa gttccggcc aacaggttc acaaacgag ctctcttcc tcccgactc aactacaca cccgtctcg gacctcgac tacggcaca ctcctctgt tggggtgac aacgatgtg ggtcaccag aaggtcccc tgcgtcttc caagtagtc atagcaggc cggccgtcg cagctgcaa aattgcacc gtatcgaac cagagctcg gattcgctc caagtggac tgcacagag ggcttcgat ggtgggctc cctcagaac ttcttcatg gagatcctg gaactgcca gcgcttcgg tcccgactg aacgtcacc agctaccgg actcctccg acgtttttc gccgacggc ctggatccg ggagtgagc taccggatc atgctttac gccgtgaat gccaaggga aggagcgaa gccaccatt attgacccc gtcacgttc aaaggtgtc gccaaatta cagggttca acggcaagt atgccggtt agcccccta attatgggt cttctgggc actgccggc gtgctggcg gcgggggtc tgcctcgtg ctggctgct ctgtgccgg agacactac tccaggccg tgctcgagg cccgaaggg ggcaccaag cacgtacct atggaagcg gttattgcc gctgacgat ctcatcgtt gacggctcc gtcacgggc gtacgcaca ccagcgact cccgacgcg gccattacc gccgaagcc gtgagaaac ggcacttcc atagaggcc acagatcca gacattata cgaaatcaa tatgagcgt cgaccgacc ctcggattt atgaaagtg tacgagccg cccgggtcc agagaggaa gaggacgtc gaagacgag ggcgaggag tacgatttc agacacgtt gccaaagaa actcacatc cccaatcag accgtgtac cggagtctg cagaggccc ggacgaaac cccgcaaac cccattccg ggcactcag accttgacg cacaagtat cgagggccc gaagtcatt acgacgtcg aaccgaata caggagagc tgcatataa
- >TC009768 RA
gatgaccgc gaaagagat cgtttcgaa cggcgtcga aggcccgga aagccgcaa ggataatat gccatgccc gcaaacata acccctcaa aggacgaag ctttcaaac tttgcaact cgtgctgat tctttacga tgaattgcg agattttgt ggccaaacg gtgcaggat tataaagcg ttgtgccgg gtcctgtgt ggtgttgtg agatatttc agtgttgag atgtaagga aatgatagt gttcatggg gttgatttg gagaatcct tgccatcta tttggtgac cgcgcctca ggtgtctcc catcttcaa cgggctaga ggatacttc cacccccaa cgacctcga taagcccgt tccaatggt ggaagttca aggagtgtt gaataaaaa gaagggtct tccctgtga tataacccc gaaggagcg tgatgatgc cgtcgccat ggtgctatg gttcaagga gaccatcgg cgagcctct ttacagttt cgacgtaag ggctcgaca gttcagtca ggcaaaata ttggtcgtc accacacgt gttcggaga cagggcgta ttttcggca caccacgtc acctgcaac attagaaat tgcatcaat tcaactctc agatgaagg aatctaccg atgccgagt cgattttaa aaactcacc caccagaaa tagtaaagt caacttcac tgttatcgt gcctcccga aaagatcca catttacga tgacaagcg agtcgacaa gacgattct attaggacc atataatga gggctcgga cgttaattt attgtgcga agttaaggg aggccgacc tcgaccgaa ggtgacgtg gtttctcga aaacacagt gatcgatga ttcgtacga gataagatc ggatggcat agccgtcaa tcatctcac atttccaaa tgttggtcg ccaacattt acatgcaag actgatttg ccaggccag taataacaa tctggtccc acctgagac aagagtagc tgtgttaga cattaattt aaaacccca aacggtaaa tatcctaac aaaagaaaa acaagtgtc agcaggcaa acgctacga agttgagtg taggacgtc tggatcgag gccagacgc agttataac atggtggaa aggaagcag acctgtcag aaaactagc aaaaaactt ttcggaaca gaacaacca aagcttaag cattttgac gtttgtccc tgtgatcga tgacgacgg caaatactt gacctgtcg agctgaaaa cccgtccat ccctgaaag cgcgctcga ggacaagtg gagattaaa cgtccatta tactcctgt tgtaacgtt aaaaatggg ctcgacatt aaacccgga cgatatcaa agaaggtga cgacgttta tttcgaatg caatatccg ggccaaccc caaagctta caaactttc ctggttcca cgacggagt ggagatttt ccagaacgt caccgccgg agtgatcat gagcgatca aagtttggt gttgcagag cgtaacgag agccacggg aggtgctta cacgtgtat ggccaccaa cgtggaagg caaaagcac cagtaatgc cgtccaact tgtcgtgag atacgcccc cgtgtgcaa acaggaccg agaggagtt atatggcgc gttgaagca agagaccgt gactttgcg ctgtcaggt cgacgccaa tcctgccat agtgacctt ccactggac gttcaacaa ctccgggga cctgaccga agttccggc caacaggtt cacaaacga gctctcttc ctcccgact caactacac acccgtctc ggacctcga ctacggcac actcctctg ttggggtga caacgatgt gggtcacca gaaggtccc ctgcgtctt ccaagtagt catagcagg ccggccgtc gcagctgca aaattgcac cgtatcgaa ccagagctc ggattcgct ccaagtgga ctgcacaga gggcttcga tggtgggct ccctcagaa cttcttcat ggagatcct ggaactgcc agcgcttcg gtcccgact gaacgtcac cagctaccg gactcctcc gacgttttt cgccgacgg cctggatcc gggagtgag ctaccggat catgcttta cgccgtgaa tgccaaggg aaggagcga agccaccat tattgaccc cgtcacgtt caaaggtgt cgccaaatt acagggttc aacggcaag tatgccggt tagccccct aattatggg tcttctggg cactgccgg cgtgctggc ggcgggggt ctgcctcgt gctggctgc tctgtgccg gagacacta ctccaggcc gtgctcgag gcccgaagg gggcaccaa gcacgtacc tatggaagc ggttattgc cgctgacga tctcatcgt tgacggctc cgtcacggg cgtacgcac accagcgac tcccgacgc ggccattac cgccgaagc cgtgagaaa cggcacttc catagaggc cacagatcc agacattat acgaaatca atatgagcg tcgaccgac cctcggatt tatgaaagt gtacgagcc gcccgggtc cagagagga agaggacgt cgaagacga gggcgagga gtacgattt cagacacgt tgccaaaga aactcacat ccccaatca gaccgtgta ccggagtct gcagaggcc cggacgaaa ccccgcaaa ccccattcc gggcactca gaccttgac gcacaagta tcgagggcc cgaagtcat tacgacgtc gaaccgaat acaggagag ctgcatata aatgtaatt gtttttatc atgtattat attaatgta tttaaaata aataaaagc ggactttca tg
