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Gene information
General information
TC009768 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7839513..7932500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009768
    iBeetle screen iB_04694

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009768 RA
      MIVFMGLIW RILAIYLVT APQVSPIFN GLEDTSTPN 
DLDKPVPMV EVQGVLNKK KGLPCDITP KERDDAVAM 
VLWFKETIG EPLYSFDVR ARQFSQAKY WSSPHVFGD 
RAYFRHTTS PATLEIASI QLSDEGIYR CRVDFKNSP 
TRNSKVNFT VIVPPEKIH IYDDKRVDK TILLGPYNE 
GSDVNLLCE VKGGRPRPK VTWFLENTV IDDSYEIRS 
DGIAVNHLT FPNVGRQHL HARLICQAS NNNLVPPET 
RVAVLDINL KPQTVNILT KEKQVSAGK RYEVECRTS 
GSRPDAVIT WWKGSRPVR KLAKNFSEQ NNQSLSILT 
FVPVIDDDG KYLTCRAEN PSIPESALE DKWRLNVHY 
TPVVTLKMG STLNPDDIK EGDDVYFEC NIRANPKAY 
KLSWFHDGV EIFQNVTAG VIMSDQSLV LQSVTRATG 
GAYTCMATN VEGKSTSNA VQLVVRYAP VCKQDREEL 
YGALKQETV TLRCQVDAN PAIVTFHWT FNNSGDLTE 
VPANRFTNE LSSSRLNYT PVSDLDYGT LLCWGDNDV 
GHQKVPCVF QVVIAGRPS QLQNCTVSN QSSDSLQVD 
CTEGFDGGL PQNFFMEIL ELPALRSRL NVTSYRTPP 
TFFADGLDP GVSYRIMLY AVNAKGRSE ATIIDPVTF 
KGVAKLQGS TASMPVSPL IMGLLGTAG VLAAGVCLV 
LAALCRRHY SRPCSRPEG GTKHVPMEA VIAADDLIV 
DGSVTGVRT PATPDAAIT AEAVRNGTS IEATDPDII 
RNQYERRPT LGFMKVYEP PGSREEEDV EDEGEEYDF 
RHVAKETHI PNQTVYRSL QRPGRNPAN PIPGTQTLT 
HKYRGPEVI TTSNRIQES CI
    CDS sequence
    • >TC009768 RA
      atgatagtg ttcatgggg ttgatttgg agaatcctt 
gccatctat ttggtgacc gcgcctcag gtgtctccc 
atcttcaac gggctagag gatacttcc acccccaac 
gacctcgat aagcccgtt ccaatggtg gaagttcaa 
ggagtgttg aataaaaag aagggtctt ccctgtgat 
ataaccccg aaggagcgt gatgatgcc gtcgccatg 
gtgctatgg ttcaaggag accatcggc gagcctctt 
tacagtttc gacgtaagg gctcgacag ttcagtcag 
gcaaaatat tggtcgtca ccacacgtg ttcggagac 
agggcgtat tttcggcac accacgtca cctgcaaca 
ttagaaatt gcatcaatt caactctca gatgaagga 
atctaccga tgccgagtc gattttaaa aactcaccc 
accagaaat agtaaagtc aacttcact gttatcgtg 
cctcccgaa aagatccac atttacgat gacaagcga 
gtcgacaag acgattcta ttaggacca tataatgag 
ggctcggac gttaattta ttgtgcgaa gttaaggga 
ggccgacct cgaccgaag gtgacgtgg tttctcgaa 
aacacagtg atcgatgat tcgtacgag ataagatcg 
gatggcata gccgtcaat catctcaca tttccaaat 
gttggtcgc caacattta catgcaaga ctgatttgc 
caggccagt aataacaat ctggtccca cctgagaca 
agagtagct gtgttagac attaattta aaaccccaa 
acggtaaat atcctaaca aaagaaaaa caagtgtca 
gcaggcaaa cgctacgaa gttgagtgt aggacgtct 
ggatcgagg ccagacgca gttataaca tggtggaaa 
ggaagcaga cctgtcaga aaactagca aaaaacttt 
tcggaacag aacaaccaa agcttaagc attttgacg 
tttgtccct gtgatcgat gacgacggc aaatacttg 
acctgtcga gctgaaaac ccgtccatc cctgaaagc 
gcgctcgag gacaagtgg agattaaac gtccattat 
actcctgtt gtaacgtta aaaatgggc tcgacatta 
aacccggac gatatcaaa gaaggtgac gacgtttat 
ttcgaatgc aatatccgg gccaacccc aaagcttac 
aaactttcc tggttccac gacggagtg gagattttc 
cagaacgtc accgccgga gtgatcatg agcgatcaa 
agtttggtg ttgcagagc gtaacgaga gccacggga 
ggtgcttac acgtgtatg gccaccaac gtggaaggc 
aaaagcacc agtaatgcc gtccaactt gtcgtgaga 
tacgccccc gtgtgcaaa caggaccga gaggagtta 
tatggcgcg ttgaagcaa gagaccgtg actttgcgc 
tgtcaggtc gacgccaat cctgccata gtgaccttc 
cactggacg ttcaacaac tccggggac ctgaccgaa 
gttccggcc aacaggttc acaaacgag ctctcttcc 
tcccgactc aactacaca cccgtctcg gacctcgac 
tacggcaca ctcctctgt tggggtgac aacgatgtg 
ggtcaccag aaggtcccc tgcgtcttc caagtagtc 
atagcaggc cggccgtcg cagctgcaa aattgcacc 
gtatcgaac cagagctcg gattcgctc caagtggac 
tgcacagag ggcttcgat ggtgggctc cctcagaac 
ttcttcatg gagatcctg gaactgcca gcgcttcgg 
tcccgactg aacgtcacc agctaccgg actcctccg 
acgtttttc gccgacggc ctggatccg ggagtgagc 
taccggatc atgctttac gccgtgaat gccaaggga 
aggagcgaa gccaccatt attgacccc gtcacgttc 
aaaggtgtc gccaaatta cagggttca acggcaagt 
atgccggtt agcccccta attatgggt cttctgggc 
actgccggc gtgctggcg gcgggggtc tgcctcgtg 
ctggctgct ctgtgccgg agacactac tccaggccg 
tgctcgagg cccgaaggg ggcaccaag cacgtacct 
atggaagcg gttattgcc gctgacgat ctcatcgtt 
gacggctcc gtcacgggc gtacgcaca ccagcgact 
cccgacgcg gccattacc gccgaagcc gtgagaaac 
ggcacttcc atagaggcc acagatcca gacattata 
cgaaatcaa tatgagcgt cgaccgacc ctcggattt 
atgaaagtg tacgagccg cccgggtcc agagaggaa 
gaggacgtc gaagacgag ggcgaggag tacgatttc 
agacacgtt gccaaagaa actcacatc cccaatcag 
accgtgtac cggagtctg cagaggccc ggacgaaac 
cccgcaaac cccattccg ggcactcag accttgacg 
cacaagtat cgagggccc gaagtcatt acgacgtcg 
aaccgaata caggagagc tgcatataa
    mRNA sequence
    • >TC009768 RA
      gatgaccgc gaaagagat cgtttcgaa cggcgtcga 
aggcccgga aagccgcaa ggataatat gccatgccc 
gcaaacata acccctcaa aggacgaag ctttcaaac 
tttgcaact cgtgctgat tctttacga tgaattgcg 
agattttgt ggccaaacg gtgcaggat tataaagcg 
ttgtgccgg gtcctgtgt ggtgttgtg agatatttc 
agtgttgag atgtaagga aatgatagt gttcatggg 
gttgatttg gagaatcct tgccatcta tttggtgac 
cgcgcctca ggtgtctcc catcttcaa cgggctaga 
ggatacttc cacccccaa cgacctcga taagcccgt 
tccaatggt ggaagttca aggagtgtt gaataaaaa 
gaagggtct tccctgtga tataacccc gaaggagcg 
tgatgatgc cgtcgccat ggtgctatg gttcaagga 
gaccatcgg cgagcctct ttacagttt cgacgtaag 
ggctcgaca gttcagtca ggcaaaata ttggtcgtc 
accacacgt gttcggaga cagggcgta ttttcggca 
caccacgtc acctgcaac attagaaat tgcatcaat 
tcaactctc agatgaagg aatctaccg atgccgagt 
cgattttaa aaactcacc caccagaaa tagtaaagt 
caacttcac tgttatcgt gcctcccga aaagatcca 
catttacga tgacaagcg agtcgacaa gacgattct 
attaggacc atataatga gggctcgga cgttaattt 
attgtgcga agttaaggg aggccgacc tcgaccgaa 
ggtgacgtg gtttctcga aaacacagt gatcgatga 
ttcgtacga gataagatc ggatggcat agccgtcaa 
tcatctcac atttccaaa tgttggtcg ccaacattt 
acatgcaag actgatttg ccaggccag taataacaa 
tctggtccc acctgagac aagagtagc tgtgttaga 
cattaattt aaaacccca aacggtaaa tatcctaac 
aaaagaaaa acaagtgtc agcaggcaa acgctacga 
agttgagtg taggacgtc tggatcgag gccagacgc 
agttataac atggtggaa aggaagcag acctgtcag 
aaaactagc aaaaaactt ttcggaaca gaacaacca 
aagcttaag cattttgac gtttgtccc tgtgatcga 
tgacgacgg caaatactt gacctgtcg agctgaaaa 
cccgtccat ccctgaaag cgcgctcga ggacaagtg 
gagattaaa cgtccatta tactcctgt tgtaacgtt 
aaaaatggg ctcgacatt aaacccgga cgatatcaa 
agaaggtga cgacgttta tttcgaatg caatatccg 
ggccaaccc caaagctta caaactttc ctggttcca 
cgacggagt ggagatttt ccagaacgt caccgccgg 
agtgatcat gagcgatca aagtttggt gttgcagag 
cgtaacgag agccacggg aggtgctta cacgtgtat 
ggccaccaa cgtggaagg caaaagcac cagtaatgc 
cgtccaact tgtcgtgag atacgcccc cgtgtgcaa 
acaggaccg agaggagtt atatggcgc gttgaagca 
agagaccgt gactttgcg ctgtcaggt cgacgccaa 
tcctgccat agtgacctt ccactggac gttcaacaa 
ctccgggga cctgaccga agttccggc caacaggtt 
cacaaacga gctctcttc ctcccgact caactacac 
acccgtctc ggacctcga ctacggcac actcctctg 
ttggggtga caacgatgt gggtcacca gaaggtccc 
ctgcgtctt ccaagtagt catagcagg ccggccgtc 
gcagctgca aaattgcac cgtatcgaa ccagagctc 
ggattcgct ccaagtgga ctgcacaga gggcttcga 
tggtgggct ccctcagaa cttcttcat ggagatcct 
ggaactgcc agcgcttcg gtcccgact gaacgtcac 
cagctaccg gactcctcc gacgttttt cgccgacgg 
cctggatcc gggagtgag ctaccggat catgcttta 
cgccgtgaa tgccaaggg aaggagcga agccaccat 
tattgaccc cgtcacgtt caaaggtgt cgccaaatt 
acagggttc aacggcaag tatgccggt tagccccct 
aattatggg tcttctggg cactgccgg cgtgctggc 
ggcgggggt ctgcctcgt gctggctgc tctgtgccg 
gagacacta ctccaggcc gtgctcgag gcccgaagg 
gggcaccaa gcacgtacc tatggaagc ggttattgc 
cgctgacga tctcatcgt tgacggctc cgtcacggg 
cgtacgcac accagcgac tcccgacgc ggccattac 
cgccgaagc cgtgagaaa cggcacttc catagaggc 
cacagatcc agacattat acgaaatca atatgagcg 
tcgaccgac cctcggatt tatgaaagt gtacgagcc 
gcccgggtc cagagagga agaggacgt cgaagacga 
gggcgagga gtacgattt cagacacgt tgccaaaga 
aactcacat ccccaatca gaccgtgta ccggagtct 
gcagaggcc cggacgaaa ccccgcaaa ccccattcc 
gggcactca gaccttgac gcacaagta tcgagggcc 
cgaagtcat tacgacgtc gaaccgaat acaggagag 
ctgcatata aatgtaatt gtttttatc atgtattat 
attaatgta tttaaaata aataaaagc ggactttca 
tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY