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Gene information
General information
TC009748 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7231931..7233898
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009748
    iBeetle screen iB_04692

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009748 RA
      MEIENKLDE DFVFYLGFV GTYFKHIRD KDIRHHCEQ 
      WLLKLCGEP CQGIEKKRG RNIYLSQLI LCMQTGILG 
      NEFKVPVNE VDVANATQV FQLQPEGEA FQTPGWLED 
      NDADVGTAA RNAKTGRTY VATRTLPGG QGAFAYVAV 
      SLDEEEPKW LGGGEGVFD RHMEQKFRE EVPDYEMEK 
      ILARRKDPK EREKVITFY KVLLTNIED ELDEKIHAG 
      ENDTVNGLL EQLEQDMRD RGQFEPFAH LNAKDLRNE 
      LLLVLHDRI QLRINKVMK REELLDEIE KGILAKSFF 
      ETSVTPEDK FLLPPAMWE QAINKIPNK KLLEKLRDN 
      YPMILIEKF LKLLSDYKE EIAVRMHRR HENIAAQMK 
      RELRREDEK GKKLVEGAQ IACDHATEI LKAVKEAYT 
      TKAEVERRN AEKVAIPKS EHSELYDQM RAALLDTQK 
      SVEDEAARG KVLAAQIGE INEQTEMCL KVTEENVRK 
      IEEKNMEIM KNIKRLNAA IDNQQKRIE MVQKVGAKK 
      GNQLEFFF
    CDS sequence
    • >TC009748 RA
      atggaaatc gagaacaaa ttagacgag gacttcgtc 
      ttctacctc ggcttcgtc ggtacttac ttcaaacat 
      atccgcgat aaagacatt cgtcaccac tgcgaacaa 
      tggttgcta aaactctgc ggggagcct tgccaagga 
      attgaaaag aaacgaggc cgcaatatc tacctctca 
      caactcatt ctatgcatg caaactgga attttgggc 
      aatgaattt aaagttccg gtcaacgaa gtcgatgta 
      gcgaatgcg acccaggtg ttccagctg cagcccgaa 
      ggagaagca tttcagacc ccaggatgg ttggaggat 
      aacgatgct gatgtaggt actgctgcc aggaatgca 
      aaaactggg cggacttac gtggctacg cgtacattg 
      ccgggagga caaggggct tttgcgtac gttgccgtc 
      tccttagac gaggaagaa cccaagtgg ttgggaggc 
      ggggaaggt gtttttgac cggcatatg gaacagaag 
      ttcagggag gaggtgccc gattatgaa atggaaaag 
      attctagca aggaggaaa gatcctaaa gagcgggag 
      aaggttatc accttctat aaagtcctg ttgacaaat 
      attgaagat gagttggac gagaagata catgcaggt 
      gaaaatgac actgttaat ggtcttttg gagcaactg 
      gaacaagat atgagggat cgtggccag tttgaacca 
      ttcgcacac ttgaatgct aaagattta agaaacgaa 
      cttcttcta gtgctacac gatcgcatt cagctaagg 
      attaataaa gtgatgaaa cgtgaggaa cttctcgat 
      gaaattgag aaaggcatt cttgcgaaa tcattcttt 
      gaaacctct gtaacgcca gaagacaag ttcttgtta 
      cccccggca atgtgggag caagctatc aataaaatc 
      cccaacaaa aaactgctt gagaaatta agggataac 
      tatccgatg attctaata gagaaattc ttgaagcta 
      ctttctgat tataaagaa gaaatagct gtgagaatg 
      caccgtcga catgaaaac atcgccgcg cagatgaag 
      cgggagtta agacgtgaa gacgagaag gggaagaag 
      cttgtcgaa ggtgcccaa atcgcctgc gaccacgct 
      actgagatt ctcaaggct gtcaaagaa gcctatacc 
      actaaggcc gaagtcgag aggagaaat gcagagaag 
      gttgccatt ccaaaatcg gagcattct gagctttat 
      gatcaaatg agggccgct ttgcttgac actcagaag 
      tctgtcgag gatgaagcg gccagagga aaagtgttg 
      gctgctcaa attggagaa atcaacgaa cagactgaa 
      atgtgtttg aaagtaacg gaagaaaat gtcaggaag 
      attgaagag aagaatatg gaaataatg aaaaatatc 
      aagagactg aatgccgca attgacaat cagcagaag 
      aggatcgaa atggtgcag aaggtgggg gcgaagaag 
      ggaaatcag cttgaattt ttcttttaa
    mRNA sequence
    • >TC009748 RA
      tcaaacctt taacaccct tcaaactct cgtcaaaat 
      tctagcgca atggaaatc gagaacaaa ttagacgag 
      gacttcgtc ttctacctc ggcttcgtc ggtacttac 
      ttcaaacat atccgcgat aaagacatt cgtcaccac 
      tgcgaacaa tggttgcta aaactctgc ggggagcct 
      tgccaagga attgaaaag aaacgaggc cgcaatatc 
      tacctctca caactcatt ctatgcatg caaactgga 
      attttgggc aatgaattt aaagttccg gtcaacgaa 
      gtcgatgta gcgaatgcg acccaggtg ttccagctg 
      cagcccgaa ggagaagca tttcagacc ccaggatgg 
      ttggaggat aacgatgct gatgtaggt actgctgcc 
      aggaatgca aaaactggg cggacttac gtggctacg 
      cgtacattg ccgggagga caaggggct tttgcgtac 
      gttgccgtc tccttagac gaggaagaa cccaagtgg 
      ttgggaggc ggggaaggt gtttttgac cggcatatg 
      gaacagaag ttcagggag gaggtgccc gattatgaa 
      atggaaaag attctagca aggaggaaa gatcctaaa 
      gagcgggag aaggttatc accttctat aaagtcctg 
      ttgacaaat attgaagat gagttggac gagaagata 
      catgcaggt gaaaatgac actgttaat ggtcttttg 
      gagcaactg gaacaagat atgagggat cgtggccag 
      tttgaacca ttcgcacac ttgaatgct aaagattta 
      agaaacgaa cttcttcta gtgctacac gatcgcatt 
      cagctaagg attaataaa gtgatgaaa cgtgaggaa 
      cttctcgat gaaattgag aaaggcatt cttgcgaaa 
      tcattcttt gaaacctct gtaacgcca gaagacaag 
      ttcttgtta cccccggca atgtgggag caagctatc 
      aataaaatc cccaacaaa aaactgctt gagaaatta 
      agggataac tatccgatg attctaata gagaaattc 
      ttgaagcta ctttctgat tataaagaa gaaatagct 
      gtgagaatg caccgtcga catgaaaac atcgccgcg 
      cagatgaag cgggagtta agacgtgaa gacgagaag 
      gggaagaag cttgtcgaa ggtgcccaa atcgcctgc 
      gaccacgct actgagatt ctcaaggct gtcaaagaa 
      gcctatacc actaaggcc gaagtcgag aggagaaat 
      gcagagaag gttgccatt ccaaaatcg gagcattct 
      gagctttat gatcaaatg agggccgct ttgcttgac 
      actcagaag tctgtcgag gatgaagcg gccagagga 
      aaagtgttg gctgctcaa attggagaa atcaacgaa 
      cagactgaa atgtgtttg aaagtaacg gaagaaaat 
      gtcaggaag attgaagag aagaatatg gaaataatg 
      aaaaatatc aagagactg aatgccgca attgacaat 
      cagcagaag aggatcgaa atggtgcag aaggtgggg 
      gcgaagaag ggaaatcag cttgaattt ttcttttaa 
      cgttttgaa aaacggaac acataataa atacttttg 
      aaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY