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Gene information
General information
TC009742 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7167211..7169935
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009742
    iBeetle screen iB_04691

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009742 RA
      MKPHPQIEH TPEVSSRRR ALFPDSSPC DDDLGCISP 
      LDFDEDCAK NVFSHDTCV SDYDIIGLV EKGHTVHTP 
      NNTNITTKV FTPSTTNES PTQKLKTPH DAYTNTIFP 
      KLIRSKTQV DTAPNQDCK NPKIRTALF PDKDMSLPS 
      RKFYPKSDN EVLPRPFAL TKRPNPTKG RKRNPHFLC 
      NRRSKFSRS GQINAGVRH KIKKRKIRK ISKIAALEA 
      AINIIENSP LNTVLDSTE SIEVCPESI QVAPEEPPR 
      DPTPSPEPD PTKKFFKSC RHKAVVTVN PNIKLEMNN 
      GRISLIQNK NPLKRPRVQ EGISPKRPK LDFEPEKPL 
      IPDEEISNI IDSLEDKEN EGKNPDLLM SPTSQMCNM 
      TSGLALNSP KKARDLSSV LESMPSNDK KLFPVFYPG 
      KTALPTPTK ENKVLNVAK KFRPISKDQ MLLDAGQKR 
      WGVTQCTEC KFIYHMGDP GDEIMHLNY HNAGHIFRY 
      TGWKNERVV SSDLRNGKV IQIVPGDSK IWWKKVKEV 
      MEVINRDLG YFDIEFNTA NSQIFFYVK SKVIIGALV 
      AEVKTTGHK LLTTHQDQV DLCSEESYP VKCGVSRIW 
      VARNHRQKG VATALMDAL KRNFVFGAI LMNEDIALS 
      SPTEDGKIF GAKYFKTPN FFIYFV
    CDS sequence
    • >TC009742 RA
      atgaagcct catccgcaa atcgagcac actccagag 
      gtttccagc agaagaaga gccctcttt ccagacagc 
      tccccgtgc gacgacgat ctagggtgc ataagtccg 
      ctggacttc gacgaggac tgtgccaaa aacgtcttt 
      tcccacgac acatgtgtc agcgattat gacatcatt 
      ggtctcgtg gaaaagggc cacacagtc cacacaccg 
      aacaacacc aacatcact actaaagtc ttcacgcct 
      tccaccacc aatgaatcg ccaacacag aaactcaaa 
      acgccacat gatgcatac accaacaca atttttcct 
      aaattaata cgtagtaaa acgcaagtt gacacagcc 
      cctaaccaa gactgcaaa aatccgaaa atacgtacc 
      gctctcttt cctgacaaa gacatgtct ctcccttcc 
      agaaagttt taccccaag tccgataac gaggtcctg 
      ccacgaccc tttgccctc accaagaga cccaacccc 
      acaaagggg cgcaaaaga aacccccat ttcttgtgc 
      aacaggagg agcaagttc agtcgatca gggcaaatc 
      aacgcggga gtcagacac aagataaaa aagcgaaaa 
      atccgcaaa atcagcaaa attgcagcc ctcgaggcc 
      gcaattaac ataatcgaa aactctcct ctcaataca 
      gtccttgat tcaactgag tcgatcgaa gtgtgccct 
      gaatccatc caggtcgcc cccgaagag ccccctcgc 
      gacccaact cccagtccc gaacccgac cccacaaaa 
      aaattcttc aaatcttgc cgtcacaaa gccgtagtc 
      acagtaaac cccaacata aaactcgaa atgaacaac 
      ggacggatt agtttaatt cagaacaag aacccgctg 
      aaacggccg agagtgcaa gagggaatc agccccaaa 
      cgtccaaaa ttggacttc gagcctgag aaaccccta 
      attcccgat gaggaaatt tcgaatatt atcgattct 
      ctggaagat aaagaaaac gaggggaaa aatccggac 
      ttgttgatg tcccccacg tctcagatg tgtaacatg 
      acctcggga ctggctcta aacagtccg aaaaaagca 
      cgggacttg agctcggtt ctggagtcg atgccgtcg 
      aatgataag aaattattc ccggttttc tatccaggg 
      aaaactgcc ttaccgacc ccaaccaag gagaataag 
      gtgttgaac gtagcgaag aagtttagg ccgatttcc 
      aaggaccag atgttgcta gatgcggga caaaagaga 
      tggggggtc acccagtgc accgagtgt aaatttatt 
      tatcatatg ggagacccg ggtgatgaa atcatgcat 
      ctgaattat cacaacgcc ggccatatt ttccgatat 
      actggttgg aaaaacgaa cgcgtcgtc tccagcgac 
      ctacgcaac ggtaaagtc atccaaatt gtaccgggt 
      gactcaaaa atctggtgg aagaaagtc aaagaggtg 
      atggaagtg atcaaccgc gatttgggg tactttgat 
      attgaattc aacacagct aactcacag atatttttc 
      tacgtcaaa agtaaagtt ataatcggg gctttggtg 
      gcggaagtc aaaacaacc ggtcataaa ttactaaca 
      acccaccaa gaccaagtc gacttatgt tcagaggag 
      tcctaccct gtgaaatgt ggggtttcc cggatttgg 
      gtggcccgc aaccatcga caaaagggc gtagcaacg 
      gcccttatg gacgcactt aagagaaac ttcgtattt 
      ggcgcgatt ttaatgaac gaagacatt gccttgagc 
      tccccaact gaagacggg aaaattttc ggggcaaaa 
      tatttcaaa acgccgaac ttttttatt tattttgta 
      taa
    mRNA sequence
    • >TC009742 RA
      tgggaaata aaataattt taaccactg ttctaacac 
      tgaaactta ctgaccatt tttattttt ttagttatc 
      tctccacta ttgctggtc tttgatttt ttgttgcaa 
      accgttttt agaaaaacc tcccgcgca tgcgtctgt 
      gtcaccctg atcccggca gccaatcat cttcctgtt 
      tttccggcg ggaacggct tttcatgtc ccctaatcc 
      gttaagttt ggttagaaa aacggtttg aggtgcgtg 
      aaccgtaaa caaaccccg ccattcgtg cagccgtca 
      cgtgtgatt gttatgccc cttcagagc ggatgtttc 
      ccgccagtt ggtcagacc caatgtgat gaagcctca 
      tccgcaaat cgagcacac tccagaggt ttccagcag 
      aagaagagc cctctttcc agacagctc cccgtgcga 
      cgacgatct agggtgcat aagtccgct ggacttcga 
      cgaggactg tgccaaaaa cgtcttttc ccacgacac 
      atgtgtcag cgattatga catcattgg tctcgtgga 
      aaagggcca cacagtcca cacaccgaa caacaccaa 
      catcactac taaagtctt cacgccttc caccaccaa 
      tgaatcgcc aacacagaa actcaaaac gccacatga 
      tgcatacac caacacaat ttttcctaa attaatacg 
      tagtaaaac gcaagttga cacagcccc taaccaaga 
      ctgcaaaaa tccgaaaat acgtaccgc tctctttcc 
      tgacaaaga catgtctct cccttccag aaagtttta 
      ccccaagtc cgataacga ggtcctgcc acgaccctt 
      tgccctcac caagagacc caaccccac aaaggggcg 
      caaaagaaa cccccattt cttgtgcaa caggaggag 
      caagttcag tcgatcagg gcaaatcaa cgcgggagt 
      cagacacaa gataaaaaa gcgaaaaat ccgcaaaat 
      cagcaaaat tgcagccct cgaggccgc aattaacat 
      aatcgaaaa ctctcctct caatacagt ccttgattc 
      aactgagtc gatcgaagt gtgccctga atccatcca 
      ggtcgcccc cgaagagcc ccctcgcga cccaactcc 
      cagtcccga acccgaccc cacaaaaaa attcttcaa 
      atcttgccg tcacaaagc cgtagtcac agtaaaccc 
      caacataaa actcgaaat gaacaacgg acggattag 
      tttaattca gaacaagaa cccgctgaa acggccgag 
      agtgcaaga gggaatcag ccccaaacg tccaaaatt 
      ggacttcga gcctgagaa acccctaat tcccgatga 
      ggaaatttc gaatattat cgattctct ggaagataa 
      agaaaacga ggggaaaaa tccggactt gttgatgtc 
      ccccacgtc tcagatgtg taacatgac ctcgggact 
      ggctctaaa cagtccgaa aaaagcacg ggacttgag 
      ctcggttct ggagtcgat gccgtcgaa tgataagaa 
      attattccc ggttttcta tccagggaa aactgcctt 
      accgacccc aaccaagga gaataaggt gttgaacgt 
      agcgaagaa gtttaggcc gatttccaa ggaccagat 
      gttgctaga tgcgggaca aaagagatg gggggtcac 
      ccagtgcac cgagtgtaa atttattta tcatatggg 
      agacccggg tgatgaaat catgcatct gaattatca 
      caacgccgg ccatatttt ccgatatac tggttggaa 
      aaacgaacg cgtcgtctc cagcgacct acgcaacgg 
      taaagtcat ccaaattgt accgggtga ctcaaaaat 
      ctggtggaa gaaagtcaa agaggtgat ggaagtgat 
      caaccgcga tttggggta ctttgatat tgaattcaa 
      cacagctaa ctcacagat atttttcta cgtcaaaag 
      taaagttat aatcggggc tttggtggc ggaagtcaa 
      aacaaccgg tcataaatt actaacaac ccaccaaga 
      ccaagtcga cttatgttc agaggagtc ctaccctgt 
      gaaatgtgg ggtttcccg gatttgggt ggcccgcaa 
      ccatcgaca aaagggcgt agcaacggc ccttatgga 
      cgcacttaa gagaaactt cgtatttgg cgcgatttt 
      aatgaacga agacattgc cttgagctc cccaactga 
      agacgggaa aattttcgg ggcaaaata tttcaaaac 
      gccgaactt ttttattta ttttgtata attatttaa 
      tgccatata ccttttttg tatacctgc atgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY