Status
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Gene information
General information
TC033668
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009736,TC009738,TC009735
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6600432..6647583
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033668
    iBeetle screen iB_04690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033668 RA
      MSIKILLMW TILFTDPTT SVFAAECSP PLSCLLITK 
      CKPLMTAIK TTQNSPAII KFLKAQICS HNNKQEPKV 
      CCPDNPLNN KKQLSESPC KPSFRCVSL SGCGPLMYL 
      VNLEKPIPA GIIKFIKSQ QCGTGKTYP KVCCKLEAI 
      DSDEKSTQS VAEKAFSKP IPQKVKAEI MNKLFNDEF 
      FDFTVTAPT VGAVLFETV QFIAGLGAS NYKCVSVNN 
      RGMKRRGAG LFASVALWT LLLHLTDAD WWQLGLPVS 
      MTNSVDLTP EAHKERCSK LEYLVESQK QLCSQYDRI 
      LPVIGNGAR LAMDECQHQ FRNSRWNCT AFPEKNVTF 
      GNVITIRSR EAAYLSAVS AASVAFAVT RACSKGDLT 
      DCSCDTRMR QKKSHKWKW GGCSEDIKY GEKFSRDFL 
      DSKEDDKTA DGLMNLHNN EAGRRSVKS RMVRTCKCH 
      GVSGSCSMQ ICWRRLPPF RKVGDALFQ RYEGASHVK 
      FVERKWRKK LKAISADLK KPNKTDLVY LDDSPDYCE 
      KNETLSILG THGRICNRT SQGIDGCRL LCCGRGYQT 
      RVREVEEKC KCHFVWCCN VVCDICRYR REEHVCN
    CDS sequence
    • >TC033668 RA
      atgagtata aaaatactg ttaatgtgg accattctt 
      ttcaccgat ccaacaact tcagtattt gcagctgaa 
      tgctctcca ccactatca tgccttctc ataacaaaa 
      tgcaaacct ctcatgact gcaataaaa accacacaa 
      aattctccc gcaataata aaatttctg aaagctcaa 
      atctgtagt cacaacaac aaacaagag ccaaaagtc 
      tgttgtccc gataatcct ctcaacaat aaaaaacaa 
      ttaagtgaa tcaccgtgc aaaccctct tttcgatgc 
      gtcagtctg tctggatgc gggcctttg atgtatttg 
      gtaaatttg gaaaaaccg atccccgca gggatcata 
      aaatttatc aagtctcag caatgcggg acaggtaaa 
      acctacccc aaggtgtgt tgtaaattg gaggcaata 
      gattcggat gagaaatcc acgcaaagt gtagcggaa 
      aaggctttc agcaagccc ataccccag aaggtcaag 
      gctgagatc atgaacaaa ctttttaat gatgaattt 
      tttgatttc acggtgacc gcccccact gtcggcgcc 
      gtgttgttc gaaaccgtt cagttcata gctggttta 
      ggtgcaagc aactacaag tgcgtctct gtgaataac 
      cgcggcatg aagcgtcgc ggcgcggga ctctttgct 
      tccgtcgct ttgtggacg cttctactg catctcacg 
      gatgccgac tggtggcag ctcggattg ccagtttcc 
      atgaccaat tcggtagac ttgacaccg gaggcccat 
      aaagaacgc tgcagcaaa ttagagtac ctggttgag 
      agccaaaaa caactgtgt tcacaatac gataggata 
      ttaccggtt ataggaaat ggcgccagg ttggcgatg 
      gacgaatgc caacatcaa tttaggaat agtagatgg 
      aattgtaca gcgtttccc gaaaaaaat gtaacgttt 
      ggaaatgtg atcacaatt agaagtaga gaagcagcg 
      tacttatca gcagtatca gcagcaagc gtagctttt 
      gcagtgacg cgagcgtgt tcgaaaggc gatttaaca 
      gactgttcc tgcgacacg agaatgcgc cagaagaaa 
      tcgcacaaa tggaagtgg ggcggctgt tccgaagac 
      atcaaatac ggagagaaa ttcagccga gacttttta 
      gattccaaa gaggatgac aaaacagcg gacggactt 
      atgaatctt cacaacaat gaagcagga agaagaagt 
      gtcaaatcg cgaatggtc cgtacttgt aaatgccac 
      ggagtgtct ggctcctgc agtatgcag atttgctgg 
      aggcgcctc ccacccttt cggaaggtg ggcgacgcc 
      ctgttccaa agatacgaa ggcgcctct catgtgaaa 
      ttcgtcgag aggaagtgg cggaagaaa ctgaaagcg 
      atcagcgcc gatctgaag aaacccaac aaaacggat 
      ctggtgtat ttagatgat tctccggat tattgcgag 
      aaaaatgaa acgctcagt attttgggg acccatggc 
      agaatatgc aatcggacc agtcaaggg attgacggt 
      tgcagattg ttgtgctgc ggcagaggc taccagacg 
      cgggttcgc gaagtggaa gagaaatgc aagtgccac 
      ttcgtgtgg tgctgcaac gtcgtctgt gacatttgc 
      aggtatagg agagaagaa cacgtttgc aactag
    mRNA sequence
    • >TC033668 RA
      gaatgaaac tcgaataaa tctgaaaac tgtgtcaaa 
      atgagtata aaaatactg ttaatgtgg accattctt 
      ttcaccgat ccaacaact tcagtattt gcagctgaa 
      tgctctcca ccactatca tgccttctc ataacaaaa 
      tgcaaacct ctcatgact gcaataaaa accacacaa 
      aattctccc gcaataata aaatttctg aaagctcaa 
      atctgtagt cacaacaac aaacaagag ccaaaagtc 
      tgttgtccc gataatcct ctcaacaat aaaaaacaa 
      ttaagtgaa tcaccgtgc aaaccctct tttcgatgc 
      gtcagtctg tctggatgc gggcctttg atgtatttg 
      gtaaatttg gaaaaaccg atccccgca gggatcata 
      aaatttatc aagtctcag caatgcggg acaggtaaa 
      acctacccc aaggtgtgt tgtaaattg gaggcaata 
      gattcggat gagaaatcc acgcaaagt gtagcggaa 
      aaggctttc agcaagccc ataccccag aaggtcaag 
      gctgagatc atgaacaaa ctttttaat gatgaattt 
      tttgatttc acggtgacc gcccccact gtcggcgcc 
      gtgttgttc gaaaccgtt cagttcata gctggttta 
      ggtgcaagc aactacaag tgcgtctct gtgaataac 
      cgcggcatg aagcgtcgc ggcgcggga ctctttgct 
      tccgtcgct ttgtggacg cttctactg catctcacg 
      gatgccgac tggtggcag ctcggattg ccagtttcc 
      atgaccaat tcggtagac ttgacaccg gaggcccat 
      aaagaacgc tgcagcaaa ttagagtac ctggttgag 
      agccaaaaa caactgtgt tcacaatac gataggata 
      ttaccggtt ataggaaat ggcgccagg ttggcgatg 
      gacgaatgc caacatcaa tttaggaat agtagatgg 
      aattgtaca gcgtttccc gaaaaaaat gtaacgttt 
      ggaaatgtg atcacaatt agaagtaga gaagcagcg 
      tacttatca gcagtatca gcagcaagc gtagctttt 
      gcagtgacg cgagcgtgt tcgaaaggc gatttaaca 
      gactgttcc tgcgacacg agaatgcgc cagaagaaa 
      tcgcacaaa tggaagtgg ggcggctgt tccgaagac 
      atcaaatac ggagagaaa ttcagccga gacttttta 
      gattccaaa gaggatgac aaaacagcg gacggactt 
      atgaatctt cacaacaat gaagcagga agaagaagt 
      gtcaaatcg cgaatggtc cgtacttgt aaatgccac 
      ggagtgtct ggctcctgc agtatgcag atttgctgg 
      aggcgcctc ccacccttt cggaaggtg ggcgacgcc 
      ctgttccaa agatacgaa ggcgcctct catgtgaaa 
      ttcgtcgag aggaagtgg cggaagaaa ctgaaagcg 
      atcagcgcc gatctgaag aaacccaac aaaacggat 
      ctggtgtat ttagatgat tctccggat tattgcgag 
      aaaaatgaa acgctcagt attttgggg acccatggc 
      agaatatgc aatcggacc agtcaaggg attgacggt 
      tgcagattg ttgtgctgc ggcagaggc taccagacg 
      cgggttcgc gaagtggaa gagaaatgc aagtgccac 
      ttcgtgtgg tgctgcaac gtcgtctgt gacatttgc 
      aggtatagg agagaagaa cacgtttgc aactagact 
      atgaactga cgatcgact gaagatacc gaagactac 
      gatattata ctcagaaaa cgtcctcat cgtattaac 
      aaagagaga actcttcat tttccattt ttcaaactg 
      tagataatt gtaatgact cgttttctc aaaatttgt 
      ctgtttaga gttagatgg tagacgttt tatacaatt 
      ttaggatcc aaaattgta cacattatc tgttgcaat 
      tatctcaaa ctcacgact gcttcgacc attctcatc 
      cagtaaatg tatcatcac gttgtatat ttatttatg 
      tgtttgcac ctagtacat aaataattt taataatgt 
      ggaatgtat tgagattta tatagatct gtacataga 
      tttttaata tatttatta ggtacttaa ctgtagtag 
      agctaagtt cattgtaca tagtaacat agttaattt 
      aaacaaaac gtaatttat gtcgtgttc aatcaaaat 
      attaaaact agctcatct atgaactac tctggtgat 
      aaaatttat ttttccagt gtattacat gtgccataa 
      aatccattc gatccgacg atttatctt agaaactct 
      ttcgtctct actgtgttt ataaaaatc gacaccgta 
      ataaattat tattaataa actctttgt gtacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY