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Gene information
General information
TC009702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4646159..4647346
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009702
      iBeetle screen iB_04685

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009702 RA
        MKPCVVFFL LLSFVCLLE AVPHKRETF CRDNRCLTC 
        IGRYCTTTC EGEDCKKCP LGDCCDGSK CNICRGARC 
        CETSECNNC VNECLKKCI GKATCHYDC FQECLPNGG 
        GSQQENKTY YGSPLVNNH NYFPVNVTT IINITNSLY 
        NDNAIYNPI FVNTTNVNN YTGDQKTYH HKQVILGGG 
        NNEKPEGKP VLLDLPEET NSTQNCCEV VHPENCQTV 
        PRESSRLCF TGKHNECSQ ICIGKHVHI VEDKEQSHQ 
        CVSIAHKPY FFCGNYVVE NCDGCYECK NAEDENEVK 
        CDEQESCPE KCKSSMIDE QFYNRLYRK D
      CDS sequence
      • >TC009702 RA
        atgaaaccc tgtgttgta tttttcctc ttgctctca 
        tttgtgtgt ttgctggag gcagttcca cacaaaaga 
        gaaactttc tgtcgcgac aaccgatgt ttgacttgc 
        atcggccgt tattgcacc acaacgtgc gaaggagaa 
        gactgcaaa aaatgtcct ttgggtgac tgttgcgac 
        ggttccaag tgcaatatt tgcagaggc gccagatgt 
        tgcgaaacg tccgaatgc aataattgc gttaatgaa 
        tgtttgaag aagtgcata gggaaggcc acttgtcac 
        tatgattgc ttccaagag tgtcttcct aacggaggt 
        ggttcgcaa caggagaat aagacctac tatggatca 
        ccgttagta aataaccac aactatttc ccggttaat 
        gttaccacg attatcaat ataaccaac tcgctttac 
        aatgacaat gctatatac aacccaatt ttcgtaaac 
        accacaaac gttaacaac tatacgggg gatcaaaag 
        acctatcat cacaaacaa gtgattctt ggtggcggc 
        aacaatgaa aagccagag ggaaagccc gtacttctg 
        gaccttcct gaggaaacc aacagcaca caaaattgt 
        tgcgaagtg gtacatccg gaaaactgt caaacggta 
        ccacgagaa agcagtcgt ttgtgtttt acaggaaaa 
        cacaacgag tgttcacag atctgtatc ggaaaacat 
        gttcacata gttgaagac aaggaacaa agccatcag 
        tgtgtgtcg attgcacac aaaccgtat ttcttttgc 
        ggcaattat gttgttgaa aattgcgat ggctgctac 
        gagtgcaag aatgcggaa gatgaaaat gaggtgaaa 
        tgtgatgaa caagagtct tgccccgaa aagtgcaag 
        agctccatg atcgatgaa caattctac aatcgattg 
        tacagaaag gactga
      mRNA sequence
      • >TC009702 RA
        ccttccaag ataacgttt attgacaaa tttcctccc 
        aaattttat cgcatttgc attctttaa ctgcaaccc 
        ggccagaga gtgctaatt tttgcaaca gtttccaaa 
        tgaaaccct gtgttgtat ttttcctct tgctctcat 
        ttgtgtgtt tgctggagg cagttccac acaaaagag 
        aaactttct gtcgcgaca accgatgtt tgacttgca 
        tcggccgtt attgcacca caacgtgcg aaggagaag 
        actgcaaaa aatgtcctt tgggtgact gttgcgacg 
        gttccaagt gcaatattt gcagaggcg ccagatgtt 
        gcgaaacgt ccgaatgca ataattgcg ttaatgaat 
        gtttgaaga agtgcatag ggaaggcca cttgtcact 
        atgattgct tccaagagt gtcttccta acggaggtg 
        gttcgcaac aggagaata agacctact atggatcac 
        cgttagtaa ataaccaca actatttcc cggttaatg 
        ttaccacga ttatcaata taaccaact cgctttaca 
        atgacaatg ctatataca acccaattt tcgtaaaca 
        ccacaaacg ttaacaact atacggggg atcaaaaga 
        cctatcatc acaaacaag tgattcttg gtggcggca 
        acaatgaaa agccagagg gaaagcccg tacttctgg 
        accttcctg aggaaacca acagcacac aaaattgtt 
        gcgaagtgg tacatccgg aaaactgtc aaacggtac 
        cacgagaaa gcagtcgtt tgtgtttta caggaaaac 
        acaacgagt gttcacaga tctgtatcg gaaaacatg 
        ttcacatag ttgaagaca aggaacaaa gccatcagt 
        gtgtgtcga ttgcacaca aaccgtatt tcttttgcg 
        gcaattatg ttgttgaaa attgcgatg gctgctacg 
        agtgcaaga atgcggaag atgaaaatg aggtgaaat 
        gtgatgaac aagagtctt gccccgaaa agtgcaaga 
        gctccatga tcgatgaac aattctaca atcgattgt 
        acagaaagg actgatttt tcagttgta ataaagtag 
        tcaaaactg t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY