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Gene information
General information
TC009694 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4625139..4627208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009694
    iBeetle screen iB_04683

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009694 RA
      MDSPVVVLK EGRVKGSIG RDYFNNPYY YFLGIPYGK 
      APIGELRFK LPSDGNNNL KPVMVWIHG GGFLYDSNR 
      REMYGPEYL ITEDVVIVS VNYRLGVFG FLSLENPAL 
      EVPGNAGMK DMVLALKWV QNNITSFSG DPNNVTVFG 
      ESAGSAAVH YLYLSPKTK GLFHRAICQ SGCALNSWA 
      IGCTNGTII ADYLNFTER DEGKILEYL KSLPPQRIV 
      KALHRYDDT FIASRIRPF GPVVEKYAT ESAFLTQEP 
      LQIIKSGTF NKVPLIIGN NSREGILAE VARKVFGEN 
      VIGTSFEDE IYFYLKLER GSGESKQIA QKIKDFYFN 
      GEDRSEHNN EKFYLLKTD NMFLHGTYK TVNYQKQAT 
      DAPIYFYEM TLDHPFSLF KSMCTPRFF YPTMFFYFL 
      AYNVGPGRL NQIFLRLAN LMPRKTLVG ASHADDLSY 
      IFRPILGEK VRSGSEEEI YMRRFIRLW ANFARTGNP 
      TPGTSDPLL NGVEWKPVT KDQNCILDI GTRLEMKNY 
      PHVERMQFW DSIYSY
    CDS sequence
    • >TC009694 RA
      atggacagt cctgttgtg gtacttaaa gaaggtcga 
      gtcaaggga agtatcggc agggattat ttcaataat 
      ccctactac tatttcttg ggcattcct tacggaaaa 
      gcgccaatt ggggaattg cgattcaag ctccccagc 
      gatggtaac aacaacctc aaaccagtc atggtctgg 
      atccacggt ggtggcttc ctgtacgac tcaaaccgc 
      cgcgaaatg tacggtccc gaatatttg ataacggaa 
      gacgtggtt atagtttcc gtaaattac cgactcggc 
      gtgttcggc ttcctcagt ttggaaaac ccagcactt 
      gaagtgcca ggaaacgcg ggaatgaag gacatggtc 
      ctggccctc aaatgggtc cagaacaac atcacgagt 
      ttcagtggc gatccaaac aatgtcacg gtttttggc 
      gaaagcgct ggttcagcc gccgtccac tatttatac 
      ctctcgcca aaaactaaa ggactgttt cacagagcg 
      atctgccag agtggctgt gctttgaac agctgggca 
      attgggtgc actaacggt accatcatt gccgattat 
      ctcaatttt acggaacgg gatgaagga aaaatttta 
      gagtatttg aaaagtttg ccgccccaa agaatcgtc 
      aaagctttg catcgatat gacgacact tttattgca 
      agtcggatt cgaccgttt ggcccagtt gttgaaaaa 
      tacgccacg gaatcggcg ttcctaacc caggagccg 
      ttgcaaatc atcaagtcg gggacattc aacaaggtg 
      cccttaatc atagggaat aattctcgg gaagggatc 
      cttgccgaa gtggctcgg aaagtcttc ggcgaaaac 
      gtgatcggt acgagtttc gaagacgaa atttacttc 
      tacctcaag ctggagaga ggttcggga gagtcgaaa 
      caaattgcc caaaaaatc aaggatttt tatttcaat 
      ggagaggac cggtcggaa cacaacaac gaaaaattt 
      tatttgtta aaaaccgac aacatgttc ctccacggg 
      acgtacaaa accgtcaac taccagaaa caggcaacc 
      gacgcgccc atttatttc tacgaaatg accctagac 
      caccctttc agcctgttc aagagcatg tgcaccccc 
      agatttttc taccccacc atgtttttc tattttctg 
      gcgtacaat gtgggcccc ggtcgcctc aaccagata 
      ttcctgcgg ttggccaac ctcatgccg cgcaaaacc 
      ttggttggg gcgtcacat gcggacgac ttgagctac 
      attttccgg ccgattttg ggcgaaaaa gtgcgctcc 
      gggagcgag gaggaaatt tacatgagg cggtttatc 
      cgactttgg gccaatttt gcaagaact gggaacccc 
      accccgggc acaagcgac ccccttttg aatggtgtt 
      gagtggaaa ccggtgact aaagaccaa aactgcatc 
      ctggacatc ggcacccga ctcgagatg aaaaattac 
      ccacacgtc gaaaggatg cagttttgg gacagcatt 
      tacagttac tga
    mRNA sequence
    • >TC009694 RA
      agtaattaa aaaccagcg ttaggaaaa cgtactcaa 
      tttttaaca caaccaaac cgacagaaa ttcaagtgc 
      cccaccaag tcgttaaac aatttgtgt ttccgctgc 
      ggttagttt atagtaacg tgatggaca gtcctgttg 
      tggtactta aagaaggtc gagtcaagg gaagtatcg 
      gcagggatt atttcaata atccctact actatttct 
      tgggcattc cttacggaa aagcgccaa ttggggaat 
      tgcgattca agctcccca gcgatggta acaacaacc 
      tcaaaccag tcatggtct ggatccacg gtggtggct 
      tcctgtacg actcaaacc gccgcgaaa tgtacggtc 
      ccgaatatt tgataacgg aagacgtgg ttatagttt 
      ccgtaaatt accgactcg gcgtgttcg gcttcctca 
      gtttggaaa acccagcac ttgaagtgc caggaaacg 
      cgggaatga aggacatgg tcctggccc tcaaatggg 
      tccagaaca acatcacga gtttcagtg gcgatccaa 
      acaatgtca cggtttttg gcgaaagcg ctggttcag 
      ccgccgtcc actatttat acctctcgc caaaaacta 
      aaggactgt ttcacagag cgatctgcc agagtggct 
      gtgctttga acagctggg caattgggt gcactaacg 
      gtaccatca ttgccgatt atctcaatt ttacggaac 
      gggatgaag gaaaaattt tagagtatt tgaaaagtt 
      tgccgcccc aaagaatcg tcaaagctt tgcatcgat 
      atgacgaca cttttattg caagtcgga ttcgaccgt 
      ttggcccag ttgttgaaa aatacgcca cggaatcgg 
      cgttcctaa cccaggagc cgttgcaaa tcatcaagt 
      cggggacat tcaacaagg tgcccttaa tcataggga 
      ataattctc gggaaggga tccttgccg aagtggctc 
      ggaaagtct tcggcgaaa acgtgatcg gtacgagtt 
      tcgaagacg aaatttact tctacctca agctggaga 
      gaggttcgg gagagtcga aacaaattg cccaaaaaa 
      tcaaggatt tttatttca atggagagg accggtcgg 
      aacacaaca acgaaaaat tttatttgt taaaaaccg 
      acaacatgt tcctccacg ggacgtaca aaaccgtca 
      actaccaga aacaggcaa ccgacgcgc ccatttatt 
      tctacgaaa tgaccctag accaccctt tcagcctgt 
      tcaagagca tgtgcaccc ccagatttt tctacccca 
      ccatgtttt tctattttc tggcgtaca atgtgggcc 
      ccggtcgcc tcaaccaga tattcctgc ggttggcca 
      acctcatgc cgcgcaaaa ccttggttg gggcgtcac 
      atgcggacg acttgagct acattttcc ggccgattt 
      tgggcgaaa aagtgcgct ccgggagcg aggaggaaa 
      tttacatga ggcggttta tccgacttt gggccaatt 
      ttgcaagaa ctgggaacc ccaccccgg gcacaagcg 
      acccccttt tgaatggtg ttgagtgga aaccggtga 
      ctaaagacc aaaactgca tcctggaca tcggcaccc 
      gactcgaga tgaaaaatt acccacacg tcgaaagga 
      tgcagtttt gggacagca tttacagtt actgaagtt 
      tacaataaa tatcaccca caata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY