Status
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Gene information
General information
TC009692 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4619819..4621906
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009692
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04682

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009692 RA
      MANPVVTIK DGQIRGVTD SDYLGKPYH CFLGIPYGK 
      APVGDLRFK APVPVNPWK GIRDATQEG PECPSRHLL 
      FQTYVGSED NCLHVNVYT PQLPDDGNN NLKPVMVWF 
      HGGGFLYDS NKREMYGPD YLITEDVVI VSVSYRLGV 
      LGFLSLEDP TLEIPGNAG LKDMVLALQ WIQNNIQYF 
      CGDPNNVTI FGESAGSVA VHYLYLSPK TKGLFHKAI 
      CQSGCALNT WGEGSTNGL IIAKHLGYN ETNERKILE 
      YLKTLSPQK LIKAQHRFE DTFVASKVQ KFGPVVEKY 
      VTESAFLTE EPLEIIKSG KFNHVPLII GTTSREGML 
      AEAARRLLG KDIIGSSLE DEIHYRLKV KKGSEESRQ 
      IANKIKKFY FGEEEKNPD KYYLLKTDN MFLHATYKT 
      VNYQKKISQ APIYFYEMS LGSPFSLFK SMCTPKLFY 
      PTMIFFLLA YITRDSVFN TFFLRLAKL MPRKNLIGA 
      SHGDDLSYL FKPVLSEKV RPGSDEEIH VKRFIKLWA 
      NFAKTGNPT PDKNDPFLN IEWKPVTKE ENYLLDIGT 
      RLELKTDPN AERMAFWDS IYNFET
    CDS sequence
    • >TC009692 RA
      atggcaaat ccggttgtg acaattaaa gacggacaa 
      attagggga gtcactgat agtgattac ttgggcaag 
      ccctatcac tgtttcttg ggaattccc tacgggaaa 
      gctccagtt ggcgattta cgatttaag gcccctgtt 
      ccagtgaac ccctggaaa gggatacga gatgccaca 
      caagaaggg ccagaatgc ccttcacga cacttactg 
      ttccaaact tacgttggt tctgaggat aattgttta 
      cacgttaat gtttacact ccacagctt ccggatgac 
      ggtaataat aatttgaaa cccgtcatg gtctggttc 
      catggtggt ggctttctg tacgattca aacaaacgc 
      gaaatgtac ggcccagat tatttaata accgaagat 
      gttgttata gtttcggtc agttatcgt ttaggagtc 
      cttggattt ctcagcttg gaagaccca acgcttgaa 
      attcccgga aacgccggg cttaaggac atggttttg 
      gcccttcag tggatacaa aacaacatc caatatttt 
      tgtggagac ccgaacaat gttacaatt tttggcgaa 
      agtgccggt tctgtggca gttcattat ttgtatctt 
      tctcctaaa accaaagga ctgttccac aaagcaatt 
      tgccaaagt gggtgtgcg ctaaatact tggggagag 
      ggaagtacc aatggttta ataattgca aaacatttg 
      ggttacaat gaaacgaac gaacgaaaa attttggag 
      tatttgaaa actttgtca ccacaaaaa ctcataaag 
      gcccaacat cgatttgaa gacacattt gtggcaagt 
      aaagtgcaa aaatttggc ccagttgtt gaaaaatat 
      gtaactgag tccgctttc ttaaccgaa gaaccgctc 
      gaaattatt aaatcgggg aaattcaat cacgttccg 
      ttaataatc gggacaact tcaagggaa ggaatgctt 
      gcagaagct gctagaaga ctactaggc aaagatata 
      atcggttca agcctcgaa gacgaaatc cattaccgg 
      ttgaaagtg aaaaaaggt tcagaagaa tcgagacaa 
      attgccaac aaaatcaag aaattttat ttcggtgaa 
      gaggaaaaa aacccggac aaatattac ctgctcaag 
      accgacaac atgttcctc catgcaact tacaagaca 
      gttaattac cagaaaaaa atcagtcaa gccccgatt 
      tacttctac gaaatgtcc cttgggtcg cctttcagt 
      ctcttcaaa agcatgtgc acccctaaa ttattttac 
      ccaacaatg attttcttc ctattggcg tacataacg 
      cgtgatagt gtatttaac acatttttt ctccgactg 
      gctaaatta atgccgagg aaaaatcta atcggagcg 
      tcacatggc gacgatttg agctacctt ttcaagccc 
      gtcttgagc gagaaagtc aggcctgga agtgacgag 
      gaaattcat gtgaaacgg tttataaaa ctgtgggcg 
      aattttgcc aagaccgga aatccaacg cccgacaaa 
      aacgaccca tttttaaat atcgagtgg aaacccgtg 
      acgaaagaa gaaaattat ttgctcgat atagggact 
      cgattggag ttaaaaaca gatccgaac gccgaaaga 
      atggcgttc tgggacagc atttataat tttgagacg 
      taa
    mRNA sequence
    • >TC009692 RA
      tcattttaa tgttactag cgcaatggc aaatccggt 
      tgtgacaat taaagacgg acaaattag gggagtcac 
      tgatagtga ttacttggg caagcccta tcactgttt 
      cttgggaat tccctacgg gaaagctcc agttggcga 
      tttacgatt taaggcccc tgttccagt gaacccctg 
      gaaagggat acgagatgc cacacaaga agggccaga 
      atgcccttc acgacactt actgttcca aacttacgt 
      tggttctga ggataattg tttacacgt taatgttta 
      cactccaca gcttccgga tgacggtaa taataattt 
      gaaacccgt catggtctg gttccatgg tggtggctt 
      tctgtacga ttcaaacaa acgcgaaat gtacggccc 
      agattattt aataaccga agatgttgt tatagtttc 
      ggtcagtta tcgtttagg agtccttgg atttctcag 
      cttggaaga cccaacgct tgaaattcc cggaaacgc 
      cgggcttaa ggacatggt tttggccct tcagtggat 
      acaaaacaa catccaata tttttgtgg agacccgaa 
      caatgttac aatttttgg cgaaagtgc cggttctgt 
      ggcagttca ttatttgta tctttctcc taaaaccaa 
      aggactgtt ccacaaagc aatttgcca aagtgggtg 
      tgcgctaaa tacttgggg agagggaag taccaatgg 
      tttaataat tgcaaaaca tttgggtta caatgaaac 
      gaacgaacg aaaaatttt ggagtattt gaaaacttt 
      gtcaccaca aaaactcat aaaggccca acatcgatt 
      tgaagacac atttgtggc aagtaaagt gcaaaaatt 
      tggcccagt tgttgaaaa atatgtaac tgagtccgc 
      tttcttaac cgaagaacc gctcgaaat tattaaatc 
      ggggaaatt caatcacgt tccgttaat aatcgggac 
      aacttcaag ggaaggaat gcttgcaga agctgctag 
      aagactact aggcaaaga tataatcgg ttcaagcct 
      cgaagacga aatccatta ccggttgaa agtgaaaaa 
      aggttcaga agaatcgag acaaattgc caacaaaat 
      caagaaatt ttatttcgg tgaagagga aaaaaaccc 
      ggacaaata ttacctgct caagaccga caacatgtt 
      cctccatgc aacttacaa gacagttaa ttaccagaa 
      aaaaatcag tcaagcccc gatttactt ctacgaaat 
      gtcccttgg gtcgccttt cagtctctt caaaagcat 
      gtgcacccc taaattatt ttacccaac aatgatttt 
      cttcctatt ggcgtacat aacgcgtga tagtgtatt 
      taacacatt ttttctccg actggctaa attaatgcc 
      gaggaaaaa tctaatcgg agcgtcaca tggcgacga 
      tttgagcta ccttttcaa gcccgtctt gagcgagaa 
      agtcaggcc tggaagtga cgaggaaat tcatgtgaa 
      acggtttat aaaactgtg ggcgaattt tgccaagac 
      cggaaatcc aacgcccga caaaaacga cccattttt 
      aaatatcga gtggaaacc cgtgacgaa agaagaaaa 
      ttatttgct cgatatagg gactcgatt ggagttaaa 
      aacagatcc gaacgccga aagaatggc gttctggga 
      cagcattta taattttga gacgtaaat aaagtattg 
      tgaacgaaa accgagtct aattttcct ataactata 
      atcccacag aacctggtt tataaagtt aaaatatca 
      aatgttcta attttttgc acagcttag ctttcaata 
      cagtgattg aaagtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY