| General information | |||
| TC034774 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3849277..3891328 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034774 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04679 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04681 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC034774 RA
MRWSLVLAF SVAFSSGFL LDGSTNSYS QFRKWGGGT NGTLEFEFK TDQANGLLL YTDDGGTYD FFEIKLVEG ALRLRYNLG GGAQIITVG RDLNDGHWH KVHVQRHED RTILTVDSV SQMRTSRGK EFNFGRFAT NSDVFVGGM PTWYNTKLT LLALPSVIF EPRFVGAIR NLIYPDVEG GAPRRQETR PKDHRILRG NSSDACEIR DPCQHGGIC ISTDSGPIC ECRNPDYEG EYCEKDKAP SEAVFRGGE FLSYDLSQT GGEPIVSAQ DSVTFYFKT RQPNGLLFY TGDGTDYLN VAIKEGCLS LTMGLSNGK QEMQIKPNK VRFDDNQWH KVSVHRRIQ EISAITSFC RLSAVVDGV YADHSHIAG KFTMLSSSR VYVGGSINT RALPGARVH TNFVGCMRK VEFVADTLR LNLLELGRS GSHLISVAG QLDYKCPAG ESHDPITFT TRDSHLILP PWNAKKSGN ISFKFRTNE ANGLILFNG GVRPPRVDL FAIEIYNGH IYVHLDLGS GHSKQRGSR RRIDDGSWH EVTFRRTAR DARITVDGF HTDFKTIEG STSLELDGN MYVGGLGPP FSEIPIPAG LWTAVLQQG FVGCFKDLV MNNEAVDVA SYAREQDSV MKPCLNGGV CTEGWNRFV CDCTNTMFT GPTCGKEAA TLSFNGTQH MEVTMDSEM TTQTEDIIL RFRTSKPLG LLLITSTVE TGDRIELAV AAGRIRMAL RLGVKEKKQ EDREKDKIL LAGQNVNDN EWHTVRFSR RGANLKLQL DGQSPIRAE TQGKYNSLQ WRTVHLGGL YHLEEEISM TTTVPNFIG EIQQFYFNN IPYIELARA LSSEQTISG FPKIKVAAK FVKHATDNL HRPVTFRSK HTFIGLPML RAYSSIHID FMFKTREAN GLIMFNGGR KEDFVAVEL VDGHINYIV NVGDGTVTL RDTVRSHLN DNRWHTVGI RRPSVKQHT LMVDDDIVI ATNHGTGNL ELDGILYLG GVHKDLYAQ LPQEDVKSR HGFEGCIAG LDLNGESPN IMEDAVVHS SLVTPGCES QSAKCSHNV CANGGICVQ QWHSYTCDC DMTSFTGPT CSDESVSYE FGPNRGIIT YTFPEDNRP EMQEDTIAL GFITTKADA VLLRIVSGT SNDYIEMEI VEGNIFVVY NLGSNDHPL GEISLKVND NAYHVVRFH RQGYNSSLQ IDDYNVQTS HPSGHQLQV FNSQSQIQI GGKWSKGKS RIERPFTGI ISGVVVNRL RVLDLAAEK DIHASLRGD VQLISGILD RHDHLQKMQ QTPASGFPG VEDDLVFSG AGSGCNGDD EDECPPLPE TGSGDDDLI TPVYNPLTK PTPTTKKPH KAAQEGGKP CDDEDCFTG SGSGEVTEE TAITSHTPD VSVTTPDNT SVKTSDAST PTSQTPGSS IPTSSSEKT TEYSSTTPQ VTTLAETTL LVTTEEFPR TSTEKITYI YRPPPDDKI FNTKTKYKP TERITTKTS DTVALIIGI IAAALIAVI LIILVILKF KPRNDGAFK VEDKGYQQG PLLGPSTTN GHQTQYQLN GALRNGDKN QMQKKKRDS KDIKEWYV
- >TC034774 RB
MRWSLVLAF SVAFSSGFL LDGSTNSYS QFRKWGGGT NGTLEFEFK TDQANGLLL YTDDGGTYD FFEIKLVEG ALRLRYNLG GGAQIITVG RDLNDGHWH KVHVQRHED RTILTVDSV SQMRTSRGK EFNFGRFAT NSDVFVGGM PTWYNTKLT LLALPSVIF EPRFVGAIR NLIYPDVEG GAPRRQETR PKDHRCDGG IIAGTEGVC LPQSRILRG NSSDACEIR DPCQHGGIC ISTDSGPIC ECRNPDYEG EYCEKDKAP SEAVFRGGE FLSYDLSQT GGEPIVSAQ DSVTFYFKT RQPNGLLFY TGDGTDYLN VAIKEGCLS LTMGLSNGK QEMQIKPNK VRFDDNQWH KVSVHRRIQ EISAITSFC RLSAVVDGV YADHSHIAG KFTMLSSSR VYVGGSINT RALPGARVH TNFVGCMRK VEFVADTLR LNLLELGRS GSHLISVAG QLDYKCPAG ESHDPITFT TRDSHLILP PWNAKKSGN ISFKFRTNE ANGLILFNG GVRPPRVDL FAIEIYNGH IYVHLDLGS GHSKQRGSR RRIDDGSWH EVTFRRTAR DARITVDGF HTDFKTIEG STSLELDGN MYVGGLGPP FSEIPIPAG LWTAVLQQG FVGCFKDLV MNNEAVDVA SYAREQDSV MKPCLNGGV CTEGWNRFV CDCTNTMFT GPTCGKEAA TLSFNGTQH MEVTMDSEM TTQTEDIIL RFRTSKPLG LLLITSTVE TGDRIELAV AAGRIRMAL RLGVKEKKQ EDREKDKIL LAGQNVNDN EWHTVRFSR RGANLKLQL DGQSPIRAE TQGKYNSLQ WRTVHLGGL YHLEEEISM TTTVPNFIG EIQQFYFNN IPYIELARA LSSEQTISG FPKIKVAAK FVKHATDNL HRPVTFRSK HTFIGLPML RAYSSIHID FMFKTREAN GLIMFNGGR KEDFVAVEL VDGHINYIV NVGDGTVTL RDTVRSHLN DNRWHTVGI RRPSVKQHT LMVDDDIVI ATNHGTGNL ELDGILYLG GVHKDLYAQ LPQEDVKSR HGFEGCIAG LDLNGESPN IMEDAVVHS SLVTPGCES QSAKCSHNV CANGGICVQ QWHSYTCDC DMTSFTGPT CSDESVSYE FGPNRGIIT YTFPEDNRP EMQEDTIAL GFITTKADA VLLRIVSGT SNDYIEMEI VEGNIFVVY NLGSNDHPL GEISLKVND NAYHVVRFH RQGYNSSLQ IDDYNVQTS HPSGHQLQV FNSQSQIQI GGKWSKGKS RIERPFTGI ISGVVVNRL RVLDLAAEK DIHASLRGD VQLISGILD RHDHLQKMQ QTPASGFPG VEDDLVFSG AGSGCNGDD EDECPPLPE TGSGDDDLI TPVYNPLTK PTPTTKKPH KAAQEGGKP CDDEDCFTG SGSGEVTEE TAITSHTPD VSVTTPDNT SVKTSDAST PTSQTPGSS IPTSSSEKT TEYSSTTPQ VTTLAETTL LVTTEEFPR TSTEKITYI YRPPPDDKI FNTKTKYKP TERITTKTS DTVALIIGI IAAALIAVI LIILVILKF KPRNDGAFK VEDKGYQQG PLLGPSTTN GHQTQYQLN GALRNGDKN QMQKKKRDS KDIKEWYV
- >TC034774 RA
atgcggtgg tccttggtg ttagccttc agcgtggcg ttcagctcc ggcttcctg ctcgacggc tccaccaac tcctactcc cagttccgc aagtggggc ggcggcacc aacggcacc ctcgagttc gagttcaag acggaccag gccaacggc ctgctgctc tacaccgac gacggcggc acctacgac ttcttcgag atcaagctg gtggagggc gcgctccgg ctgcgctac aacctgggg ggcggcgcg cagatcatc accgtgggc cgcgacctc aacgacggc cactggcac aaggtgcac gtgcagcgg cacgaggac aggaccatc ctcacggtg gacagcgtc tcgcagatg cgcacctcc aggggcaag gagttcaat ttcgggagg tttgccacc aattcggac gtttttgtc ggggggatg cccacgtgg tacaacacc aagttgacg ctgctggcg ctgccgagt gttattttc gagccgagg tttgtgggg gctatcagg aatctcatc tatcccgat gtggaaggg ggcgctccg aggaggcag gagaccagg ccgaaggat cataggatc ctccgtggc aacagcagc gacgcttgc gaaattcgg gacccttgc caacacggc ggcatctgt atttccact gatagcggt cccatctgc gagtgtcgc aatcccgac tacgagggg gaatattgc gaaaaagat aaagcccca tcggaggcg gttttccga ggtggtgag tttttatcg tacgatctc agtcaaact gggggcgaa cccatcgtc agtgctcaa gactccgtt acgttttat ttcaagacg cgacaaccc aacggactg ttgttttac acaggcgac ggaactgat tatctcaac gtcgcgatt aaagaaggt tgccttagt ctcaccatg ggcctatcg aacggcaaa caagaaatg caaataaag ccgaataaa gtgcgtttc gacgacaat cagtggcac aaggtcagc gtccatcgg cggatacaa gaaatctcg gccatcacc agcttttgt cggctctcg gctgtggtg gacggggtc tacgccgac cactcccac atcgctggt aagttcaca atgttgtcg tcgagtcgt gtctatgtg ggtggaagt atcaacact cgtgcgctt cccggggcc cgagttcac accaacttc gtcggttgt atgcgaaaa gtagaattt gtggcggat acgttacgg ttaaacctt ctggagttg ggtcgttcc ggtagccac ctcatctcc gtggcgggc caacttgat tacaaatgt ccggcaggg gagtcccac gaccccatc accttcacc acacgggat tcgcacttg atattgcca ccttggaac gctaaaaaa tcgggcaac atctcgttc aaattccgc acaaacgag gcgaacggc ttgatcctg ttcaacggc ggagttcgt cctcctcga gtggactta ttcgcgatt gaaatctac aacgggcac atttacgtg cacctggac ctgggttcg ggccactcg aagcagcgc ggctccaga cggaggatc gacgacggc tcctggcac gaagtcacc ttccggcgg accgcccga gacgcgcga atcaccgtc gacggcttc cacaccgat ttcaagacc attgagggt tcaaccagt ctggagctt gatgggaac atgtacgtg ggcggttta ggcccgccg ttctccgag attcccatc ccggctgga ttgtggact gctgtctta caacaggga tttgtggga tgttttaag gacctcgtt atgaacaac gaggcggtt gacgtcgcc agctacgcc cgagagcaa gactccgtt atgaaaccg tgcctcaac ggcggcgtc tgcaccgag ggttggaac cgcttcgtc tgcgactgc accaacacc atgttcacg ggccccacc tgcggcaag gaggccgcc acgttgagc ttcaacggc acccagcac atggaagtg accatggac tcggagatg accacccaa accgaagac ataatcctc cgcttccgc accagcaag cccctcggc ttgctcctc atcaccagc accgtcgag accggcgac cggatcgaa ctcgccgtg gctgcaggt cgcatccga atggctctg cggctcgga gtcaaggag aagaagcag gaggacagg gaaaaggac aaaatactc ctggccggg cagaacgtc aacgataac gagtggcac acggtgagg ttttcgaga cgtggcgca aacctgaag ctgcagctg gatgggcaa agcccgatc cgggccgag acccagggg aagtacaac agtctccag tggcgcacg gtgcacttg ggcgggctg taccacctg gaggaggag attagcatg acgacgacg gtgcccaac tttatcggg gaaatccag cagttttat ttcaataat atcccgtat attgagctg gcgcgggcc ttgagcagc gaacagacc atttccggg tttcccaaa attaaagtg gcggcgaag ttcgtcaag cacgccact gataattta cacagaccg gtcactttc cgctctaaa cacaccttc attgggctg ccaatgctc agagcctac tccagcatc cacatcgat tttatgttc aagacgcgg gaagccaac ggtttgatt atgtttaac ggcgggagg aaagaagat tttgttgca gttgagcta gtcgatggt catatcaat tatatagtc aatgtgggt gatggtaca gtgacttta cgggacact gtcaggtcg catttgaac gacaaccgc tggcatacc gttggtata aggcggccg tcggtcaaa cagcacact ttgatggtg gatgatgat atcgtgata gcgaccaat catgggact gggaatttg gagctggat gggattttg taccttggt ggggttcac aaggatttg tacgcgcag ttgccgcaa gaggacgtt aagtcgcgg catgggttt gagggttgc atcgctggg ttggatctg aatggggag tccccgaat attatggag gatgcagtg gtgcacagc tcgctggta acgccaggc tgtgagagc cagtcggcg aaatgtagc cataatgtg tgtgcgaat ggggggatt tgtgtccag cagtggcac tcgtatacg tgcgattgt gatatgact tcgttcacg ggacctact tgctcagat gagtcggtt tcttatgaa ttcgggcct aacagaggc ataatcact tacacattc cctgaggat aacagaccc gaaatgcaa gaagataca atcgcgttg ggttttatc acaacgaaa gctgatgcc gttttattg aggatcgtc agtgggacg tctaatgat tacatcgaa atggaaatt gtggaagga aacattttt gttgtttat aacttaggc tcaaacgat catccctta ggcgaaatc tcgttgaaa gtcaacgac aatgcatat catgtagtg cgatttcat agacaaggt tacaattcc tcgctccag attgacgat tacaacgtg caaacgagt cacccttca ggccaccag ttgcaagtt ttcaacagc caatcccaa atccagatt ggtggcaaa tggagcaaa ggcaaatca cggatcgag cgccctttc accggaatc atctccgga gtggtcgtt aaccgcctg cgagtccta gacttagcg gccgaaaaa gacatccat gcatcgctg cgtggcgac gtccagttg atttcgggg attttggac cgccacgac cacttgcaa aaaatgcaa caaactccg gcgtccgga tttccgggg gtggaggac gacctggtg ttcagcggc gccggttcc gggtgcaac ggcgacgat gaggatgag tgtccgccg ttaccggaa acggggtcc ggggacgat gatttgata actccggtc tacaacccg ttgactaaa ccgaccccg actacgaaa aagccgcac aaagcggca caagaaggc gggaaaccg tgcgatgat gaggactgt tttaccgga tcggggtcc ggggaggtc accgaggag acggcaatc acgtcacac acaccagat gtgagtgtg acaaccccc gacaacacc agcgtgaaa acctccgac gcttcaacc ccaacatcc caaactccc ggttcgtca atccccacc tcatccagt gaaaaaacc acggaatat tcatcaaca acgccccaa gtcaccact ttggccgag accacgctc ctggtgaca accgaagag ttccctcga acatcaacc gagaaaatc acctacatc taccgccct ccccccgac gacaaaatc ttcaacacg aagaccaaa tacaagccg actgaacgc atcacgacc aaaacctcg gacactgtg gccctcata atcggcata atcgccgcc gcgctgatc gccgtcatc ctcataatc ctggtgatc ttgaagttc aagccgcgg aacgacggc gccttcaag gtggaggac aagggctac cagcagggg ccgctgctg ggccccagc accaccaac ggccaccag acgcagtac caactcaac ggtgctttg cggaatggt gacaagaac cagatgcaa aaaaagaaa cgcgacagt aaagacatt aaagaatgg tatgtgtaa
- >TC034774 RB
atgcggtgg tccttggtg ttagccttc agcgtggcg ttcagctcc ggcttcctg ctcgacggc tccaccaac tcctactcc cagttccgc aagtggggc ggcggcacc aacggcacc ctcgagttc gagttcaag acggaccag gccaacggc ctgctgctc tacaccgac gacggcggc acctacgac ttcttcgag atcaagctg gtggagggc gcgctccgg ctgcgctac aacctgggg ggcggcgcg cagatcatc accgtgggc cgcgacctc aacgacggc cactggcac aaggtgcac gtgcagcgg cacgaggac aggaccatc ctcacggtg gacagcgtc tcgcagatg cgcacctcc aggggcaag gagttcaat ttcgggagg tttgccacc aattcggac gtttttgtc ggggggatg cccacgtgg tacaacacc aagttgacg ctgctggcg ctgccgagt gttattttc gagccgagg tttgtgggg gctatcagg aatctcatc tatcccgat gtggaaggg ggcgctccg aggaggcag gagaccagg ccgaaggat cataggtgt gacggtggc ataattgcg ggaactgaa ggtgtctgt ctgccacag tctaggatc ctccgtggc aacagcagc gacgcttgc gaaattcgg gacccttgc caacacggc ggcatctgt atttccact gatagcggt cccatctgc gagtgtcgc aatcccgac tacgagggg gaatattgc gaaaaagat aaagcccca tcggaggcg gttttccga ggtggtgag tttttatcg tacgatctc agtcaaact gggggcgaa cccatcgtc agtgctcaa gactccgtt acgttttat ttcaagacg cgacaaccc aacggactg ttgttttac acaggcgac ggaactgat tatctcaac gtcgcgatt aaagaaggt tgccttagt ctcaccatg ggcctatcg aacggcaaa caagaaatg caaataaag ccgaataaa gtgcgtttc gacgacaat cagtggcac aaggtcagc gtccatcgg cggatacaa gaaatctcg gccatcacc agcttttgt cggctctcg gctgtggtg gacggggtc tacgccgac cactcccac atcgctggt aagttcaca atgttgtcg tcgagtcgt gtctatgtg ggtggaagt atcaacact cgtgcgctt cccggggcc cgagttcac accaacttc gtcggttgt atgcgaaaa gtagaattt gtggcggat acgttacgg ttaaacctt ctggagttg ggtcgttcc ggtagccac ctcatctcc gtggcgggc caacttgat tacaaatgt ccggcaggg gagtcccac gaccccatc accttcacc acacgggat tcgcacttg atattgcca ccttggaac gctaaaaaa tcgggcaac atctcgttc aaattccgc acaaacgag gcgaacggc ttgatcctg ttcaacggc ggagttcgt cctcctcga gtggactta ttcgcgatt gaaatctac aacgggcac atttacgtg cacctggac ctgggttcg ggccactcg aagcagcgc ggctccaga cggaggatc gacgacggc tcctggcac gaagtcacc ttccggcgg accgcccga gacgcgcga atcaccgtc gacggcttc cacaccgat ttcaagacc attgagggt tcaaccagt ctggagctt gatgggaac atgtacgtg ggcggttta ggcccgccg ttctccgag attcccatc ccggctgga ttgtggact gctgtctta caacaggga tttgtggga tgttttaag gacctcgtt atgaacaac gaggcggtt gacgtcgcc agctacgcc cgagagcaa gactccgtt atgaaaccg tgcctcaac ggcggcgtc tgcaccgag ggttggaac cgcttcgtc tgcgactgc accaacacc atgttcacg ggccccacc tgcggcaag gaggccgcc acgttgagc ttcaacggc acccagcac atggaagtg accatggac tcggagatg accacccaa accgaagac ataatcctc cgcttccgc accagcaag cccctcggc ttgctcctc atcaccagc accgtcgag accggcgac cggatcgaa ctcgccgtg gctgcaggt cgcatccga atggctctg cggctcgga gtcaaggag aagaagcag gaggacagg gaaaaggac aaaatactc ctggccggg cagaacgtc aacgataac gagtggcac acggtgagg ttttcgaga cgtggcgca aacctgaag ctgcagctg gatgggcaa agcccgatc cgggccgag acccagggg aagtacaac agtctccag tggcgcacg gtgcacttg ggcgggctg taccacctg gaggaggag attagcatg acgacgacg gtgcccaac tttatcggg gaaatccag cagttttat ttcaataat atcccgtat attgagctg gcgcgggcc ttgagcagc gaacagacc atttccggg tttcccaaa attaaagtg gcggcgaag ttcgtcaag cacgccact gataattta cacagaccg gtcactttc cgctctaaa cacaccttc attgggctg ccaatgctc agagcctac tccagcatc cacatcgat tttatgttc aagacgcgg gaagccaac ggtttgatt atgtttaac ggcgggagg aaagaagat tttgttgca gttgagcta gtcgatggt catatcaat tatatagtc aatgtgggt gatggtaca gtgacttta cgggacact gtcaggtcg catttgaac gacaaccgc tggcatacc gttggtata aggcggccg tcggtcaaa cagcacact ttgatggtg gatgatgat atcgtgata gcgaccaat catgggact gggaatttg gagctggat gggattttg taccttggt ggggttcac aaggatttg tacgcgcag ttgccgcaa gaggacgtt aagtcgcgg catgggttt gagggttgc atcgctggg ttggatctg aatggggag tccccgaat attatggag gatgcagtg gtgcacagc tcgctggta acgccaggc tgtgagagc cagtcggcg aaatgtagc cataatgtg tgtgcgaat ggggggatt tgtgtccag cagtggcac tcgtatacg tgcgattgt gatatgact tcgttcacg ggacctact tgctcagat gagtcggtt tcttatgaa ttcgggcct aacagaggc ataatcact tacacattc cctgaggat aacagaccc gaaatgcaa gaagataca atcgcgttg ggttttatc acaacgaaa gctgatgcc gttttattg aggatcgtc agtgggacg tctaatgat tacatcgaa atggaaatt gtggaagga aacattttt gttgtttat aacttaggc tcaaacgat catccctta ggcgaaatc tcgttgaaa gtcaacgac aatgcatat catgtagtg cgatttcat agacaaggt tacaattcc tcgctccag attgacgat tacaacgtg caaacgagt cacccttca ggccaccag ttgcaagtt ttcaacagc caatcccaa atccagatt ggtggcaaa tggagcaaa ggcaaatca cggatcgag cgccctttc accggaatc atctccgga gtggtcgtt aaccgcctg cgagtccta gacttagcg gccgaaaaa gacatccat gcatcgctg cgtggcgac gtccagttg atttcgggg attttggac cgccacgac cacttgcaa aaaatgcaa caaactccg gcgtccgga tttccgggg gtggaggac gacctggtg ttcagcggc gccggttcc gggtgcaac ggcgacgat gaggatgag tgtccgccg ttaccggaa acggggtcc ggggacgat gatttgata actccggtc tacaacccg ttgactaaa ccgaccccg actacgaaa aagccgcac aaagcggca caagaaggc gggaaaccg tgcgatgat gaggactgt tttaccgga tcggggtcc ggggaggtc accgaggag acggcaatc acgtcacac acaccagat gtgagtgtg acaaccccc gacaacacc agcgtgaaa acctccgac gcttcaacc ccaacatcc caaactccc ggttcgtca atccccacc tcatccagt gaaaaaacc acggaatat tcatcaaca acgccccaa gtcaccact ttggccgag accacgctc ctggtgaca accgaagag ttccctcga acatcaacc gagaaaatc acctacatc taccgccct ccccccgac gacaaaatc ttcaacacg aagaccaaa tacaagccg actgaacgc atcacgacc aaaacctcg gacactgtg gccctcata atcggcata atcgccgcc gcgctgatc gccgtcatc ctcataatc ctggtgatc ttgaagttc aagccgcgg aacgacggc gccttcaag gtggaggac aagggctac cagcagggg ccgctgctg ggccccagc accaccaac ggccaccag acgcagtac caactcaac ggtgctttg cggaatggt gacaagaac cagatgcaa aaaaagaaa cgcgacagt aaagacatt aaagaatgg tatgtgtaa
- >TC034774 RA
ccggttgta aatcaccag tgtcagtgt tagaataaa gccatttca gacaagtgc attggttag gcggtgaga atcagtcgc gccatgaac agttccata ttctcctcc cctctcgaa gccgcgtcc ccaaccccc ttcgaatcg atatctcaa gcgtgttaa tgctccaag catcatcat catcatgcg gtggtcctt ggtgttagc cttcagcgt ggcgttcag ctccggctt cctgctcga cggctccac caactccta ctcccagtt ccgcaagtg gggcggcgg caccaacgg caccctcga gttcgagtt caagacgga ccaggccaa cggcctgct gctctacac cgacgacgg cggcaccta cgacttctt cgagatcaa gctggtgga gggcgcgct ccggctgcg ctacaacct ggggggcgg cgcgcagat catcaccgt gggccgcga cctcaacga cggccactg gcacaaggt gcacgtgca gcggcacga ggacaggac catcctcac ggtggacag cgtctcgca gatgcgcac ctccagggg caaggagtt caatttcgg gaggtttgc caccaattc ggacgtttt tgtcggggg gatgcccac gtggtacaa caccaagtt gacgctgct ggcgctgcc gagtgttat tttcgagcc gaggtttgt gggggctat caggaatct catctatcc cgatgtgga agggggcgc tccgaggag gcaggagac caggccgaa ggatcatag gatcctccg tggcaacag cagcgacgc ttgcgaaat tcgggaccc ttgccaaca cggcggcat ctgtatttc cactgatag cggtcccat ctgcgagtg tcgcaatcc cgactacga gggggaata ttgcgaaaa agataaagc cccatcgga ggcggtttt ccgaggtgg tgagttttt atcgtacga tctcagtca aactggggg cgaacccat cgtcagtgc tcaagactc cgttacgtt ttatttcaa gacgcgaca acccaacgg actgttgtt ttacacagg cgacggaac tgattatct caacgtcgc gattaaaga aggttgcct tagtctcac catgggcct atcgaacgg caaacaaga aatgcaaat aaagccgaa taaagtgcg tttcgacga caatcagtg gcacaaggt cagcgtcca tcggcggat acaagaaat ctcggccat caccagctt ttgtcggct ctcggctgt ggtggacgg ggtctacgc cgaccactc ccacatcgc tggtaagtt cacaatgtt gtcgtcgag tcgtgtcta tgtgggtgg aagtatcaa cactcgtgc gcttcccgg ggcccgagt tcacaccaa cttcgtcgg ttgtatgcg aaaagtaga atttgtggc ggatacgtt acggttaaa ccttctgga gttgggtcg ttccggtag ccacctcat ctccgtggc gggccaact tgattacaa atgtccggc aggggagtc ccacgaccc catcacctt caccacacg ggattcgca cttgatatt gccaccttg gaacgctaa aaaatcggg caacatctc gttcaaatt ccgcacaaa cgaggcgaa cggcttgat cctgttcaa cggcggagt tcgtcctcc tcgagtgga cttattcgc gattgaaat ctacaacgg gcacattta cgtgcacct ggacctggg ttcgggcca ctcgaagca gcgcggctc cagacggag gatcgacga cggctcctg gcacgaagt caccttccg gcggaccgc ccgagacgc gcgaatcac cgtcgacgg cttccacac cgatttcaa gaccattga gggttcaac cagtctgga gcttgatgg gaacatgta cgtgggcgg tttaggccc gccgttctc cgagattcc catcccggc tggattgtg gactgctgt cttacaaca gggatttgt gggatgttt taaggacct cgttatgaa caacgaggc ggttgacgt cgccagcta cgcccgaga gcaagactc cgttatgaa accgtgcct caacggcgg cgtctgcac cgagggttg gaaccgctt cgtctgcga ctgcaccaa caccatgtt cacgggccc cacctgcgg caaggaggc cgccacgtt gagcttcaa cggcaccca gcacatgga agtgaccat ggactcgga gatgaccac ccaaaccga agacataat cctccgctt ccgcaccag caagcccct cggcttgct cctcatcac cagcaccgt cgagaccgg cgaccggat cgaactcgc cgtggctgc aggtcgcat ccgaatggc tctgcggct cggagtcaa ggagaagaa gcaggagga cagggaaaa ggacaaaat actcctggc cgggcagaa cgtcaacga taacgagtg gcacacggt gaggttttc gagacgtgg cgcaaacct gaagctgca gctggatgg gcaaagccc gatccgggc cgagaccca ggggaagta caacagtct ccagtggcg cacggtgca cttgggcgg gctgtacca cctggagga ggagattag catgacgac gacggtgcc caactttat cggggaaat ccagcagtt ttatttcaa taatatccc gtatattga gctggcgcg ggccttgag cagcgaaca gaccatttc cgggtttcc caaaattaa agtggcggc gaagttcgt caagcacgc cactgataa tttacacag accggtcac tttccgctc taaacacac cttcattgg gctgccaat gctcagagc ctactccag catccacat cgattttat gttcaagac gcgggaagc caacggttt gattatgtt taacggcgg gaggaaaga agattttgt tgcagttga gctagtcga tggtcatat caattatat agtcaatgt gggtgatgg tacagtgac tttacggga cactgtcag gtcgcattt gaacgacaa ccgctggca taccgttgg tataaggcg gccgtcggt caaacagca cactttgat ggtggatga tgatatcgt gatagcgac caatcatgg gactgggaa tttggagct ggatgggat tttgtacct tggtggggt tcacaagga tttgtacgc gcagttgcc gcaagagga cgttaagtc gcggcatgg gtttgaggg ttgcatcgc tgggttgga tctgaatgg ggagtcccc gaatattat ggaggatgc agtggtgca cagctcgct ggtaacgcc aggctgtga gagccagtc ggcgaaatg tagccataa tgtgtgtgc gaatggggg gatttgtgt ccagcagtg gcactcgta tacgtgcga ttgtgatat gacttcgtt cacgggacc tacttgctc agatgagtc ggtttctta tgaattcgg gcctaacag aggcataat cacttacac attccctga ggataacag acccgaaat gcaagaaga tacaatcgc gttgggttt tatcacaac gaaagctga tgccgtttt attgaggat cgtcagtgg gacgtctaa tgattacat cgaaatgga aattgtgga aggaaacat ttttgttgt ttataactt aggctcaaa cgatcatcc cttaggcga aatctcgtt gaaagtcaa cgacaatgc atatcatgt agtgcgatt tcatagaca aggttacaa ttcctcgct ccagattga cgattacaa cgtgcaaac gagtcaccc ttcaggcca ccagttgca agttttcaa cagccaatc ccaaatcca gattggtgg caaatggag caaaggcaa atcacggat cgagcgccc tttcaccgg aatcatctc cggagtggt cgttaaccg cctgcgagt cctagactt agcggccga aaaagacat ccatgcatc gctgcgtgg cgacgtcca gttgatttc ggggatttt ggaccgcca cgaccactt gcaaaaaat gcaacaaac tccggcgtc cggatttcc gggggtgga ggacgacct ggtgttcag cggcgccgg ttccgggtg caacggcga cgatgagga tgagtgtcc gccgttacc ggaaacggg gtccgggga cgatgattt gataactcc ggtctacaa cccgttgac taaaccgac cccgactac gaaaaagcc gcacaaagc ggcacaaga aggcgggaa accgtgcga tgatgagga ctgttttac cggatcggg gtccgggga ggtcaccga ggagacggc aatcacgtc acacacacc agatgtgag tgtgacaac ccccgacaa caccagcgt gaaaacctc cgacgcttc aaccccaac atcccaaac tcccggttc gtcaatccc cacctcatc cagtgaaaa aaccacgga atattcatc aacaacgcc ccaagtcac cactttggc cgagaccac gctcctggt gacaaccga agagttccc tcgaacatc aaccgagaa aatcaccta catctaccg ccctccccc cgacgacaa aatcttcaa cacgaagac caaatacaa gccgactga acgcatcac gaccaaaac ctcggacac tgtggccct cataatcgg cataatcgc cgccgcgct gatcgccgt catcctcat aatcctggt gatcttgaa gttcaagcc gcggaacga cggcgcctt caaggtgga ggacaaggg ctaccagca ggggccgct gctgggccc cagcaccac caacggcca ccagacgca gtaccaact caacggtgc tttgcggaa tggtgacaa gaaccagat gcaaaaaaa gaaacgcga cagtaaaga cattaaaga atggtatgt gtaagtgtt tttttttcg ttcgttcga ttgtgactc cgagccatt tgtgatgat gagtgactg aaacaacta acaaagtga tatcttcat actctcttc ggttgtctt atacttacc agtgttgaa gctctacaa tgatggtca gtgttatca aatatgctc attgttgca taagatttt cctaaggct ctatgcaat gcacggaca aagcaggca cggattttt ttagttgtt gacaataag aaacaaaaa taataacga gacccgaat cagttaacg caatacatt tttagtagg ggagtgcta gtacagtca attagtcaa ttaattaaa gggggcgtt taattaaaa aaacatgac aagg
- >TC034774 RB
ccggttgta aatcaccag tgtcagtgt tagaataaa gccatttca gacaagtgc attggttag gcggtgaga atcagtcgc gccatgaac agttccata ttctcctcc cctctcgaa gccgcgtcc ccaaccccc ttcgaatcg atatctcaa gcgtgttaa tgctccaag catcatcat catcatgcg gtggtcctt ggtgttagc cttcagcgt ggcgttcag ctccggctt cctgctcga cggctccac caactccta ctcccagtt ccgcaagtg gggcggcgg caccaacgg caccctcga gttcgagtt caagacgga ccaggccaa cggcctgct gctctacac cgacgacgg cggcaccta cgacttctt cgagatcaa gctggtgga gggcgcgct ccggctgcg ctacaacct ggggggcgg cgcgcagat catcaccgt gggccgcga cctcaacga cggccactg gcacaaggt gcacgtgca gcggcacga ggacaggac catcctcac ggtggacag cgtctcgca gatgcgcac ctccagggg caaggagtt caatttcgg gaggtttgc caccaattc ggacgtttt tgtcggggg gatgcccac gtggtacaa caccaagtt gacgctgct ggcgctgcc gagtgttat tttcgagcc gaggtttgt gggggctat caggaatct catctatcc cgatgtgga agggggcgc tccgaggag gcaggagac caggccgaa ggatcatag gtgtgacgg tggcataat tgcgggaac tgaaggtgt ctgtctgcc acagtctag gatcctccg tggcaacag cagcgacgc ttgcgaaat tcgggaccc ttgccaaca cggcggcat ctgtatttc cactgatag cggtcccat ctgcgagtg tcgcaatcc cgactacga gggggaata ttgcgaaaa agataaagc cccatcgga ggcggtttt ccgaggtgg tgagttttt atcgtacga tctcagtca aactggggg cgaacccat cgtcagtgc tcaagactc cgttacgtt ttatttcaa gacgcgaca acccaacgg actgttgtt ttacacagg cgacggaac tgattatct caacgtcgc gattaaaga aggttgcct tagtctcac catgggcct atcgaacgg caaacaaga aatgcaaat aaagccgaa taaagtgcg tttcgacga caatcagtg gcacaaggt cagcgtcca tcggcggat acaagaaat ctcggccat caccagctt ttgtcggct ctcggctgt ggtggacgg ggtctacgc cgaccactc ccacatcgc tggtaagtt cacaatgtt gtcgtcgag tcgtgtcta tgtgggtgg aagtatcaa cactcgtgc gcttcccgg ggcccgagt tcacaccaa cttcgtcgg ttgtatgcg aaaagtaga atttgtggc ggatacgtt acggttaaa ccttctgga gttgggtcg ttccggtag ccacctcat ctccgtggc gggccaact tgattacaa atgtccggc aggggagtc ccacgaccc catcacctt caccacacg ggattcgca cttgatatt gccaccttg gaacgctaa aaaatcggg caacatctc gttcaaatt ccgcacaaa cgaggcgaa cggcttgat cctgttcaa cggcggagt tcgtcctcc tcgagtgga cttattcgc gattgaaat ctacaacgg gcacattta cgtgcacct ggacctggg ttcgggcca ctcgaagca gcgcggctc cagacggag gatcgacga cggctcctg gcacgaagt caccttccg gcggaccgc ccgagacgc gcgaatcac cgtcgacgg cttccacac cgatttcaa gaccattga gggttcaac cagtctgga gcttgatgg gaacatgta cgtgggcgg tttaggccc gccgttctc cgagattcc catcccggc tggattgtg gactgctgt cttacaaca gggatttgt gggatgttt taaggacct cgttatgaa caacgaggc ggttgacgt cgccagcta cgcccgaga gcaagactc cgttatgaa accgtgcct caacggcgg cgtctgcac cgagggttg gaaccgctt cgtctgcga ctgcaccaa caccatgtt cacgggccc cacctgcgg caaggaggc cgccacgtt gagcttcaa cggcaccca gcacatgga agtgaccat ggactcgga gatgaccac ccaaaccga agacataat cctccgctt ccgcaccag caagcccct cggcttgct cctcatcac cagcaccgt cgagaccgg cgaccggat cgaactcgc cgtggctgc aggtcgcat ccgaatggc tctgcggct cggagtcaa ggagaagaa gcaggagga cagggaaaa ggacaaaat actcctggc cgggcagaa cgtcaacga taacgagtg gcacacggt gaggttttc gagacgtgg cgcaaacct gaagctgca gctggatgg gcaaagccc gatccgggc cgagaccca ggggaagta caacagtct ccagtggcg cacggtgca cttgggcgg gctgtacca cctggagga ggagattag catgacgac gacggtgcc caactttat cggggaaat ccagcagtt ttatttcaa taatatccc gtatattga gctggcgcg ggccttgag cagcgaaca gaccatttc cgggtttcc caaaattaa agtggcggc gaagttcgt caagcacgc cactgataa tttacacag accggtcac tttccgctc taaacacac cttcattgg gctgccaat gctcagagc ctactccag catccacat cgattttat gttcaagac gcgggaagc caacggttt gattatgtt taacggcgg gaggaaaga agattttgt tgcagttga gctagtcga tggtcatat caattatat agtcaatgt gggtgatgg tacagtgac tttacggga cactgtcag gtcgcattt gaacgacaa ccgctggca taccgttgg tataaggcg gccgtcggt caaacagca cactttgat ggtggatga tgatatcgt gatagcgac caatcatgg gactgggaa tttggagct ggatgggat tttgtacct tggtggggt tcacaagga tttgtacgc gcagttgcc gcaagagga cgttaagtc gcggcatgg gtttgaggg ttgcatcgc tgggttgga tctgaatgg ggagtcccc gaatattat ggaggatgc agtggtgca cagctcgct ggtaacgcc aggctgtga gagccagtc ggcgaaatg tagccataa tgtgtgtgc gaatggggg gatttgtgt ccagcagtg gcactcgta tacgtgcga ttgtgatat gacttcgtt cacgggacc tacttgctc agatgagtc ggtttctta tgaattcgg gcctaacag aggcataat cacttacac attccctga ggataacag acccgaaat gcaagaaga tacaatcgc gttgggttt tatcacaac gaaagctga tgccgtttt attgaggat cgtcagtgg gacgtctaa tgattacat cgaaatgga aattgtgga aggaaacat ttttgttgt ttataactt aggctcaaa cgatcatcc cttaggcga aatctcgtt gaaagtcaa cgacaatgc atatcatgt agtgcgatt tcatagaca aggttacaa ttcctcgct ccagattga cgattacaa cgtgcaaac gagtcaccc ttcaggcca ccagttgca agttttcaa cagccaatc ccaaatcca gattggtgg caaatggag caaaggcaa atcacggat cgagcgccc tttcaccgg aatcatctc cggagtggt cgttaaccg cctgcgagt cctagactt agcggccga aaaagacat ccatgcatc gctgcgtgg cgacgtcca gttgatttc ggggatttt ggaccgcca cgaccactt gcaaaaaat gcaacaaac tccggcgtc cggatttcc gggggtgga ggacgacct ggtgttcag cggcgccgg ttccgggtg caacggcga cgatgagga tgagtgtcc gccgttacc ggaaacggg gtccgggga cgatgattt gataactcc ggtctacaa cccgttgac taaaccgac cccgactac gaaaaagcc gcacaaagc ggcacaaga aggcgggaa accgtgcga tgatgagga ctgttttac cggatcggg gtccgggga ggtcaccga ggagacggc aatcacgtc acacacacc agatgtgag tgtgacaac ccccgacaa caccagcgt gaaaacctc cgacgcttc aaccccaac atcccaaac tcccggttc gtcaatccc cacctcatc cagtgaaaa aaccacgga atattcatc aacaacgcc ccaagtcac cactttggc cgagaccac gctcctggt gacaaccga agagttccc tcgaacatc aaccgagaa aatcaccta catctaccg ccctccccc cgacgacaa aatcttcaa cacgaagac caaatacaa gccgactga acgcatcac gaccaaaac ctcggacac tgtggccct cataatcgg cataatcgc cgccgcgct gatcgccgt catcctcat aatcctggt gatcttgaa gttcaagcc gcggaacga cggcgcctt caaggtgga ggacaaggg ctaccagca ggggccgct gctgggccc cagcaccac caacggcca ccagacgca gtaccaact caacggtgc tttgcggaa tggtgacaa gaaccagat gcaaaaaaa gaaacgcga cagtaaaga cattaaaga atggtatgt gtaagtgtt tttttttcg ttcgttcga ttgtgactc cgagccatt tgtgatgat gagtgactg aaacaacta acaaagtga tatcttcat actctcttc ggttgtctt atacttacc agtgttgaa gctctacaa tgatggtca gtgttatca aatatgctc attgttgca taagatttt cctaaggct ctatgcaat gcacggaca aagcaggca cggattttt ttagttgtt gacaataag aaacaaaaa taataacga gacccgaat cagttaacg caatacatt tttagtagg ggagtgcta gtacagtca attagtcaa ttaattaaa gggggcgtt taattaaaa aaacatgac aagg
