Status
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Gene information
General information
TC033602 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3820717..3833519
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033602
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04678

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033602 RA
      MVKRSDGTE SPILEEQES ANSEKPKQL VKYGELVIL 
      GYNGFLPQG DRGRRRSKF VLYKRPTAN GVKRSKHYV 
      VKTPHSSQA ILDANQHSI SYTLSRNHA VIVEYTPDD 
      DTDMFQIGR SSEAPIDFV VMDTVPGDK TGETKVMQS 
      TISRFACRI LCDRKNPKI ARIYAAGFD TSKNIFLGE 
      KATKWQENG REIDGLTTN GVLIMHPRG TFCGGDAKC 
      SYWLETSVG GGVFSLRES RSAQQKGQV VENETNILE 
      DGTLIDLCG ATLLWRSAE GLTKSPSKR DLEREIDEI 
      NAGRPQCPV GLNTLVIPR KVTPNENLQ QPYVYLNCG 
      HVQGLHDWG QDKDSGARK CPICLELGP VVKVFMGLE 
      PAFYVDSGP PTFAFNPCG HMATEKTVK YWANVAIPH 
      GTNGFHAVC PFCASPLAG SPGYVRLIF QDNVD
    • >TC033602 RB
      MDERKMEPK EVSTEVLVK VYCEIEWCF SDSGDNNML 
      IKKMKLSDG TESPILEEQ ESANSEKPK QLVKYGELV 
      ILGYNGFLP QGDRGRRRS KFVLYKRPT ANGVKRSKH 
      YVVKTPHSS QAILDANQH SISYTLSRN HAVIVEYTP 
      DDDTDMFQI GRSSEAPID FVVMDTVPG DKTGETKVM 
      QSTISRFAC RILCDRKNP KIARIYAAG FDTSKNIFL 
      GEKATKWQE NGREIDGLT TNGVLIMHP RGTFCGGDA 
      KCSYWLETS VGGGVFSLR ESRSAQQKG QVVENETNI 
      LEDGTLIDL CGATLLWRS AEGLTKSPS KRDLEREID 
      EINAGRPQC PVGLNTLVI PRKVTPNEN LQQPYVYLN 
      CGHVQGLHD WGQDKDSGA RKCPICLEL GPVVKVFMG 
      LEPAFYVDS GPPTFAFNP CGHMATEKT VKYWANVAI 
      PHGTNGFHA VCPFCASPL AGSPGYVRL IFQDNVD
    CDS sequence
    • >TC033602 RA
      atggtgaaa agatcggat ggcaccgag tctccaata 
      ttagaagaa caagagagt gccaactca gagaaacca 
      aaacaactc gtcaaatat ggagaacta gtcatttta 
      ggatacaac ggttttcta cctcagggc gaccgaggc 
      agacgacgt agcaaattc gtattgtac aaacgacca 
      acagcgaac ggagtcaag agatcgaaa cactatgtc 
      gtcaaaacg ccccattcg tctcaggcc atcctggac 
      gcaaaccag cactcgata tcctacaca ctttcacgc 
      aaccatgcc gtcatagtg gaatacacc cccgacgat 
      gacaccgac atgtttcaa atcggtcgt tcgtcggaa 
      gcccccatc gacttcgtg gtcatggac accgtcccc 
      ggggacaag actggcgaa accaaggtg atgcaaagc 
      acgatatcg cgatttgcg tgtcggatt ttatgcgat 
      aggaagaat ccgaaaatt gcgaggata tacgccgcc 
      ggttttgac acctccaaa aacatattt ctcggggag 
      aaagcaacg aaatggcag gaaaacggg cgcgaaatc 
      gacggtttg accacgaat ggtgtgtta attatgcat 
      ccaagaggg acgttttgt gggggagat gccaagtgt 
      agttattgg ttggagacg tctgttggt ggaggtgtg 
      ttttcgttg agggagtca aggtcggcc caacagaag 
      ggccaagtg gtggaaaac gagacgaat attttggaa 
      gatgggact ttgatcgat ttgtgcggg gctacgctg 
      ttgtggagg tcggcagag gggctcaca aaatcgccg 
      agcaaacgc gacctggag cgcgaaatc gacgaaatc 
      aacgccggt cgcccgcag tgccccgtt ggcttgaac 
      actttagtc attccccga aaagtcaca ccgaacgaa 
      aacctgcaa cagccttac gtttatctc aactgcgga 
      cacgtccaa ggcttgcac gattggggc caagacaag 
      gattcgggc gcccgaaag tgccccatt tgcctagaa 
      ttgggaccg gttgtgaag gtctttatg gggctcgag 
      ccggcgttt tatgtcgat tcggggccc ccgacgttc 
      gcgttcaac ccgtgcggg cacatggcc accgaaaag 
      accgtcaag tactgggca aatgtggct attccgcat 
      ggtacgaat gggtttcac gccgtgtgc cccttttgc 
      gcttcgccc ctggcgggg tctccgggg tacgtccgc 
      ctaattttc caggataat gtggattga
    • >TC033602 RB
      atggacgag agaaaaatg gaaccgaag gaagtttcc 
      actgaagtt ttagttaaa gtgtattgc gaaattgag 
      tggtgtttt agtgactct ggggataat aacatgcta 
      attaagaaa atgaagtta tcggatggc accgagtct 
      ccaatatta gaagaacaa gagagtgcc aactcagag 
      aaaccaaaa caactcgtc aaatatgga gaactagtc 
      attttagga tacaacggt tttctacct cagggcgac 
      cgaggcaga cgacgtagc aaattcgta ttgtacaaa 
      cgaccaaca gcgaacgga gtcaagaga tcgaaacac 
      tatgtcgtc aaaacgccc cattcgtct caggccatc 
      ctggacgca aaccagcac tcgatatcc tacacactt 
      tcacgcaac catgccgtc atagtggaa tacaccccc 
      gacgatgac accgacatg tttcaaatc ggtcgttcg 
      tcggaagcc cccatcgac ttcgtggtc atggacacc 
      gtccccggg gacaagact ggcgaaacc aaggtgatg 
      caaagcacg atatcgcga tttgcgtgt cggatttta 
      tgcgatagg aagaatccg aaaattgcg aggatatac 
      gccgccggt tttgacacc tccaaaaac atatttctc 
      ggggagaaa gcaacgaaa tggcaggaa aacgggcgc 
      gaaatcgac ggtttgacc acgaatggt gtgttaatt 
      atgcatcca agagggacg ttttgtggg ggagatgcc 
      aagtgtagt tattggttg gagacgtct gttggtgga 
      ggtgtgttt tcgttgagg gagtcaagg tcggcccaa 
      cagaagggc caagtggtg gaaaacgag acgaatatt 
      ttggaagat gggactttg atcgatttg tgcggggct 
      acgctgttg tggaggtcg gcagagggg ctcacaaaa 
      tcgccgagc aaacgcgac ctggagcgc gaaatcgac 
      gaaatcaac gccggtcgc ccgcagtgc cccgttggc 
      ttgaacact ttagtcatt ccccgaaaa gtcacaccg 
      aacgaaaac ctgcaacag ccttacgtt tatctcaac 
      tgcggacac gtccaaggc ttgcacgat tggggccaa 
      gacaaggat tcgggcgcc cgaaagtgc cccatttgc 
      ctagaattg ggaccggtt gtgaaggtc tttatgggg 
      ctcgagccg gcgttttat gtcgattcg gggcccccg 
      acgttcgcg ttcaacccg tgcgggcac atggccacc 
      gaaaagacc gtcaagtac tgggcaaat gtggctatt 
      ccgcatggt acgaatggg tttcacgcc gtgtgcccc 
      ttttgcgct tcgcccctg gcggggtct ccggggtac 
      gtccgccta attttccag gataatgtg gattga
    mRNA sequence
    • >TC033602 RA
      ccattgtta tcggtaaaa gtgcggaga gtgtccgtg 
      gaaaagcat caccgtgcg tgtttttcc ccttggaag 
      tcgtgcttt catcgcaaa cggtgcgaa acctccgtg 
      aaagcgtcc gccagtgca acgaagacg gccgtgcct 
      agtgaatgc gtgtgccac cagtgataa acatgcacg 
      aatcggcgg ctctagtga tgtttttag tgaagcaac 
      gacaccagt gattgatat ttttatgcg tttcgaata 
      cgacaatcg cagccaaac tgtttaaga cggcttcag 
      aaatggtga aaagatcgg atggcaccg agtctccaa 
      tattagaag aacaagaga gtgccaact cagagaaac 
      caaaacaac tcgtcaaat atggagaac tagtcattt 
      taggataca acggttttc tacctcagg gcgaccgag 
      gcagacgac gtagcaaat tcgtattgt acaaacgac 
      caacagcga acggagtca agagatcga aacactatg 
      tcgtcaaaa cgccccatt cgtctcagg ccatcctgg 
      acgcaaacc agcactcga tatcctaca cactttcac 
      gcaaccatg ccgtcatag tggaataca cccccgacg 
      atgacaccg acatgtttc aaatcggtc gttcgtcgg 
      aagccccca tcgacttcg tggtcatgg acaccgtcc 
      ccggggaca agactggcg aaaccaagg tgatgcaaa 
      gcacgatat cgcgatttg cgtgtcgga ttttatgcg 
      ataggaaga atccgaaaa ttgcgagga tatacgccg 
      ccggttttg acacctcca aaaacatat ttctcgggg 
      agaaagcaa cgaaatggc aggaaaacg ggcgcgaaa 
      tcgacggtt tgaccacga atggtgtgt taattatgc 
      atccaagag ggacgtttt gtgggggag atgccaagt 
      gtagttatt ggttggaga cgtctgttg gtggaggtg 
      tgttttcgt tgagggagt caaggtcgg cccaacaga 
      agggccaag tggtggaaa acgagacga atattttgg 
      aagatggga ctttgatcg atttgtgcg gggctacgc 
      tgttgtgga ggtcggcag aggggctca caaaatcgc 
      cgagcaaac gcgacctgg agcgcgaaa tcgacgaaa 
      tcaacgccg gtcgcccgc agtgccccg ttggcttga 
      acactttag tcattcccc gaaaagtca caccgaacg 
      aaaacctgc aacagcctt acgtttatc tcaactgcg 
      gacacgtcc aaggcttgc acgattggg gccaagaca 
      aggattcgg gcgcccgaa agtgcccca tttgcctag 
      aattgggac cggttgtga aggtcttta tggggctcg 
      agccggcgt tttatgtcg attcggggc ccccgacgt 
      tcgcgttca acccgtgcg ggcacatgg ccaccgaaa 
      agaccgtca agtactggg caaatgtgg ctattccgc 
      atggtacga atgggtttc acgccgtgt gcccctttt 
      gcgcttcgc ccctggcgg ggtctccgg ggtacgtcc 
      gcctaattt tccaggata atgtggatt gaaaggaat 
      gtagagtag tccaccaaa gctttattt atgtttttg 
      ctcttttat ttaatgtat aattggttt ttagcagtc 
      tcatttatt aattttttg gaaattacc caattgtaa 
      ttacttgta attattgta tataattgc acttgaaga 
      tgggagcga gccgagaat tgcagttgt atcatatca 
      tgatttttt taatggaaa ttgcggttt attacagtg 
      caaatgcgt gaatcgtta agactggtt aggatttag 
      cttataatg taataagta tttaatata aatcgacct 
      gttaatgca atgttttat tcaaaaaaa atattttta 
      catacttaa ttgacccag attttttga ttttatcgt 
      gataacagg ctgtctcaa aattggcga attttagtt 
      cagagcttt gtagagaat tgcttcaat agtccaaat 
      atagaaaaa aaaattaag attctgggt ttcaaccaa 
      tgtctctgc ggggaagtc ccaaatctt cattttgtt 
      tttacattg ggactactg aagggattc tctacaacg 
      ctttgaact cggaattcg ttaattttg agacaccct 
      gtaagtttc tccgttagt cactttttg tatatacta 
      gacgataag ttgccgtag ttaatattt aagctacta 
      tagtcccac gaaatgaat ttatctgtg cctggattc 
      actaaaaaa agtttaaaa attgaacta tttgttgtt 
      atttataac cccacaaac atgtttaac aataacata 
      ttacaaaat attacccca aggactcga atttgttac 
      gaaagttta ataagagtc cgagtgttt aacagcatt 
      tcgtcgccg tttttttac gatttaact tcgtaattt 
      tacattaaa cgtttttgt gagccaatt ttaataatt 
      tttgcgatc taggcggct gtgaatttc gtattaagg 
      aaatttgcc atcgttggg gtcattgaa cccaaaaat 
      tacgaccaa aagacacac attttcact aaatccata 
      ttagtttca ctcataatg tctttgata aaaattgaa 
      aaaaaaatt gtcttagaa gttaaccat taccacagc 
      cattactaa ccaaatttt gtacttact tgaaaaaca 
      ccgaagccg ccgtttttc tcaagacta accactcca 
      caccgaaac actcagtct tttcacatt tttctgtaa 
      atatttgtc caaaataca cgcattaac accttttct 
      tcgaattaa attgtggaa aatgcgacc accattaaa 
      tttgaccca ccgccaatt gtttcatta ccaacatta 
      atacaatta aatcactag atgtcacag cgcctaaat 
      caaactttt gaattttca attgcaaaa tactccgat 
      actatcgcc cgttatttc caagttttc cttacctca 
      atttgtaaa aaaaacacc agactcagt catttgctt 
      taatttcac taaaatcta caatatata caaacagtc 
      ttaaaataa tggtaccct tacattaac tgtaattat 
      gtaaacatg atttgaagt aaaaataaa tactttgat 
      aattt
    • >TC033602 RB
      cgtgacttg tcttctgat atttagtgg tgactttac 
      ggccggaaa atggacgag agaaaaatg gaaccgaag 
      gaagtttcc actgaagtt ttagttaaa gtgtattgc 
      gaaattgag tggtgtttt agtgactct ggggataat 
      aacatgcta attaagaaa atgaagtta tcggatggc 
      accgagtct ccaatatta gaagaacaa gagagtgcc 
      aactcagag aaaccaaaa caactcgtc aaatatgga 
      gaactagtc attttagga tacaacggt tttctacct 
      cagggcgac cgaggcaga cgacgtagc aaattcgta 
      ttgtacaaa cgaccaaca gcgaacgga gtcaagaga 
      tcgaaacac tatgtcgtc aaaacgccc cattcgtct 
      caggccatc ctggacgca aaccagcac tcgatatcc 
      tacacactt tcacgcaac catgccgtc atagtggaa 
      tacaccccc gacgatgac accgacatg tttcaaatc 
      ggtcgttcg tcggaagcc cccatcgac ttcgtggtc 
      atggacacc gtccccggg gacaagact ggcgaaacc 
      aaggtgatg caaagcacg atatcgcga tttgcgtgt 
      cggatttta tgcgatagg aagaatccg aaaattgcg 
      aggatatac gccgccggt tttgacacc tccaaaaac 
      atatttctc ggggagaaa gcaacgaaa tggcaggaa 
      aacgggcgc gaaatcgac ggtttgacc acgaatggt 
      gtgttaatt atgcatcca agagggacg ttttgtggg 
      ggagatgcc aagtgtagt tattggttg gagacgtct 
      gttggtgga ggtgtgttt tcgttgagg gagtcaagg 
      tcggcccaa cagaagggc caagtggtg gaaaacgag 
      acgaatatt ttggaagat gggactttg atcgatttg 
      tgcggggct acgctgttg tggaggtcg gcagagggg 
      ctcacaaaa tcgccgagc aaacgcgac ctggagcgc 
      gaaatcgac gaaatcaac gccggtcgc ccgcagtgc 
      cccgttggc ttgaacact ttagtcatt ccccgaaaa 
      gtcacaccg aacgaaaac ctgcaacag ccttacgtt 
      tatctcaac tgcggacac gtccaaggc ttgcacgat 
      tggggccaa gacaaggat tcgggcgcc cgaaagtgc 
      cccatttgc ctagaattg ggaccggtt gtgaaggtc 
      tttatgggg ctcgagccg gcgttttat gtcgattcg 
      gggcccccg acgttcgcg ttcaacccg tgcgggcac 
      atggccacc gaaaagacc gtcaagtac tgggcaaat 
      gtggctatt ccgcatggt acgaatggg tttcacgcc 
      gtgtgcccc ttttgcgct tcgcccctg gcggggtct 
      ccggggtac gtccgccta attttccag gataatgtg 
      gattgaaag gaatgtaga gtagtccac caaagcttt 
      atttatgtt tttgctctt ttatttaat gtataattg 
      gtttttagc agtctcatt tattaattt tttggaaat 
      tacccaatt gtaattact tgtaattat tgtatataa 
      ttgcacttg aagatggga gcgagccga gaattgcag 
      ttgtatcat atcatgatt tttttaatg gaaattgcg 
      gtttattac agtgcaaat gcgtgaatc gttaagact 
      ggttaggat ttagcttat aatgtaata agtatttaa 
      tataaatcg acctgttaa tgcaatgtt ttattcaaa 
      aaaaatatt tttacatac ttaattgac ccagatttt 
      ttgatttta tcgtgataa caggctgtc tcaaaattg 
      gcgaatttt agttcagag ctttgtaga gaattgctt 
      caatagtcc aaatataga aaaaaaaat taagattct 
      gggtttcaa ccaatgtct ctgcgggga agtcccaaa 
      tcttcattt tgtttttac attgggact actgaaggg 
      attctctac aacgctttg aactcggaa ttcgttaat 
      tttgagaca ccctgtaag tttctccgt tagtcactt 
      tttgtatat actagacga taagttgcc gtagttaat 
      atttaagct actatagtc ccacgaaat gaatttatc 
      tgtgcctgg attcactaa aaaaagttt aaaaattga 
      actatttgt tgttattta taaccccac aaacatgtt 
      taacaataa catattaca aaatattac cccaaggac 
      tcgaatttg ttacgaaag tttaataag agtccgagt 
      gtttaacag catttcgtc gccgttttt ttacgattt 
      aacttcgta attttacat taaacgttt ttgtgagcc 
      aattttaat aatttttgc gatctaggc ggctgtgaa 
      tttcgtatt aaggaaatt tgccatcgt tggggtcat 
      tgaacccaa aaattacga ccaaaagac acacatttt 
      cactaaatc catattagt ttcactcat aatgtcttt 
      gataaaaat tgaaaaaaa aattgtctt agaagttaa 
      ccattacca cagccatta ctaaccaaa ttttgtact 
      tacttgaaa aacaccgaa gccgccgtt tttctcaag 
      actaaccac tccacaccg aaacactca gtcttttca 
      catttttct gtaaatatt tgtccaaaa tacacgcat 
      taacacctt ttcttcgaa ttaaattgt ggaaaatgc 
      gaccaccat taaatttga cccaccgcc aattgtttc 
      attaccaac attaataca attaaatca ctagatgtc 
      acagcgcct aaatcaaac ttttgaatt ttcaattgc 
      aaaatactc cgatactat cgcccgtta tttccaagt 
      tttccttac ctcaatttg taaaaaaaa caccagact 
      cagtcattt gctttaatt tcactaaaa tctacaata 
      tatacaaac agtcttaaa ataatggta cccttacat 
      taactgtaa ttatgtaaa catgatttg aagtaaaaa 
      taaatactt tgataattt
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