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Gene information
General information
TC009671 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3815097..3816694
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009671
    iBeetle screen iB_04677

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009671 RA
      MKYVKLSHV NGLLEMPMV GLGTWRAQP EEMEKALWA 
      ALDAGYRHI DTAFNYNTE ETIGKVLKQ WFSQGKGKR 
      EDLFITTKL PNCANRACD VARFLQMSL KKLQTNYVD 
      LYLIHMPFS FHLNETNFT PLTNEDGSF SLDYDSDLI 
      ATWQAMEQQ VDQGLIKAI GLSNFSGEQ IERIYTAAR 
      IKPSVLQIE LHAYLQQNE LRALCRKLG IAVTAYAPL 
      GSPGANNHF NSKYNYSIH DFPDILGHP LVDQLAQKY 
      NKTPGQVLL RHLIQQDII VIPKSGNSE RLKSNIDVF 
      DFELSREDM EQLNGLDRG ENGRIFDFL FFKGVEKHP 
      EYPFKHRID GW
    CDS sequence
    • >TC009671 RA
      atgaaatac gtaaaattg tcacacgtt aatgggctt 
      ctagaaatg cccatggtg ggtctgggc acgtggagg 
      gcccagcca gaggagatg gaaaaggct ttatgggcc 
      gccttggac gctggctat agacacatt gacactgct 
      tttaattat aatacggag gaaacgatc ggaaaagta 
      ctaaaacag tggtttagt cagggcaaa ggcaagcga 
      gaggatcta ttcataacg actaaatta ccaaattgt 
      gcaaaccgg gcttgtgac gtcgcccgt ttcttacaa 
      atgtccctc aaaaaacta caaacaaat tacgtggac 
      ttgtaccta atccacatg cccttcagc ttccactta 
      aacgagaca aacttcaca cctttgacg aacgaagac 
      ggttcgttt agtctcgac tacgacagt gatttaatc 
      gcaacctgg caggcgatg gaacaacaa gtcgatcaa 
      ggcttaatc aaagcgatc ggtttatca aacttcagt 
      ggcgaacaa atcgaacga atttacacc gctgcgcgg 
      atcaaacct tccgtttta caaatcgaa ctgcacgct 
      tacttgcaa caaaacgaa ctgcgggcg ctttgccgc 
      aaattgggg atcgcagtg acggcgtac gcgcccctg 
      ggaagcccc ggcgccaac aaccatttc aattcgaaa 
      tacaattac tcgattcac gactttccc gatattttg 
      ggccacccg ctcgtggac caactggca caaaagtac 
      aataaaacc ccaggccag gtcttgctg cggcacttg 
      atccagcag gatattatt gtgataccg aaaagtggt 
      aattcggag cgactcaag tcaaacatt gacgtgttc 
      gattttgag ttgagtcgg gaggatatg gaacagttg 
      aatggactg gatcggggg gagaatggg cgaattttc 
      gacttcttg tttttcaaa ggagtggag aaacatccc 
      gagtatcca ttcaaacac cggatcgac ggttggtag
    mRNA sequence
    • >TC009671 RA
      aaaatcgat aagtttcta accctctca atcgcgcca 
      taaaataca aacaagtga aaatttgac aatctaaaa 
      atagtgtca agttcgcat gaaaatagc gtctcaacc 
      taattacaa acttcagta gtaaacgaa acggcgaac 
      tatgaaata cgtaaaatt gtcacacgt taatgggct 
      tctagaaat gcccatggt gggtctggg cacgtggag 
      ggcccagcc agaggagat ggaaaaggc tttatgggc 
      cgccttgga cgctggcta tagacacat tgacactgc 
      ttttaatta taatacgga ggaaacgat cggaaaagt 
      actaaaaca gtggtttag tcagggcaa aggcaagcg 
      agaggatct attcataac gactaaatt accaaattg 
      tgcaaaccg ggcttgtga cgtcgcccg tttcttaca 
      aatgtccct caaaaaact acaaacaaa ttacgtgga 
      cttgtacct aatccacat gcccttcag cttccactt 
      aaacgagac aaacttcac acctttgac gaacgaaga 
      cggttcgtt tagtctcga ctacgacag tgatttaat 
      cgcaacctg gcaggcgat ggaacaaca agtcgatca 
      aggcttaat caaagcgat cggtttatc aaacttcag 
      tggcgaaca aatcgaacg aatttacac cgctgcgcg 
      gatcaaacc ttccgtttt acaaatcga actgcacgc 
      ttacttgca acaaaacga actgcgggc gctttgccg 
      caaattggg gatcgcagt gacggcgta cgcgcccct 
      gggaagccc cggcgccaa caaccattt caattcgaa 
      atacaatta ctcgattca cgactttcc cgatatttt 
      gggccaccc gctcgtgga ccaactggc acaaaagta 
      caataaaac cccaggcca ggtcttgct gcggcactt 
      gatccagca ggatattat tgtgatacc gaaaagtgg 
      taattcgga gcgactcaa gtcaaacat tgacgtgtt 
      cgattttga gttgagtcg ggaggatat ggaacagtt 
      gaatggact ggatcgggg ggagaatgg gcgaatttt 
      cgacttctt gtttttcaa aggagtgga gaaacatcc 
      cgagtatcc attcaaaca ccggatcga cggttggta 
      gaattagta ttgttacaa agacaacaa taaattaac 
      tgatacgat acaacattt ttcgttgca tcctgtgct 
      gtaattgtc cacgcctga tcatcgtat agatgactt 
      tgtttatgg cttgttcag ggtactgct gttgggtga 
      agtcctgaa tgatggtct ggtgggaga cttgagtac 
      cgatagcaa accggcgtt ttagctgtt tttcgatgc 
      ggatcaaca tttcgtgct cttcttcag tcgtgaacc 
      cttcaccac ctggca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY