Status
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Gene information
General information
TC033599
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009666,TC009667
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3791857..3801204
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033599
    iBeetle screen iB_04676

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033599 RA
      MTDLKKKSL KVVETTVLE RLQWICNTV FHQLVAVAG 
      TFILWTLCY NFQNKLSTW HMILTTIAY LPVMAEAIV 
      LFASDNVWT LNIQRTTKY YIHGCLLFC SAVLATVGI 
      AFMINVHSN PHFHSTHAW TGLVSWILV LLSQFLGIA 
      SAKSQDLKN ILRPVWFKF LHNLFGMLG YIFGLVSLC 
      YGLDKHSIK NATSEESRL ATVVMIGVI GAWSIIAAL 
      KSGIGQLRA IFSEKMSSE SVTHIQRLS WIFNVLFHQ 
      ALAVVTVYI LCTAFVNGD IDSLSTWHM VLTPLGFLF 
      LMMESFILF TNTNIHTLN LERTKKQYV HTSLQFLST 
      VCITVGISF KISSKNGDH FTSTHAILG LVAWILVWC 
      SVLLGLASS QSQLLKKIA KPVVLKFLH NLIGICAFT 
      FGIITLCYG LKEYFKWVS REDTINAAV VTIALLYLW 
      ALFDPFSVL FPGKYRLEL IQTMANFGS LAKNSVRFL 
      LNYLARSCL SGVVIYCCW ISIMAYSWF TWHVILCTL 
      GMIPLMSEA LFLFASDEL WSKQLTRTR KYFIHGFLM 
      TLGTVLVTA GCIDTFCFI TEGYHLYTA HGITGLVAM 
      ILMIVSLVL GAMANYSND LRKYARPVT FKFNHNFVG 
      LVGYIIGIV SLCFSFYTN WFCFFTSYE SRIVALVAT 
      GLGTLWTMN PALVSGKHQ VHQTSHKCN KMKLKLLLF 
      LLYFAKVKT TEPENLKKP VSGFLYSMP KVLQAGRNE 
      TVCLSLHSS SFTPIKITV DLKIKQAHF YTNRIIDSE 
      HTCFEMHVP VNTKNEAQF ASVRVEIQT NGTVLSAHN 
      QDPIVIYPN KKITFIETD RMTYKSKDT VRLRVLTLG 
      NNLLPILTH KIPFVRIRN PLGVGVIVW ENVTTELGL 
      AQLEYQLPQ DPIEGKWKV EIGEDFRAF EVSKYVLPR 
      FKVQILHPK IIYIGSRVA IKVCGRYSY GEMVKGSAF 
      IRLSSIFPN FKTFQSLKK MDEGCAEFV LTPTDFSYF 
      SIKKLFPLS DPKISVLIT ATVTEHGTD KIELDATKS 
      VISLKPYGL KFAKKAMFM PGLPYQGFL QLNNVNMDL 
      RGQVIEICY NIAIKKSWN YLNNEQCSN FTLQGNEKL 
      IPFHILPLK NNVIHLQLN ARSLNFTNI VDNFLVVRL 
      FSPSLTYIT IDQIHHSNN CKSIQQFAV QYTTEKLKE 
      HENVTFFYM IKSRGQIFK LRKITHNVR KSSPNYSTE 
      FKDILGASH KHTKASSIA KFTLKFKLD EKIFSNYQL 
      LIYYVTPEG ELASANKEV EVEPCLNNK VEANWSHKQ 
      IAPGATASL LIESKSESL CSVVTTDKA VTFMDDLRF 
      LNVKSLLKP FLQQKEAPE SGRKSCLPP VKKNRRRRF 
      VYSFSEDFD AYDIFEKFG IVTITNFKV VTKPCYTGP 
      IPPTEDPVS SLTDQYDTQ NEDNITPIR SFFPETWLW 
      EIVPVRSVA VIHRTLPHT ITTWMTNVM CVSATEGVG 
      FSKTGEITT FRPFFVDIL TPYSIKRGE TLYLHAIIF 
      NYLTYNIPI RITLGTSEG LKLVDTKNR KSFSYCISS 
      NNTATHIFE LKGTDVGNV NITVVAELD PNFPGHCGP 
      EIIINKRDV VFKTLIVEP EGHPITVTK SALLCATDN 
      ITWELPVPN DVVAKTANS KLILNGDIL GQTIQNLDD 
      LIAMPTGCG EQIMANLAP NIYILKYLN ETKQLTSSV 
      RHKIARNLK IGYQRILNY IHKDGSFSA FGYHDSSGS 
      MFLTAFVVR TLQEMKKLV YVDQKIIER AVLWILSHQ 
      LENGCFSTM SHVFQDMGG TNSENSTAA LTAYVIISL 
      LDGNIDVPE AVKTNAKYC IRGYYDLDR YTLAISSYA 
      LLKINWFSE AERMLKKLF QVSSHKDNM MWWTNREIN 
      GSEASDIEV TSYVLLALI QQKNEENLA KAHSIVQWL 
      STKLGHRGS FKTTQDTVV ALDALTKYS KFLSHKTDI 
      NINVVALES AHNFVMTDK DRLKSKKIV LKNPANKVR 
      VEVQGQGCV LIQAITSYN VKQLRNGDA FKLDMEVLP 
      VSNIDKCSI TTLSPCFKY NGPDHIANM AILEVGLPS 
      GYQADRASL YKLIDESSV KMFEELEEK IVLYLTKLG 
      NRQMCVNFN INENAIVKS RSNSTVKLY DYYKPEYQV 
      SQFYKIKEN CTVNSTIPA ISQNVSTPI EDKRKKLSK 
      R
    CDS sequence
    • >TC033599 RA
      atgaccgat ttaaaaaag aaaagtttg aaagtggtg 
      gaaaccacg gttttggaa cggctccaa tggatttgt 
      aacacggtt tttcaccag ttggtcgca gtggccggg 
      acttttatt ttgtggact ctgtgctac aacttccaa 
      aataaacta tcaacatgg cacatgatt ctaacaacg 
      attgcttat cttcctgtg atggccgaa gccatcgtt 
      ctcttcgcc agcgacaac gtctggacc ttaaacatc 
      caaagaacc acgaagtac tacatccac ggttgtctc 
      ctcttctgt agcgccgtt ttggcaacc gttggtatc 
      gcatttatg ataaatgtc cactcgaac cctcacttt 
      cactcaacc cacgcttgg acaggtttg gtgtcgtgg 
      attttggtc ctgttgtca caattcctc ggcatcgcc 
      tcggccaaa tcccaggat ttgaagaac attctccgg 
      cccgtttgg ttcaaattt ctgcacaac ttgttcgga 
      atgttgggc tatattttc ggtctggtc agtttgtgt 
      tacgggtta gacaaacac tcgattaaa aatgcaaca 
      agtgaggag tcaagactt gccactgtt gtcatgatt 
      ggggtcatt ggggcgtgg agcattatt gcggccctt 
      aagtcagga attgggcaa ctaagggcc attttttct 
      gaaaaaatg tcgagcgaa agtgtcacc cacatccag 
      agactttcc tggattttc aacgtcctg ttccaccag 
      gctttagca gtggtcact gtgtatatt ctgtgcacg 
      gctttcgtc aacggagac atcgattca ctatcgaca 
      tggcacatg gtcctaaca ccgcttggc tttttgttc 
      ctgatgatg gaaagcttc attttattc acaaacacc 
      aacatccat actttaaac ttggagcga accaaaaaa 
      caatacgtc cacaccagt ctccagttc ctcagcact 
      gtctgtatc acggttgga atatccttc aagattagc 
      tcaaaaaac ggcgatcat ttcacctca actcacgca 
      attttgggt ctagttgcc tggatcctg gtctggtgt 
      tcagtcctt ctgggcctg gcttcgtcc caatcccaa 
      cttttgaaa aaaatcgcc aaaccagtg gtcctcaaa 
      tttttgcac aacttgatc gggatttgt gcctttacg 
      ttcggaatc attacattg tgttacggc cttaaggag 
      tacttcaag tgggtttca cgcgaagat acgatcaat 
      gcagctgtc gtaacaatc gcccttttg tacctttgg 
      gcccttttt gaccccttc agtgtgtta ttccccggg 
      aaatatcgt cttgagtta attcagaca atggctaac 
      ttcggttcg ctcgcaaag aattcggtc cgatttttg 
      ctcaattat ttagcgagg agttgttta tcaggtgtt 
      gttatttat tgttgttgg atcagtatt atggcgtat 
      tcgtggttc acctggcac gtgattttg tgcacactt 
      gggatgatt ccactgatg agcgaagct ttgttcctt 
      ttcgcctcc gacgagctc tggtccaaa caactaacc 
      cgtactcgg aaatacttc attcatggc tttttgatg 
      accttagga acggtttta gtgactgct ggttgcatc 
      gacactttt tgttttatc acagagggt tatcacttg 
      tacacagcg cacgggatc acaggttta gttgcaatg 
      atcttgatg attgtttcg ctcgttttg ggggccatg 
      gcaaattat tcgaatgat ttaaggaaa tacgcaaga 
      ccggtcact ttcaagttc aatcataat tttgtcggt 
      ctggtggga tacatcatc gggatagtt agtttgtgt 
      ttttcgttt tacaccaat tggttttgc ttttttacc 
      tcgtacgag tctagaatt gtagcgctg gttgccacc 
      ggtttgggg actctgtgg acgatgaat ccggccctg 
      gtttccgga aaacatcaa gtgcaccaa actagtcac 
      aagtgcaac aaaatgaaa ctcaaactc cttttattt 
      cttctctat ttcgccaaa gtcaaaaca accgaaccc 
      gaaaatttg aaaaaaccc gtctcggga ttcttgtat 
      agcatgcca aaagttttg caagccgga agaaatgaa 
      acggtttgt ttatcactc cattcgtca tccttcaca 
      ccgattaaa ataaccgtg gatttgaaa atcaaacaa 
      gcacatttt tacacaaac agaatcata gattcagaa 
      cacacatgc ttcgaaatg cacgtgccc gtgaacaca 
      aaaaacgaa gcccaattt gcaagtgtc agggttgaa 
      atacaaaca aacgggacc gtgctctca gcccataat 
      caggacccc atcgtcatt taccccaac aagaaaatt 
      acgttcatt gaaaccgat cgaatgacg tacaaatcc 
      aaagacacg gtcaggttg agagtacta acactggga 
      aataatttg ctaccgatt ttaacacac aaaataccg 
      tttgttcga atccggaac ccactgggt gtgggggtg 
      atagtctgg gaaaacgtc accacggag ctggggctg 
      gcccagctg gagtaccaa ctcccccaa gacccgatt 
      gagggcaaa tggaaagta gaaatcggt gaagatttt 
      cgcgccttt gaggtcagc aaatatgtg cttccccga 
      tttaaggtc cagatttta cacccgaaa ataatttac 
      atcgggtca cgtgtcgca attaaagtg tgtggccga 
      tattcgtac ggagaaatg gtcaaagga agtgctttt 
      atccgcctg tcgagtatt ttccccaac tttaagact 
      tttcagtca ctgaaaaaa atggatgag ggttgtgcc 
      gaatttgtg ctaactccg accgatttt tcctacttc 
      agcatcaaa aaattgttc ccgttgagt gacccgaaa 
      attagtgtt ttgatcaca gcaacggtc actgaacac 
      gggactgat aaaattgag ttagatgct accaaaagt 
      gtgatttcg ctaaagcct tacggcctc aagtttgcg 
      aaaaaagcc atgttcatg ccaggattg ccgtaccaa 
      ggattcctc caactgaac aatgttaat atggacttg 
      aggggtcaa gtgattgag atttgttac aacatcgcg 
      attaagaaa agttggaat tatttaaac aacgagcaa 
      tgttccaat tttacgctg caagggaat gaaaaactg 
      atccccttc catatttta cccttgaaa aataacgtg 
      atacatttg cagttaaat gcgcgatcg ctgaatttc 
      acaaacatc gtcgataac ttcctagtg gtgcgatta 
      ttttcgccc agtttgacc tacatcacg attgaccaa 
      atccaccat tccaacaat tgtaaatcc attcagcaa 
      ttcgcagtg caatacacg accgaaaaa ttgaaagag 
      cacgaaaat gtgactttt ttttacatg attaaatca 
      cgaggccag atcttcaaa ttgagaaaa atcacacat 
      aacgtgcgg aaatcgtcc ccgaactac tccaccgaa 
      ttcaaggat atcctagga gcgagccac aaacacacc 
      aaagcgagc tcaatcgcc aaattcacg ctgaaattc 
      aaactggac gagaaaatc ttctccaac taccaactc 
      ctgatttac tacgtaaca cccgagggc gaattagct 
      tcggctaat aaagaagtc gaggtcgag ccttgcctc 
      aacaataag gtcgaagca aactggtcc cacaaacaa 
      atcgctccg ggagcaacc gcctcgcta ctaatcgaa 
      agcaaatcc gagtcactt tgctcggtt gtaaccacc 
      gataaggcg gttacgttc atggacgat ttgcgtttc 
      ctgaacgta aaatcactg cttaagccg ttcttgcag 
      caaaaggag gcgccagaa tcgggtcga aaatcgtgt 
      cttcccccc gtgaagaaa aaccgcagg agacgattc 
      gtttactcg ttttcggag gatttcgac gcttacgat 
      atttttgaa aaattcggg attgtgacc attacgaat 
      tttaaggtc gtcacaaag ccctgttac acaggacct 
      attccgcca actgaagac ccagtttct tcactcact 
      gatcagtac gacacgcaa aatgaagac aacatcact 
      ccaatcagg tcctttttt ccagagacg tggctctgg 
      gaaattgtc ccagtcagg agtgtggca gtgatccac 
      cgcactcta cctcacact atcaccact tggatgacc 
      aatgtgatg tgtgtttcg gccactgaa ggcgttggg 
      tttagtaaa accggcgaa attacaacg tttcgacca 
      tttttcgtg gacatccta acaccatat tcgatcaaa 
      agaggggaa actttgtac ctacatgcc attatcttc 
      aattatctg acttacaac atcccgatt cgcataact 
      ttgggaact agtgagggt ctcaaacta gtcgataca 
      aaaaaccgg aaaagcttc tcttactgc atttcgagc 
      aacaataca gcgacacac attttcgaa ttgaaggga 
      actgacgtc gggaatgtt aatattact gtggtggca 
      gagctggac ccgaatttc cccggccat tgcggcccc 
      gaaatcata atcaataaa agagacgtc gtttttaaa 
      acactgata gtcgagccc gaagggcac ccaatcact 
      gtgaccaaa tcggcactt ttatgtgcc acagacaat 
      atcacgtgg gagcttcca gtcccgaat gacgttgtt 
      gccaaaacg gccaactcg aaactgatc ctcaacggt 
      gatattttg ggccaaaca atccaaaac ttggacgac 
      ttgattgca atgccaacg ggatgcggt gaacaaatc 
      atggccaac ttggccccc aatatatac attttaaaa 
      tatctaaac gaaaccaaa caactaaca tcgagtgtg 
      cggcacaaa atcgcgcgg aatttgaaa atcggctac 
      caaagaatt ctgaattat attcacaaa gacggctcg 
      ttttcagcg ttcgggtac cacgattct tcaggttcg 
      atgtttttg acggctttt gtggtccgg acgttgcaa 
      gaaatgaaa aaattggtt tacgtcgac cagaaaata 
      atcgagcgg gctgtgtta tggattttg agccaccag 
      ctcgaaaac ggctgtttt agcaccatg tcgcacgta 
      tttcaggat atgggcggc actaatagt gagaatagc 
      acggctgct ctcacagcc tacgtgata attagcctt 
      ctggatggc aatattgac gttcctgaa gcggtgaaa 
      actaacgca aaatattgc attcgcggg tattatgac 
      cttgatagg tacacccta gccataagt tcgtacgca 
      cttttgaaa ataaattgg ttcagtgag gcggaacga 
      atgctgaaa aaactgttc caagtgtcg agtcataaa 
      gacaacatg atgtggtgg actaatcga gaaatcaac 
      gggtccgag gcaagtgat attgaagtc acaagttac 
      gtcctcctg gctttaatt cagcaaaaa aacgaggaa 
      aatttagcc aaagctcac tcgatcgtc cagtggtta 
      tccacaaag ctaggacat cgaggaagt ttcaaaact 
      acccaagac acggttgtg gctttggac gcattaaca 
      aaatattcc aaatttttg agtcacaaa accgacata 
      aatatcaac gttgtggcc ctagaatcg gcgcacaat 
      ttcgtaatg actgacaaa gatcggctc aaatctaaa 
      aaaatcgtt ttgaaaaat cccgcaaat aaagtgcga 
      gttgaggtc caaggacaa gggtgtgtt ttaatacaa 
      gcgataact tcgtataat gtcaagcag ttaagaaat 
      ggtgatgct tttaagctc gatatggag gtcttgccg 
      gtgtcaaat attgacaag tgttcaatt acaacttta 
      tctccctgt tttaagtac aatggacca gaccacatt 
      gcaaatatg gccattctg gaagtgggt cttccctca 
      ggataccaa gccgatcgg gcgtcactt tacaaactt 
      atcgacgag tcaagcgtg aaaatgttt gaagagttg 
      gaagaaaaa atcgtgctg tacttaaca aaactcgga 
      aaccgccaa atgtgtgtc aatttcaac atcaacgaa 
      aatgccatt gtcaaatca cgatccaac tcaactgtt 
      aaactttac gattattac aaaccggag tatcaagtg 
      tcacaattt tacaaaatc aaggaaaat tgcacagtg 
      aatagcaca attcctgcc atttcgcaa aatgtaagc 
      acgccgatt gaagataaa aggaagaaa ttgtccaag 
      agataa
    mRNA sequence
    • >TC033599 RA
      ataacaatg accgattta aaaaagaaa agtttgaaa 
      gtggtggaa accacggtt ttggaacgg ctccaatgg 
      atttgtaac acggttttt caccagttg gtcgcagtg 
      gccgggact tttattttg tggactctg tgctacaac 
      ttccaaaat aaactatca acatggcac atgattcta 
      acaacgatt gcttatctt cctgtgatg gccgaagcc 
      atcgttctc ttcgccagc gacaacgtc tggacctta 
      aacatccaa agaaccacg aagtactac atccacggt 
      tgtctcctc ttctgtagc gccgttttg gcaaccgtt 
      ggtatcgca tttatgata aatgtccac tcgaaccct 
      cactttcac tcaacccac gcttggaca ggtttggtg 
      tcgtggatt ttggtcctg ttgtcacaa ttcctcggc 
      atcgcctcg gccaaatcc caggatttg aagaacatt 
      ctccggccc gtttggttc aaatttctg cacaacttg 
      ttcggaatg ttgggctat attttcggt ctggtcagt 
      ttgtgttac gggttagac aaacactcg attaaaaat 
      gcaacaagt gaggagtca agacttgcc actgttgtc 
      atgattggg gtcattggg gcgtggagc attattgcg 
      gcccttaag tcaggaatt gggcaacta agggccatt 
      ttttctgaa aaaatgtcg agcgaaagt gtcacccac 
      atccagaga ctttcctgg attttcaac gtcctgttc 
      caccaggct ttagcagtg gtcactgtg tatattctg 
      tgcacggct ttcgtcaac ggagacatc gattcacta 
      tcgacatgg cacatggtc ctaacaccg cttggcttt 
      ttgttcctg atgatggaa agcttcatt ttattcaca 
      aacaccaac atccatact ttaaacttg gagcgaacc 
      aaaaaacaa tacgtccac accagtctc cagttcctc 
      agcactgtc tgtatcacg gttggaata tccttcaag 
      attagctca aaaaacggc gatcatttc acctcaact 
      cacgcaatt ttgggtcta gttgcctgg atcctggtc 
      tggtgttca gtccttctg ggcctggct tcgtcccaa 
      tcccaactt ttgaaaaaa atcgccaaa ccagtggtc 
      ctcaaattt ttgcacaac ttgatcggg atttgtgcc 
      tttacgttc ggaatcatt acattgtgt tacggcctt 
      aaggagtac ttcaagtgg gtttcacgc gaagatacg 
      atcaatgca gctgtcgta acaatcgcc cttttgtac 
      ctttgggcc ctttttgac cccttcagt gtgttattc 
      cccgggaaa tatcgtctt gagttaatt cagacaatg 
      gctaacttc ggttcgctc gcaaagaat tcggtccga 
      tttttgctc aattattta gcgaggagt tgtttatca 
      ggtgttgtt atttattgt tgttggatc agtattatg 
      gcgtattcg tggttcacc tggcacgtg attttgtgc 
      acacttggg atgattcca ctgatgagc gaagctttg 
      ttccttttc gcctccgac gagctctgg tccaaacaa 
      ctaacccgt actcggaaa tacttcatt catggcttt 
      ttgatgacc ttaggaacg gttttagtg actgctggt 
      tgcatcgac actttttgt tttatcaca gagggttat 
      cacttgtac acagcgcac gggatcaca ggtttagtt 
      gcaatgatc ttgatgatt gtttcgctc gttttgggg 
      gccatggca aattattcg aatgattta aggaaatac 
      gcaagaccg gtcactttc aagttcaat cataatttt 
      gtcggtctg gtgggatac atcatcggg atagttagt 
      ttgtgtttt tcgttttac accaattgg ttttgcttt 
      tttacctcg tacgagtct agaattgta gcgctggtt 
      gccaccggt ttggggact ctgtggacg atgaatccg 
      gccctggtt tccggaaaa catcaagtg caccaaact 
      agtcacaag tgcaacaaa atgaaactc aaactcctt 
      ttatttctt ctctatttc gccaaagtc aaaacaacc 
      gaacccgaa aatttgaaa aaacccgtc tcgggattc 
      ttgtatagc atgccaaaa gttttgcaa gccggaaga 
      aatgaaacg gtttgttta tcactccat tcgtcatcc 
      ttcacaccg attaaaata accgtggat ttgaaaatc 
      aaacaagca catttttac acaaacaga atcatagat 
      tcagaacac acatgcttc gaaatgcac gtgcccgtg 
      aacacaaaa aacgaagcc caatttgca agtgtcagg 
      gttgaaata caaacaaac gggaccgtg ctctcagcc 
      cataatcag gaccccatc gtcatttac cccaacaag 
      aaaattacg ttcattgaa accgatcga atgacgtac 
      aaatccaaa gacacggtc aggttgaga gtactaaca 
      ctgggaaat aatttgcta ccgatttta acacacaaa 
      ataccgttt gttcgaatc cggaaccca ctgggtgtg 
      ggggtgata gtctgggaa aacgtcacc acggagctg 
      gggctggcc cagctggag taccaactc ccccaagac 
      ccgattgag ggcaaatgg aaagtagaa atcggtgaa 
      gattttcgc gcctttgag gtcagcaaa tatgtgctt 
      ccccgattt aaggtccag attttacac ccgaaaata 
      atttacatc gggtcacgt gtcgcaatt aaagtgtgt 
      ggccgatat tcgtacgga gaaatggtc aaaggaagt 
      gcttttatc cgcctgtcg agtattttc cccaacttt 
      aagactttt cagtcactg aaaaaaatg gatgagggt 
      tgtgccgaa tttgtgcta actccgacc gatttttcc 
      tacttcagc atcaaaaaa ttgttcccg ttgagtgac 
      ccgaaaatt agtgttttg atcacagca acggtcact 
      gaacacggg actgataaa attgagtta gatgctacc 
      aaaagtgtg atttcgcta aagccttac ggcctcaag 
      tttgcgaaa aaagccatg ttcatgcca ggattgccg 
      taccaagga ttcctccaa ctgaacaat gttaatatg 
      gacttgagg ggtcaagtg attgagatt tgttacaac 
      atcgcgatt aagaaaagt tggaattat ttaaacaac 
      gagcaatgt tccaatttt acgctgcaa gggaatgaa 
      aaactgatc cccttccat attttaccc ttgaaaaat 
      aacgtgata catttgcag ttaaatgcg cgatcgctg 
      aatttcaca aacatcgtc gataacttc ctagtggtg 
      cgattattt tcgcccagt ttgacctac atcacgatt 
      gaccaaatc caccattcc aacaattgt aaatccatt 
      cagcaattc gcagtgcaa tacacgacc gaaaaattg 
      aaagagcac gaaaatgtg acttttttt tacatgatt 
      aaatcacga ggccagatc ttcaaattg agaaaaatc 
      acacataac gtgcggaaa tcgtccccg aactactcc 
      accgaattc aaggatatc ctaggagcg agccacaaa 
      cacaccaaa gcgagctca atcgccaaa ttcacgctg 
      aaattcaaa ctggacgag aaaatcttc tccaactac 
      caactcctg atttactac gtaacaccc gagggcgaa 
      ttagcttcg gctaataaa gaagtcgag gtcgagcct 
      tgcctcaac aataaggtc gaagcaaac tggtcccac 
      aaacaaatc gctccggga gcaaccgcc tcgctacta 
      atcgaaagc aaatccgag tcactttgc tcggttgta 
      accaccgat aaggcggtt acgttcatg gacgatttg 
      cgtttcctg aacgtaaaa tcactgctt aagccgttc 
      ttgcagcaa aaggaggcg ccagaatcg ggtcgaaaa 
      tcgtgtctt ccccccgtg aagaaaaac cgcaggaga 
      cgattcgtt tactcgttt tcggaggat ttcgacgct 
      tacgatatt tttgaaaaa ttcgggatt gtgaccatt 
      acgaatttt aaggtcgtc acaaagccc tgttacaca 
      ggacctatt ccgccaact gaagaccca gtttcttca 
      ctcactgat cagtacgac acgcaaaat gaagacaac 
      atcactcca atcaggtcc ttttttcca gagacgtgg 
      ctctgggaa attgtccca gtcaggagt gtggcagtg 
      atccaccgc actctacct cacactatc accacttgg 
      atgaccaat gtgatgtgt gtttcggcc actgaaggc 
      gttgggttt agtaaaacc ggcgaaatt acaacgttt 
      cgaccattt ttcgtggac atcctaaca ccatattcg 
      atcaaaaga ggggaaact ttgtaccta catgccatt 
      atcttcaat tatctgact tacaacatc ccgattcgc 
      ataactttg ggaactagt gagggtctc aaactagtc 
      gatacaaaa aaccggaaa agcttctct tactgcatt 
      tcgagcaac aatacagcg acacacatt ttcgaattg 
      aagggaact gacgtcggg aatgttaat attactgtg 
      gtggcagag ctggacccg aatttcccc ggccattgc 
      ggccccgaa atcataatc aataaaaga gacgtcgtt 
      tttaaaaca ctgatagtc gagcccgaa gggcaccca 
      atcactgtg accaaatcg gcactttta tgtgccaca 
      gacaatatc acgtgggag cttccagtc ccgaatgac 
      gttgttgcc aaaacggcc aactcgaaa ctgatcctc 
      aacggtgat attttgggc caaacaatc caaaacttg 
      gacgacttg attgcaatg ccaacggga tgcggtgaa 
      caaatcatg gccaacttg gcccccaat atatacatt 
      ttaaaatat ctaaacgaa accaaacaa ctaacatcg 
      agtgtgcgg cacaaaatc gcgcggaat ttgaaaatc 
      ggctaccaa agaattctg aattatatt cacaaagac 
      ggctcgttt tcagcgttc gggtaccac gattcttca 
      ggttcgatg tttttgacg gcttttgtg gtccggacg 
      ttgcaagaa atgaaaaaa ttggtttac gtcgaccag 
      aaaataatc gagcgggct gtgttatgg attttgagc 
      caccagctc gaaaacggc tgttttagc accatgtcg 
      cacgtattt caggatatg ggcggcact aatagtgag 
      aatagcacg gctgctctc acagcctac gtgataatt 
      agccttctg gatggcaat attgacgtt cctgaagcg 
      gtgaaaact aacgcaaaa tattgcatt cgcgggtat 
      tatgacctt gataggtac accctagcc ataagttcg 
      tacgcactt ttgaaaata aattggttc agtgaggcg 
      gaacgaatg ctgaaaaaa ctgttccaa gtgtcgagt 
      cataaagac aacatgatg tggtggact aatcgagaa 
      atcaacggg tccgaggca agtgatatt gaagtcaca 
      agttacgtc ctcctggct ttaattcag caaaaaaac 
      gaggaaaat ttagccaaa gctcactcg atcgtccag 
      tggttatcc acaaagcta ggacatcga ggaagtttc 
      aaaactacc caagacacg gttgtggct ttggacgca 
      ttaacaaaa tattccaaa tttttgagt cacaaaacc 
      gacataaat atcaacgtt gtggcccta gaatcggcg 
      cacaatttc gtaatgact gacaaagat cggctcaaa 
      tctaaaaaa atcgttttg aaaaatccc gcaaataaa 
      gtgcgagtt gaggtccaa ggacaaggg tgtgtttta 
      atacaagcg ataacttcg tataatgtc aagcagtta 
      agaaatggt gatgctttt aagctcgat atggaggtc 
      ttgccggtg tcaaatatt gacaagtgt tcaattaca 
      actttatct ccctgtttt aagtacaat ggaccagac 
      cacattgca aatatggcc attctggaa gtgggtctt 
      ccctcagga taccaagcc gatcgggcg tcactttac 
      aaacttatc gacgagtca agcgtgaaa atgtttgaa 
      gagttggaa gaaaaaatc gtgctgtac ttaacaaaa 
      ctcggaaac cgccaaatg tgtgtcaat ttcaacatc 
      aacgaaaat gccattgtc aaatcacga tccaactca 
      actgttaaa ctttacgat tattacaaa ccggagtat 
      caagtgtca caattttac aaaatcaag gaaaattgc 
      acagtgaat agcacaatt cctgccatt tcgcaaaat 
      gtaagcacg ccgattgaa gataaaagg aagaaattg 
      tccaagaga taatggccg ggaaaaaag tcccaccaa 
      tttattcac atttatttt tgtacttac caaaagtta 
      tgaactgaa aatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY