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Gene information
General information
TC009653 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3615178..3620675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009653
    iBeetle screen iB_04672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009653 RA
      MSHTSNEIE VKSWLAKIL KNADNFSVK IEGNSEKGD 
      GYLGDILFV QVTENTAKK HNLVLKCAK KSKLLREKS 
      PVQEAYLNE ILFYETVYP DFVRFQRER GVENGFDNV 
      VKCYGTLIS GDMEIIAMD NLKKKGYVL WDRKIPLTR 
      KHIDLVASA YGKLHAISI AMRDQEPQR FESLAKKPF 
      DFFVKFMKM SDMITTMER NFDEITDLM AGELEDGVL 
      RKWKDSKRR IRPFWENLR EIVTGMKVL THGDCWNNN 
      FMHYHINGE PADFVMIDW QLIYLTSPI YDLSYFLFA 
      CISEEDIND LDKILQLYH SSLRDHLKK LGSNPDLYP 
      FEDLVNDWK KYSFYGVII TNIIHKFVQ SDKDEVPDL 
      VDSVESGED MADVFNLPI KNTEDYKKR RLYITKYVV 
      EQEMSTQLE ANIKQWLAK ALKSQNLSD FSVKLVGNS 
      GKGDGYVGD ITFVQLSTP HQTYNLVVK CSKQSKVFT 
      RIFLREIFF YDTLLPTFI KFQKERNLA EIFGSVAKC 
      YGTYTSENI HVIILEDLK KLGYELWPV SKPMNREHI 
      EMVVREYGK FHATSVALK ILKPEKFQE ILATLSRTV 
      SDMFRTPEM QSIVRKSLK KAIELIEEE VAEEIVTKL 
      KHLVEKIQN TTLEFEENS RLNVVCHGG CWNNNYMYK 
      YDGSTKNVP SKLVIIDWQ IATCGTPIA DLSYFLFSY 
      ISAKDIEHL EDLLLVYHQ SFSNHLEKF GLIPENLYP 
      REQFLNDWQ ACSKYGLVL AIRSVRTSF IEKDEIVDL 
      EEALQQGVE LADYFTRDI RNMKGYKSR ITYLVNSK
    CDS sequence
    • >TC009653 RA
      atgtcccac actagcaac gagatcgag gtaaaatcg 
      tggttggcc aaaattttg aaaaacgct gataacttc 
      tcggtgaaa attgaggga aactcggaa aagggtgat 
      ggctacctt ggagacatc cttttcgtc caagtgact 
      gaaaacacc gccaaaaaa cacaatctc gtgctaaaa 
      tgtgcaaag aagagtaaa ctcctgcgc gagaaatcg 
      ccagttcag gaggcttat ttgaacgaa attttgttc 
      tacgaaact gtttacccc gattttgtg cgttttcaa 
      agggagaga ggtgtggag aacgggttc gataacgtg 
      gtaaagtgt tacgggacg ttgatcagc ggcgatatg 
      gaaataatt gcgatggat aatttgaaa aagaagggt 
      tacgtcctg tgggaccgc aaaattccg cttacaaga 
      aaacacatt gatttggtg gcgtcagct tacgggaaa 
      ttgcacgcg atttcgatc gcaatgcgc gaccaagaa 
      ccacaaagg tttgagagt ttggctaaa aaaccgttt 
      gattttttc gtcaaattt atgaaaatg tccgatatg 
      atcacgaca atggagcgg aactttgat gaaataact 
      gatttgatg gcgggtgag ctcgaggat ggagttttg 
      aggaagtgg aaggattcc aagcggagg attcggccg 
      ttttgggag aatttgagg gaaattgtg actgggatg 
      aaggtgcta acacatggc gactgctgg aataataac 
      ttcatgcat taccacatc aacggagaa ccggcggat 
      tttgtgatg atcgactgg cagttgatt tacctaact 
      tcgccaatt tatgacttg tcgtatttt ctcttcgct 
      tgtatttcc gaggaggac attaacgat ttggataaa 
      attttacaa ctgtaccac agttcgtta cgagaccat 
      ttgaagaaa ttgggaagt aaccccgat ttgtaccct 
      tttgaggat ttggtaaat gactggaaa aagtacagc 
      ttttacgga gtaataatc acgaatata attcacaaa 
      tttgtgcaa agcgacaaa gacgaggtc cccgatttg 
      gtcgattcg gtcgaaagt ggcgaagat atggcggac 
      gttttcaat ttaccaatc aagaataca gaagactac 
      aaaaaacgg cggttgtat atcacgaaa tatgtagtc 
      gaacaagaa atgtcgact caactcgaa gctaacatc 
      aaacagtgg ctagcaaaa gccctaaaa tctcaaaac 
      ctgtccgac ttttccgta aaactcgtc ggaaattcg 
      ggcaaaggc gacggttac gtcggtgat ataactttc 
      gtgcaactc tcaacccct caccaaacc tacaatctg 
      gtggtaaaa tgcagcaaa cagtccaag gttttcact 
      cgaattttc ctccgcgag atatttttc tacgacact 
      ctattaccc actttcatc aaattccaa aaagagaga 
      aatttggcg gaaatcttc ggcagtgtg gccaaatgt 
      tacggaacc tacacgagt gaaaatatc cacgttata 
      atcctagaa gacttgaag aaactgggg tatgaattg 
      tggccagtg tccaagcca atgaaccgc gaacatatt 
      gaaatggta gtgcgagaa tatggcaag tttcatgca 
      acttcagtg gcgctgaaa attctgaag cctgaaaag 
      tttcaagag attttagct acgttgagt cgcactgtg 
      agcgacatg ttccggacg ccggaaatg caaagtatc 
      gtcaggaaa tctttgaaa aaagcgatc gagttgatt 
      gaggaggaa gtggcggag gaaatcgtc acaaaatta 
      aaacacttg gtggaaaaa atacaaaac acgactcta 
      gaatttgag gaaaattca cgcctgaac gtggtgtgc 
      catggtggt tgttggaat aacaattac atgtataaa 
      tatgatggt agtactaaa aatgttccg tcaaaactt 
      gtcataatc gactggcaa atagcaact tgtggaaca 
      cccattgcg gacttgtct tatttcctc tttagttac 
      atttcggcc aaagatatc gaacatttg gaagatctt 
      ttgcttgtt taccaccag tcgttttca aatcacttg 
      gaaaagttc ggtttaatt cctgaaaat ttgtacccg 
      cgtgagcag tttttaaac gattggcaa gcctgtagc 
      aaatatgga cttgtattg gcgattaga agcgtacga 
      acttctttc atcgaaaaa gatgagatc gtcgatttg 
      gaagaggcg ctccaacag ggagtcgaa ctggccgat 
      tattttacc agagacata agaaacatg aagggatat 
      aaaagcagg attacgtac cttgtaaac agtaaataa
    mRNA sequence
    • >TC009653 RA
      agttttgcg caaaaatgt cccacacta gcaacgaga 
      tcgaggtaa aatcgtggt tggccaaaa ttttgaaaa 
      acgctgata acttctcgg tgaaaattg agggaaact 
      cggaaaagg gtgatggct accttggag acatccttt 
      tcgtccaag tgactgaaa acaccgcca aaaaacaca 
      atctcgtgc taaaatgtg caaagaaga gtaaactcc 
      tgcgcgaga aatcgccag ttcaggagg cttatttga 
      acgaaattt tgttctacg aaactgttt accccgatt 
      ttgtgcgtt ttcaaaggg agagaggtg tggagaacg 
      ggttcgata acgtggtaa agtgttacg ggacgttga 
      tcagcggcg atatggaaa taattgcga tggataatt 
      tgaaaaaga agggttacg tcctgtggg accgcaaaa 
      ttccgctta caagaaaac acattgatt tggtggcgt 
      cagcttacg ggaaattgc acgcgattt cgatcgcaa 
      tgcgcgacc aagaaccac aaaggtttg agagtttgg 
      ctaaaaaac cgtttgatt ttttcgtca aatttatga 
      aaatgtccg atatgatca cgacaatgg agcggaact 
      ttgatgaaa taactgatt tgatggcgg gtgagctcg 
      aggatggag ttttgagga agtggaagg attccaagc 
      ggaggattc ggccgtttt gggagaatt tgagggaaa 
      ttgtgactg ggatgaagg tgctaacac atggcgact 
      gctggaata ataacttca tgcattacc acatcaacg 
      gagaaccgg cggattttg tgatgatcg actggcagt 
      tgatttacc taacttcgc caatttatg acttgtcgt 
      attttctct tcgcttgta tttccgagg aggacatta 
      acgatttgg ataaaattt tacaactgt accacagtt 
      cgttacgag accatttga agaaattgg gaagtaacc 
      ccgatttgt acccttttg aggatttgg taaatgact 
      ggaaaaagt acagctttt acggagtaa taatcacga 
      atataattc acaaatttg tgcaaagcg acaaagacg 
      aggtccccg atttggtcg attcggtcg aaagtggcg 
      aagatatgg cggacgttt tcaatttac caatcaaga 
      atacagaag actacaaaa aacggcggt tgtatatca 
      cgaaatatg tagtcgaac aagaaatgt cgactcaac 
      tcgaagcta acatcaaac agtggctag caaaagccc 
      taaaatctc aaaacctgt ccgactttt ccgtaaaac 
      tcgtcggaa attcgggca aaggcgacg gttacgtcg 
      gtgatataa ctttcgtgc aactctcaa cccctcacc 
      aaacctaca atctggtgg taaaatgca gcaaacagt 
      ccaaggttt tcactcgaa ttttcctcc gcgagatat 
      ttttctacg acactctat tacccactt tcatcaaat 
      tccaaaaag agagaaatt tggcggaaa tcttcggca 
      gtgtggcca aatgttacg gaacctaca cgagtgaaa 
      atatccacg ttataatcc tagaagact tgaagaaac 
      tggggtatg aattgtggc cagtgtcca agccaatga 
      accgcgaac atattgaaa tggtagtgc gagaatatg 
      gcaagtttc atgcaactt cagtggcgc tgaaaattc 
      tgaagcctg aaaagtttc aagagattt tagctacgt 
      tgagtcgca ctgtgagcg acatgttcc ggacgccgg 
      aaatgcaaa gtatcgtca ggaaatctt tgaaaaaag 
      cgatcgagt tgattgagg aggaagtgg cggaggaaa 
      tcgtcacaa aattaaaac acttggtgg aaaaaatac 
      aaaacacga ctctagaat ttgaggaaa attcacgcc 
      tgaacgtgg tgtgccatg gtggttgtt ggaataaca 
      attacatgt ataaatatg atggtagta ctaaaaatg 
      ttccgtcaa aacttgtca taatcgact ggcaaatag 
      caacttgtg gaacaccca ttgcggact tgtcttatt 
      tcctcttta gttacattt cggccaaag atatcgaac 
      atttggaag atcttttgc ttgtttacc accagtcgt 
      tttcaaatc acttggaaa agttcggtt taattcctg 
      aaaatttgt acccgcgtg agcagtttt taaacgatt 
      ggcaagcct gtagcaaat atggacttg tattggcga 
      ttagaagcg tacgaactt ctttcatcg aaaaagatg 
      agatcgtcg atttggaag aggcgctcc aacagggag 
      tcgaactgg ccgattatt ttaccagag acataagaa 
      acatgaagg gatataaaa gcaggatta cgtaccttg 
      taaacagta aataaaaca aaatagctg gggattttt 
      tatttgtta caaaagtca cacagttaa tccaaattg 
      cgtaggtag tgcaaaaaa ttgctcttg acccttcga 
      taaaactcg tccttgtcc gaaaatttc atcgaaaaa 
      gatgagatc gtcgatttg gaagaggcg ctccaacag 
      ggagtcgaa ctggccgat tattttacc agagacata 
      agaaacatg aagggatat aaaagcagg attacgtac 
      cttgtaaag tacgcccac aaacatgga ttattttaa 
      ataacacta atttacaaa aaaataaaa ttttaccaa 
      gaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY