Status
Please wait ...
Gene information
General information
TC009653 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3615178..3620675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009653
    iBeetle screen iB_04672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009653 RA
      MSHTSNEIE VKSWLAKIL KNADNFSVK IEGNSEKGD 
GYLGDILFV QVTENTAKK HNLVLKCAK KSKLLREKS 
PVQEAYLNE ILFYETVYP DFVRFQRER GVENGFDNV 
VKCYGTLIS GDMEIIAMD NLKKKGYVL WDRKIPLTR 
KHIDLVASA YGKLHAISI AMRDQEPQR FESLAKKPF 
DFFVKFMKM SDMITTMER NFDEITDLM AGELEDGVL 
RKWKDSKRR IRPFWENLR EIVTGMKVL THGDCWNNN 
FMHYHINGE PADFVMIDW QLIYLTSPI YDLSYFLFA 
CISEEDIND LDKILQLYH SSLRDHLKK LGSNPDLYP 
FEDLVNDWK KYSFYGVII TNIIHKFVQ SDKDEVPDL 
VDSVESGED MADVFNLPI KNTEDYKKR RLYITKYVV 
EQEMSTQLE ANIKQWLAK ALKSQNLSD FSVKLVGNS 
GKGDGYVGD ITFVQLSTP HQTYNLVVK CSKQSKVFT 
RIFLREIFF YDTLLPTFI KFQKERNLA EIFGSVAKC 
YGTYTSENI HVIILEDLK KLGYELWPV SKPMNREHI 
EMVVREYGK FHATSVALK ILKPEKFQE ILATLSRTV 
SDMFRTPEM QSIVRKSLK KAIELIEEE VAEEIVTKL 
KHLVEKIQN TTLEFEENS RLNVVCHGG CWNNNYMYK 
YDGSTKNVP SKLVIIDWQ IATCGTPIA DLSYFLFSY 
ISAKDIEHL EDLLLVYHQ SFSNHLEKF GLIPENLYP 
REQFLNDWQ ACSKYGLVL AIRSVRTSF IEKDEIVDL 
EEALQQGVE LADYFTRDI RNMKGYKSR ITYLVNSK
    CDS sequence
    • >TC009653 RA
      atgtcccac actagcaac gagatcgag gtaaaatcg 
tggttggcc aaaattttg aaaaacgct gataacttc 
tcggtgaaa attgaggga aactcggaa aagggtgat 
ggctacctt ggagacatc cttttcgtc caagtgact 
gaaaacacc gccaaaaaa cacaatctc gtgctaaaa 
tgtgcaaag aagagtaaa ctcctgcgc gagaaatcg 
ccagttcag gaggcttat ttgaacgaa attttgttc 
tacgaaact gtttacccc gattttgtg cgttttcaa 
agggagaga ggtgtggag aacgggttc gataacgtg 
gtaaagtgt tacgggacg ttgatcagc ggcgatatg 
gaaataatt gcgatggat aatttgaaa aagaagggt 
tacgtcctg tgggaccgc aaaattccg cttacaaga 
aaacacatt gatttggtg gcgtcagct tacgggaaa 
ttgcacgcg atttcgatc gcaatgcgc gaccaagaa 
ccacaaagg tttgagagt ttggctaaa aaaccgttt 
gattttttc gtcaaattt atgaaaatg tccgatatg 
atcacgaca atggagcgg aactttgat gaaataact 
gatttgatg gcgggtgag ctcgaggat ggagttttg 
aggaagtgg aaggattcc aagcggagg attcggccg 
ttttgggag aatttgagg gaaattgtg actgggatg 
aaggtgcta acacatggc gactgctgg aataataac 
ttcatgcat taccacatc aacggagaa ccggcggat 
tttgtgatg atcgactgg cagttgatt tacctaact 
tcgccaatt tatgacttg tcgtatttt ctcttcgct 
tgtatttcc gaggaggac attaacgat ttggataaa 
attttacaa ctgtaccac agttcgtta cgagaccat 
ttgaagaaa ttgggaagt aaccccgat ttgtaccct 
tttgaggat ttggtaaat gactggaaa aagtacagc 
ttttacgga gtaataatc acgaatata attcacaaa 
tttgtgcaa agcgacaaa gacgaggtc cccgatttg 
gtcgattcg gtcgaaagt ggcgaagat atggcggac 
gttttcaat ttaccaatc aagaataca gaagactac 
aaaaaacgg cggttgtat atcacgaaa tatgtagtc 
gaacaagaa atgtcgact caactcgaa gctaacatc 
aaacagtgg ctagcaaaa gccctaaaa tctcaaaac 
ctgtccgac ttttccgta aaactcgtc ggaaattcg 
ggcaaaggc gacggttac gtcggtgat ataactttc 
gtgcaactc tcaacccct caccaaacc tacaatctg 
gtggtaaaa tgcagcaaa cagtccaag gttttcact 
cgaattttc ctccgcgag atatttttc tacgacact 
ctattaccc actttcatc aaattccaa aaagagaga 
aatttggcg gaaatcttc ggcagtgtg gccaaatgt 
tacggaacc tacacgagt gaaaatatc cacgttata 
atcctagaa gacttgaag aaactgggg tatgaattg 
tggccagtg tccaagcca atgaaccgc gaacatatt 
gaaatggta gtgcgagaa tatggcaag tttcatgca 
acttcagtg gcgctgaaa attctgaag cctgaaaag 
tttcaagag attttagct acgttgagt cgcactgtg 
agcgacatg ttccggacg ccggaaatg caaagtatc 
gtcaggaaa tctttgaaa aaagcgatc gagttgatt 
gaggaggaa gtggcggag gaaatcgtc acaaaatta 
aaacacttg gtggaaaaa atacaaaac acgactcta 
gaatttgag gaaaattca cgcctgaac gtggtgtgc 
catggtggt tgttggaat aacaattac atgtataaa 
tatgatggt agtactaaa aatgttccg tcaaaactt 
gtcataatc gactggcaa atagcaact tgtggaaca 
cccattgcg gacttgtct tatttcctc tttagttac 
atttcggcc aaagatatc gaacatttg gaagatctt 
ttgcttgtt taccaccag tcgttttca aatcacttg 
gaaaagttc ggtttaatt cctgaaaat ttgtacccg 
cgtgagcag tttttaaac gattggcaa gcctgtagc 
aaatatgga cttgtattg gcgattaga agcgtacga 
acttctttc atcgaaaaa gatgagatc gtcgatttg 
gaagaggcg ctccaacag ggagtcgaa ctggccgat 
tattttacc agagacata agaaacatg aagggatat 
aaaagcagg attacgtac cttgtaaac agtaaataa
    mRNA sequence
    • >TC009653 RA
      agttttgcg caaaaatgt cccacacta gcaacgaga 
tcgaggtaa aatcgtggt tggccaaaa ttttgaaaa 
acgctgata acttctcgg tgaaaattg agggaaact 
cggaaaagg gtgatggct accttggag acatccttt 
tcgtccaag tgactgaaa acaccgcca aaaaacaca 
atctcgtgc taaaatgtg caaagaaga gtaaactcc 
tgcgcgaga aatcgccag ttcaggagg cttatttga 
acgaaattt tgttctacg aaactgttt accccgatt 
ttgtgcgtt ttcaaaggg agagaggtg tggagaacg 
ggttcgata acgtggtaa agtgttacg ggacgttga 
tcagcggcg atatggaaa taattgcga tggataatt 
tgaaaaaga agggttacg tcctgtggg accgcaaaa 
ttccgctta caagaaaac acattgatt tggtggcgt 
cagcttacg ggaaattgc acgcgattt cgatcgcaa 
tgcgcgacc aagaaccac aaaggtttg agagtttgg 
ctaaaaaac cgtttgatt ttttcgtca aatttatga 
aaatgtccg atatgatca cgacaatgg agcggaact 
ttgatgaaa taactgatt tgatggcgg gtgagctcg 
aggatggag ttttgagga agtggaagg attccaagc 
ggaggattc ggccgtttt gggagaatt tgagggaaa 
ttgtgactg ggatgaagg tgctaacac atggcgact 
gctggaata ataacttca tgcattacc acatcaacg 
gagaaccgg cggattttg tgatgatcg actggcagt 
tgatttacc taacttcgc caatttatg acttgtcgt 
attttctct tcgcttgta tttccgagg aggacatta 
acgatttgg ataaaattt tacaactgt accacagtt 
cgttacgag accatttga agaaattgg gaagtaacc 
ccgatttgt acccttttg aggatttgg taaatgact 
ggaaaaagt acagctttt acggagtaa taatcacga 
atataattc acaaatttg tgcaaagcg acaaagacg 
aggtccccg atttggtcg attcggtcg aaagtggcg 
aagatatgg cggacgttt tcaatttac caatcaaga 
atacagaag actacaaaa aacggcggt tgtatatca 
cgaaatatg tagtcgaac aagaaatgt cgactcaac 
tcgaagcta acatcaaac agtggctag caaaagccc 
taaaatctc aaaacctgt ccgactttt ccgtaaaac 
tcgtcggaa attcgggca aaggcgacg gttacgtcg 
gtgatataa ctttcgtgc aactctcaa cccctcacc 
aaacctaca atctggtgg taaaatgca gcaaacagt 
ccaaggttt tcactcgaa ttttcctcc gcgagatat 
ttttctacg acactctat tacccactt tcatcaaat 
tccaaaaag agagaaatt tggcggaaa tcttcggca 
gtgtggcca aatgttacg gaacctaca cgagtgaaa 
atatccacg ttataatcc tagaagact tgaagaaac 
tggggtatg aattgtggc cagtgtcca agccaatga 
accgcgaac atattgaaa tggtagtgc gagaatatg 
gcaagtttc atgcaactt cagtggcgc tgaaaattc 
tgaagcctg aaaagtttc aagagattt tagctacgt 
tgagtcgca ctgtgagcg acatgttcc ggacgccgg 
aaatgcaaa gtatcgtca ggaaatctt tgaaaaaag 
cgatcgagt tgattgagg aggaagtgg cggaggaaa 
tcgtcacaa aattaaaac acttggtgg aaaaaatac 
aaaacacga ctctagaat ttgaggaaa attcacgcc 
tgaacgtgg tgtgccatg gtggttgtt ggaataaca 
attacatgt ataaatatg atggtagta ctaaaaatg 
ttccgtcaa aacttgtca taatcgact ggcaaatag 
caacttgtg gaacaccca ttgcggact tgtcttatt 
tcctcttta gttacattt cggccaaag atatcgaac 
atttggaag atcttttgc ttgtttacc accagtcgt 
tttcaaatc acttggaaa agttcggtt taattcctg 
aaaatttgt acccgcgtg agcagtttt taaacgatt 
ggcaagcct gtagcaaat atggacttg tattggcga 
ttagaagcg tacgaactt ctttcatcg aaaaagatg 
agatcgtcg atttggaag aggcgctcc aacagggag 
tcgaactgg ccgattatt ttaccagag acataagaa 
acatgaagg gatataaaa gcaggatta cgtaccttg 
taaacagta aataaaaca aaatagctg gggattttt 
tatttgtta caaaagtca cacagttaa tccaaattg 
cgtaggtag tgcaaaaaa ttgctcttg acccttcga 
taaaactcg tccttgtcc gaaaatttc atcgaaaaa 
gatgagatc gtcgatttg gaagaggcg ctccaacag 
ggagtcgaa ctggccgat tattttacc agagacata 
agaaacatg aagggatat aaaagcagg attacgtac 
cttgtaaag tacgcccac aaacatgga ttattttaa 
ataacacta atttacaaa aaaataaaa ttttaccaa 
gaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY