| General information | |||
| TC009653 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3615178..3620675 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009653 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04672 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC009653 RA
MSHTSNEIE VKSWLAKIL KNADNFSVK IEGNSEKGD GYLGDILFV QVTENTAKK HNLVLKCAK KSKLLREKS PVQEAYLNE ILFYETVYP DFVRFQRER GVENGFDNV VKCYGTLIS GDMEIIAMD NLKKKGYVL WDRKIPLTR KHIDLVASA YGKLHAISI AMRDQEPQR FESLAKKPF DFFVKFMKM SDMITTMER NFDEITDLM AGELEDGVL RKWKDSKRR IRPFWENLR EIVTGMKVL THGDCWNNN FMHYHINGE PADFVMIDW QLIYLTSPI YDLSYFLFA CISEEDIND LDKILQLYH SSLRDHLKK LGSNPDLYP FEDLVNDWK KYSFYGVII TNIIHKFVQ SDKDEVPDL VDSVESGED MADVFNLPI KNTEDYKKR RLYITKYVV EQEMSTQLE ANIKQWLAK ALKSQNLSD FSVKLVGNS GKGDGYVGD ITFVQLSTP HQTYNLVVK CSKQSKVFT RIFLREIFF YDTLLPTFI KFQKERNLA EIFGSVAKC YGTYTSENI HVIILEDLK KLGYELWPV SKPMNREHI EMVVREYGK FHATSVALK ILKPEKFQE ILATLSRTV SDMFRTPEM QSIVRKSLK KAIELIEEE VAEEIVTKL KHLVEKIQN TTLEFEENS RLNVVCHGG CWNNNYMYK YDGSTKNVP SKLVIIDWQ IATCGTPIA DLSYFLFSY ISAKDIEHL EDLLLVYHQ SFSNHLEKF GLIPENLYP REQFLNDWQ ACSKYGLVL AIRSVRTSF IEKDEIVDL EEALQQGVE LADYFTRDI RNMKGYKSR ITYLVNSK
- >TC009653 RA
atgtcccac actagcaac gagatcgag gtaaaatcg tggttggcc aaaattttg aaaaacgct gataacttc tcggtgaaa attgaggga aactcggaa aagggtgat ggctacctt ggagacatc cttttcgtc caagtgact gaaaacacc gccaaaaaa cacaatctc gtgctaaaa tgtgcaaag aagagtaaa ctcctgcgc gagaaatcg ccagttcag gaggcttat ttgaacgaa attttgttc tacgaaact gtttacccc gattttgtg cgttttcaa agggagaga ggtgtggag aacgggttc gataacgtg gtaaagtgt tacgggacg ttgatcagc ggcgatatg gaaataatt gcgatggat aatttgaaa aagaagggt tacgtcctg tgggaccgc aaaattccg cttacaaga aaacacatt gatttggtg gcgtcagct tacgggaaa ttgcacgcg atttcgatc gcaatgcgc gaccaagaa ccacaaagg tttgagagt ttggctaaa aaaccgttt gattttttc gtcaaattt atgaaaatg tccgatatg atcacgaca atggagcgg aactttgat gaaataact gatttgatg gcgggtgag ctcgaggat ggagttttg aggaagtgg aaggattcc aagcggagg attcggccg ttttgggag aatttgagg gaaattgtg actgggatg aaggtgcta acacatggc gactgctgg aataataac ttcatgcat taccacatc aacggagaa ccggcggat tttgtgatg atcgactgg cagttgatt tacctaact tcgccaatt tatgacttg tcgtatttt ctcttcgct tgtatttcc gaggaggac attaacgat ttggataaa attttacaa ctgtaccac agttcgtta cgagaccat ttgaagaaa ttgggaagt aaccccgat ttgtaccct tttgaggat ttggtaaat gactggaaa aagtacagc ttttacgga gtaataatc acgaatata attcacaaa tttgtgcaa agcgacaaa gacgaggtc cccgatttg gtcgattcg gtcgaaagt ggcgaagat atggcggac gttttcaat ttaccaatc aagaataca gaagactac aaaaaacgg cggttgtat atcacgaaa tatgtagtc gaacaagaa atgtcgact caactcgaa gctaacatc aaacagtgg ctagcaaaa gccctaaaa tctcaaaac ctgtccgac ttttccgta aaactcgtc ggaaattcg ggcaaaggc gacggttac gtcggtgat ataactttc gtgcaactc tcaacccct caccaaacc tacaatctg gtggtaaaa tgcagcaaa cagtccaag gttttcact cgaattttc ctccgcgag atatttttc tacgacact ctattaccc actttcatc aaattccaa aaagagaga aatttggcg gaaatcttc ggcagtgtg gccaaatgt tacggaacc tacacgagt gaaaatatc cacgttata atcctagaa gacttgaag aaactgggg tatgaattg tggccagtg tccaagcca atgaaccgc gaacatatt gaaatggta gtgcgagaa tatggcaag tttcatgca acttcagtg gcgctgaaa attctgaag cctgaaaag tttcaagag attttagct acgttgagt cgcactgtg agcgacatg ttccggacg ccggaaatg caaagtatc gtcaggaaa tctttgaaa aaagcgatc gagttgatt gaggaggaa gtggcggag gaaatcgtc acaaaatta aaacacttg gtggaaaaa atacaaaac acgactcta gaatttgag gaaaattca cgcctgaac gtggtgtgc catggtggt tgttggaat aacaattac atgtataaa tatgatggt agtactaaa aatgttccg tcaaaactt gtcataatc gactggcaa atagcaact tgtggaaca cccattgcg gacttgtct tatttcctc tttagttac atttcggcc aaagatatc gaacatttg gaagatctt ttgcttgtt taccaccag tcgttttca aatcacttg gaaaagttc ggtttaatt cctgaaaat ttgtacccg cgtgagcag tttttaaac gattggcaa gcctgtagc aaatatgga cttgtattg gcgattaga agcgtacga acttctttc atcgaaaaa gatgagatc gtcgatttg gaagaggcg ctccaacag ggagtcgaa ctggccgat tattttacc agagacata agaaacatg aagggatat aaaagcagg attacgtac cttgtaaac agtaaataa
- >TC009653 RA
agttttgcg caaaaatgt cccacacta gcaacgaga tcgaggtaa aatcgtggt tggccaaaa ttttgaaaa acgctgata acttctcgg tgaaaattg agggaaact cggaaaagg gtgatggct accttggag acatccttt tcgtccaag tgactgaaa acaccgcca aaaaacaca atctcgtgc taaaatgtg caaagaaga gtaaactcc tgcgcgaga aatcgccag ttcaggagg cttatttga acgaaattt tgttctacg aaactgttt accccgatt ttgtgcgtt ttcaaaggg agagaggtg tggagaacg ggttcgata acgtggtaa agtgttacg ggacgttga tcagcggcg atatggaaa taattgcga tggataatt tgaaaaaga agggttacg tcctgtggg accgcaaaa ttccgctta caagaaaac acattgatt tggtggcgt cagcttacg ggaaattgc acgcgattt cgatcgcaa tgcgcgacc aagaaccac aaaggtttg agagtttgg ctaaaaaac cgtttgatt ttttcgtca aatttatga aaatgtccg atatgatca cgacaatgg agcggaact ttgatgaaa taactgatt tgatggcgg gtgagctcg aggatggag ttttgagga agtggaagg attccaagc ggaggattc ggccgtttt gggagaatt tgagggaaa ttgtgactg ggatgaagg tgctaacac atggcgact gctggaata ataacttca tgcattacc acatcaacg gagaaccgg cggattttg tgatgatcg actggcagt tgatttacc taacttcgc caatttatg acttgtcgt attttctct tcgcttgta tttccgagg aggacatta acgatttgg ataaaattt tacaactgt accacagtt cgttacgag accatttga agaaattgg gaagtaacc ccgatttgt acccttttg aggatttgg taaatgact ggaaaaagt acagctttt acggagtaa taatcacga atataattc acaaatttg tgcaaagcg acaaagacg aggtccccg atttggtcg attcggtcg aaagtggcg aagatatgg cggacgttt tcaatttac caatcaaga atacagaag actacaaaa aacggcggt tgtatatca cgaaatatg tagtcgaac aagaaatgt cgactcaac tcgaagcta acatcaaac agtggctag caaaagccc taaaatctc aaaacctgt ccgactttt ccgtaaaac tcgtcggaa attcgggca aaggcgacg gttacgtcg gtgatataa ctttcgtgc aactctcaa cccctcacc aaacctaca atctggtgg taaaatgca gcaaacagt ccaaggttt tcactcgaa ttttcctcc gcgagatat ttttctacg acactctat tacccactt tcatcaaat tccaaaaag agagaaatt tggcggaaa tcttcggca gtgtggcca aatgttacg gaacctaca cgagtgaaa atatccacg ttataatcc tagaagact tgaagaaac tggggtatg aattgtggc cagtgtcca agccaatga accgcgaac atattgaaa tggtagtgc gagaatatg gcaagtttc atgcaactt cagtggcgc tgaaaattc tgaagcctg aaaagtttc aagagattt tagctacgt tgagtcgca ctgtgagcg acatgttcc ggacgccgg aaatgcaaa gtatcgtca ggaaatctt tgaaaaaag cgatcgagt tgattgagg aggaagtgg cggaggaaa tcgtcacaa aattaaaac acttggtgg aaaaaatac aaaacacga ctctagaat ttgaggaaa attcacgcc tgaacgtgg tgtgccatg gtggttgtt ggaataaca attacatgt ataaatatg atggtagta ctaaaaatg ttccgtcaa aacttgtca taatcgact ggcaaatag caacttgtg gaacaccca ttgcggact tgtcttatt tcctcttta gttacattt cggccaaag atatcgaac atttggaag atcttttgc ttgtttacc accagtcgt tttcaaatc acttggaaa agttcggtt taattcctg aaaatttgt acccgcgtg agcagtttt taaacgatt ggcaagcct gtagcaaat atggacttg tattggcga ttagaagcg tacgaactt ctttcatcg aaaaagatg agatcgtcg atttggaag aggcgctcc aacagggag tcgaactgg ccgattatt ttaccagag acataagaa acatgaagg gatataaaa gcaggatta cgtaccttg taaacagta aataaaaca aaatagctg gggattttt tatttgtta caaaagtca cacagttaa tccaaattg cgtaggtag tgcaaaaaa ttgctcttg acccttcga taaaactcg tccttgtcc gaaaatttc atcgaaaaa gatgagatc gtcgatttg gaagaggcg ctccaacag ggagtcgaa ctggccgat tattttacc agagacata agaaacatg aagggatat aaaagcagg attacgtac cttgtaaag tacgcccac aaacatgga ttattttaa ataacacta atttacaaa aaaataaaa ttttaccaa gaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
