Status
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Gene information
General information
TC033594
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009634,TC009635
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3466625..3470934
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033594
    iBeetle screen iB_04669

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033594 RA
      MLKLITVTI FVCLLSATS CLDNGLALT PPMGWMHWE 
      RFRCIIDCD NYPTDCISE NLFMDMADK MASDGYLDA 
      GYEYIIVDD CWLAMERDS EGRLQADPD RFPSGMKFL 
      ADYVHAKGL KFGIYEDYG THTCGGYPG SIDHLELDA 
      QTFADWGVD YLKLDGCYA ELDGMEDGY AEMEAYLNK 
      TQRPIVFSC SFPAYKGLD ANYSAAVEH CNLWRNYGD 
      IQDSWDSVV KITNWFSYY QDKLLPYAG PGHWNDPDM 
      LIIGNYGLS YDQSVAQMT IWSVMAAPL IMSVDLRTI 
      KPEFRDILL NKDAIGINQ DVLGKPGVV KYNKNKIKI 
      WVKELVDGS YAIGIASFR VDGKPYQIE IVLKDYGLD 
      NEGYLINNV FEDVEPISV SVTEPIKVL INPTGGVLW 
      KLIPATKVV VEVTFRKFG NKCSNYKMF LFFVLSLCT 
      LSAHALDNG LALTPPMGW LHWERFRCE TDCRQFPFD 
      CISENLFKA MADKMASDG YLDAGYEYV IMDDCWLAM 
      DRDSEGRLQ ADPDRFPSG IKALADYVH AKGLKFGIY 
      EDYGTKTCA GYPGSLDHL EIDAKTFAE WGVDYLKMD 
      GCNVTPDEA MEAGHLEMA RYLNETGRE IVFSCEFPL 
      YRGDKANYS VAIEACNLW RNYNDIEDS WVSVTNIVN 
      HYKKNQDKY VAVAGPGHW NDPDMLIIG NFGLSLDQS 
      KAQMTIWAI WAAPLIMSV DLRTIKPEF KEILLNKHA 
      IKINQDPLG IQGHLQTTI NDVDVWLKP ISPNVGGEF 
      SYAVGFVSN RDDGYPYRI EFTLRDFGL NNTAGYVLT 
      DAFVSDETT VVKNTDKLE VFVNPSGAV LWHLVANEA
    CDS sequence
    • >TC033594 RA
      atgttaaaa ctaatcacc gtaacgatt tttgtgtgc 
      ctcttgtct gcaacaagt tgtcttgac aatgggcta 
      gcccttacc cctccaatg gggtggatg cactgggag 
      cgcttccgg tgcatcatc gactgtgat aactacccc 
      actgactgt atcagtgag aacctgttc atggacatg 
      gccgacaaa atggcctct gacggttat ctggacgct 
      gggtacgag tacataatc gtggacgat tgctggttg 
      gccatggaa agggacagc gaagggcgc ctccaagcc 
      gaccctgac cgatttccc tccggaatg aaattccta 
      gccgactac gtccacgcc aaagggctc aaattcggg 
      atttatgag gactacggc acccacact tgtggggga 
      taccccgga agtatcgac catttggaa ctggacgcc 
      caaacgttc gctgattgg ggcgttgat tatttgaaa 
      ctggatggg tgttacgca gaattggac ggtatggag 
      gatggatat gctgaaatg gaggcgtat ctcaacaaa 
      acccaacgt cccattgtc ttttcgtgc agtttcccg 
      gcttataaa ggcctcgat gcgaattat agcgcggct 
      gttgagcac tgcaatctg tggaggaac tatggggat 
      attcaggac tcttgggac agtgttgtt aaaatcacc 
      aattggttc tcatattat caagacaaa ttgttgcca 
      tatgcgggc cctggacat tggaacgac ccagatatg 
      ttaattatt ggcaattac gggttgagt tatgaccaa 
      agcgttgcc caaatgaca atctggagt gtgatggca 
      gcccccttg atcatgtcg gttgatttg aggacgatc 
      aagcctgaa tttagggat attttacta aacaaagac 
      gcgattgga attaatcag gacgtttta gggaaacct 
      ggagttgta aaatacaat aaaaataaa attaaaatt 
      tgggttaaa gagcttgtt gacggttcg tatgccatc 
      ggtatcgca agttttcga gttgatggc aagccttat 
      cagattgaa attgttttg aaagattat ggtttggat 
      aatgagggc tacttgata aataatgtt tttgaggat 
      gttgagccg atttcggtt tcggttacc gagccgatc 
      aaagttttg attaaccca acgggtggt gtcctgtgg 
      aaacttatc ccagcgaca aaagttgta gttgaggtg 
      actttccga aagtttggt aataagtgt tcaaactac 
      aaaatgttt ctcttcttc gtcctctcc ctatgcacc 
      ctttccgcc cacgccctt gacaacggt ttggcccta 
      accccgccc atgggttgg ctgcactgg gagcgtttt 
      cggtgcgaa accgactgc cgccaattc cccttcgac 
      tgtattagt gagaattta tttaaagca atggccgac 
      aaaatggcc tctgatggt tatttggac gccggctac 
      gaatacgtt attatggat gattgttgg ttggctatg 
      gacagggac agtgaaggg cgtctccaa gccgaccct 
      gaccgattt ccctcggga atcaaggct ttggccgac 
      tatgtccat gccaaaggg ctcaaattc gggatttat 
      gaggattac gggactaag acttgtgcc ggatatccg 
      ggaagtctg gatcatttg gaaattgat gcaaagact 
      tttgcggaa tggggagtg gattatttg aaaatggac 
      ggatgtaac gtcactccg gatgaagca atggaggct 
      gggcattta gaaatggcg cgatatttg aatgaaaca 
      gggcgcgag attgtgttc tcatgcgag tttccctta 
      tatcgtggc gataaagcc aattacagc gtggcaatt 
      gaggcttgc aacctttgg cggaactat aacgatatt 
      gaggactcc tgggtcagt gttaccaac attgtcaac 
      cattataag aaaaatcag gataagtat gtggcggtt 
      gcagggccg gggcattgg aacgaccca gatatgttg 
      attatcggc aattttggg ctaagtctg gaccagagc 
      aaggcccaa atgaccata tgggccata tgggcggcc 
      cctctgatc atgtcggtg gacctgagg accatcaag 
      cccgaattt aaggaaatt ctcctcaac aaacacgcg 
      attaagatc aatcaagac cctttgggc atccaagga 
      cacttgcaa acaacgata aatgatgtt gatgtttgg 
      ctgaaacca atttcgcca aatgttggt ggtgaattt 
      tcatacgct gtgggattt gtcagtaat agggatgac 
      ggatatcct tatagaatt gaattcacc cttagggat 
      tttgggctg aataatacg gcaggatat gtgcttacg 
      gatgcgttc gtttctgac gaaacaact gtagtaaaa 
      aatacggac aagctcgaa gtttttgtt aatccctca 
      ggagctgtt ctctggcat ttggttgct aatgaagcg 
      tag
    mRNA sequence
    • >TC033594 RA
      aaaatttca cacaatagt ttgtggaag gtattgtac 
      ttgtgctcg ttaaaatgt taaaactaa tcaccgtaa 
      cgatttttg tgtgcctct tgtctgcaa caagttgtc 
      ttgacaatg ggctagccc ttacccctc caatggggt 
      ggatgcact gggagcgct tccggtgca tcatcgact 
      gtgataact accccactg actgtatca gtgagaacc 
      tgttcatgg acatggccg acaaaatgg cctctgacg 
      gttatctgg acgctgggt acgagtaca taatcgtgg 
      acgattgct ggttggcca tggaaaggg acagcgaag 
      ggcgcctcc aagccgacc ctgaccgat ttccctccg 
      gaatgaaat tcctagccg actacgtcc acgccaaag 
      ggctcaaat tcgggattt atgaggact acggcaccc 
      acacttgtg ggggatacc ccggaagta tcgaccatt 
      tggaactgg acgcccaaa cgttcgctg attggggcg 
      ttgattatt tgaaactgg atgggtgtt acgcagaat 
      tggacggta tggaggatg gatatgctg aaatggagg 
      cgtatctca acaaaaccc aacgtccca ttgtctttt 
      cgtgcagtt tcccggctt ataaaggcc tcgatgcga 
      attatagcg cggctgttg agcactgca atctgtgga 
      ggaactatg gggatattc aggactctt gggacagtg 
      ttgttaaaa tcaccaatt ggttctcat attatcaag 
      acaaattgt tgccatatg cgggccctg gacattgga 
      acgacccag atatgttaa ttattggca attacgggt 
      tgagttatg accaaagcg ttgcccaaa tgacaatct 
      ggagtgtga tggcagccc ccttgatca tgtcggttg 
      atttgagga cgatcaagc ctgaattta gggatattt 
      tactaaaca aagacgcga ttggaatta atcaggacg 
      ttttaggga aacctggag ttgtaaaat acaataaaa 
      ataaaatta aaatttggg ttaaagagc ttgttgacg 
      gttcgtatg ccatcggta tcgcaagtt ttcgagttg 
      atggcaagc cttatcaga ttgaaattg ttttgaaag 
      attatggtt tggataatg agggctact tgataaata 
      atgtttttg aggatgttg agccgattt cggtttcgg 
      ttaccgagc cgatcaaag ttttgatta acccaacgg 
      gtggtgtcc tgtggaaac ttatcccag cgacaaaag 
      ttgtagttg aggtgactt tccgaaagt ttggtaata 
      agtgttcaa actacaaaa tgtttctct tcttcgtcc 
      tctccctat gcacccttt ccgcccacg cccttgaca 
      acggtttgg ccctaaccc cgcccatgg gttggctgc 
      actgggagc gttttcggt gcgaaaccg actgccgcc 
      aattcccct tcgactgta ttagtgaga atttattta 
      aagcaatgg ccgacaaaa tggcctctg atggttatt 
      tggacgccg gctacgaat acgttatta tggatgatt 
      gttggttgg ctatggaca gggacagtg aagggcgtc 
      tccaagccg accctgacc gatttccct cgggaatca 
      aggctttgg ccgactatg tccatgcca aagggctca 
      aattcggga tttatgagg attacggga ctaagactt 
      gtgccggat atccgggaa gtctggatc atttggaaa 
      ttgatgcaa agacttttg cggaatggg gagtggatt 
      atttgaaaa tggacggat gtaacgtca ctccggatg 
      aagcaatgg aggctgggc atttagaaa tggcgcgat 
      atttgaatg aaacagggc gcgagattg tgttctcat 
      gcgagtttc ccttatatc gtggcgata aagccaatt 
      acagcgtgg caattgagg cttgcaacc tttggcgga 
      actataacg atattgagg actcctggg tcagtgtta 
      ccaacattg tcaaccatt ataagaaaa atcaggata 
      agtatgtgg cggttgcag ggccggggc attggaacg 
      acccagata tgttgatta tcggcaatt ttgggctaa 
      gtctggacc agagcaagg cccaaatga ccatatggg 
      ccatatggg cggcccctc tgatcatgt cggtggacc 
      tgaggacca tcaagcccg aatttaagg aaattctcc 
      tcaacaaac acgcgatta agatcaatc aagaccctt 
      tgggcatcc aaggacact tgcaaacaa cgataaatg 
      atgttgatg tttggctga aaccaattt cgccaaatg 
      ttggtggtg aattttcat acgctgtgg gatttgtca 
      gtaataggg atgacggat atccttata gaattgaat 
      tcaccctta gggattttg ggctgaata atacggcag 
      gatatgtgc ttacggatg cgttcgttt ctgacgaaa 
      caactgtag taaaaaata cggacaagc tcgaagttt 
      ttgttaatc cctcaggag ctgttctct ggcatttgg 
      ttgctaatg aagcgtaga gagtacaat aacaaatta 
      tcaacttgt tagtgaaca actttggct tgttgatcg 
      atataataa ataaaagtt taaaaaact g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY