Status
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Gene information
General information
TC009616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3274023..3276218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009616
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
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      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04665

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009616 RA
        MEEYSPIES DLDVPTSPR QHFLSLDSG KDVDLIKTA 
        DENAIEKLL QALHDVDYT IKQKLEVVE NLDITVQIV 
        SFKIFEATH EFKKKIGLV KDERNRLLS SIENRSELS 
        LILNDLDRK ITELEKQLD EKIRTFNTE KIGLQEQAE 
        AIIFQLDED ISLMQQKFE ELKSELSFG KSNLYRVQI 
        EAMNQKYTP SVEKYEENL HYYERLQLT GWRVEQSEV 
        KLSELLEEQ GLKFTPSGR LLTRTGSLL TFQEAQDKG 
        YFKEVKVSL DKVFQFFKD RDKLRLPDR LPTPDADFS 
        EICSTVESK MSSQDVKYL KQYVGKPLT LALAEITAK 
        MPRDPIHYL GHYLFKYRY NQELEEVQK REIEELTQE 
        RKRLAQEKW KKFVEEEAR NAVIEMILR AEQLAYENE 
        LKRIEEEQM LAQEEEEGG EEENAQMAE LLEEARDKF 
        E
      CDS sequence
      • >TC009616 RA
        atggaagaa tattctccc atagaatca gatttagac 
        gtgccgacg tctccccgc cagcacttc ttgtcgctg 
        gactccggc aaagacgtg gatttgatc aaaacagcc 
        gacgaaaat gcaattgaa aaattgctg caagcgctc 
        cacgacgtt gactacacc atcaagcag aagctggag 
        gtggtggaa aacctggac atcacagtg caaatcgtc 
        agttttaaa atcttcgaa gccacgcat gaatttaag 
        aagaaaatc ggtctcgtg aaggatgag agaaaccgg 
        ctgttatca tcgattgaa aatcggtcc gagttgagc 
        ttgattttg aacgacttg gaccggaaa atcaccgaa 
        ctggagaag caactcgac gagaaaatc cgcactttt 
        aacacggag aaaattggg ttgcaggaa caagccgaa 
        gccattatt ttccaactc gacgaagac atttcttta 
        atgcagcaa aaattcgaa gagcttaaa agcgagctt 
        agttttggt aaatccaac ctttatcgt gtgcaaatc 
        gaagcaatg aaccaaaaa tacacaccg tctgtcgaa 
        aagtacgag gaaaatttg cactattac gagagattg 
        caactaaca gggtggcgg gtcgagcaa tcagaagtg 
        aaactctca gaacttctg gaagagcaa ggcttgaag 
        ttcactccc tctggacgg cttctgacc cggaccggc 
        agtctcctc acgtttcag gaagcccaa gacaagggt 
        tacttcaag gaagtgaag gtctccttg gacaaagtt 
        ttccaattc ttcaaagat cgcgacaag ttgcgactc 
        cccgaccgg ttacctaca ccagacgca gatttcagc 
        gaaatttgt agcactgtt gagagcaaa atgtcgtca 
        caagacgtc aaatacttg aaacagtac gtggggaag 
        cccctaacc ctggccttg gccgaaata accgccaaa 
        atgccaagg gaccctatt cactacttg ggccattac 
        ttgttcaag tatcggtac aaccaggag ctggaggag 
        gtgcaaaag agggaaatt gaggaactg acccaggaa 
        cggaagcga ctggcacag gaaaaatgg aaaaaattc 
        gtcgaagaa gaggcgaga aacgccgtc atcgaaatg 
        atccttcgc gcggaacaa ctagcgtac gaaaacgag 
        ttaaaacga atcgaggag gaacaaatg ttagctcag 
        gaggaggaa gagggtggt gaagaggaa aacgcgcaa 
        atggccgag cttttggaa gaagcgcgc gacaaattc 
        gagtga
      mRNA sequence
      • >TC009616 RA
        aattcaaat ttgtcaaaa aaatggaag aatattctc 
        ccatagaat cagatttag acgtgccga cgtctcccc 
        gccagcact tcttgtcgc tggactccg gcaaagacg 
        tggatttga tcaaaacag ccgacgaaa atgcaattg 
        aaaaattgc tgcaagcgc tccacgacg ttgactaca 
        ccatcaagc agaagctgg aggtggtgg aaaacctgg 
        acatcacag tgcaaatcg tcagtttta aaatcttcg 
        aagccacgc atgaattta agaagaaaa tcggtctcg 
        tgaaggatg agagaaacc ggctgttat catcgattg 
        aaaatcggt ccgagttga gcttgattt tgaacgact 
        tggaccgga aaatcaccg aactggaga agcaactcg 
        acgagaaaa tccgcactt ttaacacgg agaaaattg 
        ggttgcagg aacaagccg aagccatta ttttccaac 
        tcgacgaag acatttctt taatgcagc aaaaattcg 
        aagagctta aaagcgagc ttagttttg gtaaatcca 
        acctttatc gtgtgcaaa tcgaagcaa tgaaccaaa 
        aatacacac cgtctgtcg aaaagtacg aggaaaatt 
        tgcactatt acgagagat tgcaactaa cagggtggc 
        gggtcgagc aatcagaag tgaaactct cagaacttc 
        tggaagagc aaggcttga agttcactc cctctggac 
        ggcttctga cccggaccg gcagtctcc tcacgtttc 
        aggaagccc aagacaagg gttacttca aggaagtga 
        aggtctcct tggacaaag ttttccaat tcttcaaag 
        atcgcgaca agttgcgac tccccgacc ggttaccta 
        caccagacg cagatttca gcgaaattt gtagcactg 
        ttgagagca aaatgtcgt cacaagacg tcaaatact 
        tgaaacagt acgtgggga agcccctaa ccctggcct 
        tggccgaaa taaccgcca aaatgccaa gggacccta 
        ttcactact tgggccatt acttgttca agtatcggt 
        acaaccagg agctggagg aggtgcaaa agagggaaa 
        ttgaggaac tgacccagg aacggaagc gactggcac 
        aggaaaaat ggaaaaaat tcgtcgaag aagaggcga 
        gaaacgccg tcatcgaaa tgatccttc gcgcggaac 
        aactagcgt acgaaaacg agttaaaac gaatcgagg 
        aggaacaaa tgttagctc aggaggagg aagagggtg 
        gtgaagagg aaaacgcgc aaatggccg agcttttgg 
        aagaagcgc gcgacaaat tcgagtgaa acttaccaa 
        cactagtca cggcccatc ctttggatc acctgcgaa 
        acaaaataa taaattcag tgtcggcaa tgttgtttg 
        ttcctattt aagcggaga gtttgtaat tttgcatcc 
        gtttttgta cgcaaaaat ccgtgcctc aaaacaata 
        caagtacct cacctgtta aaacgcaat tcttgacga 
        atgcggcga gtttagttt tcgaaatgg accttggat 
        ggggacgca cacgtgggt cttcgaggg ggcgtccac 
        gtcgcattt tacaaaacg agttttgtg cgaattttc 
        ctcaggcgt tgggtttag taaaactcg attttctac 
        aaaagtcgc tcaatgttt gggctaaga cgtgattta 
        aacaatgcc aagctgtgc ctgttactg aactttgaa 
        atttaatta tacgaaaag aatccattc aggatgttt 
        ttgaataag ggttgctga gtcttgttt tagggcaaa 
        ctgttttaa cgaaacttc ataaaatta agaccattt 
        ccgttttca aagtcaaat tgacgaaac tagagattc 
        cgcaaaaac cggtccgaa atttcacta agtatgacg 
        ttatttttg ccatttttc gtgttaaca gctagaaac 
        gcaataatt ttttgttaa attg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY