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Gene information
General information
TC009612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3258326..3259744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009612
    iBeetle screen iB_04664

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009612 RA
      MLPCPGWYC GRQYLPNGS LSECGSCPR GFRRNDTTF 
      ICEPCTDSP TFYDWLYLG FMVLLVQIL HWFFIDMVS 
      MRRSFKKDV LILHLTALV EVVISGLLS VLFTHPFGE 
      FQVRNCRVR SLADWYTLL HNPAPDYEK RVHCTQEAV 
      YPLYTLVFI FYALTLIVM LLIRPWVCR KYLPRQSKM 
      SIYAAMYYI PILVVVHAL IGGLLYYAF PILVVILSV 
      ISCAYHFAL KINQSIPSL IITTVVDPR NVVILVGHW 
      CLHAYGLIS ITQLSDPTF HSLLLLLVP LPAFFYIFT 
      ARFTDPTIF T
    CDS sequence
    • >TC009612 RA
      atgcttccg tgtcctggc tggtactgc ggccgccag 
      tacctcccc aatggcagt ctcagcgag tgcgggtcg 
      tgcccccgg ggcttccgg cgcaacgac actactttt 
      atttgcgag ccgtgcacc gacagcccc acgttctac 
      gactggctt tatttgggg tttatggtg ctcctggtc 
      cagattctg cactggttt ttcatcgac atggtgtcg 
      atgcgacga agcttcaaa aaagacgtc ctaatttta 
      cacttgact gcgctggtg gaagtggtc atttcgggg 
      ctcttgtcg gtgcttttc acgcacccg ttcggcgag 
      ttccaggtg cggaactgc agagtgcgc agcttggcc 
      gattggtac actttgttg cataatccg gccccggat 
      tatgagaag agggtgcat tgtacccag gaggctgtg 
      tatcccttg tatacgcta gtttttatt ttttatgcc 
      ctgactttg attgttatg cttttgata aggccatgg 
      gtttgccgg aaatacctt ccaaggcag agcaaaatg 
      tcgatttat gccgccatg tactatata ccgattttg 
      gtggtggtc catgcccta attggggga cttctttat 
      tatgcgttc ccaattttg gtcgttatt ttgtcagtt 
      atttcgtgt gcttatcac tttgccctc aaaatcaac 
      caatcgatt ccctcactc atcataaca acagttgta 
      gacccgaga aatgtggta atcttagtg ggccattgg 
      tgtttacac gcctatggt ttaatttca ataactcag 
      cttagtgac ccgactttt cactcattg ttgctactg 
      ttggtccca ctgccggct tttttttac attttcacg 
      gccaggttt accgacccg acaatattt acgtaa
    mRNA sequence
    • >TC009612 RA
      tcgtattta acagtttta acaacaaat ctaccttat 
      gacacaaaa aaataccaa tttaccaca tttttaaac 
      aaataaatc caaacttgt aattttaat gagtcaaga 
      agagaatga aaaaatcac agtggacca accaaacat 
      tggtcaccc atcaacaga tcggcacga aactaacac 
      aactgcacc aaataatta aatcagcca aatttaatt 
      aaccaaacc ctaaaaatg tgccattgg cgcgcttgt 
      tatcgcatt gataaataa atgccccac tggccggct 
      gtaatctcc tgtcaagtc cgccttaac cttacattc 
      cgcgatgcc tctttaaga tgcttccgt gtcctggct 
      ggtactgcg gccgccagt acctcccca atggcagtc 
      tcagcgagt gcgggtcgt gcccccggg gcttccggc 
      gcaacgaca ctactttta tttgcgagc cgtgcaccg 
      acagcccca cgttctacg actggcttt atttggggt 
      ttatggtgc tcctggtcc agattctgc actggtttt 
      tcatcgaca tggtgtcga tgcgacgaa gcttcaaaa 
      aagacgtcc taattttac acttgactg cgctggtgg 
      aagtggtca tttcggggc tcttgtcgg tgcttttca 
      cgcacccgt tcggcgagt tccaggtgc ggaactgca 
      gagtgcgca gcttggccg attggtaca ctttgttgc 
      ataatccgg ccccggatt atgagaaga gggtgcatt 
      gtacccagg aggctgtgt atcccttgt atacgctag 
      tttttattt tttatgccc tgactttga ttgttatgc 
      ttttgataa ggccatggg tttgccgga aataccttc 
      caaggcaga gcaaaatgt cgatttatg ccgccatgt 
      actatatac cgattttgg tggtggtcc atgccctaa 
      ttgggggac ttctttatt atgcgttcc caattttgg 
      tcgttattt tgtcagtta tttcgtgtg cttatcact 
      ttgccctca aaatcaacc aatcgattc cctcactca 
      tcataacaa cagttgtag acccgagaa atgtggtaa 
      tcttagtgg gccattggt gtttacacg cctatggtt 
      taatttcaa taactcagc ttagtgacc cgacttttc 
      actcattgt tgctactgt tggtcccac tgccggctt 
      ttttttaca ttttcacgg ccaggttta ccgacccga 
      caatattta cgtaattta ttttttaat aataataat 
      aaactaaaa acagattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY