Status
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Gene information
General information
TC009607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3213816..3217943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009607
    iBeetle screen iB_04663

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009607 RA
      MDSGDDAIN NENVRRTRS GKIVRTPPA VKTPVKKTR 
      KKVVLMEVE VSEEESEEH NAIKTEPEQ NNGATIEEN 
      HEEVVEEAS EPAETSDME VSQEIETLV KEPEIKEEV 
      RVELETPEE TNGITEDID ETTEEKQVP SGEPDAENI 
      SEDELPTVQ AVKVPETEE VSDEELPGP KRAELPADT 
      EVVSEDELP TVKKEANKR KLVEGDYDP GSPTSEGET 
      PAKKASLEN TAVKEEEKN TKPKKLPEL DKYWKAVND 
      DPTDFTGWT YLLQYVDQE NDMEAAREA YDAFLSHYP 
      YCYGYWRKY ADYEKRKGN KKKCEEVFE RGLKAIPLS 
      VDLWIHYLT YVKTTKPDD EDYIRSQFE RAIAASGLE 
      FRSDRLWDS YIKWETEGK RLQHVTSIY DRLFTTPTQ 
      GYTTHFENF QEHITSNPP NKVVEVDEF LALRKEVRH 
      MLKHDAPNE TKTDNSDVP PGEDDASKA ISTDEETKA 
      IRERIISIR RKIHKNTVA AVTARWNFE EGIKRPYFH 
      VKPLERCQL KNWQEYLDF EIEQGDQVR IVVLFERCL 
      IACALYEEF WLKFVHYLE SLKDPELQP KIRDVYERA 
      CTIHHLKKP NLHLQWAMF EESVGNFNR AAEILVNLE 
      KSVPNVLQI AYRRINLER RRSDNEKCA QLYEHYINN 
      SKNKMISSN IAIKYSRFV LKVLKNTEK ALEILRSAI 
      TKDPNNPRL YLQLIDLTL QQDDVAENV IVELIDSFL 
      EKETADADQ KVLFAQRKL EYLEDFGAD IQSVQKAYD 
      DYQKYIKQS KETGKKKES KNDAASAGK KDKTSNQQT 
      SASSYGSYG SQTGQYNYS YGQGDQYQY QGWQYPQGS 
      YSGYNQWGS YGGGYSY
    CDS sequence
    • >TC009607 RA
      atggatagt ggtgacgac gcaatcaat aacgagaac 
      gtaaggaga acaagatcc ggtaaaata gtgcgtacg 
      cccccggcg gttaaaacc ccggtgaag aaaacgcga 
      aaaaaggtg gtcttgatg gaggtcgaa gtctcggaa 
      gaagagtcc gaagaacac aacgcgatt aaaaccgaa 
      cctgagcaa aacaacggc gccacaatt gaggaaaac 
      cacgaggag gtggttgag gaagcctcc gagccggcc 
      gaaacgtcc gacatggaa gttagtcaa gaaattgag 
      actcttgtt aaagagccc gaaattaaa gaagaggtt 
      agagtggag cttgagact ccggaggag accaacggt 
      attaccgaa gacattgac gaaacaact gaagaaaaa 
      caagtgcca agtggcgaa cctgatgcg gaaaacatc 
      tcggaggat gaattaccg acagttcaa gccgtgaaa 
      gtccccgag actgaggaa gtgtctgat gaggagttg 
      ccagggccg aaacgtgcc gaactcccg gccgatact 
      gaggtcgtg tcggaagac gagttaccg actgtgaag 
      aaagaagca aataaacgg aagttggta gaaggggat 
      tatgatcca ggttcgcca acgtcagaa ggcgaaact 
      ccagcgaag aaagcttcc ctggaaaat accgcagtt 
      aaagaagaa gagaagaac acaaaaccg aaaaaattg 
      cccgagttg gacaaatat tggaaggct gtgaacgat 
      gatcctact gattttacc gggtggact tatttgttg 
      caatacgtg gaccaggag aatgatatg gaggcggcg 
      cgcgaggcc tacgacgcg tttctgtcg cactatcca 
      tactgttat ggctactgg cggaagtat gccgattac 
      gagaagcgg aaaggcaac aagaaaaag tgcgaagag 
      gtattcgag agaggcttg aaagcgatc cccctttcg 
      gtcgatttg tggatccac tacctcacc tacgtcaaa 
      accaccaaa ccggatgat gaggactac atcaggtcg 
      cagtttgag agagccatc gctgccagt ggacttgaa 
      tttcgctca gatagactg tgggattct tacatcaaa 
      tgggaaacg gagggaaaa cgattacaa cacgtcacg 
      tcgatttat gacaggctt ttcacaacg ccaacgcaa 
      ggatacacc acacatttt gaaaatttc caagagcat 
      atcacgtca aatccacca aataaagtg gttgaagtt 
      gatgagttt ttggcgtta cggaaggaa gttaggcac 
      atgttgaaa catgatgct cctaacgaa accaaaacg 
      gacaattcg gacgtgcct ccaggtgaa gatgacgcc 
      agtaaagcc ataagtaca gatgaagaa accaaagcg 
      atacgagag aggattatt tcaattagg cgaaaaata 
      cacaagaat acggtggcg gcggtgacg gcgaggtgg 
      aattttgag gaaggaatc aaaaggccg tactttcac 
      gtgaagcct cttgagcgc tgccagtta aagaactgg 
      caagagtac ctggatttc gaaatcgaa caaggcgac 
      caagtcaga atcgtagtc ttgttcgag cgttgcctt 
      atagcctgt gctttgtac gaagaattt tggttaaaa 
      tttgttcac tatttggaa agcctgaaa gaccccgaa 
      ctccagccg aaaatccgc gatgtgtac gaacgcgcg 
      tgcaccatc caccatttg aaaaaaccc aacttgcac 
      ctgcaatgg gccatgttt gaggaaagc gttggcaat 
      tttaatcgg gccgctgaa atcctagta aatctcgaa 
      aagtcggtc cctaatgtc ttacaaatc gcttacaga 
      aggataaat ctggagagg aggcggagc gataacgag 
      aagtgtgca caactgtac gaacattac ataaacaat 
      tccaaaaat aagatgatt tcgagtaat atagctatt 
      aaatattcc cggtttgtg ctgaaagtg cttaagaat 
      accgaaaag gcgttggag attttgcgg tcggcgatt 
      acgaaagac ccgaataac cctagattg tacttgcag 
      ttgattgat ttgacgctg caacaggac gatgttgcg 
      gaaaatgtt atagtagag ttgattgat tccttcttg 
      gagaaggag acggctgac gcggaccaa aaggtgctt 
      tttgcgcaa cgaaagttg gagtatttg gaggatttc 
      ggcgcggat attcagtcg gtgcaaaag gcgtacgat 
      gactatcaa aagtacatc aagcagagt aaagagacg 
      ggcaagaag aaggagtcg aaaaatgat gccgcctcg 
      gcgggcaag aaagacaaa acatcgaac caacaaacc 
      tcagcctcg agttatggc agttacggt tcccaaaca 
      ggacagtac aattattcg tacgggcaa ggtgaccag 
      taccagtac caaggctgg cagtaccca caagggagt 
      tatagtggg tacaaccag tgggggtcg tatggagga 
      gggtacagt tactag
    mRNA sequence
    • >TC009607 RA
      ctcggcgtt tggtgagtg tcggaatta attttatcg 
      ataaaagcc aaaaaagtg gttaattac tccaaaaac 
      cgggcgtaa gtcttcaca aaaatggat agtggtgac 
      gacgcaatc aataacgag aacgtaagg agaacaaga 
      tccggtaaa atagtgcgt acgcccccg gcggttaaa 
      accccggtg aagaaaacg cgaaaaaag gtggtcttg 
      atggaggtc gaagtctcg gaagaagag tccgaagaa 
      cacaacgcg attaaaacc gaacctgag caaaacaac 
      ggcgccaca attgaggaa aaccacgag gaggtggtt 
      gaggaagcc tccgagccg gccgaaacg tccgacatg 
      gaagttagt caagaaatt gagactctt gttaaagag 
      cccgaaatt aaagaagag gttagagtg gagcttgag 
      actccggag gagaccaac ggtattacc gaagacatt 
      gacgaaaca actgaagaa aaacaagtg ccaagtggc 
      gaacctgat gcggaaaac atctcggag gatgaatta 
      ccgacagtt caagccgtg aaagtcccc gagactgag 
      gaagtgtct gatgaggag ttgccaggg ccgaaacgt 
      gccgaactc ccggccgat actgaggtc gtgtcggaa 
      gacgagtta ccgactgtg aagaaagaa gcaaataaa 
      cggaagttg gtagaaggg gattatgat ccaggttcg 
      ccaacgtca gaaggcgaa actccagcg aagaaagct 
      tccctggaa aataccgca gttaaagaa gaagagaag 
      aacacaaaa ccgaaaaaa ttgcccgag ttggacaaa 
      tattggaag gctgtgaac gatgatcct actgatttt 
      accgggtgg acttatttg ttgcaatac gtggaccag 
      gagaatgat atggaggcg gcgcgcgag gcctacgac 
      gcgtttctg tcgcactat ccatactgt tatggctac 
      tggcggaag tatgccgat tacgagaag cggaaaggc 
      aacaagaaa aagtgcgaa gaggtattc gagagaggc 
      ttgaaagcg atccccctt tcggtcgat ttgtggatc 
      cactacctc acctacgtc aaaaccacc aaaccggat 
      gatgaggac tacatcagg tcgcagttt gagagagcc 
      atcgctgcc agtggactt gaatttcgc tcagataga 
      ctgtgggat tcttacatc aaatgggaa acggaggga 
      aaacgatta caacacgtc acgtcgatt tatgacagg 
      cttttcaca acgccaacg caaggatac accacacat 
      tttgaaaat ttccaagag catatcacg tcaaatcca 
      ccaaataaa gtggttgaa gttgatgag tttttggcg 
      ttacggaag gaagttagg cacatgttg aaacatgat 
      gctcctaac gaaaccaaa acggacaat tcggacgtg 
      cctccaggt gaagatgac gccagtaaa gccataagt 
      acagatgaa gaaaccaaa gcgatacga gagaggatt 
      atttcaatt aggcgaaaa atacacaag aatacggtg 
      gcggcggtg acggcgagg tggaatttt gaggaagga 
      atcaaaagg ccgtacttt cacgtgaag cctcttgag 
      cgctgccag ttaaagaac tggcaagag tacctggat 
      ttcgaaatc gaacaaggc gaccaagtc agaatcgta 
      gtcttgttc gagcgttgc cttatagcc tgtgctttg 
      tacgaagaa ttttggtta aaatttgtt cactatttg 
      gaaagcctg aaagacccc gaactccag ccgaaaatc 
      cgcgatgtg tacgaacgc gcgtgcacc atccaccat 
      ttgaaaaaa cccaacttg cacctgcaa tgggccatg 
      tttgaggaa agcgttggc aattttaat cgggccgct 
      gaaatccta gtaaatctc gaaaagtcg gtccctaat 
      gtcttacaa atcgcttac agaaggata aatctggag 
      aggaggcgg agcgataac gagaagtgt gcacaactg 
      tacgaacat tacataaac aattccaaa aataagatg 
      atttcgagt aatatagct attaaatat tcccggttt 
      gtgctgaaa gtgcttaag aataccgaa aaggcgttg 
      gagattttg cggtcggcg attacgaaa gacccgaat 
      aaccctaga ttgtacttg cagttgatt gatttgacg 
      ctgcaacag gacgatgtt gcggaaaat gttatagta 
      gagttgatt gattccttc ttggagaag gagacggct 
      gacgcggac caaaaggtg ctttttgcg caacgaaag 
      ttggagtat ttggaggat ttcggcgcg gatattcag 
      tcggtgcaa aaggcgtac gatgactat caaaagtac 
      atcaagcag agtaaagag acgggcaag aagaaggag 
      tcgaaaaat gatgccgcc tcggcgggc aagaaagac 
      aaaacatcg aaccaacaa acctcagcc tcgagttat 
      ggcagttac ggttcccaa acaggacag tacaattat 
      tcgtacggg caaggtgac cagtaccag taccaaggc 
      tggcagtac ccacaaggg agttatagt gggtacaac 
      cagtggggg tcgtatgga ggagggtac agttactag 
      ttttagttc cactttcta gttttagga ttcgtttgt 
      acacttgta c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY