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Gene information
General information
TC009594 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3054437..3061897
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009594
    iBeetle screen iB_04662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009594 RA
      MASLVNGHS TQPGEMRNH PEDESEFQL EFTDDIHKT 
HMLASLNTM RKNRHFCDV ILHVGNTEV HAHRAVLAS 
ASPYLAELF ANDQAQNPL ENVVTFKLN GGFNKDALR 
ILVDYAYTG QLDVKYNQV KAVFLAANH LKMDRVTRT 
CAQHLIKHL SVENCIEIR SLPGIARNK EFIQQVDAF 
IAKEFDKIC KNNDVLNLH CAKIEVLNQ SREEMSLVN 
QNSLCRLVL EWIKRQVGE ESISVEMLS DKTFMLYLA 
IDNSLQDCS SLPTGDISD TEIVQDYKK MSLKNNNTN 
AKSKRKCLG QPSKPRVLI YNREIGEEI ELEGEPDWN 
LIASEKVGE HSFLALVTL GGRLSTLSI QLRLNTPST 
PSPVQTPDS SRPASEEKP DLYCALANM SCPRCSVGC 
ANFLNTLLV CGGYDRTEC LRTVEQYIP ETNTWKALP 
SMRENRGRF KIAVVNDKV YAIGGSNGT TELDSVEML 
DLSLDKWVK MPKLPLARS NMGVCHLDG LIYCIGGWN 
GQVGIKQCD VFDPVASEW SSIASLNTG RYQAGVTSY 
NKLVYAIGG CDAWNCLNS VEVYNPEEN TWSGIKPII 
TARRGCGVA VFNDKLYVV GGSDGSHSL SSTEIFDEK 
TQTWVVGPI MTTPRANVD VAVVGDRLY AVGGFSGKT 
FLNTIEYLD AKSNEWTTF VPKGSCEML RKSRSRHNS 
RRSMSEKTE SVERPSESF ES
    CDS sequence
    • >TC009594 RA
      atggcgtca ttagtgaac ggtcattcc actcagccc 
ggggaaatg cgaaaccac cccgaggac gagtccgaa 
ttccaactg gagttcacc gacgacatc cacaagacg 
catatgttg gcctccctc aacaccatg cgaaaaaat 
cgtcacttt tgtgacgtc attcttcac gttggcaat 
accgaagtt catgctcat agagctgta ctagcgagt 
gcttcaccc tatctcgcc gaacttttt gccaacgat 
caagcgcaa aatccccta gaaaatgtg gtcacgttt 
aaattgaac ggagggttt aataaagac gccttgagg 
atcctggtc gactatgca tacacaggc caactcgac 
gtcaagtat aaccaggtc aaggcggtt ttcctagcc 
gccaaccac ctaaagatg gaccgcgtg acccgaaca 
tgcgcccaa catttaata aaacacctc tctgtggaa 
aattgcatt gaaatccgt tccttgcct ggcattgcc 
cgcaacaag gaatttatc caacaagtg gacgcattt 
atcgctaaa gaatttgat aaaatttgc aaaaataac 
gatgtcctc aacttgcat tgcgccaaa atcgaggtt 
ttgaaccaa tcacgcgaa gaaatgtct ctagtgaac 
caaaattca ctttgtcgc ctggtcttg gaatggatc 
aaacgccaa gtgggcgaa gaatcaatt agtgttgaa 
atgttgtcc gataaaacc ttcatgttg tatctagca 
atcgataat tcgctacaa gattgctca agtcttccg 
actggcgac ataagcgat acggaaatc gtccaagac 
tataaaaaa atgtccctg aaaaacaac aacacgaac 
gctaaatcg aaacgcaaa tgtttgggc cagccgtca 
aaacctcgc gttctgatc tacaatcgc gaaattggc 
gaagagatc gaactcgaa ggcgaaccc gattggaat 
ttaatcgct tcggaaaaa gtaggcgag catagtttt 
ctcgcattg gtaacactg ggtggcagg ttgtcaaca 
ctgtcgatt cagttgcgt ttgaacaca ccttcgaca 
ccgtcacct gtgcaaact ccagattcg agtcggcca 
gcaagtgag gaaaagccg gacctgtac tgcgccctt 
gctaacatg tcgtgcccg cgatgctca gtcgggtgt 
gcaaatttc cttaatacg ctcctggtg tgcggtggc 
tacgaccgt actgagtgt ctccgtact gttgaacaa 
tacattcct gagactaac acttggaag gctttgcct 
tcaatgagg gagaataga gggcgcttt aaaatcgca 
gtagtgaac gataaagtc tacgctatt ggtgggagt 
aacggcact actgagctg gattcggtt gaaatgctc 
gacttgtca ttggataag tgggtcaaa atgcctaaa 
ttaccattg gctaggagt aatatgggg gtgtgtcat 
ctggacggt ttgatttat tgtattggg ggatggaat 
ggtcaagtt ggtattaag cagtgtgat gttttcgat 
cctgttgcg tccgagtgg agctccatt gcctcttta 
aacaccggg cgatatcaa gcgggcgtg acatcgtac 
aacaaactt gtctacgcc atcggcggc tgcgatgcc 
tggaattgc ctcaattcg gtcgaagtc tacaatccg 
gaggaaaac acttggagt ggcatcaaa ccgataatt 
acagcgcgt cgtggttgc ggcgttgcc gtttttaac 
gacaagttg tacgtagtt ggaggctct gacggctct 
cattccttg agttcgacc gaaattttc gatgaaaaa 
acccagact tgggtcgtg ggccccatt atgacgaca 
ccgcgcgct aatgtagat gtcgccgtg gtcggcgat 
cggctgtac gcagttggc gggttttca ggcaaaacg 
tttctgaat acgatcgag tatttggac gcgaaatcg 
aacgaatgg acgacgttt gtaccgaaa ggcagctgt 
gaaatgctc aggaaaagt cggagtcgg cacaactcc 
cgcaggagt atgtccgag aaaacggag agtgtcgag 
aggccttcg gaaagcttc gagtcataa
    mRNA sequence
    • >TC009594 RA
      cgcgctttc caatatggc agttgtatt catcggtca 
cgtttatta tgttatagt gctaaactt taacacccg 
cacaaccta acaacaatc cactcttat cacaaccac 
ccatcgctc cgagtgatc gtatttgga ccatggcgt 
cattagtga acggtcatt ccactcagc ccggggaaa 
tgcgaaacc accccgagg acgagtccg aattccaac 
tggagttca ccgacgaca tccacaaga cgcatatgt 
tggcctccc tcaacacca tgcgaaaaa atcgtcact 
tttgtgacg tcattcttc acgttggca ataccgaag 
ttcatgctc atagagctg tactagcga gtgcttcac 
cctatctcg ccgaacttt ttgccaacg atcaagcgc 
aaaatcccc tagaaaatg tggtcacgt ttaaattga 
acggagggt ttaataaag acgccttga ggatcctgg 
tcgactatg catacacag gccaactcg acgtcaagt 
ataaccagg tcaaggcgg ttttcctag ccgccaacc 
acctaaaga tggaccgcg tgacccgaa catgcgccc 
aacatttaa taaaacacc tctctgtgg aaaattgca 
ttgaaatcc gttccttgc ctggcattg cccgcaaca 
aggaattta tccaacaag tggacgcat ttatcgcta 
aagaatttg ataaaattt gcaaaaata acgatgtcc 
tcaacttgc attgcgcca aaatcgagg ttttgaacc 
aatcacgcg aagaaatgt ctctagtga accaaaatt 
cactttgtc gcctggtct tggaatgga tcaaacgcc 
aagtgggcg aagaatcaa ttagtgttg aaatgttgt 
ccgataaaa ccttcatgt tgtatctag caatcgata 
attcgctac aagattgct caagtcttc cgactggcg 
acataagcg atacggaaa tcgtccaag actataaaa 
aaatgtccc tgaaaaaca acaacacga acgctaaat 
cgaaacgca aatgtttgg gccagccgt caaaacctc 
gcgttctga tctacaatc gcgaaattg gcgaagaga 
tcgaactcg aaggcgaac ccgattgga atttaatcg 
cttcggaaa aagtaggcg agcatagtt ttctcgcat 
tggtaacac tgggtggca ggttgtcaa cactgtcga 
ttcagttgc gtttgaaca caccttcga caccgtcac 
ctgtgcaaa ctccagatt cgagtcggc cagcaagtg 
aggaaaagc cggacctgt actgcgccc ttgctaaca 
tgtcgtgcc cgcgatgct cagtcgggt gtgcaaatt 
tccttaata cgctcctgg tgtgcggtg gctacgacc 
gtactgagt gtctccgta ctgttgaac aatacattc 
ctgagacta acacttgga aggctttgc cttcaatga 
gggagaata gagggcgct ttaaaatcg cagtagtga 
acgataaag tctacgcta ttggtggga gtaacggca 
ctactgagc tggattcgg ttgaaatgc tcgacttgt 
cattggata agtgggtca aaatgccta aattaccat 
tggctagga gtaatatgg gggtgtgtc atctggacg 
gtttgattt attgtattg ggggatgga atggtcaag 
ttggtatta agcagtgtg atgttttcg atcctgttg 
cgtccgagt ggagctcca ttgcctctt taaacaccg 
ggcgatatc aagcgggcg tgacatcgt acaacaaac 
ttgtctacg ccatcggcg gctgcgatg cctggaatt 
gcctcaatt cggtcgaag tctacaatc cggaggaaa 
acacttgga gtggcatca aaccgataa ttacagcgc 
gtcgtggtt gcggcgttg ccgttttta acgacaagt 
tgtacgtag ttggaggct ctgacggct ctcattcct 
tgagttcga ccgaaattt tcgatgaaa aaacccaga 
cttgggtcg tgggcccca ttatgacga caccgcgcg 
ctaatgtag atgtcgccg tggtcggcg atcggctgt 
acgcagttg gcgggtttt caggcaaaa cgtttctga 
atacgatcg agtatttgg acgcgaaat cgaacgaat 
ggacgacgt ttgtaccga aaggcagct gtgaaatgc 
tcaggaaaa gtcggagtc ggcacaact cccgcagga 
gtatgtccg agaaaacgg agagtgtcg agaggcctt 
cggaaagct tcgagtcat aactagcat aatttcatg 
tctttagag taatcattg tctcacgcc taatcaggt 
cactctcat gttaatcaa gtgtaatca ttaggtaaa 
aagtattac tgaaaatac gtctcacga atcattaat 
tggggaaaa agactgcat ttgtttgtg ttcgtgact 
aaattacat aagcgcaac ttgaaaatt ccaagtctg 
actgaatga ttcttgagg cggtgcata taagtgtta 
acaccgaat tatcgtaga ttgtaaagt atcaactga 
ttgcgttga atttcccta cttttataa ttttagtcg 
gttcgtttc aaatttgta cgcaatttt ttatatgat 
ggagaaaaa gaatatatt ttaaatact cttttccac 
tgcaataac agaaattat ataaaaatg gaactttta 
acaataaag taaagtatt ataaagaca ctactatag 
gtcatgtta ctctattta ttagtaaat aatataaag 
tatgattgt tatatctgt aaatgtttg caacggctg 
gagcgcgcc cattgtgat ggataatat tgaaatatc 
tgcatattt tgccatatt tgtataatt gaagtggtt 
attggatga ataaatgtt tttcttttc t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY