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Gene information
General information
TC009594 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3054437..3061897
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009594
    iBeetle screen iB_04662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009594 RA
      MASLVNGHS TQPGEMRNH PEDESEFQL EFTDDIHKT 
      HMLASLNTM RKNRHFCDV ILHVGNTEV HAHRAVLAS 
      ASPYLAELF ANDQAQNPL ENVVTFKLN GGFNKDALR 
      ILVDYAYTG QLDVKYNQV KAVFLAANH LKMDRVTRT 
      CAQHLIKHL SVENCIEIR SLPGIARNK EFIQQVDAF 
      IAKEFDKIC KNNDVLNLH CAKIEVLNQ SREEMSLVN 
      QNSLCRLVL EWIKRQVGE ESISVEMLS DKTFMLYLA 
      IDNSLQDCS SLPTGDISD TEIVQDYKK MSLKNNNTN 
      AKSKRKCLG QPSKPRVLI YNREIGEEI ELEGEPDWN 
      LIASEKVGE HSFLALVTL GGRLSTLSI QLRLNTPST 
      PSPVQTPDS SRPASEEKP DLYCALANM SCPRCSVGC 
      ANFLNTLLV CGGYDRTEC LRTVEQYIP ETNTWKALP 
      SMRENRGRF KIAVVNDKV YAIGGSNGT TELDSVEML 
      DLSLDKWVK MPKLPLARS NMGVCHLDG LIYCIGGWN 
      GQVGIKQCD VFDPVASEW SSIASLNTG RYQAGVTSY 
      NKLVYAIGG CDAWNCLNS VEVYNPEEN TWSGIKPII 
      TARRGCGVA VFNDKLYVV GGSDGSHSL SSTEIFDEK 
      TQTWVVGPI MTTPRANVD VAVVGDRLY AVGGFSGKT 
      FLNTIEYLD AKSNEWTTF VPKGSCEML RKSRSRHNS 
      RRSMSEKTE SVERPSESF ES
    CDS sequence
    • >TC009594 RA
      atggcgtca ttagtgaac ggtcattcc actcagccc 
      ggggaaatg cgaaaccac cccgaggac gagtccgaa 
      ttccaactg gagttcacc gacgacatc cacaagacg 
      catatgttg gcctccctc aacaccatg cgaaaaaat 
      cgtcacttt tgtgacgtc attcttcac gttggcaat 
      accgaagtt catgctcat agagctgta ctagcgagt 
      gcttcaccc tatctcgcc gaacttttt gccaacgat 
      caagcgcaa aatccccta gaaaatgtg gtcacgttt 
      aaattgaac ggagggttt aataaagac gccttgagg 
      atcctggtc gactatgca tacacaggc caactcgac 
      gtcaagtat aaccaggtc aaggcggtt ttcctagcc 
      gccaaccac ctaaagatg gaccgcgtg acccgaaca 
      tgcgcccaa catttaata aaacacctc tctgtggaa 
      aattgcatt gaaatccgt tccttgcct ggcattgcc 
      cgcaacaag gaatttatc caacaagtg gacgcattt 
      atcgctaaa gaatttgat aaaatttgc aaaaataac 
      gatgtcctc aacttgcat tgcgccaaa atcgaggtt 
      ttgaaccaa tcacgcgaa gaaatgtct ctagtgaac 
      caaaattca ctttgtcgc ctggtcttg gaatggatc 
      aaacgccaa gtgggcgaa gaatcaatt agtgttgaa 
      atgttgtcc gataaaacc ttcatgttg tatctagca 
      atcgataat tcgctacaa gattgctca agtcttccg 
      actggcgac ataagcgat acggaaatc gtccaagac 
      tataaaaaa atgtccctg aaaaacaac aacacgaac 
      gctaaatcg aaacgcaaa tgtttgggc cagccgtca 
      aaacctcgc gttctgatc tacaatcgc gaaattggc 
      gaagagatc gaactcgaa ggcgaaccc gattggaat 
      ttaatcgct tcggaaaaa gtaggcgag catagtttt 
      ctcgcattg gtaacactg ggtggcagg ttgtcaaca 
      ctgtcgatt cagttgcgt ttgaacaca ccttcgaca 
      ccgtcacct gtgcaaact ccagattcg agtcggcca 
      gcaagtgag gaaaagccg gacctgtac tgcgccctt 
      gctaacatg tcgtgcccg cgatgctca gtcgggtgt 
      gcaaatttc cttaatacg ctcctggtg tgcggtggc 
      tacgaccgt actgagtgt ctccgtact gttgaacaa 
      tacattcct gagactaac acttggaag gctttgcct 
      tcaatgagg gagaataga gggcgcttt aaaatcgca 
      gtagtgaac gataaagtc tacgctatt ggtgggagt 
      aacggcact actgagctg gattcggtt gaaatgctc 
      gacttgtca ttggataag tgggtcaaa atgcctaaa 
      ttaccattg gctaggagt aatatgggg gtgtgtcat 
      ctggacggt ttgatttat tgtattggg ggatggaat 
      ggtcaagtt ggtattaag cagtgtgat gttttcgat 
      cctgttgcg tccgagtgg agctccatt gcctcttta 
      aacaccggg cgatatcaa gcgggcgtg acatcgtac 
      aacaaactt gtctacgcc atcggcggc tgcgatgcc 
      tggaattgc ctcaattcg gtcgaagtc tacaatccg 
      gaggaaaac acttggagt ggcatcaaa ccgataatt 
      acagcgcgt cgtggttgc ggcgttgcc gtttttaac 
      gacaagttg tacgtagtt ggaggctct gacggctct 
      cattccttg agttcgacc gaaattttc gatgaaaaa 
      acccagact tgggtcgtg ggccccatt atgacgaca 
      ccgcgcgct aatgtagat gtcgccgtg gtcggcgat 
      cggctgtac gcagttggc gggttttca ggcaaaacg 
      tttctgaat acgatcgag tatttggac gcgaaatcg 
      aacgaatgg acgacgttt gtaccgaaa ggcagctgt 
      gaaatgctc aggaaaagt cggagtcgg cacaactcc 
      cgcaggagt atgtccgag aaaacggag agtgtcgag 
      aggccttcg gaaagcttc gagtcataa
    mRNA sequence
    • >TC009594 RA
      cgcgctttc caatatggc agttgtatt catcggtca 
      cgtttatta tgttatagt gctaaactt taacacccg 
      cacaaccta acaacaatc cactcttat cacaaccac 
      ccatcgctc cgagtgatc gtatttgga ccatggcgt 
      cattagtga acggtcatt ccactcagc ccggggaaa 
      tgcgaaacc accccgagg acgagtccg aattccaac 
      tggagttca ccgacgaca tccacaaga cgcatatgt 
      tggcctccc tcaacacca tgcgaaaaa atcgtcact 
      tttgtgacg tcattcttc acgttggca ataccgaag 
      ttcatgctc atagagctg tactagcga gtgcttcac 
      cctatctcg ccgaacttt ttgccaacg atcaagcgc 
      aaaatcccc tagaaaatg tggtcacgt ttaaattga 
      acggagggt ttaataaag acgccttga ggatcctgg 
      tcgactatg catacacag gccaactcg acgtcaagt 
      ataaccagg tcaaggcgg ttttcctag ccgccaacc 
      acctaaaga tggaccgcg tgacccgaa catgcgccc 
      aacatttaa taaaacacc tctctgtgg aaaattgca 
      ttgaaatcc gttccttgc ctggcattg cccgcaaca 
      aggaattta tccaacaag tggacgcat ttatcgcta 
      aagaatttg ataaaattt gcaaaaata acgatgtcc 
      tcaacttgc attgcgcca aaatcgagg ttttgaacc 
      aatcacgcg aagaaatgt ctctagtga accaaaatt 
      cactttgtc gcctggtct tggaatgga tcaaacgcc 
      aagtgggcg aagaatcaa ttagtgttg aaatgttgt 
      ccgataaaa ccttcatgt tgtatctag caatcgata 
      attcgctac aagattgct caagtcttc cgactggcg 
      acataagcg atacggaaa tcgtccaag actataaaa 
      aaatgtccc tgaaaaaca acaacacga acgctaaat 
      cgaaacgca aatgtttgg gccagccgt caaaacctc 
      gcgttctga tctacaatc gcgaaattg gcgaagaga 
      tcgaactcg aaggcgaac ccgattgga atttaatcg 
      cttcggaaa aagtaggcg agcatagtt ttctcgcat 
      tggtaacac tgggtggca ggttgtcaa cactgtcga 
      ttcagttgc gtttgaaca caccttcga caccgtcac 
      ctgtgcaaa ctccagatt cgagtcggc cagcaagtg 
      aggaaaagc cggacctgt actgcgccc ttgctaaca 
      tgtcgtgcc cgcgatgct cagtcgggt gtgcaaatt 
      tccttaata cgctcctgg tgtgcggtg gctacgacc 
      gtactgagt gtctccgta ctgttgaac aatacattc 
      ctgagacta acacttgga aggctttgc cttcaatga 
      gggagaata gagggcgct ttaaaatcg cagtagtga 
      acgataaag tctacgcta ttggtggga gtaacggca 
      ctactgagc tggattcgg ttgaaatgc tcgacttgt 
      cattggata agtgggtca aaatgccta aattaccat 
      tggctagga gtaatatgg gggtgtgtc atctggacg 
      gtttgattt attgtattg ggggatgga atggtcaag 
      ttggtatta agcagtgtg atgttttcg atcctgttg 
      cgtccgagt ggagctcca ttgcctctt taaacaccg 
      ggcgatatc aagcgggcg tgacatcgt acaacaaac 
      ttgtctacg ccatcggcg gctgcgatg cctggaatt 
      gcctcaatt cggtcgaag tctacaatc cggaggaaa 
      acacttgga gtggcatca aaccgataa ttacagcgc 
      gtcgtggtt gcggcgttg ccgttttta acgacaagt 
      tgtacgtag ttggaggct ctgacggct ctcattcct 
      tgagttcga ccgaaattt tcgatgaaa aaacccaga 
      cttgggtcg tgggcccca ttatgacga caccgcgcg 
      ctaatgtag atgtcgccg tggtcggcg atcggctgt 
      acgcagttg gcgggtttt caggcaaaa cgtttctga 
      atacgatcg agtatttgg acgcgaaat cgaacgaat 
      ggacgacgt ttgtaccga aaggcagct gtgaaatgc 
      tcaggaaaa gtcggagtc ggcacaact cccgcagga 
      gtatgtccg agaaaacgg agagtgtcg agaggcctt 
      cggaaagct tcgagtcat aactagcat aatttcatg 
      tctttagag taatcattg tctcacgcc taatcaggt 
      cactctcat gttaatcaa gtgtaatca ttaggtaaa 
      aagtattac tgaaaatac gtctcacga atcattaat 
      tggggaaaa agactgcat ttgtttgtg ttcgtgact 
      aaattacat aagcgcaac ttgaaaatt ccaagtctg 
      actgaatga ttcttgagg cggtgcata taagtgtta 
      acaccgaat tatcgtaga ttgtaaagt atcaactga 
      ttgcgttga atttcccta cttttataa ttttagtcg 
      gttcgtttc aaatttgta cgcaatttt ttatatgat 
      ggagaaaaa gaatatatt ttaaatact cttttccac 
      tgcaataac agaaattat ataaaaatg gaactttta 
      acaataaag taaagtatt ataaagaca ctactatag 
      gtcatgtta ctctattta ttagtaaat aatataaag 
      tatgattgt tatatctgt aaatgtttg caacggctg 
      gagcgcgcc cattgtgat ggataatat tgaaatatc 
      tgcatattt tgccatatt tgtataatt gaagtggtt 
      attggatga ataaatgtt tttcttttc t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY