| General information | |||
| TC009594 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3054437..3061897 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009594 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04662 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC009594 RA
MASLVNGHS TQPGEMRNH PEDESEFQL EFTDDIHKT HMLASLNTM RKNRHFCDV ILHVGNTEV HAHRAVLAS ASPYLAELF ANDQAQNPL ENVVTFKLN GGFNKDALR ILVDYAYTG QLDVKYNQV KAVFLAANH LKMDRVTRT CAQHLIKHL SVENCIEIR SLPGIARNK EFIQQVDAF IAKEFDKIC KNNDVLNLH CAKIEVLNQ SREEMSLVN QNSLCRLVL EWIKRQVGE ESISVEMLS DKTFMLYLA IDNSLQDCS SLPTGDISD TEIVQDYKK MSLKNNNTN AKSKRKCLG QPSKPRVLI YNREIGEEI ELEGEPDWN LIASEKVGE HSFLALVTL GGRLSTLSI QLRLNTPST PSPVQTPDS SRPASEEKP DLYCALANM SCPRCSVGC ANFLNTLLV CGGYDRTEC LRTVEQYIP ETNTWKALP SMRENRGRF KIAVVNDKV YAIGGSNGT TELDSVEML DLSLDKWVK MPKLPLARS NMGVCHLDG LIYCIGGWN GQVGIKQCD VFDPVASEW SSIASLNTG RYQAGVTSY NKLVYAIGG CDAWNCLNS VEVYNPEEN TWSGIKPII TARRGCGVA VFNDKLYVV GGSDGSHSL SSTEIFDEK TQTWVVGPI MTTPRANVD VAVVGDRLY AVGGFSGKT FLNTIEYLD AKSNEWTTF VPKGSCEML RKSRSRHNS RRSMSEKTE SVERPSESF ES
- >TC009594 RA
atggcgtca ttagtgaac ggtcattcc actcagccc ggggaaatg cgaaaccac cccgaggac gagtccgaa ttccaactg gagttcacc gacgacatc cacaagacg catatgttg gcctccctc aacaccatg cgaaaaaat cgtcacttt tgtgacgtc attcttcac gttggcaat accgaagtt catgctcat agagctgta ctagcgagt gcttcaccc tatctcgcc gaacttttt gccaacgat caagcgcaa aatccccta gaaaatgtg gtcacgttt aaattgaac ggagggttt aataaagac gccttgagg atcctggtc gactatgca tacacaggc caactcgac gtcaagtat aaccaggtc aaggcggtt ttcctagcc gccaaccac ctaaagatg gaccgcgtg acccgaaca tgcgcccaa catttaata aaacacctc tctgtggaa aattgcatt gaaatccgt tccttgcct ggcattgcc cgcaacaag gaatttatc caacaagtg gacgcattt atcgctaaa gaatttgat aaaatttgc aaaaataac gatgtcctc aacttgcat tgcgccaaa atcgaggtt ttgaaccaa tcacgcgaa gaaatgtct ctagtgaac caaaattca ctttgtcgc ctggtcttg gaatggatc aaacgccaa gtgggcgaa gaatcaatt agtgttgaa atgttgtcc gataaaacc ttcatgttg tatctagca atcgataat tcgctacaa gattgctca agtcttccg actggcgac ataagcgat acggaaatc gtccaagac tataaaaaa atgtccctg aaaaacaac aacacgaac gctaaatcg aaacgcaaa tgtttgggc cagccgtca aaacctcgc gttctgatc tacaatcgc gaaattggc gaagagatc gaactcgaa ggcgaaccc gattggaat ttaatcgct tcggaaaaa gtaggcgag catagtttt ctcgcattg gtaacactg ggtggcagg ttgtcaaca ctgtcgatt cagttgcgt ttgaacaca ccttcgaca ccgtcacct gtgcaaact ccagattcg agtcggcca gcaagtgag gaaaagccg gacctgtac tgcgccctt gctaacatg tcgtgcccg cgatgctca gtcgggtgt gcaaatttc cttaatacg ctcctggtg tgcggtggc tacgaccgt actgagtgt ctccgtact gttgaacaa tacattcct gagactaac acttggaag gctttgcct tcaatgagg gagaataga gggcgcttt aaaatcgca gtagtgaac gataaagtc tacgctatt ggtgggagt aacggcact actgagctg gattcggtt gaaatgctc gacttgtca ttggataag tgggtcaaa atgcctaaa ttaccattg gctaggagt aatatgggg gtgtgtcat ctggacggt ttgatttat tgtattggg ggatggaat ggtcaagtt ggtattaag cagtgtgat gttttcgat cctgttgcg tccgagtgg agctccatt gcctcttta aacaccggg cgatatcaa gcgggcgtg acatcgtac aacaaactt gtctacgcc atcggcggc tgcgatgcc tggaattgc ctcaattcg gtcgaagtc tacaatccg gaggaaaac acttggagt ggcatcaaa ccgataatt acagcgcgt cgtggttgc ggcgttgcc gtttttaac gacaagttg tacgtagtt ggaggctct gacggctct cattccttg agttcgacc gaaattttc gatgaaaaa acccagact tgggtcgtg ggccccatt atgacgaca ccgcgcgct aatgtagat gtcgccgtg gtcggcgat cggctgtac gcagttggc gggttttca ggcaaaacg tttctgaat acgatcgag tatttggac gcgaaatcg aacgaatgg acgacgttt gtaccgaaa ggcagctgt gaaatgctc aggaaaagt cggagtcgg cacaactcc cgcaggagt atgtccgag aaaacggag agtgtcgag aggccttcg gaaagcttc gagtcataa
- >TC009594 RA
cgcgctttc caatatggc agttgtatt catcggtca cgtttatta tgttatagt gctaaactt taacacccg cacaaccta acaacaatc cactcttat cacaaccac ccatcgctc cgagtgatc gtatttgga ccatggcgt cattagtga acggtcatt ccactcagc ccggggaaa tgcgaaacc accccgagg acgagtccg aattccaac tggagttca ccgacgaca tccacaaga cgcatatgt tggcctccc tcaacacca tgcgaaaaa atcgtcact tttgtgacg tcattcttc acgttggca ataccgaag ttcatgctc atagagctg tactagcga gtgcttcac cctatctcg ccgaacttt ttgccaacg atcaagcgc aaaatcccc tagaaaatg tggtcacgt ttaaattga acggagggt ttaataaag acgccttga ggatcctgg tcgactatg catacacag gccaactcg acgtcaagt ataaccagg tcaaggcgg ttttcctag ccgccaacc acctaaaga tggaccgcg tgacccgaa catgcgccc aacatttaa taaaacacc tctctgtgg aaaattgca ttgaaatcc gttccttgc ctggcattg cccgcaaca aggaattta tccaacaag tggacgcat ttatcgcta aagaatttg ataaaattt gcaaaaata acgatgtcc tcaacttgc attgcgcca aaatcgagg ttttgaacc aatcacgcg aagaaatgt ctctagtga accaaaatt cactttgtc gcctggtct tggaatgga tcaaacgcc aagtgggcg aagaatcaa ttagtgttg aaatgttgt ccgataaaa ccttcatgt tgtatctag caatcgata attcgctac aagattgct caagtcttc cgactggcg acataagcg atacggaaa tcgtccaag actataaaa aaatgtccc tgaaaaaca acaacacga acgctaaat cgaaacgca aatgtttgg gccagccgt caaaacctc gcgttctga tctacaatc gcgaaattg gcgaagaga tcgaactcg aaggcgaac ccgattgga atttaatcg cttcggaaa aagtaggcg agcatagtt ttctcgcat tggtaacac tgggtggca ggttgtcaa cactgtcga ttcagttgc gtttgaaca caccttcga caccgtcac ctgtgcaaa ctccagatt cgagtcggc cagcaagtg aggaaaagc cggacctgt actgcgccc ttgctaaca tgtcgtgcc cgcgatgct cagtcgggt gtgcaaatt tccttaata cgctcctgg tgtgcggtg gctacgacc gtactgagt gtctccgta ctgttgaac aatacattc ctgagacta acacttgga aggctttgc cttcaatga gggagaata gagggcgct ttaaaatcg cagtagtga acgataaag tctacgcta ttggtggga gtaacggca ctactgagc tggattcgg ttgaaatgc tcgacttgt cattggata agtgggtca aaatgccta aattaccat tggctagga gtaatatgg gggtgtgtc atctggacg gtttgattt attgtattg ggggatgga atggtcaag ttggtatta agcagtgtg atgttttcg atcctgttg cgtccgagt ggagctcca ttgcctctt taaacaccg ggcgatatc aagcgggcg tgacatcgt acaacaaac ttgtctacg ccatcggcg gctgcgatg cctggaatt gcctcaatt cggtcgaag tctacaatc cggaggaaa acacttgga gtggcatca aaccgataa ttacagcgc gtcgtggtt gcggcgttg ccgttttta acgacaagt tgtacgtag ttggaggct ctgacggct ctcattcct tgagttcga ccgaaattt tcgatgaaa aaacccaga cttgggtcg tgggcccca ttatgacga caccgcgcg ctaatgtag atgtcgccg tggtcggcg atcggctgt acgcagttg gcgggtttt caggcaaaa cgtttctga atacgatcg agtatttgg acgcgaaat cgaacgaat ggacgacgt ttgtaccga aaggcagct gtgaaatgc tcaggaaaa gtcggagtc ggcacaact cccgcagga gtatgtccg agaaaacgg agagtgtcg agaggcctt cggaaagct tcgagtcat aactagcat aatttcatg tctttagag taatcattg tctcacgcc taatcaggt cactctcat gttaatcaa gtgtaatca ttaggtaaa aagtattac tgaaaatac gtctcacga atcattaat tggggaaaa agactgcat ttgtttgtg ttcgtgact aaattacat aagcgcaac ttgaaaatt ccaagtctg actgaatga ttcttgagg cggtgcata taagtgtta acaccgaat tatcgtaga ttgtaaagt atcaactga ttgcgttga atttcccta cttttataa ttttagtcg gttcgtttc aaatttgta cgcaatttt ttatatgat ggagaaaaa gaatatatt ttaaatact cttttccac tgcaataac agaaattat ataaaaatg gaactttta acaataaag taaagtatt ataaagaca ctactatag gtcatgtta ctctattta ttagtaaat aatataaag tatgattgt tatatctgt aaatgtttg caacggctg gagcgcgcc cattgtgat ggataatat tgaaatatc tgcatattt tgccatatt tgtataatt gaagtggtt attggatga ataaatgtt tttcttttc t
