Status
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Gene information
General information
TC009582 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2862101..2879756
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009582
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04660

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009582 RA
      MQQRGLYAT MNIENEDKS SQVSRGRHN SKLKVLVRS 
      HAIREEASP PRESSPSLL ISPSIKVIP STETSPASS 
      SPSGNPVVV LSPSLTSSP CLSPVCVSP TSTLVPSPT 
      NADASDDNN SPKYLNRLN NKQKENASQ NNRKKYICD 
      CPNQNCKCA KNQERNFNN NLTNINNNV VSSNTLTVS 
      RVNSRNKLR QQSSSQGSF EGSSSNSPC LSRDSSSEQ 
      YTDTSGADL EVFIPETLN RNAKDRAFM LRIEQELVS 
      LAKDKVKTH YKFPPMSSY QRMLVHRCA AYFGMDHNI 
      ESSGKCVVV NKTKNTRIP DVEFKEHIK EDVVFSEEP 
      RRSILKRDS NSIEDYSFK SPDRQYSME NRRSKSFEE 
      REEEYAKVR RRIFNKEEL CLLRQMQDG GSSEDFEWA 
      EIPWSSTDS ENSSRFRLQ LPEHGRRAG KLTKVLSEE 
      VGETSRPYV AKSYSFGGY AGVLNRGDS VVSTHSAGP 
      RLLTKQDSG ASSVSWRLS PSSSGYKSQ SQMSESITP 
      SPTSTPNQI VESSRQDDE KDSSDSRIV WAVTDIQRV 
      PKGSIIINP QTGEPIKNE DGSLYHYDP NNPPPGFVT 
      ASKPPPSPK KTSPQSVSP KKRISKLSP SHKSHVTNS 
      STSPSLPYS PPPNRTFPF MGDNNMGGY PVYGPGYPP 
      SGHETVPYY QQPCIVYTA PYNVPISPH FEGRMEPPP 
      MNEMGGTYY VPDNNITYQ PQPSYYQQP ITFYPNAPP 
      PNPPPPRFP VHHYQPPPP PPNPDLVPV YPHLVYPNQ 
      GYQPPNQPN VVYAQNPVY YPPYPQNTS TPNSCASSV 
      SHTQFSSHL QENNHQNAV MQQLTQNMS QMSFVQSQN 
      HSNYNNMLN KMTPPQFDV RQKSNKFGA TPKGFVLGS 
      SQSSTGTNS PAATVVAGY CPQNPGMYR TPPETPPNQ 
      YNYGPGFPI PPMLFRQMS NVRANTPGT TRSSRSPTP 
      AGDVAHFDR PRIAPPGVY HGMPYVLPS DSRGIPGSN 
      QYRQPPPPV KQHPFSQTS DKSHKNRKS KGNKLSSLP 
      NAK
    CDS sequence
    • >TC009582 RA
      atgcagcag agaggatta tacgcgacc atgaatatc 
      gaaaacgag gataaatcg tcacaagtg agccgaggg 
      cggcacaat tcgaaatta aaagtcttg gtccgaagt 
      cacgccatc cgggaggaa gcttcccct cctcgcgaa 
      tcctctccc tccctcctg atctccccc agcatcaaa 
      gtcattccc tcgacggaa acctctcct gccagctct 
      tcgccttcg gggaatccc gtcgttgtg ttgagtccg 
      tcgttgacg tcatcgccg tgtctctcc cccgtctgt 
      gtttccccg acttcgacg ctcgtgccg tcgccgacg 
      aatgcggac gcgagcgac gacaacaac agcccgaaa 
      tacctcaat cgactcaac aacaaacag aaggaaaat 
      gctagtcag aacaatagg aagaagtac atttgtgat 
      tgtccgaac cagaactgc aagtgcgcc aagaaccag 
      gagcgcaat tttaacaac aatctcacg aatattaac 
      aacaatgtg gtcagttcc aacactttg actgttagt 
      agggtgaat tcgaggaac aagctgcgg cagcagagc 
      tcgtcccag gggagcttc gagggcagc tcgtccaat 
      tcgccctgt ttgtccaga gacagcagt tcggagcaa 
      tacacggac acttccggt gccgatctc gaggtgttc 
      atacctgaa accctcaac agaaacgcc aaggaccgg 
      gcgttcatg ctgaggatc gaacaagag ctagtcagt 
      cttgccaaa gataaagtt aaaactcat tacaaattc 
      ccgccgatg tcgagctac caacgcatg ctggtccat 
      cgttgcgcc gcttacttc ggcatggac cacaacatc 
      gaatcgtcc ggcaagtgc gtggtggtc aacaaaacg 
      aaaaacacc cgaataccg gacgtcgaa ttcaaggag 
      cacatcaaa gaggacgtc gtcttcagc gaggagccc 
      agacgttcg atcctcaag cgtgactca aacagcatt 
      gaggactac agtttcaag tcgccggat cgccaatac 
      tccatggag aacaggcgg agtaaaagt ttcgaggaa 
      cgagaagag gagtacgcg aaagtacgc cgacgaatt 
      ttcaacaaa gaagagttg tgtctcttg cgccaaatg 
      caagacggc ggcagcagt gaggacttc gagtgggct 
      gagataccg tggtcgagc accgatagt gaaaattcg 
      tccaggttt cggctccaa ttgcctgaa catggacga 
      agagctgga aaattaact aaagttctc tctgaggag 
      gttggcgag acttcgagg ccttatgtc gccaaaagt 
      tacagtttt gggggctat gctggggtc ttgaacagg 
      ggggacagt gtcgtgtct acgcacagt gccgggccc 
      agactccta accaaacaa gactcgggt gccagttcg 
      gtgtcatgg cgcctcagc ccgtcaagt tctggctac 
      aaatctcag tctcaaatg tccgagtct ataaccccc 
      tcccccacc tccaccccc aaccagatc gtggaatcg 
      agtcgtcaa gacgacgaa aaagactcg agcgattcc 
      cgaatcgtg tgggcggtg actgatata cagagagtc 
      ccaaaaggc agtattatt ataaaccca caaaccggg 
      gaaccaatc aaaaacgag gacgggtca ctgtaccac 
      tacgacccg aataacccg ccccctggg tttgtcacc 
      gcctccaaa ccgccccct tcaccgaaa aaaacctca 
      ccacaaagt gtatcaccg aaaaaacga atttcgaaa 
      ctctcgcct agtcataaa tcccacgtg actaacagt 
      tcgacaagt cccagtttg ccttactcc cctccacca 
      aatcggacc ttccccttt atgggtgat aacaacatg 
      ggtgggtac ccagtttat gggcccgga tatccaccg 
      tcgggccac gaaactgtc ccatactac caacaacca 
      tgcatcgta tacaccgcc ccttacaac gtacccatc 
      tcgccccat ttcgaagga cgaatggaa cctccacca 
      atgaacgaa atgggcggt acttactac gtcccggat 
      aacaacata acatatcaa ccgcaaccc tcctactac 
      caacaacca atcacgttt tacccgaac gctccgccc 
      ccgaacccg cccccgccg cgattccca gtccaccac 
      taccagccc ccgcccccg ccgcccaac cctgacctg 
      gttccagtc tacccccac cttgtctat cccaatcag 
      gggtaccaa ccccccaac caaccaaat gttgtttac 
      gcacaaaac cccgtctac taccccccc tacccccaa 
      aacacctcc acccccaac agttgcgcc tcctccgtc 
      tcccacacg caattctcg agccacctc caggaaaac 
      aaccaccag aacgctgtg atgcaacaa ctcacgcaa 
      aatatgtca caaatgagt tttgtacaa agccagaat 
      cattcgaat tataataac atgttgaat aagatgacg 
      ccgccccaa tttgacgtc agacaaaaa agcaataaa 
      ttcggggca accccgaaa ggttttgtc ttggggtct 
      agtcagagt agcactggg acgaattca ccggcggcg 
      actgtggtg gccgggtac tgcccccaa aatccgggg 
      atgtacaga acgcccccg gaaacgccc cctaatcag 
      tacaactac gggccgggg tttcccata ccgccgatg 
      cttttccga caaatgagc aatgttagg gctaacact 
      cccggtacg acccgaagt agtcggtct ccaacaccg 
      gccggtgac gtggcgcat tttgatagg ccgagaatt 
      gcgccacct ggtgtctac catggcatg ccttacgtc 
      cttccatct gatagtcgg gggattccc gggagtaat 
      cagtaccgg cagccgcct ccgccggta aaacagcac 
      ccgttcagc cagacgtct gacaagtca cacaaaaat 
      cgaaagtca aagggaaac aaactgtca agtttgccc 
      aacgcaaaa taa
    mRNA sequence
    • >TC009582 RA
      ttgggtttg gaaaccaaa cgataggtt ccaaaaagt 
      tgtgttatt gaggcattg tgttacgaa agtaaaatt 
      ccgggtgct tgtaagtag gaaaaagcg cgttttttg 
      tggcgtttg tcggtcaca aggcgtttt tttgtcgtg 
      tttgaaaat cgcgttttt tgtagaaat tcgtctcct 
      ttgtgcagt ttttgtttt tttttgtgt gatggtatg 
      aagcacggg tgcccccat ggattatag agtgttggt 
      tgtggtcgg ctgaataac gcttcagga cgcgaaaaa 
      tggagtttg gagcgtgtc ggtgagtcc ccgaatttt 
      cgcacttcg tgctattga actgttttt gcagttgcc 
      ataatttcg ggggtttca gttaacaca atgcagcag 
      agaggatta tacgcgacc atgaatatc gaaaacgag 
      gataaatcg tcacaagtg agccgaggg cggcacaat 
      tcgaaatta aaagtcttg gtccgaagt cacgccatc 
      cgggaggaa gcttcccct cctcgcgaa tcctctccc 
      tccctcctg atctccccc agcatcaaa gtcattccc 
      tcgacggaa acctctcct gccagctct tcgccttcg 
      gggaatccc gtcgttgtg ttgagtccg tcgttgacg 
      tcatcgccg tgtctctcc cccgtctgt gtttccccg 
      acttcgacg ctcgtgccg tcgccgacg aatgcggac 
      gcgagcgac gacaacaac agcccgaaa tacctcaat 
      cgactcaac aacaaacag aaggaaaat gctagtcag 
      aacaatagg aagaagtac atttgtgat tgtccgaac 
      cagaactgc aagtgcgcc aagaaccag gagcgcaat 
      tttaacaac aatctcacg aatattaac aacaatgtg 
      gtcagttcc aacactttg actgttagt agggtgaat 
      tcgaggaac aagctgcgg cagcagagc tcgtcccag 
      gggagcttc gagggcagc tcgtccaat tcgccctgt 
      ttgtccaga gacagcagt tcggagcaa tacacggac 
      acttccggt gccgatctc gaggtgttc atacctgaa 
      accctcaac agaaacgcc aaggaccgg gcgttcatg 
      ctgaggatc gaacaagag ctagtcagt cttgccaaa 
      gataaagtt aaaactcat tacaaattc ccgccgatg 
      tcgagctac caacgcatg ctggtccat cgttgcgcc 
      gcttacttc ggcatggac cacaacatc gaatcgtcc 
      ggcaagtgc gtggtggtc aacaaaacg aaaaacacc 
      cgaataccg gacgtcgaa ttcaaggag cacatcaaa 
      gaggacgtc gtcttcagc gaggagccc agacgttcg 
      atcctcaag cgtgactca aacagcatt gaggactac 
      agtttcaag tcgccggat cgccaatac tccatggag 
      aacaggcgg agtaaaagt ttcgaggaa cgagaagag 
      gagtacgcg aaagtacgc cgacgaatt ttcaacaaa 
      gaagagttg tgtctcttg cgccaaatg caagacggc 
      ggcagcagt gaggacttc gagtgggct gagataccg 
      tggtcgagc accgatagt gaaaattcg tccaggttt 
      cggctccaa ttgcctgaa catggacga agagctgga 
      aaattaact aaagttctc tctgaggag gttggcgag 
      acttcgagg ccttatgtc gccaaaagt tacagtttt 
      gggggctat gctggggtc ttgaacagg ggggacagt 
      gtcgtgtct acgcacagt gccgggccc agactccta 
      accaaacaa gactcgggt gccagttcg gtgtcatgg 
      cgcctcagc ccgtcaagt tctggctac aaatctcag 
      tctcaaatg tccgagtct ataaccccc tcccccacc 
      tccaccccc aaccagatc gtggaatcg agtcgtcaa 
      gacgacgaa aaagactcg agcgattcc cgaatcgtg 
      tgggcggtg actgatata cagagagtc ccaaaaggc 
      agtattatt ataaaccca caaaccggg gaaccaatc 
      aaaaacgag gacgggtca ctgtaccac tacgacccg 
      aataacccg ccccctggg tttgtcacc gcctccaaa 
      ccgccccct tcaccgaaa aaaacctca ccacaaagt 
      gtatcaccg aaaaaacga atttcgaaa ctctcgcct 
      agtcataaa tcccacgtg actaacagt tcgacaagt 
      cccagtttg ccttactcc cctccacca aatcggacc 
      ttccccttt atgggtgat aacaacatg ggtgggtac 
      ccagtttat gggcccgga tatccaccg tcgggccac 
      gaaactgtc ccatactac caacaacca tgcatcgta 
      tacaccgcc ccttacaac gtacccatc tcgccccat 
      ttcgaagga cgaatggaa cctccacca atgaacgaa 
      atgggcggt acttactac gtcccggat aacaacata 
      acatatcaa ccgcaaccc tcctactac caacaacca 
      atcacgttt tacccgaac gctccgccc ccgaacccg 
      cccccgccg cgattccca gtccaccac taccagccc 
      ccgcccccg ccgcccaac cctgacctg gttccagtc 
      tacccccac cttgtctat cccaatcag gggtaccaa 
      ccccccaac caaccaaat gttgtttac gcacaaaac 
      cccgtctac taccccccc tacccccaa aacacctcc 
      acccccaac agttgcgcc tcctccgtc tcccacacg 
      caattctcg agccacctc caggaaaac aaccaccag 
      aacgctgtg atgcaacaa ctcacgcaa aatatgtca 
      caaatgagt tttgtacaa agccagaat cattcgaat 
      tataataac atgttgaat aagatgacg ccgccccaa 
      tttgacgtc agacaaaaa agcaataaa ttcggggca 
      accccgaaa ggttttgtc ttggggtct agtcagagt 
      agcactggg acgaattca ccggcggcg actgtggtg 
      gccgggtac tgcccccaa aatccgggg atgtacaga 
      acgcccccg gaaacgccc cctaatcag tacaactac 
      gggccgggg tttcccata ccgccgatg cttttccga 
      caaatgagc aatgttagg gctaacact cccggtacg 
      acccgaagt agtcggtct ccaacaccg gccggtgac 
      gtggcgcat tttgatagg ccgagaatt gcgccacct 
      ggtgtctac catggcatg ccttacgtc cttccatct 
      gatagtcgg gggattccc gggagtaat cagtaccgg 
      cagccgcct ccgccggta aaacagcac ccgttcagc 
      cagacgtct gacaagtca cacaaaaat cgaaagtca 
      aagggaaac aaactgtca agtttgccc aacgcaaaa 
      taattctaa attaattgt tacgaaaca aacacacgt 
      gggttttac atcgatttc gtgctgccc tgcaattgt 
      tgtaaaaat cgaggtaag aagttcctt ttaatttgt 
      agacatcac ttatttatt tagcgattc gatgccata 
      atgagatta cgaaaggat ttgtttgcc gtaacgtaa 
      atatgaata aaaattgct caattcgtt tcgttgttt 
      cgcacaatg ttgtttttg ttgttacgt ttgagaagt 
      cttacctag agacgtcgt gcgttgact gggcctctt 
      gatacgaaa agacaatct attttgttg tttgtatgt 
      gaaactttg taaatactg aagggtcca ttttaggtg 
      tacacattt ttgtatttt ggaaaactt gcctgaaat 
      gtgttcaac taaaaccaa caacacaag gaaacaata 
      actgacact ttttttccc tttaaatgt ggcgacaac 
      tgttgtttg taaattgta ttgaattta ttaagaaat 
      tttataaaa actgttgaa tgttttcct attttgggt 
      caacaattt taagcatgg acagttgag tgatttttt 
      gtgattcgt ttttgacta agtttgttg agccgttgt 
      ttatatttt acgcagaat tgttgatct aaaatgatt 
      tcgtaacgg attttttac cgttcaagg aattccatc 
      catttctag tactttttc caaggtaat tttacacac 
      tccatcatt caaattgtc atttttcga gtcaattac 
      gatacatac aagacaaaa ctttaccga tttatctga 
      tgtgtactt aatttttat gtaaaagca gtttctttg 
      gaaaactga tgactatgt cgtgcttcg gacatcaga 
      tttccaacg gtaaaatgg agaaacaaa catgtacat 
      cgttttaaa attgatatt ttcaaagta gtattaatc 
      gttgtagaa gttaggcgt taagttgta tttccaaca 
      taggttttt tatgtttgt aatgatttt atttattat 
      ttgtgttct aatgtgcct cggacactt gtaaattaa 
      agttatttc ataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY