| General information | |||
| TC031184
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2653191..2752693 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031184 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04657 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC031184 RA
MVAPVFFAQ LCVLLVCGA DYANAGKDV PTSHIEVVA GEPVYLPCD ISTGDPSDQ VLLVLWYRE DLGTPIYSV DGRDRDFSL AERWSDENV FANRAYFMP EKQPAELGV DHIRETDQG VYRCRVDFK VAQTRNSKV NLTVIVPPQ KIVITNESG AQLESVVGP FPEGASFTL RCDVFGAKP LPSVTWFRN DVVVSNVSV ALPRAGTTH VRSELRITG LGRRDVHSE LTCQASNNP KTPPLASTL HVDMNFGPL DVKILGANQ PLSAGRRYD LLCQSSGSR PPASITWWR NGQRLEGAK KTTSNDGNT TTSTLSFVP RKEDDGKYL SCRAENKMM STDRLEDKW KLEIHYTPE AKIILGTSL NPDSIREGT DVYFDCVIN AHPPVYKVE WKHNGKALN INIQAGIII TNQSLVLQG VSRSTAGNY TCVGYNTEG DGESNPFYL NVMYAPTCK PNQSRIYGV AKQEQAQIT CQVDANPPD VQFRWTFNN SADSVDVAQ SHIARSGTS SVVSYTPMT ELDYGTLLC YASNRIGTQ RVPCVFHII AAGRPDQVH NCTLSNISM TSFSIRCSE GFNGGLPQS FLLEVRESH SQEVKSNLS SPVPRFSVT TLEAGAQYQ AALFSYNLK GRSEPVILQ AATLRLPEK QLTAEKDNH RSAFRFTPM MSVLIGIVS ALLIVGLVV ILLVRLQCT QNDDRRKRH KNATATGGN LEHRGSTSG PTLSDKGGG SPISKHDSS AGECDSDEK NPDIIPQPV DTDETADYV RKRQHISTI ETSPSRSLL HQAGNSAGG GGGGNAGYI GYCTLRNGM PLHELAAQS KMHAAPPPM MGGYAPCTL PRQPQQWQY GPVSHLPPG VYPVPYRGP HPQQRPRPQ PREPPIPMQ PLATTEEEP SVDTPLMVN KRESTV
- >TC031184 RA
atggtggcc ccggtcttt tttgcccag ttgtgtgtg ttgcttgtt tgcggggcg gattatgct aatgccggc aaagacgtt cccacgagt cacatcgag gtggtggct ggtgagcct gtgtatctg ccttgcgac atctccacc ggggacccc agcgaccag gttttgctg gtgttgtgg tacagggaa gatttaggg acccccata tacagtgtg gacggcagg gatcgggat ttcagtctg gccgagcgt tggtccgat gaaaatgtg tttgccaac agggcgtac ttcatgccc gagaagcaa ccagctgag ttaggggtg gaccacatc agggagacg gaccagggg gtctacaga tgcagagtc gattttaaa gtggcgcag acgaggaac agcaaagtc aacctcacg gttatcgtg cccccgcaa aaaatcgtc atcaccaac gagtcgggg gcgcagctg gagtccgtg gtggggccc tttcccgag ggcgcctcc ttcacgctg cgctgtgac gtctttgga gccaagcct ctccctagt gtgacatgg ttccgtaac gatgtggtg gtgagcaac gtcagtgtg gctttacca cgagctgga acgacgcac gttcgtagc gagttgcgg ataacaggt ctgggacgt cgcgacgtc cactccgaa ctcacctgt caggcgtcc aataatccg aaaacaccc cctcttgcc tccaccctc cacgtagac atgaatttc ggtccgctc gatgtcaag atcctggga gccaaccag ccgctttcc gccggaagg agatacgat ttgctgtgt cagagttcc gggtcaaga cctccggcc agcatcacc tggtggagg aacgggcag cggctcgaa ggggccaaa aagacgacg tccaacgac ggcaacaca accaccagc actttatca tttgtaccg agaaaagag gatgacgga aaatatcta tcatgcaga gccgaaaac aaaatgatg tctacagac agactcgag gataaatgg aaattagaa atacactat acccccgag gcgaagata attctgggc acttcgctc aatccggat tccattcgg gaaggaaca gacgtctat tttgactgc gtcatcaac gcacatcct cccgtctac aaagtcgag tggaaacac aacggcaag gcgctcaat atcaacata caagcgggg atcatcatc acaaaccaa agcctggtg ctgcaaggg gtctccaga tcgacggca ggaaattac acctgcgtg gggtacaac acggagggg gacggcgag agcaacccc ttctatctc aacgtcatg tacgcgccg acgtgcaag ccgaaccag agcagaatt tacggtgtc gcgaaacaa gaacaagca caaataacg tgtcaagtc gatgcaaat cctcccgac gtccaattt cgatggacg tttaataat tctgcagat tccgtcgac gtggcccaa tcgcatatt gccagaagt ggaacgtcg agtgtcgtt tcttacact ccgatgact gaactcgat tacgggacg cttttgtgt tacgcttcg aatagaatt gggacgcaa cgagtgccc tgcgtgttc cacatcatt gcagcagga cgccctgac caagtccac aactgtacg ctatccaac atctccatg acgtccttt tccattaga tgttcggag ggttttaat ggcggtttg ccgcagtcc tttctatta gaggttcgc gaaagtcac agccaggag gtaaaaagc aacctctca tcccctgtt ccgagattt tcagtgaca actttagag gcgggcgcc caataccag gctgccttg ttctcgtac aacttgaaa ggacgaagt gaaccggtc atcttacaa gctgccact ctacgactt cctgaaaaa caactcact gccgaaaaa gacaaccac cggtccgcc tttcgtttt acgccgatg atgagtgtc ctaattgga atcgtctca gcgcttttg attgtcgga ctcgtggtg attttgctg gtgcgcttg caatgcact caaaacgac gacagaagg aaaagacac aaaaatgca acggctacg ggaggaaat ctcgaacac agaggctcc accagcgga ccgacgtta agcgacaag ggaggtggc agtccaata tcgaaacac gattcaagc gcaggcgaa tgcgacagc gacgagaag aaccccgac ataattccg cagcccgtc gacaccgac gagaccgct gattacgtc aggaaaaga caacacatt agcacaatc gaaacatct cccagtcgg agtctttta catcaagcc ggcaattca gcagggggc ggcgggggc ggcaacgcc ggttacatc ggttactgc acacttcgc aacggaatg cccttacac gaactcgcc gcacaatcg aaaatgcac gctgcgccg cccccgatg atggggggc tacgccccc tgcacgctg ccccgccag ccgcagcag tggcagtac ggccccgtg agccacctg cccccgggg gtgtacccg gtgccgtac cgcggcccc catccgcag cagaggccg cgtccgcag ccccgggag ccccccata cccatgcag cccctcgcc acgacggaa gaggagccc tcggtggac acccccctc atggtgaac aagcgggaa agcacggtt tga
- >TC031184 RA
tatgacgaa agcgtcatc agcgttggc caagagatc gataataaa ttagtttcg cacgatttt tccggtggc gatttgtag gggaggccg ggcctaagc cgagttcgt aaagtaaat gttttggta gttgggtcg gaggagttg agtggtggc cccaggccc cagatgcgt ctccaagga ttgcgactt ccggttatg acttgaagt gtgagtgtg tgatcagtg attgagaat aacttccgt gatggtggc cccggtctt ttttgccca gttgtgtgt gttgcttgt ttgcggggc ggattatgc taatgccgg caaagacgt tcccacgag tcacatcga ggtggtggc tggtgagcc tgtgtatct gccttgcga catctccac cggggaccc cagcgacca ggttttgct ggtgttgtg gtacaggga agatttagg gacccccat atacagtgt ggacggcag ggatcggga tttcagtct ggccgagcg ttggtccga tgaaaatgt gtttgccaa cagggcgta cttcatgcc cgagaagca accagctga gttaggggt ggaccacat cagggagac ggaccaggg ggtctacag atgcagagt cgattttaa agtggcgca gacgaggaa cagcaaagt caacctcac ggttatcgt gcccccgca aaaaatcgt catcaccaa cgagtcggg ggcgcagct ggagtccgt ggtggggcc ctttcccga gggcgcctc cttcacgct gcgctgtga cgtctttgg agccaagcc tctccctag tgtgacatg gttccgtaa cgatgtggt ggtgagcaa cgtcagtgt ggctttacc acgagctgg aacgacgca cgttcgtag cgagttgcg gataacagg tctgggacg tcgcgacgt ccactccga actcacctg tcaggcgtc caataatcc gaaaacacc ccctcttgc ctccaccct ccacgtaga catgaattt cggtccgct cgatgtcaa gatcctggg agccaacca gccgctttc cgccggaag gagatacga tttgctgtg tcagagttc cgggtcaag acctccggc cagcatcac ctggtggag gaacgggca gcggctcga aggggccaa aaagacgac gtccaacga cggcaacac aaccaccag cactttatc atttgtacc gagaaaaga ggatgacgg aaaatatct atcatgcag agccgaaaa caaaatgat gtctacaga cagactcga ggataaatg gaaattaga aatacacta tacccccga ggcgaagat aattctggg cacttcgct caatccgga ttccattcg ggaaggaac agacgtcta ttttgactg cgtcatcaa cgcacatcc tcccgtcta caaagtcga gtggaaaca caacggcaa ggcgctcaa tatcaacat acaagcggg gatcatcat cacaaacca aagcctggt gctgcaagg ggtctccag atcgacggc aggaaatta cacctgcgt ggggtacaa cacggaggg ggacggcga gagcaaccc cttctatct caacgtcat gtacgcgcc gacgtgcaa gccgaacca gagcagaat ttacggtgt cgcgaaaca agaacaagc acaaataac gtgtcaagt cgatgcaaa tcctcccga cgtccaatt tcgatggac gtttaataa ttctgcaga ttccgtcga cgtggccca atcgcatat tgccagaag tggaacgtc gagtgtcgt ttcttacac tccgatgac tgaactcga ttacgggac gcttttgtg ttacgcttc gaatagaat tgggacgca acgagtgcc ctgcgtgtt ccacatcat tgcagcagg acgccctga ccaagtcca caactgtac gctatccaa catctccat gacgtcctt ttccattag atgttcgga gggttttaa tggcggttt gccgcagtc ctttctatt agaggttcg cgaaagtca cagccagga ggtaaaaag caacctctc atcccctgt tccgagatt ttcagtgac aactttaga ggcgggcgc ccaatacca ggctgcctt gttctcgta caacttgaa aggacgaag tgaaccggt catcttaca agctgccac tctacgact tcctgaaaa acaactcac tgccgaaaa agacaacca ccggtccgc ctttcgttt tacgccgat gatgagtgt cctaattgg aatcgtctc agcgctttt gattgtcgg actcgtggt gattttgct ggtgcgctt gcaatgcac tcaaaacga cgacagaag gaaaagaca caaaaatgc aacggctac gggaggaaa tctcgaaca cagaggctc caccagcgg accgacgtt aagcgacaa gggaggtgg cagtccaat atcgaaaca cgattcaag cgcaggcga atgcgacag cgacgagaa gaaccccga cataattcc gcagcccgt cgacaccga cgagaccgc tgattacgt caggaaaag acaacacat tagcacaat cgaaacatc tcccagtcg gagtctttt acatcaagc cggcaattc agcaggggg cggcggggg cggcaacgc cggttacat cggttactg cacacttcg caacggaat gcccttaca cgaactcgc cgcacaatc gaaaatgca cgctgcgcc gcccccgat gatgggggg ctacgcccc ctgcacgct gccccgcca gccgcagca gtggcagta cggccccgt gagccacct gcccccggg ggtgtaccc ggtgccgta ccgcggccc ccatccgca gcagaggcc gcgtccgca gccccggga gccccccat acccatgca gcccctcgc cacgacgga agaggagcc ctcggtgga cacccccct catggtgaa caagcggga aagcacggt ttgacaagt gacttccct gtaaacgct gccaaagac agggttcca ataattgtt ttaagtatt cttatatct accaacgaa attgtacca acaggctaa attcactgg gaacaaagg gtttacttt ttttcttcc gggaattat ttacgcttg gcaaaatta acatatctt ggaaggttt acgtgctgt gagggtggg gtttcttat tttttattc gctggtgta cgtcgtcgc gagtggttg taaaaaaga taaacttat gaaaatgtt actagcttc aaggaaatc attgttgta taggccgtt tttgtcaaa tgcgttttg cctactgaa agtttttgt attcgcttc atttatacg agtgttatt tttgtatat tttgtacat aaacctagg taatgaaaa taaatgtga taattataa
