Status
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Gene information
General information
TC031184
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009567,TC009570
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2653191..2752693
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031184
    iBeetle screen iB_04657

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031184 RA
      MVAPVFFAQ LCVLLVCGA DYANAGKDV PTSHIEVVA 
      GEPVYLPCD ISTGDPSDQ VLLVLWYRE DLGTPIYSV 
      DGRDRDFSL AERWSDENV FANRAYFMP EKQPAELGV 
      DHIRETDQG VYRCRVDFK VAQTRNSKV NLTVIVPPQ 
      KIVITNESG AQLESVVGP FPEGASFTL RCDVFGAKP 
      LPSVTWFRN DVVVSNVSV ALPRAGTTH VRSELRITG 
      LGRRDVHSE LTCQASNNP KTPPLASTL HVDMNFGPL 
      DVKILGANQ PLSAGRRYD LLCQSSGSR PPASITWWR 
      NGQRLEGAK KTTSNDGNT TTSTLSFVP RKEDDGKYL 
      SCRAENKMM STDRLEDKW KLEIHYTPE AKIILGTSL 
      NPDSIREGT DVYFDCVIN AHPPVYKVE WKHNGKALN 
      INIQAGIII TNQSLVLQG VSRSTAGNY TCVGYNTEG 
      DGESNPFYL NVMYAPTCK PNQSRIYGV AKQEQAQIT 
      CQVDANPPD VQFRWTFNN SADSVDVAQ SHIARSGTS 
      SVVSYTPMT ELDYGTLLC YASNRIGTQ RVPCVFHII 
      AAGRPDQVH NCTLSNISM TSFSIRCSE GFNGGLPQS 
      FLLEVRESH SQEVKSNLS SPVPRFSVT TLEAGAQYQ 
      AALFSYNLK GRSEPVILQ AATLRLPEK QLTAEKDNH 
      RSAFRFTPM MSVLIGIVS ALLIVGLVV ILLVRLQCT 
      QNDDRRKRH KNATATGGN LEHRGSTSG PTLSDKGGG 
      SPISKHDSS AGECDSDEK NPDIIPQPV DTDETADYV 
      RKRQHISTI ETSPSRSLL HQAGNSAGG GGGGNAGYI 
      GYCTLRNGM PLHELAAQS KMHAAPPPM MGGYAPCTL 
      PRQPQQWQY GPVSHLPPG VYPVPYRGP HPQQRPRPQ 
      PREPPIPMQ PLATTEEEP SVDTPLMVN KRESTV
    CDS sequence
    • >TC031184 RA
      atggtggcc ccggtcttt tttgcccag ttgtgtgtg 
      ttgcttgtt tgcggggcg gattatgct aatgccggc 
      aaagacgtt cccacgagt cacatcgag gtggtggct 
      ggtgagcct gtgtatctg ccttgcgac atctccacc 
      ggggacccc agcgaccag gttttgctg gtgttgtgg 
      tacagggaa gatttaggg acccccata tacagtgtg 
      gacggcagg gatcgggat ttcagtctg gccgagcgt 
      tggtccgat gaaaatgtg tttgccaac agggcgtac 
      ttcatgccc gagaagcaa ccagctgag ttaggggtg 
      gaccacatc agggagacg gaccagggg gtctacaga 
      tgcagagtc gattttaaa gtggcgcag acgaggaac 
      agcaaagtc aacctcacg gttatcgtg cccccgcaa 
      aaaatcgtc atcaccaac gagtcgggg gcgcagctg 
      gagtccgtg gtggggccc tttcccgag ggcgcctcc 
      ttcacgctg cgctgtgac gtctttgga gccaagcct 
      ctccctagt gtgacatgg ttccgtaac gatgtggtg 
      gtgagcaac gtcagtgtg gctttacca cgagctgga 
      acgacgcac gttcgtagc gagttgcgg ataacaggt 
      ctgggacgt cgcgacgtc cactccgaa ctcacctgt 
      caggcgtcc aataatccg aaaacaccc cctcttgcc 
      tccaccctc cacgtagac atgaatttc ggtccgctc 
      gatgtcaag atcctggga gccaaccag ccgctttcc 
      gccggaagg agatacgat ttgctgtgt cagagttcc 
      gggtcaaga cctccggcc agcatcacc tggtggagg 
      aacgggcag cggctcgaa ggggccaaa aagacgacg 
      tccaacgac ggcaacaca accaccagc actttatca 
      tttgtaccg agaaaagag gatgacgga aaatatcta 
      tcatgcaga gccgaaaac aaaatgatg tctacagac 
      agactcgag gataaatgg aaattagaa atacactat 
      acccccgag gcgaagata attctgggc acttcgctc 
      aatccggat tccattcgg gaaggaaca gacgtctat 
      tttgactgc gtcatcaac gcacatcct cccgtctac 
      aaagtcgag tggaaacac aacggcaag gcgctcaat 
      atcaacata caagcgggg atcatcatc acaaaccaa 
      agcctggtg ctgcaaggg gtctccaga tcgacggca 
      ggaaattac acctgcgtg gggtacaac acggagggg 
      gacggcgag agcaacccc ttctatctc aacgtcatg 
      tacgcgccg acgtgcaag ccgaaccag agcagaatt 
      tacggtgtc gcgaaacaa gaacaagca caaataacg 
      tgtcaagtc gatgcaaat cctcccgac gtccaattt 
      cgatggacg tttaataat tctgcagat tccgtcgac 
      gtggcccaa tcgcatatt gccagaagt ggaacgtcg 
      agtgtcgtt tcttacact ccgatgact gaactcgat 
      tacgggacg cttttgtgt tacgcttcg aatagaatt 
      gggacgcaa cgagtgccc tgcgtgttc cacatcatt 
      gcagcagga cgccctgac caagtccac aactgtacg 
      ctatccaac atctccatg acgtccttt tccattaga 
      tgttcggag ggttttaat ggcggtttg ccgcagtcc 
      tttctatta gaggttcgc gaaagtcac agccaggag 
      gtaaaaagc aacctctca tcccctgtt ccgagattt 
      tcagtgaca actttagag gcgggcgcc caataccag 
      gctgccttg ttctcgtac aacttgaaa ggacgaagt 
      gaaccggtc atcttacaa gctgccact ctacgactt 
      cctgaaaaa caactcact gccgaaaaa gacaaccac 
      cggtccgcc tttcgtttt acgccgatg atgagtgtc 
      ctaattgga atcgtctca gcgcttttg attgtcgga 
      ctcgtggtg attttgctg gtgcgcttg caatgcact 
      caaaacgac gacagaagg aaaagacac aaaaatgca 
      acggctacg ggaggaaat ctcgaacac agaggctcc 
      accagcgga ccgacgtta agcgacaag ggaggtggc 
      agtccaata tcgaaacac gattcaagc gcaggcgaa 
      tgcgacagc gacgagaag aaccccgac ataattccg 
      cagcccgtc gacaccgac gagaccgct gattacgtc 
      aggaaaaga caacacatt agcacaatc gaaacatct 
      cccagtcgg agtctttta catcaagcc ggcaattca 
      gcagggggc ggcgggggc ggcaacgcc ggttacatc 
      ggttactgc acacttcgc aacggaatg cccttacac 
      gaactcgcc gcacaatcg aaaatgcac gctgcgccg 
      cccccgatg atggggggc tacgccccc tgcacgctg 
      ccccgccag ccgcagcag tggcagtac ggccccgtg 
      agccacctg cccccgggg gtgtacccg gtgccgtac 
      cgcggcccc catccgcag cagaggccg cgtccgcag 
      ccccgggag ccccccata cccatgcag cccctcgcc 
      acgacggaa gaggagccc tcggtggac acccccctc 
      atggtgaac aagcgggaa agcacggtt tga
    mRNA sequence
    • >TC031184 RA
      tatgacgaa agcgtcatc agcgttggc caagagatc 
      gataataaa ttagtttcg cacgatttt tccggtggc 
      gatttgtag gggaggccg ggcctaagc cgagttcgt 
      aaagtaaat gttttggta gttgggtcg gaggagttg 
      agtggtggc cccaggccc cagatgcgt ctccaagga 
      ttgcgactt ccggttatg acttgaagt gtgagtgtg 
      tgatcagtg attgagaat aacttccgt gatggtggc 
      cccggtctt ttttgccca gttgtgtgt gttgcttgt 
      ttgcggggc ggattatgc taatgccgg caaagacgt 
      tcccacgag tcacatcga ggtggtggc tggtgagcc 
      tgtgtatct gccttgcga catctccac cggggaccc 
      cagcgacca ggttttgct ggtgttgtg gtacaggga 
      agatttagg gacccccat atacagtgt ggacggcag 
      ggatcggga tttcagtct ggccgagcg ttggtccga 
      tgaaaatgt gtttgccaa cagggcgta cttcatgcc 
      cgagaagca accagctga gttaggggt ggaccacat 
      cagggagac ggaccaggg ggtctacag atgcagagt 
      cgattttaa agtggcgca gacgaggaa cagcaaagt 
      caacctcac ggttatcgt gcccccgca aaaaatcgt 
      catcaccaa cgagtcggg ggcgcagct ggagtccgt 
      ggtggggcc ctttcccga gggcgcctc cttcacgct 
      gcgctgtga cgtctttgg agccaagcc tctccctag 
      tgtgacatg gttccgtaa cgatgtggt ggtgagcaa 
      cgtcagtgt ggctttacc acgagctgg aacgacgca 
      cgttcgtag cgagttgcg gataacagg tctgggacg 
      tcgcgacgt ccactccga actcacctg tcaggcgtc 
      caataatcc gaaaacacc ccctcttgc ctccaccct 
      ccacgtaga catgaattt cggtccgct cgatgtcaa 
      gatcctggg agccaacca gccgctttc cgccggaag 
      gagatacga tttgctgtg tcagagttc cgggtcaag 
      acctccggc cagcatcac ctggtggag gaacgggca 
      gcggctcga aggggccaa aaagacgac gtccaacga 
      cggcaacac aaccaccag cactttatc atttgtacc 
      gagaaaaga ggatgacgg aaaatatct atcatgcag 
      agccgaaaa caaaatgat gtctacaga cagactcga 
      ggataaatg gaaattaga aatacacta tacccccga 
      ggcgaagat aattctggg cacttcgct caatccgga 
      ttccattcg ggaaggaac agacgtcta ttttgactg 
      cgtcatcaa cgcacatcc tcccgtcta caaagtcga 
      gtggaaaca caacggcaa ggcgctcaa tatcaacat 
      acaagcggg gatcatcat cacaaacca aagcctggt 
      gctgcaagg ggtctccag atcgacggc aggaaatta 
      cacctgcgt ggggtacaa cacggaggg ggacggcga 
      gagcaaccc cttctatct caacgtcat gtacgcgcc 
      gacgtgcaa gccgaacca gagcagaat ttacggtgt 
      cgcgaaaca agaacaagc acaaataac gtgtcaagt 
      cgatgcaaa tcctcccga cgtccaatt tcgatggac 
      gtttaataa ttctgcaga ttccgtcga cgtggccca 
      atcgcatat tgccagaag tggaacgtc gagtgtcgt 
      ttcttacac tccgatgac tgaactcga ttacgggac 
      gcttttgtg ttacgcttc gaatagaat tgggacgca 
      acgagtgcc ctgcgtgtt ccacatcat tgcagcagg 
      acgccctga ccaagtcca caactgtac gctatccaa 
      catctccat gacgtcctt ttccattag atgttcgga 
      gggttttaa tggcggttt gccgcagtc ctttctatt 
      agaggttcg cgaaagtca cagccagga ggtaaaaag 
      caacctctc atcccctgt tccgagatt ttcagtgac 
      aactttaga ggcgggcgc ccaatacca ggctgcctt 
      gttctcgta caacttgaa aggacgaag tgaaccggt 
      catcttaca agctgccac tctacgact tcctgaaaa 
      acaactcac tgccgaaaa agacaacca ccggtccgc 
      ctttcgttt tacgccgat gatgagtgt cctaattgg 
      aatcgtctc agcgctttt gattgtcgg actcgtggt 
      gattttgct ggtgcgctt gcaatgcac tcaaaacga 
      cgacagaag gaaaagaca caaaaatgc aacggctac 
      gggaggaaa tctcgaaca cagaggctc caccagcgg 
      accgacgtt aagcgacaa gggaggtgg cagtccaat 
      atcgaaaca cgattcaag cgcaggcga atgcgacag 
      cgacgagaa gaaccccga cataattcc gcagcccgt 
      cgacaccga cgagaccgc tgattacgt caggaaaag 
      acaacacat tagcacaat cgaaacatc tcccagtcg 
      gagtctttt acatcaagc cggcaattc agcaggggg 
      cggcggggg cggcaacgc cggttacat cggttactg 
      cacacttcg caacggaat gcccttaca cgaactcgc 
      cgcacaatc gaaaatgca cgctgcgcc gcccccgat 
      gatgggggg ctacgcccc ctgcacgct gccccgcca 
      gccgcagca gtggcagta cggccccgt gagccacct 
      gcccccggg ggtgtaccc ggtgccgta ccgcggccc 
      ccatccgca gcagaggcc gcgtccgca gccccggga 
      gccccccat acccatgca gcccctcgc cacgacgga 
      agaggagcc ctcggtgga cacccccct catggtgaa 
      caagcggga aagcacggt ttgacaagt gacttccct 
      gtaaacgct gccaaagac agggttcca ataattgtt 
      ttaagtatt cttatatct accaacgaa attgtacca 
      acaggctaa attcactgg gaacaaagg gtttacttt 
      ttttcttcc gggaattat ttacgcttg gcaaaatta 
      acatatctt ggaaggttt acgtgctgt gagggtggg 
      gtttcttat tttttattc gctggtgta cgtcgtcgc 
      gagtggttg taaaaaaga taaacttat gaaaatgtt 
      actagcttc aaggaaatc attgttgta taggccgtt 
      tttgtcaaa tgcgttttg cctactgaa agtttttgt 
      attcgcttc atttatacg agtgttatt tttgtatat 
      tttgtacat aaacctagg taatgaaaa taaatgtga 
      taattataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY