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Gene information
General information
TC009563 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2583274..2586643
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009563
    iBeetle screen iB_04656

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment random potentiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009563 RA
      MVSNGDNWV RIFDIPDIS KHRKGFTIY KVISMLYPE 
      SCPDAITKI TVWKRFNDF KKLHREIKV LHNKLNIKE 
      KFPSLPTST YFKRFSDET VQERRQSIL NFLEYVGYH 
      SQLFTSSEF VKFFETSHT PVDQLNGNI NAIRADLNL 
      PEDPEYCIA NSDDDRTIS DTDSVTTNS DFFADSVPQ 
      KTLSKSRLS LSHSHQSKN SDNISISTT TDSITLLDG 
      QIYPTPPNT PSCAENYAQ YIIDASIHV NIATELENE 
      KKYEDAFTA YKTAIDILL KYGKDDKNY DRRQIVRYK 
      TDKYLLRAE KIYNMHLAP EVQSLKRIT ESEENGQNA 
      DQYVQRPIS DLYKYKVVR VIASGMLVL HSELQQLFY 
      IKVIHKTMK FLNDNLILP ENVPYMVKL CNYYNCENA 
      LFLVLEYCS GKALWDYVK CRSEDPSDI FYPNEAQIL 
      RQVHDMDLS DPESEHSYS DLINDYASN KGKNSTKCD 
      DDDDDYVKV DPGFNRNLM DEPVPSKLP DLLRKSSRK 
      FSEMSSFDD LDLAQSLLI PHENIVKWA AQLLLALEK 
      VHALGVVCH DLQSRNLLI DDEGDLVLT YMCNVNDVF 
      APESLQNVA PEIFSFEPV TTAADWWCY GAILYELLV 
      GMPLSEIYP EGLTSHTVL KVPKYVSPE GRSLLRQLL 
      TFEPQKRLG FGANGPENL KSHPFFHSV DWEQLLSKY 
      NFKV
    CDS sequence
    • >TC009563 RA
      atggtgtcg aatggtgac aactgggtc cgcattttt 
      gatatccct gatatcagc aagcaccgg aagggcttt 
      accatctac aaagtcatc tcaatgctt tatcccgag 
      tcttgcccc gacgcgata acaaaaatt accgtctgg 
      aaacgtttc aatgacttt aagaagctc caccgcgaa 
      atcaaagtc ttgcacaat aaattgaac atcaaagag 
      aagttccct agtttgccc acctccacc tacttcaaa 
      aggtttagt gacgagact gtgcaggag agaagacag 
      agcattttg aattttttg gaatacgtc ggttatcac 
      tctcagttg ttcacttca agcgagttt gttaagttc 
      tttgaaacg agtcacacg cctgttgac caactgaac 
      ggtaatatt aatgcaatt agagccgat ttaaactta 
      cctgaggac cctgaatat tgcatagcc aactctgat 
      gatgatcgc acgatcagc gatactgat tcagtaaca 
      acaaatagc gattttttt gccgattct gtgcctcaa 
      aaaaccctc tctaaatcc cgtttaagc ctcagccac 
      tcgcaccaa tccaaaaat tctgataac ataagtata 
      tccacgacc actgacagt ataacacta ttggacggt 
      cagatttat ccaacgcct cccaatact cccagttgt 
      gccgagaat tatgcacaa tatatcata gacgcttca 
      attcacgta aatattgcc acggaatta gaaaacgaa 
      aagaaatat gaggacgct ttcacggct tataaaacc 
      gcaattgat attttgctg aaatatggg aaagacgat 
      aaaaattac gataggaga caaatagtg aggtacaaa 
      actgataaa tatttactg agggccgaa aaaatttat 
      aacatgcat ttggccccc gaagtgcaa agtctgaag 
      cgaattacc gaatcggag gaaaacggc caaaacgcc 
      gatcaatac gtgcaacga cccatatcc gacttatac 
      aaatataaa gtggtccgt gttatagcc tccggaatg 
      ttggttttg cattccgag ctccagcaa ctcttttat 
      attaaagtt atacacaag actatgaaa tttctgaac 
      gacaacttg attttgccc gagaacgtg ccttacatg 
      gttaagttg tgcaattat tacaattgc gaaaacgcc 
      ttgttttta gtgttagag tactgtagc ggtaaagca 
      ctatgggat tacgtgaag tgcaggtct gaggaccct 
      tctgatatt ttttacccg aatgaggcg caaatcctg 
      cgccaagtg cacgatatg gatctttcc gatccggaa 
      tcggagcat agttacagc gatttaatc aacgattac 
      gcaagcaac aaaggcaaa aactcgaca aaatgcgac 
      gacgacgac gatgattac gtgaaagtc gatccgggc 
      tttaataga aacttaatg gacgaacct gtcccgtcc 
      aaactcccc gatttatta agaaaatca tcacggaag 
      ttcagcgaa atgtcgagc ttcgacgac ttggatctg 
      gcacaaagt ctcctcatt ccacacgag aacatcgtc 
      aagtgggcg gcgcaactc ctactagcg ttggagaaa 
      gtgcacgcc ttgggagtt gtgtgccac gacttgcag 
      tctcgcaat ttgttaatt gacgacgag ggcgacctg 
      gtgctcacc tacatgtgt aacgtgaac gacgttttt 
      gccccagaa agtttacaa aacgtagca ccggaaatt 
      ttttctttc gaaccggtt acaaccgcc gccgattgg 
      tggtgttat ggcgctatt ttgtacgaa cttttggtc 
      ggaatgcct ttgtcagaa atttatcca gaagggtta 
      acgagtcat acagttttg aaagttccc aaatatgtg 
      tcaccagaa gggcggtct ttgttgagg caacttttg 
      acgtttgag ccgcaaaaa cggctaggt tttggggcc 
      aatggtcca gaaaattta aaatcgcat ccgtttttt 
      cattcagtt gattgggaa caattgctt tcaaagtac 
      aattttaaa gtctga
    mRNA sequence
    • >TC009563 RA
      tgtcacttt gacagccgc cttatcgct agcaaaaaa 
      tgtgaaatt ttatcaaaa agtcaaaaa atggtgtcg 
      aatggtgac aactgggtc cgcattttt gatatccct 
      gatatcagc aagcaccgg aagggcttt accatctac 
      aaagtcatc tcaatgctt tatcccgag tcttgcccc 
      gacgcgata acaaaaatt accgtctgg aaacgtttc 
      aatgacttt aagaagctc caccgcgaa atcaaagtc 
      ttgcacaat aaattgaac atcaaagag aagttccct 
      agtttgccc acctccacc tacttcaaa aggtttagt 
      gacgagact gtgcaggag agaagacag agcattttg 
      aattttttg gaatacgtc ggttatcac tctcagttg 
      ttcacttca agcgagttt gttaagttc tttgaaacg 
      agtcacacg cctgttgac caactgaac ggtaatatt 
      aatgcaatt agagccgat ttaaactta cctgaggac 
      cctgaatat tgcatagcc aactctgat gatgatcgc 
      acgatcagc gatactgat tcagtaaca acaaatagc 
      gattttttt gccgattct gtgcctcaa aaaaccctc 
      tctaaatcc cgtttaagc ctcagccac tcgcaccaa 
      tccaaaaat tctgataac ataagtata tccacgacc 
      actgacagt ataacacta ttggacggt cagatttat 
      ccaacgcct cccaatact cccagttgt gccgagaat 
      tatgcacaa tatatcata gacgcttca attcacgta 
      aatattgcc acggaatta gaaaacgaa aagaaatat 
      gaggacgct ttcacggct tataaaacc gcaattgat 
      attttgctg aaatatggg aaagacgat aaaaattac 
      gataggaga caaatagtg aggtacaaa actgataaa 
      tatttactg agggccgaa aaaatttat aacatgcat 
      ttggccccc gaagtgcaa agtctgaag cgaattacc 
      gaatcggag gaaaacggc caaaacgcc gatcaatac 
      gtgcaacga cccatatcc gacttatac aaatataaa 
      gtggtccgt gttatagcc tccggaatg ttggttttg 
      cattccgag ctccagcaa ctcttttat attaaagtt 
      atacacaag actatgaaa tttctgaac gacaacttg 
      attttgccc gagaacgtg ccttacatg gttaagttg 
      tgcaattat tacaattgc gaaaacgcc ttgttttta 
      gtgttagag tactgtagc ggtaaagca ctatgggat 
      tacgtgaag tgcaggtct gaggaccct tctgatatt 
      ttttacccg aatgaggcg caaatcctg cgccaagtg 
      cacgatatg gatctttcc gatccggaa tcggagcat 
      agttacagc gatttaatc aacgattac gcaagcaac 
      aaaggcaaa aactcgaca aaatgcgac gacgacgac 
      gatgattac gtgaaagtc gatccgggc tttaataga 
      aacttaatg gacgaacct gtcccgtcc aaactcccc 
      gatttatta agaaaatca tcacggaag ttcagcgaa 
      atgtcgagc ttcgacgac ttggatctg gcacaaagt 
      ctcctcatt ccacacgag aacatcgtc aagtgggcg 
      gcgcaactc ctactagcg ttggagaaa gtgcacgcc 
      ttgggagtt gtgtgccac gacttgcag tctcgcaat 
      ttgttaatt gacgacgag ggcgacctg gtgctcacc 
      tacatgtgt aacgtgaac gacgttttt gccccagaa 
      agtttacaa aacgtagca ccggaaatt ttttctttc 
      gaaccggtt acaaccgcc gccgattgg tggtgttat 
      ggcgctatt ttgtacgaa cttttggtc ggaatgcct 
      ttgtcagaa atttatcca gaagggtta acgagtcat 
      acagttttg aaagttccc aaatatgtg tcaccagaa 
      gggcggtct ttgttgagg caacttttg acgtttgag 
      ccgcaaaaa cggctaggt tttggggcc aatggtcca 
      gaaaattta aaatcgcat ccgtttttt cattcagtt 
      gattgggaa caattgctt tcaaagtac aattttaaa 
      gtctgattt tgttaagtg ttcagtatt taggtgcgt 
      tgcttatta cattccacg tgttttgtt gcttttgta 
      ctaaataaa gttttgtta tctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY