Status
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Gene information
General information
TC009552 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2360268..2365314
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009552
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04655

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009552 RA
      MKSYPYVPS MVHAITVLT VLKMMTKAQ TSCGSGSMN 
      GRVMYERLP NIQLQGFDD DVVRDTAPP FKVLEKCEQ 
      LCLTDRASN NIVRTCTSF DFQPGSRIA TYNGGPEYE 
      ESTCYLTAE QARPEGIGS LMLVPNSVH FTEVCLTSN 
      RIDRDCPNR LYIFERHPR KKLKLPDAD LKEVMATNR 
      SECEDKCLN EFSFVCRSA TFDPAMRTC TLSRFTRRT 
      HPELLKDDP NSDYLENTC LNADRRCDG LAVFIKEEN 
      KRLGGPFEV DLFTNLTLE ECQSMCVHA EKYFCRSIE 
      YDEMTKVCT LSEEDSISQ KEEIGLASS PTHNFYDFA 
      CLDSPRGSE YPDNHATSH LFSGKKPDT AFQRYRNSR 
      LGGEHHSEI SGRSLSECL DECLRQPSF QCKSAEYSE 
      RHRICRLTK FSQKDGMRI IYDADYDYY ENLMLGGDE 
      GQRPGDGYP GGGGDRYRP GGGRYPPVG GGRYPPSRY 
      PPSSGGGDG YDRPGRPPY DRYPDDRYR PPGGDLYPS 
      GDDRYPPPR PPVDSYPDS GTRYPIGPP GGDRYPGRY 
      PPGDRDRYP YYPPSSSGG YPSRYPSRY PDKYGDRYG 
      DRYPDKYGD RYPDKYGDR YPDKYDRYP DKYGDRYGD 
      RYGGLNGRY GESDYYYGG SKYGGGGRY GGDRYWSGP 
      KYGGKYDDR YGNRHSYYG DERDGGNRW FFRPDRRPG 
      RPVDGGRPI YEPRPPYDG RPYEERPNR RPISPGPDG 
      PIYDTFGGI GPNRPYSSR CDEGDSFKQ VGSRQRLRR 
      EFVRKFMNV PSLSQCQRE CTEMRDFIC RSFNYRDVI 
      GREDDRDNC ELSDRDSRD LEANSRRFF DNTGNYDFY 
      ERSISRQGV DGDCLDVSQ VCNEDGMEF TLRTPEGFY 
      GRIYTYGFY DRCFFRGNG GTVNVLRIS GAQGYPECG 
      TQRYGDTMT NIVVVQFSD FVQTGRDKR FNLTCLFRG 
      PGEAVVSSG FIGAGSGSP IPIEYLPAE NTLSSKVRL 
      MILYQGRPT TTIAVGDPL TFRLEAQDG YNYITDIFA 
      TNVVARDPY SGRSVQLID RFGCPVDSF VFPELDRGR 
      NGDSLEARF NAFKIPESN FLVFEATVR TCREGCQPA 
      YCQTGAGRS EPSFGRKRR SINETLLLE SNNTISAED 
      DEITNTTAT NDTTVSFLE TTKIDTIDD ASEEKESES 
      PEFVREMIE VFDSREELQ QEARRADPA PETVCLTPG 
      EYHGLVSAV FALIAILLT ISLVSGLAY RKYWIVMKK 
      NILADRASS NISSSYPNT RSSGLSSGI SIFGTGLQK 
      QFIGFGRSR NFPSLPKDD DIECPGPAP GGPFEDPSE 
      PIYTDPSLF ERSRSLRSI AVSQKRRKS PGQQI
    CDS sequence
    • >TC009552 RA
      atgaaaagt tatccgtac gttcccagc atggtgcac 
      gcgattaca gttctcacg gttttgaaa atgatgacg 
      aaagcccaa acatcttgc ggctctgga tcaatgaat 
      ggaagagta atgtatgaa agactcccg aatatacaa 
      ctgcaagga ttcgacgat gacgtggtt agagatacg 
      gcaccgccg ttcaaggtt ttagaaaaa tgcgaacaa 
      ctctgcctc actgacagg gcctcgaat aacatcgtc 
      cggacctgt acttcgttt gatttccaa ccaggaagt 
      cggatagcc acctataat ggaggccca gaatacgag 
      gaatcaacg tgttatctc acagcagaa caagcgaga 
      cctgaaggg attgggagt ttgatgttg gtccccaac 
      agtgtccac ttcactgaa gtttgtcta acttccaac 
      agaatcgac agagattgc cccaacaga ctctacatc 
      tttgaaaga cacccgcgc aaaaaacta aaactcccg 
      gacgcagat cttaaggaa gtaatggca acgaatcgg 
      agcgaatgc gaggacaaa tgcctgaac gaattcagt 
      tttgtttgc cgttctgcc acgttcgat ccagcaatg 
      cgaacgtgt actttaagc cgattcact cgacgaact 
      catccagaa ttgttaaaa gacgacccc aactcggat 
      tatttagaa aacacttgt ctcaatgcc gaccgaaga 
      tgcgacggt ctggccgtc ttcatcaaa gaagaaaac 
      aagcgtcta ggcggcccc ttcgaagtc gatctcttc 
      actaacctg actttagaa gaatgccag tcgatgtgc 
      gtccacgcc gagaaatac ttctgccgc tcgatcgaa 
      tacgacgaa atgaccaaa gtgtgcacc ctatcagag 
      gaagactct atctcacag aaagaagaa atcgggctt 
      gccagcagc cccactcac aacttctac gattttgcg 
      tgtttagac agtccccgc ggttccgaa tatcccgac 
      aatcacgcc acttcgcat ctcttcagt ggcaaaaaa 
      cccgacact gcgttccaa aggtacagg aacagtcga 
      cttggcggc gaacaccat tcggaaatt tctggacgt 
      tctttgagc gaatgcctg gacgagtgt ttacgccaa 
      cctagcttc cagtgcaag tcggcggaa tacagcgaa 
      aggcatcga atttgccgc ttgacgaaa ttcagccaa 
      aaggacggg atgaggatt atttacgac gccgattac 
      gactattac gaaaacttg atgcttgga ggagatgag 
      ggccagaga cctggtgat gggtatccg ggaggaggc 
      ggtgatcgg taccggccg ggtggtggt cgctaccct 
      ccggtaggt gggggacgg taccctcca agtcgctat 
      ccgccaagt agtggaggt ggtgatggg tatgatcgc 
      ccaggaaga ccaccatat gatcggtac ccggatgat 
      aggtaccga ccaccagga ggcgatttg tatccttcg 
      ggagacgat aggtatcct ccgccacga ccacctgtg 
      gatagttat ccagattca gggacgcga tacccgatc 
      gggcctcca ggtggtgat agatatccc gggagatat 
      cctcccgga gatcgggat agatatcca tactatcct 
      ccatcgtca agtggagga tatcctagt cggtacccg 
      agccgatat cccgacaaa tatggggat agatatgga 
      gacaggtac ccggacaaa tacggagat agatatccg 
      gacaaatac ggtgacaga tatccggac aaatacgac 
      agatatcca gacaaatat ggagacaga tatggtgat 
      cggtatgga ggtctgaac ggtagatac ggcgaatca 
      gattattac tacggaggt tcgaaatat ggaggaggc 
      ggccgttat gggggagac agatactgg agtggtcca 
      aaatacggt ggcaaatac gacgaccgg tacggtaac 
      cgacacagc tactacggg gacgaacga gatggagga 
      aaccgatgg tttttccga ccagaccgc agacctggt 
      cgcccagtt gatggtggg agacccatc tacgaacca 
      agaccgccg tacgacggc cgtccgtac gaagaaaga 
      cccaacaga cgtccaatt tcgccaggt cctgacgga 
      ccaatctat gacaccttt ggcggaatc ggtccgaac 
      agaccttac agttccagg tgtgacgaa ggtgatagt 
      tttaaacaa gtcgggagc cggcaacga cttcggcgg 
      gaatttgtt agaaaattc atgaacgtt ccgtcactg 
      agtcaatgt cagcgtgaa tgtacggaa atgagggat 
      ttcatctgc cgatcgttc aactacagg gatgttata 
      ggacgtgag gacgatcga gacaactgc gagctgagc 
      gatcgcgat tcccgggat ttggaagca aacagtcgg 
      aggttcttc gacaatacg ggaaactac gacttttac 
      gaacgttcg attagtcgg cagggcgta gacggcgat 
      tgcctcgac gtaagtcaa gtgtgtaac gaagacggg 
      atggaattt acccttcga acacctgaa ggtttctac 
      ggaaggata tacacctac ggtttttac gaccgatgt 
      ttcttccgt ggaaacggc ggtactgtt aacgtgtta 
      cgaatcagt ggtgcacaa ggctatcca gaatgtggt 
      acccagcgc tatggagac accatgacc aatatcgtg 
      gtggtgcag ttcagtgat tttgtccaa actggacga 
      gacaaacga ttcaatttg acttgtctg ttccgagga 
      ccgggcgaa gctgtcgtt tcgtctgga tttatagga 
      gcgggatct ggaagtcca atcccgatc gagtatctc 
      cccgctgaa aacaccctc agctccaaa gtgcgtcta 
      atgatcttg taccaagga agaccaaca actacgatc 
      gccgttggt gatcctctc accttcagg ctggaagcc 
      caagacggt tacaattac atcacggat attttcgcc 
      acaaatgtg gtagctcgg gacccctat tctggacga 
      tcagtccaa ctaattgac cgatttgga tgtccagtg 
      gacagtttc gtgttccct gaattggac cgtggccga 
      aatggtgac agtcttgaa gcccgtttt aacgccttc 
      aaaatccca gaatcgaat ttcctcgtg tttgaagca 
      acggtacgc acatgtcgc gaaggttgt caacctgcc 
      tattgtcaa acaggagct ggacgatcc gaaccatca 
      tttggaaga aaacgaaga tcaattaac gaaacgctt 
      ctactagaa agcaacaac accatttca gccgaagac 
      gatgaaata accaacaca actgccacc aacgacacg 
      actgtttcg ttcctcgaa accaccaaa attgacact 
      attgacgac gcctccgaa gagaaagaa tccgaaagt 
      ccggagttt gtccgcgag atgattgaa gttttcgat 
      tcacgtgaa gagttgcaa caagaggca cgtcgtgcg 
      gacccggcc ccagaaacc gtctgcttg actcctggt 
      gaataccac gggctagtc agcgctgtt ttcgccctc 
      attgctatc cttctgacc atatcgcta gtttcgggc 
      ctcgcttat cgcaaatat tggatcgtt atgaagaag 
      aatattctc gcagatcgc gcttcgtcc aatatctcg 
      tcctcatac cccaacacc cgcagcagc ggtctctcg 
      agcgggatt tccattttt ggcacaggt ctccagaag 
      cagttcatc ggttttggg cgttctcgg aatttcccg 
      agcttgccg aaagacgac gatattgag tgtcctgga 
      cctgcacct gggggtccc ttcgaggac cccagcgaa 
      ccgatctac acggaccct tctcttttc gaacgatcg 
      cggagtctc aggagtatc gcggtgtcg caaaaacgg 
      cggaaatct cccggacag cagatttaa
    mRNA sequence
    • >TC009552 RA
      gttcgcaga aagaaaagt atgtccaga tagtcaaca 
      ccgaaaggc catagaaga tcaaaatga aaagttatc 
      cgtacgttc ccagcatgg tgcacgcga ttacagttc 
      tcacggttt tgaaaatga tgacgaaag cccaaacat 
      cttgcggct ctggatcaa tgaatggaa gagtaatgt 
      atgaaagac tcccgaata tacaactgc aaggattcg 
      acgatgacg tggttagag atacggcac cgccgttca 
      aggttttag aaaaatgcg aacaactct gcctcactg 
      acagggcct cgaataaca tcgtccgga cctgtactt 
      cgtttgatt tccaaccag gaagtcgga tagccacct 
      ataatggag gcccagaat acgaggaat caacgtgtt 
      atctcacag cagaacaag cgagacctg aagggattg 
      ggagtttga tgttggtcc ccaacagtg tccacttca 
      ctgaagttt gtctaactt ccaacagaa tcgacagag 
      attgcccca acagactct acatctttg aaagacacc 
      cgcgcaaaa aactaaaac tcccggacg cagatctta 
      aggaagtaa tggcaacga atcggagcg aatgcgagg 
      acaaatgcc tgaacgaat tcagttttg tttgccgtt 
      ctgccacgt tcgatccag caatgcgaa cgtgtactt 
      taagccgat tcactcgac gaactcatc cagaattgt 
      taaaagacg accccaact cggattatt tagaaaaca 
      cttgtctca atgccgacc gaagatgcg acggtctgg 
      ccgtcttca tcaaagaag aaaacaagc gtctaggcg 
      gccccttcg aagtcgatc tcttcacta acctgactt 
      tagaagaat gccagtcga tgtgcgtcc acgccgaga 
      aatacttct gccgctcga tcgaatacg acgaaatga 
      ccaaagtgt gcaccctat cagaggaag actctatct 
      cacagaaag aagaaatcg ggcttgcca gcagcccca 
      ctcacaact tctacgatt ttgcgtgtt tagacagtc 
      cccgcggtt ccgaatatc ccgacaatc acgccactt 
      cgcatctct tcagtggca aaaaacccg acactgcgt 
      tccaaaggt acaggaaca gtcgacttg gcggcgaac 
      accattcgg aaatttctg gacgttctt tgagcgaat 
      gcctggacg agtgtttac gccaaccta gcttccagt 
      gcaagtcgg cggaataca gcgaaaggc atcgaattt 
      gccgcttga cgaaattca gccaaaagg acgggatga 
      ggattattt acgacgccg attacgact attacgaaa 
      acttgatgc ttggaggag atgagggcc agagacctg 
      gtgatgggt atccgggag gaggcggtg atcggtacc 
      ggccgggtg gtggtcgct accctccgg taggtgggg 
      gacggtacc ctccaagtc gctatccgc caagtagtg 
      gaggtggtg atgggtatg atcgcccag gaagaccac 
      catatgatc ggtacccgg atgataggt accgaccac 
      caggaggcg atttgtatc cttcgggag acgataggt 
      atcctccgc cacgaccac ctgtggata gttatccag 
      attcaggga cgcgatacc cgatcgggc ctccaggtg 
      gtgatagat atcccggga gatatcctc ccggagatc 
      gggatagat atccatact atcctccat cgtcaagtg 
      gaggatatc ctagtcggt acccgagcc gatatcccg 
      acaaatatg gggatagat atggagaca ggtacccgg 
      acaaatacg gagatagat atccggaca aatacggtg 
      acagatatc cggacaaat acgacagat atccagaca 
      aatatggag acagatatg gtgatcggt atggaggtc 
      tgaacggta gatacggcg aatcagatt attactacg 
      gaggttcga aatatggag gaggcggcc gttatgggg 
      gagacagat actggagtg gtccaaaat acggtggca 
      aatacgacg accggtacg gtaaccgac acagctact 
      acggggacg aacgagatg gaggaaacc gatggtttt 
      tccgaccag accgcagac ctggtcgcc cagttgatg 
      gtgggagac ccatctacg aaccaagac cgccgtacg 
      acggccgtc cgtacgaag aaagaccca acagacgtc 
      caatttcgc caggtcctg acggaccaa tctatgaca 
      cctttggcg gaatcggtc cgaacagac cttacagtt 
      ccaggtgtg acgaaggtg atagtttta aacaagtcg 
      ggagccggc aacgacttc ggcgggaat ttgttagaa 
      aattcatga acgttccgt cactgagtc aatgtcagc 
      gtgaatgta cggaaatga gggatttca tctgccgat 
      cgttcaact acagggatg ttataggac gtgaggacg 
      atcgagaca actgcgagc tgagcgatc gcgattccc 
      gggatttgg aagcaaaca gtcggaggt tcttcgaca 
      atacgggaa actacgact tttacgaac gttcgatta 
      gtcggcagg gcgtagacg gcgattgcc tcgacgtaa 
      gtcaagtgt gtaacgaag acgggatgg aatttaccc 
      ttcgaacac ctgaaggtt tctacggaa ggatataca 
      cctacggtt tttacgacc gatgtttct tccgtggaa 
      acggcggta ctgttaacg tgttacgaa tcagtggtg 
      cacaaggct atccagaat gtggtaccc agcgctatg 
      gagacacca tgaccaata tcgtggtgg tgcagttca 
      gtgattttg tccaaactg gacgagaca aacgattca 
      atttgactt gtctgttcc gaggaccgg gcgaagctg 
      tcgtttcgt ctggattta taggagcgg gatctggaa 
      gtccaatcc cgatcgagt atctccccg ctgaaaaca 
      ccctcagct ccaaagtgc gtctaatga tcttgtacc 
      aaggaagac caacaacta cgatcgccg ttggtgatc 
      ctctcacct tcaggctgg aagcccaag acggttaca 
      attacatca cggatattt tcgccacaa atgtggtag 
      ctcgggacc cctattctg gacgatcag tccaactaa 
      ttgaccgat ttggatgtc cagtggaca gtttcgtgt 
      tccctgaat tggaccgtg gccgaaatg gtgacagtc 
      ttgaagccc gttttaacg ccttcaaaa tcccagaat 
      cgaatttcc tcgtgtttg aagcaacgg tacgcacat 
      gtcgcgaag gttgtcaac ctgcctatt gtcaaacag 
      gagctggac gatccgaac catcatttg gaagaaaac 
      gaagatcaa ttaacgaaa cgcttctac tagaaagca 
      acaacacca tttcagccg aagacgatg aaataacca 
      acacaactg ccaccaacg acacgactg tttcgttcc 
      tcgaaacca ccaaaattg acactattg acgacgcct 
      ccgaagaga aagaatccg aaagtccgg agtttgtcc 
      gcgagatga ttgaagttt tcgattcac gtgaagagt 
      tgcaacaag aggcacgtc gtgcggacc cggccccag 
      aaaccgtct gcttgactc ctggtgaat accacgggc 
      tagtcagcg ctgttttcg ccctcattg ctatccttc 
      tgaccatat cgctagttt cgggcctcg cttatcgca 
      aatattgga tcgttatga agaagaata ttctcgcag 
      atcgcgctt cgtccaata tctcgtcct catacccca 
      acacccgca gcagcggtc tctcgagcg ggatttcca 
      tttttggca caggtctcc agaagcagt tcatcggtt 
      ttgggcgtt ctcggaatt tcccgagct tgccgaaag 
      acgacgata ttgagtgtc ctggacctg cacctgggg 
      gtcccttcg aggacccca gcgaaccga tctacacgg 
      acccttctc ttttcgaac gatcgcgga gtctcagga 
      gtatcgcgg tgtcgcaaa aacggcgga aatctcccg 
      gacagcaga tttaatagg ttttaatgc tagagaatg 
      tgttcgcgg tccccacgt tctgacact aactttagg 
      ataaggtaa ttttgtatt tattgaaga aaaaaaggc 
      acgaggcgt cgtgggtat tgcgaacac cccgattga 
      gttttctaa tttaaatgt aggtttatt taattttag 
      gaccaggtt cattgtgca ttattttaa aatactcgt 
      gttgcaata aattcactg ccgacaacc agctccaaa 
      tgccgctca tgtcaaact attgtgatc gcagcgtga 
      gtgtagttt gactacaca aatttgcgc gattttttt 
      gtacctttt atttttatc taaacctgc ctatggccg 
      atgccatat gaaggccga ctttactgt ttctcaggc 
      acatacgca cttttcttt agttaagtt cgtccaatt 
      caacactcg ctacgagca cttctgttg ttaaacaac 
      aagttaggt ttaaaatta ataaattag gttctttat 
      a
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