| General information | |||
| TC009542 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2230877..2238347 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009542 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04654 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC009542 RA
MENSAASYL HKKFKKQLS IDPSKQHHH QNESKGVVD TRQEKTPEP NQTCKDNNE SVPNQSTNQ SSSLSKSSI SVEQCCSVI LQSSNSCIS KTWTEPVST SSILDNLYK VKPEDVASK DHEEAEKTP DKSSAGKYV CPYCNLVCS KPSVLQKHI RAHTNERPF PCNSCGFSF KTRSNLYKH CRSRTHANR VMGNKAQEM GEADEGPKS PQCSDGTIE DRAQDLKTK PYKPRFHTK HIFKENEEN KDEPKHNSN ILSDHINEI INKNNSIVN SHDPYLLKK RPEVEDHHQ RHITDLIYL NNNDEPLNL TKNRKRCMS EVVEPGTQK SLIKELLLK NLNSDMQCP HCKMIFQTV TELELHKLR SCKGFVKPG AKYSRSSSV NVASILTQN KNAFDNIPH LQNAVFPLK SPGPFLGKT RLVESDKNK SFSFDDGLP TAFPPSTPT ERYLLSPLT LPSEREKKT PVKLFGGEV KITHTSGET KSFKIDNKD DKFDSGFVE YSGKVSENR VVKSGLQSG GTVLTNKAN YPKEDSLRT NPDVIRVYD NTPASPNIG VNLTKPQIT YRGSTDPII EYAVPPYSK YTNIVDFSQ KAVKLLTPN LKQPNLAVP GVPTPNHFT FPSFTEEKI IDLEKPEPQ QKVFQPKII TPQPVCNPV KLLVNGKVV RHVPGIPGP VVPEDQPPP RLKPEPKPL EVKIDHPAD KSPVKITET KKFARPNSL ALKPTTASL KQHHGLTPT LFNQILISP DTPRVAKKY VHQFLHGNY FSYLGLKCS TRTVYCTLN KTQPFYVQH FKKLSMYSE WRQQDTKTE KLYVSAYDS RQRHQRYTT AGKTTADLV HSSYKFVVS ESSSGGDKS EEAHKSATI LGGYECNED YTYIRGRGR GRYVCDQCG IRCKKPSML KKHIRTHSN DRPYTCNHC NFSFKTKGN LTKHMKSKA HTKNYAASG SGSSTQQSG TQSSESDTE DSGMDSSDE STRREEHEA AYGLLSLSQ KTSQSLRRE STSPMSTSG SDPQVLNTE EIAHASKTN YAKNKFLDD KTPLNFSVP KVTTTTISS TTTSSDEEV QNLTQFLGK TTINRPLTY PYTSILPPE CTSQEVAEN PETKSIKQF HVIQKYASR ESPTVDSSA NCLTSSKSA KDRTRVPSD HKVNNVPVL KVNDAVVLG DSVTDLRKR KLSYAESEF ERVKQHKTD EVMDLSMPV EKNPELRYN GSPQPYHHN LIKKNEDLY PSSKFVDEK LPVKNNGFV SVNINYVIP DSQPINCES PKIVYKTVD YDNSAMETL ADIATKQVK LEKNTLAKS VATEFLKIA TKNEFPSGD ATREANTFG PVTKEVNDL IVKPEGNKS CHICAKSFS KTPQLRLHM NIHYLERPF RCHSCSVSF RTKGHLQKH ERSASHHNK LSSSPALSS SEPRPFKCV DCNIAFRIH GHLAKHLRS KMHIMKLEC LAKIPFGLY AELERSNSL LTEINTADG DQCLESLKT LAKKVFIND PNKLNQLNS AESIDADS
- >TC009542 RA
atggaaaac agcgccgcc agttatttg cacaagaag ttcaagaag cagctgagc atcgatccg tcgaaacaa caccaccac cagaatgag agcaaggga gtcgtggac acgcgtcaa gaaaaaacc cccgaacct aatcaaact tgcaaagac aataacgaa agtgtgcca aaccagagt acaaatcaa agcagttct ctctcaaag tcttccata tcagtagag cagtgttgt agcgtgatt ttacaatct tcgaactcg tgcatatcg aaaacctgg accgaacca gtcagcacg tcttctatt ttagataac ttatacaaa gtcaagccc gaagatgta gcttcgaag gaccacgaa gaagccgag aaaaccccc gacaagtcc tcggccggc aaatatgtg tgcccctac tgcaacctg gtgtgctcc aagcccagt gtgttgcag aaacacatc agggcgcac accaacgaa cgcccgttc ccgtgcaac agttgcggg ttcagcttc aagacacgc tcgaatctc tacaaacat tgccggtcg aggacgcac gctaaccgc gtcatgggc aacaaggcg caagagatg ggagaggcg gacgaaggg cccaaaagc ccccagtgc agtgacggc accatcgag gacagagcg caagacctc aagaccaaa ccctacaaa ccacgcttc cacaccaag cacatcttc aaagagaac gaagaaaac aaagacgaa cccaaacac aactccaac atcctctcg gaccacatc aacgaaatc atcaacaag aacaactcg attgtcaac tcgcacgat ccctacttg ctgaagaag aggccagag gtggaggac caccaccag agacacatc accgacttg atttatttg aacaacaac gacgaaccc ttaaacctt acgaaaaac cgcaagcgg tgcatgagc gaagttgtg gaacccgga actcagaag agtttgatc aaagagttg ctcctcaaa aatctaaat tcggatatg caatgccca cattgcaaa atgattttt caaactgtc actgagttg gagttgcac aagctgagg agttgcaaa ggtttcgtc aaacctggt gccaaatac agtcggagt agttcagtc aatgttgcg tcgattctt acgcagaac aagaacgcc tttgacaat ataccgcac ctacagaac gcggtgttt cccctaaaa tcaccgggt cccttcttg ggcaagacc cgattggtc gaaagtgac aaaaacaaa tcgttttca ttcgacgat ggccttccg acagctttc ccaccttcg acacccact gaacgctat ttactctcg cccttgact ttaccgagc gaacgcgaa aaaaagact ccagtgaag ttgttcggt ggtgaagtc aaaatcacg cacacgtcc ggcgaaacg aagagtttc aaaattgat aacaaggac gataagttt gacagtggc tttgtggag tacagtggc aaagtgagt gagaatcgg gtggtgaag tccgggtta cagtcaggt ggtaccgtt ctaaccaac aaagctaac taccccaag gaggattct ttacggaca aatcccgac gttattagg gtgtacgac aacactcca gcctcgcct aacatcgga gtcaaccta accaaaccc caaatcacc tacagggga agtaccgac ccaataata gagtacgcc gtcccgccc tatagcaaa tacacaaac atcgtggat tttagccaa aaagccgta aaactccta acaccgaat ttaaaacaa cccaatctc gcggtcccc ggcgtgcca acccccaac catttcact ttcccaagt ttcactgaa gagaaaata atcgatttg gaaaaaccc gagccgcaa caaaaagtc ttccaaccc aaaatcata actccacaa ccggtctgt aaccccgtc aaactccta gtcaatgga aaagtcgtc cgtcatgtc cctgggatt cccggcccg gtcgtaccc gaagaccaa cccccaccc cggctcaag cccgagccg aaaccgctc gaagtgaaa atcgaccat ccggccgat aaatccccg gttaaaata accgaaacg aaaaaattc gcaaggcct aatagtctc gccttgaaa ccgacaacg gcctctttg aaacaacac cacggtcta acaccgacg ctatttaac cagattttg atcagtcct gacacacct cgtgtggcg aagaaatac gtccatcag tttttgcac gggaattat ttcagttat ttgggtttg aagtgttcg acacggacg gtgtattgt acgctgaat aagactcag ccgttttat gtgcaacat tttaaaaaa ttgagcatg tactccgag tggcggcag caggacacc aagacggag aagttgtac gtgagtgcg tacgattcg aggcagagg catcagagg tacaccacg gcggggaag accacagcg gatttggtg cattcgagt tataagttt gtcgtttcg gagtcttcg agtggcggt gataagtcc gaagaggcg cacaaatcg gccacaatt ctgggcggt tacgaatgc aatgaagac tacacttat atccgaggc agaggccga gggcgttac gtttgtgac cagtgcggg atcaggtgc aaaaaaccg tcaatgttg aagaaacat atcaggacg cattcgaat gacaggccg tatacttgt aatcattgt aattttagt ttcaagaca aaggggaat ttgacgaaa catatgaag agtaaagca cacacaaag aattatgcg gctagtgga tcaggatcg tcgacacaa cagagtggt acacaaagt tctgagtcg gatacggag gacagcggg atggatagt agtgatgaa tcaacacgt cgagaggag catgaagcg gcctacggc ctcctttcc ctctcgcag aaaacctcg cagagcctt cgtcgggag tccacaagt ccaatgtcc acatcgggc tccgacccc caagtttta aacacggaa gaaatagct cacgcttcc aagaccaac tacgcgaaa aacaaattt ctcgatgat aaaacaccg ctcaatttc agcgtccct aaagtcacc actaccacc atcagtagc accaccaca tcatcggac gaagaagtc caaaacttg acccagttc ctaggcaag acgacaatc aatcgacca ctgacttac ccctacact tcgatttta cccccggag tgtaccagt caagaggtg gcggaaaat ccggaaacg aaaagtatc aaacagttc cacgtgata caaaagtac gcctcgagg gaatccccg actgtggac agtagtgcc aattgtttg actagtagt aaaagtgca aaggatagg acacgagtg ccaagtgat cataaagtg aacaatgta ccggttcta aaagtcaac gatgctgtg gtgctcggt gatagtgtg actgatttg aggaaacga aaattgagt tatgcggaa agcgagttc gagagagtg aaacaacac aaaacggac gaagtgatg gacttgtca atgccagtc gagaaaaac cccgaattg aggtacaat ggatcgcct caaccgtac caccataac ctgattaaa aaaaacgaa gatctctac ccgtcatcg aagttcgtc gacgagaaa ttgccggtg aaaaacaac ggtttcgtg agtgtgaac atcaactac gtgataccg gacagtcaa ccgataaac tgtgaatcc cccaaaata gtgtacaaa acggtcgat tatgataac agtgcaatg gaaacgttg gctgacatt gccaccaaa caagtgaaa ctcgagaaa aatactctt gcgaaaagt gtggcgacg gagtttttg aaaattgca acgaaaaat gaatttcca tcaggcgat gcgacgcga gaagcgaac actttcgga ccggtgacc aaagaagtc aacgatctg atcgtcaag cctgaaggg aacaaaagc tgccacatc tgtgccaaa agtttcagc aaaacgcca cagttgagg ctgcatatg aacatccac tacctggag cgtccgttt cgttgccac tcgtgttcg gtcagtttt cgcaccaag ggccacttg cagaagcat gagcgaagt gctagtcat cacaacaaa ctgtcgtca tcgcctgct ttgtcctcg tcggagcct cggccgttt aaatgcgtc gattgtaat atagcgttt cgaattcac ggccaccta gcgaaacat ttgcgctcg aaaatgcac atcatgaaa ttggagtgt ctcgcgaaa ataccgttt ggtttgtac gcagagttg gaaaggtct aacagtctg ttgacggaa attaacact gctgatggg gaccaatgt ttagagagc ttaaagact ttagccaag aaagttttt atcaacgat ccaaataaa ttaaatcaa ttgaattca gcggagagt atagatgcc gatagctag
- >TC009542 RA
aaccgtatc agtatcaaa agcttttct gtggggaag cgctcaagg tgttatctc tatcagtgg tgtttcgca agcggtttc atggggcaa ggggcaaaa cgctgcttg tggtgttac taaagagcg atggaaaac agcgccgcc agttatttg cacaagaag ttcaagaag cagctgagc atcgatccg tcgaaacaa caccaccac cagaatgag agcaaggga gtcgtggac acgcgtcaa gaaaaaacc cccgaacct aatcaaact tgcaaagac aataacgaa agtgtgcca aaccagagt acaaatcaa agcagttct ctctcaaag tcttccata tcagtagag cagtgttgt agcgtgatt ttacaatct tcgaactcg tgcatatcg aaaacctgg accgaacca gtcagcacg tcttctatt ttagataac ttatacaaa gtcaagccc gaagatgta gcttcgaag gaccacgaa gaagccgag aaaaccccc gacaagtcc tcggccggc aaatatgtg tgcccctac tgcaacctg gtgtgctcc aagcccagt gtgttgcag aaacacatc agggcgcac accaacgaa cgcccgttc ccgtgcaac agttgcggg ttcagcttc aagacacgc tcgaatctc tacaaacat tgccggtcg aggacgcac gctaaccgc gtcatgggc aacaaggcg caagagatg ggagaggcg gacgaaggg cccaaaagc ccccagtgc agtgacggc accatcgag gacagagcg caagacctc aagaccaaa ccctacaaa ccacgcttc cacaccaag cacatcttc aaagagaac gaagaaaac aaagacgaa cccaaacac aactccaac atcctctcg gaccacatc aacgaaatc atcaacaag aacaactcg attgtcaac tcgcacgat ccctacttg ctgaagaag aggccagag gtggaggac caccaccag agacacatc accgacttg atttatttg aacaacaac gacgaaccc ttaaacctt acgaaaaac cgcaagcgg tgcatgagc gaagttgtg gaacccgga actcagaag agtttgatc aaagagttg ctcctcaaa aatctaaat tcggatatg caatgccca cattgcaaa atgattttt caaactgtc actgagttg gagttgcac aagctgagg agttgcaaa ggtttcgtc aaacctggt gccaaatac agtcggagt agttcagtc aatgttgcg tcgattctt acgcagaac aagaacgcc tttgacaat ataccgcac ctacagaac gcggtgttt cccctaaaa tcaccgggt cccttcttg ggcaagacc cgattggtc gaaagtgac aaaaacaaa tcgttttca ttcgacgat ggccttccg acagctttc ccaccttcg acacccact gaacgctat ttactctcg cccttgact ttaccgagc gaacgcgaa aaaaagact ccagtgaag ttgttcggt ggtgaagtc aaaatcacg cacacgtcc ggcgaaacg aagagtttc aaaattgat aacaaggac gataagttt gacagtggc tttgtggag tacagtggc aaagtgagt gagaatcgg gtggtgaag tccgggtta cagtcaggt ggtaccgtt ctaaccaac aaagctaac taccccaag gaggattct ttacggaca aatcccgac gttattagg gtgtacgac aacactcca gcctcgcct aacatcgga gtcaaccta accaaaccc caaatcacc tacagggga agtaccgac ccaataata gagtacgcc gtcccgccc tatagcaaa tacacaaac atcgtggat tttagccaa aaagccgta aaactccta acaccgaat ttaaaacaa cccaatctc gcggtcccc ggcgtgcca acccccaac catttcact ttcccaagt ttcactgaa gagaaaata atcgatttg gaaaaaccc gagccgcaa caaaaagtc ttccaaccc aaaatcata actccacaa ccggtctgt aaccccgtc aaactccta gtcaatgga aaagtcgtc cgtcatgtc cctgggatt cccggcccg gtcgtaccc gaagaccaa cccccaccc cggctcaag cccgagccg aaaccgctc gaagtgaaa atcgaccat ccggccgat aaatccccg gttaaaata accgaaacg aaaaaattc gcaaggcct aatagtctc gccttgaaa ccgacaacg gcctctttg aaacaacac cacggtcta acaccgacg ctatttaac cagattttg atcagtcct gacacacct cgtgtggcg aagaaatac gtccatcag tttttgcac gggaattat ttcagttat ttgggtttg aagtgttcg acacggacg gtgtattgt acgctgaat aagactcag ccgttttat gtgcaacat tttaaaaaa ttgagcatg tactccgag tggcggcag caggacacc aagacggag aagttgtac gtgagtgcg tacgattcg aggcagagg catcagagg tacaccacg gcggggaag accacagcg gatttggtg cattcgagt tataagttt gtcgtttcg gagtcttcg agtggcggt gataagtcc gaagaggcg cacaaatcg gccacaatt ctgggcggt tacgaatgc aatgaagac tacacttat atccgaggc agaggccga gggcgttac gtttgtgac cagtgcggg atcaggtgc aaaaaaccg tcaatgttg aagaaacat atcaggacg cattcgaat gacaggccg tatacttgt aatcattgt aattttagt ttcaagaca aaggggaat ttgacgaaa catatgaag agtaaagca cacacaaag aattatgcg gctagtgga tcaggatcg tcgacacaa cagagtggt acacaaagt tctgagtcg gatacggag gacagcggg atggatagt agtgatgaa tcaacacgt cgagaggag catgaagcg gcctacggc ctcctttcc ctctcgcag aaaacctcg cagagcctt cgtcgggag tccacaagt ccaatgtcc acatcgggc tccgacccc caagtttta aacacggaa gaaatagct cacgcttcc aagaccaac tacgcgaaa aacaaattt ctcgatgat aaaacaccg ctcaatttc agcgtccct aaagtcacc actaccacc atcagtagc accaccaca tcatcggac gaagaagtc caaaacttg acccagttc ctaggcaag acgacaatc aatcgacca ctgacttac ccctacact tcgatttta cccccggag tgtaccagt caagaggtg gcggaaaat ccggaaacg aaaagtatc aaacagttc cacgtgata caaaagtac gcctcgagg gaatccccg actgtggac agtagtgcc aattgtttg actagtagt aaaagtgca aaggatagg acacgagtg ccaagtgat cataaagtg aacaatgta ccggttcta aaagtcaac gatgctgtg gtgctcggt gatagtgtg actgatttg aggaaacga aaattgagt tatgcggaa agcgagttc gagagagtg aaacaacac aaaacggac gaagtgatg gacttgtca atgccagtc gagaaaaac cccgaattg aggtacaat ggatcgcct caaccgtac caccataac ctgattaaa aaaaacgaa gatctctac ccgtcatcg aagttcgtc gacgagaaa ttgccggtg aaaaacaac ggtttcgtg agtgtgaac atcaactac gtgataccg gacagtcaa ccgataaac tgtgaatcc cccaaaata gtgtacaaa acggtcgat tatgataac agtgcaatg gaaacgttg gctgacatt gccaccaaa caagtgaaa ctcgagaaa aatactctt gcgaaaagt gtggcgacg gagtttttg aaaattgca acgaaaaat gaatttcca tcaggcgat gcgacgcga gaagcgaac actttcgga ccggtgacc aaagaagtc aacgatctg atcgtcaag cctgaaggg aacaaaagc tgccacatc tgtgccaaa agtttcagc aaaacgcca cagttgagg ctgcatatg aacatccac tacctggag cgtccgttt cgttgccac tcgtgttcg gtcagtttt cgcaccaag ggccacttg cagaagcat gagcgaagt gctagtcat cacaacaaa ctgtcgtca tcgcctgct ttgtcctcg tcggagcct cggccgttt aaatgcgtc gattgtaat atagcgttt cgaattcac ggccaccta gcgaaacat ttgcgctcg aaaatgcac atcatgaaa ttggagtgt ctcgcgaaa ataccgttt ggtttgtac gcagagttg gaaaggtct aacagtctg ttgacggaa attaacact gctgatggg gaccaatgt ttagagagc ttaaagact ttagccaag aaagttttt atcaacgat ccaaataaa ttaaatcaa ttgaattca gcggagagt atagatgcc gatagctag gtaaattaa tttaatttt gtcaagact gaatcaaaa tcttaacaa aaaaagagt atacatttt ataacacta tagtagaaa ttaaatata aaagatata ttttaaatc atctctcat gtgtcaaat atgttatgt gatatatga ataagctag gtagatgtg ttggaatca tgttttagt tggcggcga ttttatttt tacattttg tttttatat gcaagcgac tcccaatcg ttttatttt tatttggtt gtacagtgg gaatcaagt tattttata ttttgatct tgttttaag attatcttc cttaagttg atattatta cctgttttt aggtatttt acatagaga cattattct taagtttta agtgttgta taatagaca atgttaaga taattttca tcgttgtaa atattgttt atgttaggc aatttgtgt actgtttgt agctacatg agttaacac acttctgta atcttcgag gttatgaga ttctcattt gtgccagac actcagctc tattctttt ttaaactgt agtcttgca ggaaattaa acttctttt gtgcaaact gttagttta tattgttga aaaaagtac aagattatt ataattgtt attgatgaa attttgttt taaatataa ctattaatt ttcc
