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Gene information
General information
TC009542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2230877..2238347
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009542
    iBeetle screen iB_04654

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009542 RA
      MENSAASYL HKKFKKQLS IDPSKQHHH QNESKGVVD 
      TRQEKTPEP NQTCKDNNE SVPNQSTNQ SSSLSKSSI 
      SVEQCCSVI LQSSNSCIS KTWTEPVST SSILDNLYK 
      VKPEDVASK DHEEAEKTP DKSSAGKYV CPYCNLVCS 
      KPSVLQKHI RAHTNERPF PCNSCGFSF KTRSNLYKH 
      CRSRTHANR VMGNKAQEM GEADEGPKS PQCSDGTIE 
      DRAQDLKTK PYKPRFHTK HIFKENEEN KDEPKHNSN 
      ILSDHINEI INKNNSIVN SHDPYLLKK RPEVEDHHQ 
      RHITDLIYL NNNDEPLNL TKNRKRCMS EVVEPGTQK 
      SLIKELLLK NLNSDMQCP HCKMIFQTV TELELHKLR 
      SCKGFVKPG AKYSRSSSV NVASILTQN KNAFDNIPH 
      LQNAVFPLK SPGPFLGKT RLVESDKNK SFSFDDGLP 
      TAFPPSTPT ERYLLSPLT LPSEREKKT PVKLFGGEV 
      KITHTSGET KSFKIDNKD DKFDSGFVE YSGKVSENR 
      VVKSGLQSG GTVLTNKAN YPKEDSLRT NPDVIRVYD 
      NTPASPNIG VNLTKPQIT YRGSTDPII EYAVPPYSK 
      YTNIVDFSQ KAVKLLTPN LKQPNLAVP GVPTPNHFT 
      FPSFTEEKI IDLEKPEPQ QKVFQPKII TPQPVCNPV 
      KLLVNGKVV RHVPGIPGP VVPEDQPPP RLKPEPKPL 
      EVKIDHPAD KSPVKITET KKFARPNSL ALKPTTASL 
      KQHHGLTPT LFNQILISP DTPRVAKKY VHQFLHGNY 
      FSYLGLKCS TRTVYCTLN KTQPFYVQH FKKLSMYSE 
      WRQQDTKTE KLYVSAYDS RQRHQRYTT AGKTTADLV 
      HSSYKFVVS ESSSGGDKS EEAHKSATI LGGYECNED 
      YTYIRGRGR GRYVCDQCG IRCKKPSML KKHIRTHSN 
      DRPYTCNHC NFSFKTKGN LTKHMKSKA HTKNYAASG 
      SGSSTQQSG TQSSESDTE DSGMDSSDE STRREEHEA 
      AYGLLSLSQ KTSQSLRRE STSPMSTSG SDPQVLNTE 
      EIAHASKTN YAKNKFLDD KTPLNFSVP KVTTTTISS 
      TTTSSDEEV QNLTQFLGK TTINRPLTY PYTSILPPE 
      CTSQEVAEN PETKSIKQF HVIQKYASR ESPTVDSSA 
      NCLTSSKSA KDRTRVPSD HKVNNVPVL KVNDAVVLG 
      DSVTDLRKR KLSYAESEF ERVKQHKTD EVMDLSMPV 
      EKNPELRYN GSPQPYHHN LIKKNEDLY PSSKFVDEK 
      LPVKNNGFV SVNINYVIP DSQPINCES PKIVYKTVD 
      YDNSAMETL ADIATKQVK LEKNTLAKS VATEFLKIA 
      TKNEFPSGD ATREANTFG PVTKEVNDL IVKPEGNKS 
      CHICAKSFS KTPQLRLHM NIHYLERPF RCHSCSVSF 
      RTKGHLQKH ERSASHHNK LSSSPALSS SEPRPFKCV 
      DCNIAFRIH GHLAKHLRS KMHIMKLEC LAKIPFGLY 
      AELERSNSL LTEINTADG DQCLESLKT LAKKVFIND 
      PNKLNQLNS AESIDADS
    CDS sequence
    • >TC009542 RA
      atggaaaac agcgccgcc agttatttg cacaagaag 
      ttcaagaag cagctgagc atcgatccg tcgaaacaa 
      caccaccac cagaatgag agcaaggga gtcgtggac 
      acgcgtcaa gaaaaaacc cccgaacct aatcaaact 
      tgcaaagac aataacgaa agtgtgcca aaccagagt 
      acaaatcaa agcagttct ctctcaaag tcttccata 
      tcagtagag cagtgttgt agcgtgatt ttacaatct 
      tcgaactcg tgcatatcg aaaacctgg accgaacca 
      gtcagcacg tcttctatt ttagataac ttatacaaa 
      gtcaagccc gaagatgta gcttcgaag gaccacgaa 
      gaagccgag aaaaccccc gacaagtcc tcggccggc 
      aaatatgtg tgcccctac tgcaacctg gtgtgctcc 
      aagcccagt gtgttgcag aaacacatc agggcgcac 
      accaacgaa cgcccgttc ccgtgcaac agttgcggg 
      ttcagcttc aagacacgc tcgaatctc tacaaacat 
      tgccggtcg aggacgcac gctaaccgc gtcatgggc 
      aacaaggcg caagagatg ggagaggcg gacgaaggg 
      cccaaaagc ccccagtgc agtgacggc accatcgag 
      gacagagcg caagacctc aagaccaaa ccctacaaa 
      ccacgcttc cacaccaag cacatcttc aaagagaac 
      gaagaaaac aaagacgaa cccaaacac aactccaac 
      atcctctcg gaccacatc aacgaaatc atcaacaag 
      aacaactcg attgtcaac tcgcacgat ccctacttg 
      ctgaagaag aggccagag gtggaggac caccaccag 
      agacacatc accgacttg atttatttg aacaacaac 
      gacgaaccc ttaaacctt acgaaaaac cgcaagcgg 
      tgcatgagc gaagttgtg gaacccgga actcagaag 
      agtttgatc aaagagttg ctcctcaaa aatctaaat 
      tcggatatg caatgccca cattgcaaa atgattttt 
      caaactgtc actgagttg gagttgcac aagctgagg 
      agttgcaaa ggtttcgtc aaacctggt gccaaatac 
      agtcggagt agttcagtc aatgttgcg tcgattctt 
      acgcagaac aagaacgcc tttgacaat ataccgcac 
      ctacagaac gcggtgttt cccctaaaa tcaccgggt 
      cccttcttg ggcaagacc cgattggtc gaaagtgac 
      aaaaacaaa tcgttttca ttcgacgat ggccttccg 
      acagctttc ccaccttcg acacccact gaacgctat 
      ttactctcg cccttgact ttaccgagc gaacgcgaa 
      aaaaagact ccagtgaag ttgttcggt ggtgaagtc 
      aaaatcacg cacacgtcc ggcgaaacg aagagtttc 
      aaaattgat aacaaggac gataagttt gacagtggc 
      tttgtggag tacagtggc aaagtgagt gagaatcgg 
      gtggtgaag tccgggtta cagtcaggt ggtaccgtt 
      ctaaccaac aaagctaac taccccaag gaggattct 
      ttacggaca aatcccgac gttattagg gtgtacgac 
      aacactcca gcctcgcct aacatcgga gtcaaccta 
      accaaaccc caaatcacc tacagggga agtaccgac 
      ccaataata gagtacgcc gtcccgccc tatagcaaa 
      tacacaaac atcgtggat tttagccaa aaagccgta 
      aaactccta acaccgaat ttaaaacaa cccaatctc 
      gcggtcccc ggcgtgcca acccccaac catttcact 
      ttcccaagt ttcactgaa gagaaaata atcgatttg 
      gaaaaaccc gagccgcaa caaaaagtc ttccaaccc 
      aaaatcata actccacaa ccggtctgt aaccccgtc 
      aaactccta gtcaatgga aaagtcgtc cgtcatgtc 
      cctgggatt cccggcccg gtcgtaccc gaagaccaa 
      cccccaccc cggctcaag cccgagccg aaaccgctc 
      gaagtgaaa atcgaccat ccggccgat aaatccccg 
      gttaaaata accgaaacg aaaaaattc gcaaggcct 
      aatagtctc gccttgaaa ccgacaacg gcctctttg 
      aaacaacac cacggtcta acaccgacg ctatttaac 
      cagattttg atcagtcct gacacacct cgtgtggcg 
      aagaaatac gtccatcag tttttgcac gggaattat 
      ttcagttat ttgggtttg aagtgttcg acacggacg 
      gtgtattgt acgctgaat aagactcag ccgttttat 
      gtgcaacat tttaaaaaa ttgagcatg tactccgag 
      tggcggcag caggacacc aagacggag aagttgtac 
      gtgagtgcg tacgattcg aggcagagg catcagagg 
      tacaccacg gcggggaag accacagcg gatttggtg 
      cattcgagt tataagttt gtcgtttcg gagtcttcg 
      agtggcggt gataagtcc gaagaggcg cacaaatcg 
      gccacaatt ctgggcggt tacgaatgc aatgaagac 
      tacacttat atccgaggc agaggccga gggcgttac 
      gtttgtgac cagtgcggg atcaggtgc aaaaaaccg 
      tcaatgttg aagaaacat atcaggacg cattcgaat 
      gacaggccg tatacttgt aatcattgt aattttagt 
      ttcaagaca aaggggaat ttgacgaaa catatgaag 
      agtaaagca cacacaaag aattatgcg gctagtgga 
      tcaggatcg tcgacacaa cagagtggt acacaaagt 
      tctgagtcg gatacggag gacagcggg atggatagt 
      agtgatgaa tcaacacgt cgagaggag catgaagcg 
      gcctacggc ctcctttcc ctctcgcag aaaacctcg 
      cagagcctt cgtcgggag tccacaagt ccaatgtcc 
      acatcgggc tccgacccc caagtttta aacacggaa 
      gaaatagct cacgcttcc aagaccaac tacgcgaaa 
      aacaaattt ctcgatgat aaaacaccg ctcaatttc 
      agcgtccct aaagtcacc actaccacc atcagtagc 
      accaccaca tcatcggac gaagaagtc caaaacttg 
      acccagttc ctaggcaag acgacaatc aatcgacca 
      ctgacttac ccctacact tcgatttta cccccggag 
      tgtaccagt caagaggtg gcggaaaat ccggaaacg 
      aaaagtatc aaacagttc cacgtgata caaaagtac 
      gcctcgagg gaatccccg actgtggac agtagtgcc 
      aattgtttg actagtagt aaaagtgca aaggatagg 
      acacgagtg ccaagtgat cataaagtg aacaatgta 
      ccggttcta aaagtcaac gatgctgtg gtgctcggt 
      gatagtgtg actgatttg aggaaacga aaattgagt 
      tatgcggaa agcgagttc gagagagtg aaacaacac 
      aaaacggac gaagtgatg gacttgtca atgccagtc 
      gagaaaaac cccgaattg aggtacaat ggatcgcct 
      caaccgtac caccataac ctgattaaa aaaaacgaa 
      gatctctac ccgtcatcg aagttcgtc gacgagaaa 
      ttgccggtg aaaaacaac ggtttcgtg agtgtgaac 
      atcaactac gtgataccg gacagtcaa ccgataaac 
      tgtgaatcc cccaaaata gtgtacaaa acggtcgat 
      tatgataac agtgcaatg gaaacgttg gctgacatt 
      gccaccaaa caagtgaaa ctcgagaaa aatactctt 
      gcgaaaagt gtggcgacg gagtttttg aaaattgca 
      acgaaaaat gaatttcca tcaggcgat gcgacgcga 
      gaagcgaac actttcgga ccggtgacc aaagaagtc 
      aacgatctg atcgtcaag cctgaaggg aacaaaagc 
      tgccacatc tgtgccaaa agtttcagc aaaacgcca 
      cagttgagg ctgcatatg aacatccac tacctggag 
      cgtccgttt cgttgccac tcgtgttcg gtcagtttt 
      cgcaccaag ggccacttg cagaagcat gagcgaagt 
      gctagtcat cacaacaaa ctgtcgtca tcgcctgct 
      ttgtcctcg tcggagcct cggccgttt aaatgcgtc 
      gattgtaat atagcgttt cgaattcac ggccaccta 
      gcgaaacat ttgcgctcg aaaatgcac atcatgaaa 
      ttggagtgt ctcgcgaaa ataccgttt ggtttgtac 
      gcagagttg gaaaggtct aacagtctg ttgacggaa 
      attaacact gctgatggg gaccaatgt ttagagagc 
      ttaaagact ttagccaag aaagttttt atcaacgat 
      ccaaataaa ttaaatcaa ttgaattca gcggagagt 
      atagatgcc gatagctag
    mRNA sequence
    • >TC009542 RA
      aaccgtatc agtatcaaa agcttttct gtggggaag 
      cgctcaagg tgttatctc tatcagtgg tgtttcgca 
      agcggtttc atggggcaa ggggcaaaa cgctgcttg 
      tggtgttac taaagagcg atggaaaac agcgccgcc 
      agttatttg cacaagaag ttcaagaag cagctgagc 
      atcgatccg tcgaaacaa caccaccac cagaatgag 
      agcaaggga gtcgtggac acgcgtcaa gaaaaaacc 
      cccgaacct aatcaaact tgcaaagac aataacgaa 
      agtgtgcca aaccagagt acaaatcaa agcagttct 
      ctctcaaag tcttccata tcagtagag cagtgttgt 
      agcgtgatt ttacaatct tcgaactcg tgcatatcg 
      aaaacctgg accgaacca gtcagcacg tcttctatt 
      ttagataac ttatacaaa gtcaagccc gaagatgta 
      gcttcgaag gaccacgaa gaagccgag aaaaccccc 
      gacaagtcc tcggccggc aaatatgtg tgcccctac 
      tgcaacctg gtgtgctcc aagcccagt gtgttgcag 
      aaacacatc agggcgcac accaacgaa cgcccgttc 
      ccgtgcaac agttgcggg ttcagcttc aagacacgc 
      tcgaatctc tacaaacat tgccggtcg aggacgcac 
      gctaaccgc gtcatgggc aacaaggcg caagagatg 
      ggagaggcg gacgaaggg cccaaaagc ccccagtgc 
      agtgacggc accatcgag gacagagcg caagacctc 
      aagaccaaa ccctacaaa ccacgcttc cacaccaag 
      cacatcttc aaagagaac gaagaaaac aaagacgaa 
      cccaaacac aactccaac atcctctcg gaccacatc 
      aacgaaatc atcaacaag aacaactcg attgtcaac 
      tcgcacgat ccctacttg ctgaagaag aggccagag 
      gtggaggac caccaccag agacacatc accgacttg 
      atttatttg aacaacaac gacgaaccc ttaaacctt 
      acgaaaaac cgcaagcgg tgcatgagc gaagttgtg 
      gaacccgga actcagaag agtttgatc aaagagttg 
      ctcctcaaa aatctaaat tcggatatg caatgccca 
      cattgcaaa atgattttt caaactgtc actgagttg 
      gagttgcac aagctgagg agttgcaaa ggtttcgtc 
      aaacctggt gccaaatac agtcggagt agttcagtc 
      aatgttgcg tcgattctt acgcagaac aagaacgcc 
      tttgacaat ataccgcac ctacagaac gcggtgttt 
      cccctaaaa tcaccgggt cccttcttg ggcaagacc 
      cgattggtc gaaagtgac aaaaacaaa tcgttttca 
      ttcgacgat ggccttccg acagctttc ccaccttcg 
      acacccact gaacgctat ttactctcg cccttgact 
      ttaccgagc gaacgcgaa aaaaagact ccagtgaag 
      ttgttcggt ggtgaagtc aaaatcacg cacacgtcc 
      ggcgaaacg aagagtttc aaaattgat aacaaggac 
      gataagttt gacagtggc tttgtggag tacagtggc 
      aaagtgagt gagaatcgg gtggtgaag tccgggtta 
      cagtcaggt ggtaccgtt ctaaccaac aaagctaac 
      taccccaag gaggattct ttacggaca aatcccgac 
      gttattagg gtgtacgac aacactcca gcctcgcct 
      aacatcgga gtcaaccta accaaaccc caaatcacc 
      tacagggga agtaccgac ccaataata gagtacgcc 
      gtcccgccc tatagcaaa tacacaaac atcgtggat 
      tttagccaa aaagccgta aaactccta acaccgaat 
      ttaaaacaa cccaatctc gcggtcccc ggcgtgcca 
      acccccaac catttcact ttcccaagt ttcactgaa 
      gagaaaata atcgatttg gaaaaaccc gagccgcaa 
      caaaaagtc ttccaaccc aaaatcata actccacaa 
      ccggtctgt aaccccgtc aaactccta gtcaatgga 
      aaagtcgtc cgtcatgtc cctgggatt cccggcccg 
      gtcgtaccc gaagaccaa cccccaccc cggctcaag 
      cccgagccg aaaccgctc gaagtgaaa atcgaccat 
      ccggccgat aaatccccg gttaaaata accgaaacg 
      aaaaaattc gcaaggcct aatagtctc gccttgaaa 
      ccgacaacg gcctctttg aaacaacac cacggtcta 
      acaccgacg ctatttaac cagattttg atcagtcct 
      gacacacct cgtgtggcg aagaaatac gtccatcag 
      tttttgcac gggaattat ttcagttat ttgggtttg 
      aagtgttcg acacggacg gtgtattgt acgctgaat 
      aagactcag ccgttttat gtgcaacat tttaaaaaa 
      ttgagcatg tactccgag tggcggcag caggacacc 
      aagacggag aagttgtac gtgagtgcg tacgattcg 
      aggcagagg catcagagg tacaccacg gcggggaag 
      accacagcg gatttggtg cattcgagt tataagttt 
      gtcgtttcg gagtcttcg agtggcggt gataagtcc 
      gaagaggcg cacaaatcg gccacaatt ctgggcggt 
      tacgaatgc aatgaagac tacacttat atccgaggc 
      agaggccga gggcgttac gtttgtgac cagtgcggg 
      atcaggtgc aaaaaaccg tcaatgttg aagaaacat 
      atcaggacg cattcgaat gacaggccg tatacttgt 
      aatcattgt aattttagt ttcaagaca aaggggaat 
      ttgacgaaa catatgaag agtaaagca cacacaaag 
      aattatgcg gctagtgga tcaggatcg tcgacacaa 
      cagagtggt acacaaagt tctgagtcg gatacggag 
      gacagcggg atggatagt agtgatgaa tcaacacgt 
      cgagaggag catgaagcg gcctacggc ctcctttcc 
      ctctcgcag aaaacctcg cagagcctt cgtcgggag 
      tccacaagt ccaatgtcc acatcgggc tccgacccc 
      caagtttta aacacggaa gaaatagct cacgcttcc 
      aagaccaac tacgcgaaa aacaaattt ctcgatgat 
      aaaacaccg ctcaatttc agcgtccct aaagtcacc 
      actaccacc atcagtagc accaccaca tcatcggac 
      gaagaagtc caaaacttg acccagttc ctaggcaag 
      acgacaatc aatcgacca ctgacttac ccctacact 
      tcgatttta cccccggag tgtaccagt caagaggtg 
      gcggaaaat ccggaaacg aaaagtatc aaacagttc 
      cacgtgata caaaagtac gcctcgagg gaatccccg 
      actgtggac agtagtgcc aattgtttg actagtagt 
      aaaagtgca aaggatagg acacgagtg ccaagtgat 
      cataaagtg aacaatgta ccggttcta aaagtcaac 
      gatgctgtg gtgctcggt gatagtgtg actgatttg 
      aggaaacga aaattgagt tatgcggaa agcgagttc 
      gagagagtg aaacaacac aaaacggac gaagtgatg 
      gacttgtca atgccagtc gagaaaaac cccgaattg 
      aggtacaat ggatcgcct caaccgtac caccataac 
      ctgattaaa aaaaacgaa gatctctac ccgtcatcg 
      aagttcgtc gacgagaaa ttgccggtg aaaaacaac 
      ggtttcgtg agtgtgaac atcaactac gtgataccg 
      gacagtcaa ccgataaac tgtgaatcc cccaaaata 
      gtgtacaaa acggtcgat tatgataac agtgcaatg 
      gaaacgttg gctgacatt gccaccaaa caagtgaaa 
      ctcgagaaa aatactctt gcgaaaagt gtggcgacg 
      gagtttttg aaaattgca acgaaaaat gaatttcca 
      tcaggcgat gcgacgcga gaagcgaac actttcgga 
      ccggtgacc aaagaagtc aacgatctg atcgtcaag 
      cctgaaggg aacaaaagc tgccacatc tgtgccaaa 
      agtttcagc aaaacgcca cagttgagg ctgcatatg 
      aacatccac tacctggag cgtccgttt cgttgccac 
      tcgtgttcg gtcagtttt cgcaccaag ggccacttg 
      cagaagcat gagcgaagt gctagtcat cacaacaaa 
      ctgtcgtca tcgcctgct ttgtcctcg tcggagcct 
      cggccgttt aaatgcgtc gattgtaat atagcgttt 
      cgaattcac ggccaccta gcgaaacat ttgcgctcg 
      aaaatgcac atcatgaaa ttggagtgt ctcgcgaaa 
      ataccgttt ggtttgtac gcagagttg gaaaggtct 
      aacagtctg ttgacggaa attaacact gctgatggg 
      gaccaatgt ttagagagc ttaaagact ttagccaag 
      aaagttttt atcaacgat ccaaataaa ttaaatcaa 
      ttgaattca gcggagagt atagatgcc gatagctag 
      gtaaattaa tttaatttt gtcaagact gaatcaaaa 
      tcttaacaa aaaaagagt atacatttt ataacacta 
      tagtagaaa ttaaatata aaagatata ttttaaatc 
      atctctcat gtgtcaaat atgttatgt gatatatga 
      ataagctag gtagatgtg ttggaatca tgttttagt 
      tggcggcga ttttatttt tacattttg tttttatat 
      gcaagcgac tcccaatcg ttttatttt tatttggtt 
      gtacagtgg gaatcaagt tattttata ttttgatct 
      tgttttaag attatcttc cttaagttg atattatta 
      cctgttttt aggtatttt acatagaga cattattct 
      taagtttta agtgttgta taatagaca atgttaaga 
      taattttca tcgttgtaa atattgttt atgttaggc 
      aatttgtgt actgtttgt agctacatg agttaacac 
      acttctgta atcttcgag gttatgaga ttctcattt 
      gtgccagac actcagctc tattctttt ttaaactgt 
      agtcttgca ggaaattaa acttctttt gtgcaaact 
      gttagttta tattgttga aaaaagtac aagattatt 
      ataattgtt attgatgaa attttgttt taaatataa 
      ctattaatt ttcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY