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Gene information
General information
TC009525 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1926945..1931344
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009525
    iBeetle screen iB_04650

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009525 RA
      MFGGSLDPP KHAKRSRPN PMTYDIQLP CITPGVYGH 
      RGRQPNTDH HVRLLHYRQ HLRDADSEF LASEAKRLL 
      KETVTAKYD KHAEKKVFQ RHIQERFKE KVKAYEETI 
      EKRRLKLKE LLCREEQEF IIETIDKAQ KGVDSKMEE 
      MKNKAQLLK AQREAERLE IVNQKRIQQ YIDGCVELR 
      PVVYQRNLI DSKNAQLQQ MKENQMRKE SERELEVMW 
      HNLMKKEQE AKMEKEVQD LLKQNRAMQ ETMQTWEKQ 
      IHGKELLKQ EAEKVAAED RIEMIKLQE QIRREEIEE 
      LDAKRRKRD QVAKELREQ IEVQERFIA QRKKEEEAL 
      NNAMSRLAE VELEKERQK IKDTTSLAK RETAIYRNH 
      LKELEVERK REEEQLDKL LEEHRKMIE KKQEEAFCK 
      LKKARQELQ KNVLAERAQ QLEYKRQEA ENQLRLKEA 
      ENELLRMAW ESNERLQAE HDRLQALQV KQYREDLRQ 
      QIEYNNEQR KLEKKKLER ELAEGLAEE ERRRQHVLE 
      LIDARLHDP AFQHPFRRV LERPSCPCP NPTT
    CDS sequence
    • >TC009525 RA
      atgtttggg ggcagcttg gaccccccg aaacatgcg 
      aaacggtca agacccaac cccatgacc tacgacata 
      caactgcca tgcataaca ccaggagtt tacggacat 
      cgaggtcgt caaccgaat accgaccac cacgtccgc 
      ttgttacac taccggcag catctccgt gatgccgac 
      agtgaattt ctagcttcc gaagccaaa cgactgctc 
      aaagagact gtgacagcc aaatacgac aaacatgcc 
      gaaaaaaag gtcttccaa agacatatc caggaacga 
      ttcaaggag aaagttaaa gcttacgaa gagacaatc 
      gagaagaga cgtctaaag ttgaaggaa ctgttgtgc 
      cgtgaagaa caagaattt ataattgaa actattgac 
      aaagcccag aaaggcgtt gattccaaa atggaagag 
      atgaaaaat aaagcacag ttgttgaaa gcgcagcgt 
      gaagctgaa cgacttgaa attgtaaac cagaaacgg 
      atccagcaa tacattgac ggttgcgtc gagttgcgc 
      ccagtcgtc taccaacga aacctcata gacagcaaa 
      aacgcccaa ttgcaacaa atgaaggaa aatcagatg 
      cgaaaagag tcggaacgc gagcttgaa gtcatgtgg 
      cacaacctc atgaagaaa gaacaagaa gctaagatg 
      gagaaagaa gtgcaagat ttgcttaaa caaaaccgg 
      gcgatgcaa gagaccatg caaacgtgg gagaagcaa 
      atccacggc aaggaactc ctgaagcaa gaggcggaa 
      aaagtggcc gccgaagac cgcatcgag atgattaaa 
      ctccaagag cagattcgg cgcgaagag attgaagaa 
      ttggacgcc aagcggcga aagcgcgac caagtggcc 
      aaagaactg agggagcaa atcgaagtc caggagcgg 
      tttatcgcg caacggaag aaagaggag gaagctttg 
      aacaatgca atgagtcgc ttggccgaa gtggagttg 
      gagaaggag aggcagaaa attaaggac acaaccagt 
      ttggcgaaa cgtgaaact gcgatctac cgcaaccat 
      ttgaaggag ttggaggtg gagaggaag agggaggag 
      gagcagttg gataagctg ttggaagag caccgcaaa 
      atgattgag aagaagcag gaggaggct ttttgcaag 
      ttgaagaaa gcgcgtcaa gagttgcaa aagaacgtt 
      ttggcagaa agggcccaa cagttggag tacaagcga 
      caggaggct gagaatcaa ctcaggctg aaagaggct 
      gagaatgag ctgttgagg atggcttgg gagagcaac 
      gagcggctt caggcggaa catgaccgc ctacaggcc 
      cttcaggtc aagcaatat cgcgaggat ttgcggcaa 
      cagattgaa tataacaac gagcaaagg aaacttgag 
      aaaaaaaaa ctggaacgg gagctagct gagggactt 
      gctgaggaa gagcgccgc cgccaacac gtcctggag 
      ctgattgat gccagacta cacgacccc gctttccag 
      catccgttc cgaagagtt ttagaacgt ccgagctgt 
      ccttgcccc aacccaacc acctga
    mRNA sequence
    • >TC009525 RA
      tcttaacag tcacttgga caagttgga gatgtttgg 
      gggcagctt ggacccccc gaaacatgc gaaacggtc 
      aagacccaa ccccatgac ctacgacat acaactgcc 
      atgcataac accaggagt ttacggaca tcgaggtcg 
      tcaaccgaa taccgacca ccacgtccg cttgttaca 
      ctaccggca gcatctccg tgatgccga cagtgaatt 
      tctagcttc cgaagccaa acgactgct caaagagac 
      tgtgacagc caaatacga caaacatgc cgaaaaaaa 
      ggtcttcca aagacatat ccaggaacg attcaagga 
      gaaagttaa agcttacga agagacaat cgagaagag 
      acgtctaaa gttgaagga actgttgtg ccgtgaaga 
      acaagaatt tataattga aactattga caaagccca 
      gaaaggcgt tgattccaa aatggaaga gatgaaaaa 
      taaagcaca gttgttgaa agcgcagcg tgaagctga 
      acgacttga aattgtaaa ccagaaacg gatccagca 
      atacattga cggttgcgt cgagttgcg cccagtcgt 
      ctaccaacg aaacctcat agacagcaa aaacgccca 
      attgcaaca aatgaagga aaatcagat gcgaaaaga 
      gtcggaacg cgagcttga agtcatgtg gcacaacct 
      catgaagaa agaacaaga agctaagat ggagaaaga 
      agtgcaaga tttgcttaa acaaaaccg ggcgatgca 
      agagaccat gcaaacgtg ggagaagca aatccacgg 
      caaggaact cctgaagca agaggcgga aaaagtggc 
      cgccgaaga ccgcatcga gatgattaa actccaaga 
      gcagattcg gcgcgaaga gattgaaga attggacgc 
      caagcggcg aaagcgcga ccaagtggc caaagaact 
      gagggagca aatcgaagt ccaggagcg gtttatcgc 
      gcaacggaa gaaagagga ggaagcttt gaacaatgc 
      aatgagtcg cttggccga agtggagtt ggagaagga 
      gaggcagaa aattaagga cacaaccag tttggcgaa 
      acgtgaaac tgcgatcta ccgcaacca tttgaagga 
      gttggaggt ggagaggaa gagggagga ggagcagtt 
      ggataagct gttggaaga gcaccgcaa aatgattga 
      gaagaagca ggaggaggc tttttgcaa gttgaagaa 
      agcgcgtca agagttgca aaagaacgt tttggcaga 
      aagggccca acagttgga gtacaagcg acaggaggc 
      tgagaatca actcaggct gaaagaggc tgagaatga 
      gctgttgag gatggcttg ggagagcaa cgagcggct 
      tcaggcgga acatgaccg cctacaggc ccttcaggt 
      caagcaata tcgcgagga tttgcggca acagattga 
      atataacaa cgagcaaag gaaacttga gaaaaaaaa 
      actggaacg ggagctagc tgagggact tgctgagga 
      agagcgccg ccgccaaca cgtcctgga gctgattga 
      tgccagact acacgaccc cgctttcca gcatccgtt 
      ccgaagagt tttagaacg tccgagctg tccttgccc 
      caacccaac cacctgaaa tgacttgta caacaaaag 
      agacattac agaattatt tgaaagttt tatttgttt 
      tattgctta atgccagtg acgggaact aattttcgc 
      caagattct cgttaaaac ttagcgaca atccaagtt 
      tattggctt tgaagtcgt tgcacaacg agtcggaat 
      tgcataagg agtatgtga tgttcaatt agaacgtat 
      tttacggtg accacattc actgattta acctcgttt 
      attctgtaa gaagacacc cctgggttt tgcataaat 
      cgcctcagg tgcgaaagt agaagcaac tggagcgcc 
      gtgattagt ttaaccttt taatttgtg caagatgaa 
      tttatgcaa ttgtaactg gtctgaagg tttccaaga 
      ggaaataat aattagtca atcgtttgt aaagtaaca 
      aacgctcgg gatttacag aatgggaaa aaattcgta 
      accaaatca cagtcgtag aatggacaa gagtgaaag 
      ttgatggta ttgttgatc aataataaa taattgcac 
      gtggtgtgt gccggaagc gaggcgtgt aaatggaaa 
      catttttcg attttaatg agcggttat gttttatgg 
      gctctgtgg cgattactt gggcaaata atgagtgtg 
      gtttcactt atgaggtga taaaagctc attaaagtt 
      gcaaaaaac aaattgatt gaaatttat atactgatg 
      acaataaag tgttgttgc gcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY