| General information | |||
| TC009525 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1926945..1931344 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009525 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04650 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC009525 RA
MFGGSLDPP KHAKRSRPN PMTYDIQLP CITPGVYGH RGRQPNTDH HVRLLHYRQ HLRDADSEF LASEAKRLL KETVTAKYD KHAEKKVFQ RHIQERFKE KVKAYEETI EKRRLKLKE LLCREEQEF IIETIDKAQ KGVDSKMEE MKNKAQLLK AQREAERLE IVNQKRIQQ YIDGCVELR PVVYQRNLI DSKNAQLQQ MKENQMRKE SERELEVMW HNLMKKEQE AKMEKEVQD LLKQNRAMQ ETMQTWEKQ IHGKELLKQ EAEKVAAED RIEMIKLQE QIRREEIEE LDAKRRKRD QVAKELREQ IEVQERFIA QRKKEEEAL NNAMSRLAE VELEKERQK IKDTTSLAK RETAIYRNH LKELEVERK REEEQLDKL LEEHRKMIE KKQEEAFCK LKKARQELQ KNVLAERAQ QLEYKRQEA ENQLRLKEA ENELLRMAW ESNERLQAE HDRLQALQV KQYREDLRQ QIEYNNEQR KLEKKKLER ELAEGLAEE ERRRQHVLE LIDARLHDP AFQHPFRRV LERPSCPCP NPTT
- >TC009525 RA
atgtttggg ggcagcttg gaccccccg aaacatgcg aaacggtca agacccaac cccatgacc tacgacata caactgcca tgcataaca ccaggagtt tacggacat cgaggtcgt caaccgaat accgaccac cacgtccgc ttgttacac taccggcag catctccgt gatgccgac agtgaattt ctagcttcc gaagccaaa cgactgctc aaagagact gtgacagcc aaatacgac aaacatgcc gaaaaaaag gtcttccaa agacatatc caggaacga ttcaaggag aaagttaaa gcttacgaa gagacaatc gagaagaga cgtctaaag ttgaaggaa ctgttgtgc cgtgaagaa caagaattt ataattgaa actattgac aaagcccag aaaggcgtt gattccaaa atggaagag atgaaaaat aaagcacag ttgttgaaa gcgcagcgt gaagctgaa cgacttgaa attgtaaac cagaaacgg atccagcaa tacattgac ggttgcgtc gagttgcgc ccagtcgtc taccaacga aacctcata gacagcaaa aacgcccaa ttgcaacaa atgaaggaa aatcagatg cgaaaagag tcggaacgc gagcttgaa gtcatgtgg cacaacctc atgaagaaa gaacaagaa gctaagatg gagaaagaa gtgcaagat ttgcttaaa caaaaccgg gcgatgcaa gagaccatg caaacgtgg gagaagcaa atccacggc aaggaactc ctgaagcaa gaggcggaa aaagtggcc gccgaagac cgcatcgag atgattaaa ctccaagag cagattcgg cgcgaagag attgaagaa ttggacgcc aagcggcga aagcgcgac caagtggcc aaagaactg agggagcaa atcgaagtc caggagcgg tttatcgcg caacggaag aaagaggag gaagctttg aacaatgca atgagtcgc ttggccgaa gtggagttg gagaaggag aggcagaaa attaaggac acaaccagt ttggcgaaa cgtgaaact gcgatctac cgcaaccat ttgaaggag ttggaggtg gagaggaag agggaggag gagcagttg gataagctg ttggaagag caccgcaaa atgattgag aagaagcag gaggaggct ttttgcaag ttgaagaaa gcgcgtcaa gagttgcaa aagaacgtt ttggcagaa agggcccaa cagttggag tacaagcga caggaggct gagaatcaa ctcaggctg aaagaggct gagaatgag ctgttgagg atggcttgg gagagcaac gagcggctt caggcggaa catgaccgc ctacaggcc cttcaggtc aagcaatat cgcgaggat ttgcggcaa cagattgaa tataacaac gagcaaagg aaacttgag aaaaaaaaa ctggaacgg gagctagct gagggactt gctgaggaa gagcgccgc cgccaacac gtcctggag ctgattgat gccagacta cacgacccc gctttccag catccgttc cgaagagtt ttagaacgt ccgagctgt ccttgcccc aacccaacc acctga
- >TC009525 RA
tcttaacag tcacttgga caagttgga gatgtttgg gggcagctt ggacccccc gaaacatgc gaaacggtc aagacccaa ccccatgac ctacgacat acaactgcc atgcataac accaggagt ttacggaca tcgaggtcg tcaaccgaa taccgacca ccacgtccg cttgttaca ctaccggca gcatctccg tgatgccga cagtgaatt tctagcttc cgaagccaa acgactgct caaagagac tgtgacagc caaatacga caaacatgc cgaaaaaaa ggtcttcca aagacatat ccaggaacg attcaagga gaaagttaa agcttacga agagacaat cgagaagag acgtctaaa gttgaagga actgttgtg ccgtgaaga acaagaatt tataattga aactattga caaagccca gaaaggcgt tgattccaa aatggaaga gatgaaaaa taaagcaca gttgttgaa agcgcagcg tgaagctga acgacttga aattgtaaa ccagaaacg gatccagca atacattga cggttgcgt cgagttgcg cccagtcgt ctaccaacg aaacctcat agacagcaa aaacgccca attgcaaca aatgaagga aaatcagat gcgaaaaga gtcggaacg cgagcttga agtcatgtg gcacaacct catgaagaa agaacaaga agctaagat ggagaaaga agtgcaaga tttgcttaa acaaaaccg ggcgatgca agagaccat gcaaacgtg ggagaagca aatccacgg caaggaact cctgaagca agaggcgga aaaagtggc cgccgaaga ccgcatcga gatgattaa actccaaga gcagattcg gcgcgaaga gattgaaga attggacgc caagcggcg aaagcgcga ccaagtggc caaagaact gagggagca aatcgaagt ccaggagcg gtttatcgc gcaacggaa gaaagagga ggaagcttt gaacaatgc aatgagtcg cttggccga agtggagtt ggagaagga gaggcagaa aattaagga cacaaccag tttggcgaa acgtgaaac tgcgatcta ccgcaacca tttgaagga gttggaggt ggagaggaa gagggagga ggagcagtt ggataagct gttggaaga gcaccgcaa aatgattga gaagaagca ggaggaggc tttttgcaa gttgaagaa agcgcgtca agagttgca aaagaacgt tttggcaga aagggccca acagttgga gtacaagcg acaggaggc tgagaatca actcaggct gaaagaggc tgagaatga gctgttgag gatggcttg ggagagcaa cgagcggct tcaggcgga acatgaccg cctacaggc ccttcaggt caagcaata tcgcgagga tttgcggca acagattga atataacaa cgagcaaag gaaacttga gaaaaaaaa actggaacg ggagctagc tgagggact tgctgagga agagcgccg ccgccaaca cgtcctgga gctgattga tgccagact acacgaccc cgctttcca gcatccgtt ccgaagagt tttagaacg tccgagctg tccttgccc caacccaac cacctgaaa tgacttgta caacaaaag agacattac agaattatt tgaaagttt tatttgttt tattgctta atgccagtg acgggaact aattttcgc caagattct cgttaaaac ttagcgaca atccaagtt tattggctt tgaagtcgt tgcacaacg agtcggaat tgcataagg agtatgtga tgttcaatt agaacgtat tttacggtg accacattc actgattta acctcgttt attctgtaa gaagacacc cctgggttt tgcataaat cgcctcagg tgcgaaagt agaagcaac tggagcgcc gtgattagt ttaaccttt taatttgtg caagatgaa tttatgcaa ttgtaactg gtctgaagg tttccaaga ggaaataat aattagtca atcgtttgt aaagtaaca aacgctcgg gatttacag aatgggaaa aaattcgta accaaatca cagtcgtag aatggacaa gagtgaaag ttgatggta ttgttgatc aataataaa taattgcac gtggtgtgt gccggaagc gaggcgtgt aaatggaaa catttttcg attttaatg agcggttat gttttatgg gctctgtgg cgattactt gggcaaata atgagtgtg gtttcactt atgaggtga taaaagctc attaaagtt gcaaaaaac aaattgatt gaaatttat atactgatg acaataaag tgttgttgc gcta
