Status
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Gene information
General information
TC009516 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1802373..1805476
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009516
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
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      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04647

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009516 RA
        MNVRERLKE AYESVSFSA DRTWASVPT AFHACNLDL 
        HLTEKVLQS QKLHRFRPR TVREAIPKS RLAQYVNCV 
        YNESREMSY AANSALNET LAQSGEPTE AWEMPEVRE 
        LYKWFLKQP EDDFYRVDR RYESPLLNV KGFEDKILK 
        IQITEDRVK NIWNSDFYQ IGEPRIKNE QIIVNFFTS 
        PMSNVFLFD NETGYDYEW FRHISLTKD FDQLEQLST 
        SFKDQINSI AKFMSSSNE PPPPSEPIS TEILYSEVL 
        QGLSPSRKL LSSFKCDHN NKDETSSTS QESFTNYTI 
        LNQKAGQED GKQARHYKP SQVDSVQRN TRASVHPCV 
        QQAYFYSPA VQRWSYYPT QVFTSSWVA QSPVVNRLF 
        GASPVTIAS ESENQVEGE EHQVSTSIS EELEIQALE 
        QYSNSNENF YQELERQAA EQYANSPE
      CDS sequence
      • >TC009516 RA
        atgaacgta agggaacgt ctgaaagag gcatatgag 
        agcgtttct ttcagtgcc gatagaacc tgggcaagc 
        gtgccaacg gcattccac gcgtgtaac ttggatttg 
        cacttgact gaaaaggtc ctgcaaagt cagaagttg 
        catcgattt cggcctcgc accgtcaga gaggcgatt 
        ccgaagagc agattggct cagtatgtg aattgcgtc 
        tacaatgag tcgagggaa atgtcatac gctgcgaac 
        agtgccctt aatgaaaca ctcgcacag agtggagaa 
        ccgacggaa gcctgggag atgcccgaa gtgagggag 
        ctgtacaag tggttcttg aaacagcct gaagatgat 
        ttttacaga gttgacaga cggtatgaa tcgccgttg 
        ctcaacgtc aaaggcttc gaagacaag attttgaaa 
        atacaaatc accgaagac agagttaaa aatatctgg 
        aacagcgat ttttaccaa atcggtgaa ccccgcatt 
        aaaaacgag caaatcata gttaatttc tttacgtcc 
        ccgatgagc aacgtgttt cttttcgac aatgagaca 
        gggtatgac tacgagtgg ttccggcac atctccctt 
        accaaagat tttgaccaa ttggagcaa ctgtcaacc 
        tcatttaag gaccagatt aattcgata gcaaagttt 
        atgagtagt agcaacgag cctccacct ccaagcgag 
        ccaatctca actgaaatt ttatactcg gaggtgctc 
        caaggtcta tcgcccagt cgcaagctt ttatcttcg 
        ttcaaatgc gatcacaat aacaaggac gaaacgtcg 
        tcaactagc caggaaagc ttcacaaac tacacgatt 
        ttgaaccag aaagcgggg caagaagac gggaagcaa 
        gccaggcat tataagcca agtcaagtg gattcagtt 
        cagcgcaat acgcgggct agtgtccac ccttgcgtg 
        caacaagcg tatttttac tcgccggct gtccaacgg 
        tggagttat taccccacg caggttttt acgtccagt 
        tgggttgca caaagtccg gtggttaac cgacttttc 
        ggggcttca ccggttact atcgcctcc gaaagcgag 
        aaccaggtg gaaggggag gagcaccaa gtctcgacg 
        agtatttcc gaagagttg gaaatacaa gccttggag 
        cagtacagc aactcgaac gagaatttt taccaagag 
        ttggagagg caagcggct gagcagtac gcgaactcg 
        cccgaataa
      mRNA sequence
      • >TC009516 RA
        tgacatgcg tcattttcg tcaattaaa attttctca 
        aaaaatttg aatttcgtt taatccggt ctcaaaatt 
        ccgaagctt tgaaggtaa aaagccgcc aaattgtag 
        tacttgatc aaatatcgc gcaaaactg agtaacaaa 
        acacctgaa agctcgcgt acaagaagg cagtcgttg 
        ttgacgaaa aatcgaatc gcatttttc cttgacaac 
        tttcacatg ttgacatga acgtaaggg aacgtctga 
        aagaggcat atgagagcg tttctttca gtgccgata 
        gaacctggg caagcgtgc caacggcat tccacgcgt 
        gtaacttgg atttgcact tgactgaaa aggtcctgc 
        aaagtcaga agttgcatc gatttcggc ctcgcaccg 
        tcagagagg cgattccga agagcagat tggctcagt 
        atgtgaatt gcgtctaca atgagtcga gggaaatgt 
        catacgctg cgaacagtg cccttaatg aaacactcg 
        cacagagtg gagaaccga cggaagcct gggagatgc 
        ccgaagtga gggagctgt acaagtggt tcttgaaac 
        agcctgaag atgattttt acagagttg acagacggt 
        atgaatcgc cgttgctca acgtcaaag gcttcgaag 
        acaagattt tgaaaatac aaatcaccg aagacagag 
        ttaaaaata tctggaaca gcgattttt accaaatcg 
        gtgaacccc gcattaaaa acgagcaaa tcatagtta 
        atttcttta cgtccccga tgagcaacg tgtttcttt 
        tcgacaatg agacagggt atgactacg agtggttcc 
        ggcacatct cccttacca aagattttg accaattgg 
        agcaactgt caacctcat ttaaggacc agattaatt 
        cgatagcaa agtttatga gtagtagca acgagcctc 
        cacctccaa gcgagccaa tctcaactg aaattttat 
        actcggagg tgctccaag gtctatcgc ccagtcgca 
        agcttttat cttcgttca aatgcgatc acaataaca 
        aggacgaaa cgtcgtcaa ctagccagg aaagcttca 
        caaactaca cgattttga accagaaag cggggcaag 
        aagacggga agcaagcca ggcattata agccaagtc 
        aagtggatt cagttcagc gcaatacgc gggctagtg 
        tccaccctt gcgtgcaac aagcgtatt tttactcgc 
        cggctgtcc aacggtgga gttattacc ccacgcagg 
        tttttacgt ccagttggg ttgcacaaa gtccggtgg 
        ttaaccgac ttttcgggg cttcaccgg ttactatcg 
        cctccgaaa gcgagaacc aggtggaag gggaggagc 
        accaagtct cgacgagta tttccgaag agttggaaa 
        tacaagcct tggagcagt acagcaact cgaacgaga 
        atttttacc aagagttgg agaggcaag cggctgagc 
        agtacgcga actcgcccg aataatttt ttaacctaa 
        ttgttaagc aataaatag taggaagta aa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY