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Gene information
General information
TC009507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 148317..151930
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009507
    iBeetle screen iB_04646

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009507 RA
      MSKVHLLPR FGQIKSNFL KVHGRVLHV AQLSSSSDD 
      EKKVQKKDE DTKKSNEAL KKLNNLLKT MIEDDVQTS 
      DKKLNLAKP PNRRQGQQE KKKPVETPE TQMTRVVKD 
      VAETLGGDV KQTETELLM KLLKTSEEG KASNLVDLV 
      KGMKVDTEK SPSEQSKAQ QVRKILQAK TGEKPMFTR 
      RPPRSARPM RTELPTEKI DLFGAESLG IFTDKASYV 
      SEGVENKTW NELYQRDLK LAVTHPPSN YFQQMILWT 
      EQGKIWKFP IDNEQGLDE EKKVYFTEH VFLEKYLEP 
      WCPQKGPLR HFMELVCVG LSKNPYLTV DAKREHIEW 
      FKNYFEEKK QLLKEVGAL QDRELGNKN TPKQIEH
    CDS sequence
    • >TC009507 RA
      atgagtaaa gtgcacctt ttaccaagg tttggccaa 
      attaagagc aacttctta aaagtgcat ggaagagtt 
      ttgcacgta gcacagttg agtagcagt tcagacgat 
      gaaaagaaa gtccagaaa aaggatgag gacacaaag 
      aaaagtaac gaagccctc aaaaagtta aataacttg 
      ttaaagaca atgattgag gacgatgtg cagactagt 
      gataagaaa cttaatttg gcgaagcct cccaatagg 
      agacagggc cagcaagag aagaaaaaa ccggtggaa 
      actcctgag acacaaatg accagagtt gtaaaggac 
      gtagcagag acgttggga ggagatgtg aagcaaacc 
      gagacagaa ttattgatg aagctttta aagacgtct 
      gaagagggc aaagcgtct aatttagtt gatttggtt 
      aaaggaatg aaagttgat actgagaaa tcacctagt 
      gaacaatcc aaggcacaa caagttagg aaaattttg 
      caagcaaaa acgggggaa aaaccaatg tttacacga 
      aggccgccg aggagcgcc aggccaatg agaacagaa 
      ctaccaacc gagaaaatt gatctcttc ggagccgaa 
      tctttgggg attttcaca gataaagca agttacgtt 
      agtgagggc gttgaaaac aaaacttgg aacgagtta 
      taccagagg gatttaaag ctggctgtt actcaccct 
      cctagcaat tatttccaa cagatgatc ttgtggacc 
      gaacagggg aaaatatgg aaatttccc attgataac 
      gaacaaggg cttgatgag gaaaagaaa gtttacttt 
      accgagcac gtgtttctt gagaagtat ttagagcct 
      tggtgtcct caaaagggg cccttgagg cacttcatg 
      gagttggtc tgtgtcgga ctttcgaaa aatccatac 
      ttgacagtt gacgccaaa cgggaacat atcgagtgg 
      ttcaaaaac tattttgaa gagaagaaa caattattg 
      aaagaagtt ggggcacta caagacaga gagttaggt 
      aacaaaaac actcctaaa caaattgaa cactaa
    mRNA sequence
    • >TC009507 RA
      gatcaccaa agtggacta aaaataaac ttcttgcca 
      tacatgttc cgtccgttg ggttgaggg ggccataaa 
      gcgcagcac taattggaa attaattta caaagcatt 
      ccaccgaaa cagatgcaa caaataaat aattattta 
      attaatttc acaattttt tactgtcaa aagtgaact 
      tatcgaaat gtcaaaatg ggaagtgtt tatgattcc 
      aaaaagaat gaaaattaa ttaattatt acacaaaga 
      aaaaataaa ttataaaca tttacaggt gcatcgctg 
      tatccagtt catttaata caatattct tcttacaaa 
      aataatttt aaaaccttt cggaaaatg ggctaatga 
      cgtagccat agccatagc cacccaacc taacctaca 
      aaacctctg attattttt tgggttttt attgtgtga 
      aaatgagta aagtgcacc ttttaccaa ggtttggcc 
      aaattaaga gcaacttct taaaagtgc atggaagag 
      ttttgcacg tagcacagt tgagtagca gttcagacg 
      atgaaaaga aagtccaga aaaaggatg aggacacaa 
      agaaaagta acgaagccc tcaaaaagt taaataact 
      tgttaaaga caatgattg aggacgatg tgcagacta 
      gtgataaga aacttaatt tggcgaagc ctcccaata 
      ggagacagg gccagcaag agaagaaaa aaccggtgg 
      aaactcctg agacacaaa tgaccagag ttgtaaagg 
      acgtagcag agacgttgg gaggagatg tgaagcaaa 
      ccgagacag aattattga tgaagcttt taaagacgt 
      ctgaagagg gcaaagcgt ctaatttag ttgatttgg 
      ttaaaggaa tgaaagttg atactgaga aatcaccta 
      gtgaacaat ccaaggcac aacaagtta ggaaaattt 
      tgcaagcaa aaacggggg aaaaaccaa tgtttacac 
      gaaggccgc cgaggagcg ccaggccaa tgagaacag 
      aactaccaa ccgagaaaa ttgatctct tcggagccg 
      aatctttgg ggattttca cagataaag caagttacg 
      ttagtgagg gcgttgaaa acaaaactt ggaacgagt 
      tataccaga gggatttaa agctggctg ttactcacc 
      ctcctagca attatttcc aacagatga tcttgtgga 
      ccgaacagg ggaaaatat ggaaatttc ccattgata 
      acgaacaag ggcttgatg aggaaaaga aagtttact 
      ttaccgagc acgtgtttc ttgagaagt atttagagc 
      cttggtgtc ctcaaaagg ggcccttga ggcacttca 
      tggagttgg tctgtgtcg gactttcga aaaatccat 
      acttgacag ttgacgcca aacgggaac atatcgagt 
      ggttcaaaa actattttg aagagaaga aacaattat 
      tgaaagaag ttggggcac tacaagaca gagagttag 
      gtaacaaaa acactccta aacaaattg aacactaat 
      ttagtacaa caatagtaa atacagtat tggaaaatg 
      aaactttgt tttaatgac aaacctaca atttttaaa 
      gggaaattc agcatttat taaaactta tttcgacac 
      atgtaatgt aataaaaac aaaactcaa acgaatata 
      aaaagttga aaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY