Status
Please wait ...
Gene information
General information
TC009492 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 320125..324834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009492
    iBeetle screen iB_04645

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal partiallyorientation irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment posterior fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009492 RA
      MMEEMFTEL PVPLLSTAA IIRHHTRYQ PYQRLHQPQ 
      HFHTPVQEL HALQQPDHG TTHISCLYP EILAIIFSY 
      LEVRDKGRV AQVCTAWRD AAYNKSVWR GVEAKLHLR 
      RANPSLFAS LVKRGIKRV QVLSLRKSL RDVIQGIPN 
      LESLNLRGC YNVGDVGIS HAFVADSPT LTELDLSLC 
      KQVTDTSLT RIAQHLKNL EVLELGGCS NVTNSGLML 
      IAWGLKKLK RLNLRSCWH VGDQGIQHL ASGNPSLEH 
      LGLQDCQKL SDEALKHAT GLTSLISIN LSFCVSITD 
      SGLKHLAKM TNLRELNLR SCDNISDTG MAFLAEGGS 
      RISSLDVSF CDKIGDQAL VHISQGLFN LRNLLMSAC 
      QLSDEGLAK IANSLHDLE TLNIGQCSR VTDKGLTTI 
      AESLLRLKC IDLYGCTRI TTVGLERIM KLPQLSVLN 
      LGLWHVR
    CDS sequence
    • >TC009492 RA
      atgatggag gagatgttc actgagttg cccgtcccc 
      ttgctgtcc acagcggcc atcatccgc caccacacc 
      agataccag ccctaccaa aggctgcac cagccccag 
      cacttccac acgcccgtc caggagctc cacgcgctg 
      caacaacca gaccatggc accacgcac atctcctgt 
      ctctatcca gagatcttg gccatcatc ttcagctac 
      ctggaggtg cgcgacaag ggccgcgtg gcgcaggtt 
      tgcactgcg tggcgcgac gccgcttac aacaagtcc 
      gtgtggcgc ggcgtcgaa gccaaactc cacctccgt 
      cgcgctaac ccttcacta ttcgcttcg ctggtgaaa 
      cgcggtatc aaacgtgtt caggtcctg tccctacgt 
      aaatccctc cgggacgtc atccagggc atccctaac 
      ttggagtct ttgaacctc cgtggttgt tacaacgtg 
      ggggacgtg ggcatatcc cacgcattt gtggcagac 
      tcacccacc ctcaccgaa ctggacctc tccctctgc 
      aagcaagtc accgacacg tccctcaca cgcatcgcc 
      caacacttg aagaacttg gaggtgctg gaactgggc 
      ggttgctcc aacgtcacc aactccggc ctgatgctc 
      atcgcctgg ggcctcaag aagctcaaa agactgaat 
      ttacgctcg tgctggcac gtgggggac caaggaatc 
      caacacttg gcttcgggg aatccatcg ctcgagcac 
      ttggggctc caagactgt cagaaactc tccgacgag 
      gctctcaaa cacgccacc ggcttgact tccctcatc 
      tcaatcaat ctgagtttt tgtgttagt ataacggac 
      agcgggtta aaacaccta gccaaaatg acgaattta 
      cgcgagttg aatttgcgt tcgtgtgat aatatttcc 
      gacacggga atggcgttc cttgcagaa ggcgggtcg 
      agaatttcg tctttggac gtatcattt tgtgacaaa 
      atcggtgac caagcgcta gtgcacatt tcacaagga 
      ctgttcaat ttgcgaaat ttgttaatg agtgcgtgt 
      caactctcg gatgaaggt ctggcgaaa atcgcgaat 
      tcgctccat gacctggag acgctcaac atcggacag 
      tgcagtcgt gtgacggat aagggctta acaaccatt 
      gctgagtcg ctcctgaga ctaaagtgc atcgatttg 
      tacggatgc accaggata acgacggta ggacttgaa 
      cgaattatg aaattgccg cagttgagt gtcctcaat 
      ttggggctg tggcatgtt aggtga
    mRNA sequence
    • >TC009492 RA
      ggtcacgtg gtgcgcact cggaccaat ggcagcact 
      cgcaactcg tttcgtctg ttctccagt ttggtcgcc 
      aacagtttg aaatgagtc gttgtagag gtgagaagg 
      cgcccgtgc tccgagtcg tggtgcgca cgtagtggt 
      tccgactat gatggagga gatgttcac tgagttgcc 
      cgtcccctt gctgtccac agcggccat catccgcca 
      ccacaccag ataccagcc ctaccaaag gctgcacca 
      gccccagca cttccacac gcccgtcca ggagctcca 
      cgcgctgca acaaccaga ccatggcac cacgcacat 
      ctcctgtct ctatccaga gatcttggc catcatctt 
      cagctacct ggaggtgcg cgacaaggg ccgcgtggc 
      gcaggtttg cactgcgtg gcgcgacgc cgcttacaa 
      caagtccgt gtggcgcgg cgtcgaagc caaactcca 
      cctccgtcg cgctaaccc ttcactatt cgcttcgct 
      ggtgaaacg cggtatcaa acgtgttca ggtcctgtc 
      cctacgtaa atccctccg ggacgtcat ccagggcat 
      ccctaactt ggagtcttt gaacctccg tggttgtta 
      caacgtggg ggacgtggg catatccca cgcatttgt 
      ggcagactc acccaccct caccgaact ggacctctc 
      cctctgcaa gcaagtcac cgacacgtc cctcacacg 
      catcgccca acacttgaa gaacttgga ggtgctgga 
      actgggcgg ttgctccaa cgtcaccaa ctccggcct 
      gatgctcat cgcctgggg cctcaagaa gctcaaaag 
      actgaattt acgctcgtg ctggcacgt gggggacca 
      aggaatcca acacttggc ttcggggaa tccatcgct 
      cgagcactt ggggctcca agactgtca gaaactctc 
      cgacgaggc tctcaaaca cgccaccgg cttgacttc 
      cctcatctc aatcaatct gagtttttg tgttagtat 
      aacggacag cgggttaaa acacctagc caaaatgac 
      gaatttacg cgagttgaa tttgcgttc gtgtgataa 
      tatttccga cacgggaat ggcgttcct tgcagaagg 
      cgggtcgag aatttcgtc tttggacgt atcattttg 
      tgacaaaat cggtgacca agcgctagt gcacatttc 
      acaaggact gttcaattt gcgaaattt gttaatgag 
      tgcgtgtca actctcgga tgaaggtct ggcgaaaat 
      cgcgaattc gctccatga cctggagac gctcaacat 
      cggacagtg cagtcgtgt gacggataa gggcttaac 
      aaccattgc tgagtcgct cctgagact aaagtgcat 
      cgatttgta cggatgcac caggataac gacggtagg 
      acttgaacg aattatgaa attgccgca gttgagtgt 
      cctcaattt ggggctgtg gcatgttag gtgaaacgt 
      atttgttga aatttgcgc cctctgaat gaaacgagt 
      cgagatgac caaaaatgc attcattcc caaatctcg 
      accggttca agacggtgg cggttttat gctgtgata 
      gattttaaa cgaatgacg taaagttcg actaaagtg 
      aaattaaac gtaagcatc gtaaacaat gagaactga 
      ttttctatg gtggtgccg ccaaaatga agcacatgc 
      tagttacag ggaataatt cacatgaat tcgaccgaa 
      caacttttt tgatactag tttattaaa acgttggtc 
      tgtttactc tttgttatt tttctactt aaatgttag 
      cacttctaa gtcgctatc aactttttt tttaatgaa 
      aagctatgt ttttcacta cgtttttgg attatttga 
      gttatttta agggaattt ttatcagct gtccccata 
      tctaaattt aatcgtttt cgagatatt taagaattt 
      ttggaaatg tttgaatta caccgttca cttagatgt 
      tcacattca tagtaattt tatttaaag gaggatttt 
      attgcagac gagtgtaat taaacgaaa aattccaaa 
      tagtgaata tttgcttca ttttgtgca caccaccca 
      agatttgac actgctaca agccttgga atttttttc 
      ataccttca ctagcacgt agcaccaca aatttgcaa 
      tattataca tcgaattgt gattagttt gtttcgaaa 
      aatgtgata gcggttaaa caatcattt tcgaaaacg 
      ttaagtttg aatttcgcc tctttgtta gcatcaggt 
      ttttgttca atttttgct tgttccgtg ttttttttt 
      tgtggtgca gagacaatt tttcaaact gtaaaaatg 
      tacagtgat cattatagt atttgtaaa taaacgtaa 
      agttatcaa tatttgtta ctattacgt tatgttttt 
      ataaattaa acaaattta aacttaata aagttttta 
      tcaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY