| General information | |||
| TC009489 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 365964..379584 | |
| Sequence information | |||
| Transcripts / Proteins (6) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009489 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04644 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
Days post injection
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC009489 RA
MHFFKYHEL KFCRELFSI TDIKLESRA ETKSSLSVE SGSSGAGHS SHSRGLAWT PPASVEGST PTWTEGTPS FTESSSSGD MGCPTTPLR QSSNTSMKA GHHKQTVEH AINCLTSEM EQLKNRVDT DYK
- >TC009489 RB
MHFFKYHEL KFCRELFSI TDIKLESRA ETKSSLSVE SGSSGAGHS SHSRGLAWT PPASVEGST PTWTEGTPS FTESSSSGD MGCPTTPLR QSSNTSMKA GHHKQTVEH AINCLTSEM VSFKP
- >TC009489 RC
MHFFKYHEL KFCRELFSI TDIKLESRA ETKSSLSVE SGSSGAGHS SHSRGLAWT PPASVEGST PTWTEGTPS FTESSSSGD MGCPTTPLR QSSNTSMKA GHHKQTVEH AINCLTSEM THL
- >TC009489 RD
MASGDTIET IEVVEPSLT ISMNNAKDS GGLHDSLDE SEDKDSSLQ RALQDAGQK QSSNVAKTK AEREKERER KIGHRRVGV GGEITYKKI QTSQIMGSI QLGIQHAVG GLASKPERD LLMQDFMTV ETTNFPSEG SNHTPAHHF SEFKFKTYA PIAFRYFRD LFGIQPDDF LMSMCDSPL RELSNPGAS GSIFYLTSD DEFIIKTVQ HKEGEFLQK LLPGYYMNL NQNPRTLLP KFFGLYCYQ CNSKNVRLV IMNNLLPSS VVMHQKYDL KGSTYKRKA SKAESQKRS PTYKDLDFM EHHPEGILM EADTYNALV KTIQRDCRV LESFKIMDY SLLLTIHNL DQAQREKQE QRSRNNLEE VPEEGTLSD PSGALLYNE REREKEDRI GAAALNRSR SINRQRLVA HSTAMESIQ AESEPIDED EDVPPGGIP ARNAKGERL LLFIGIIDI LQSYRLKKK LEHTWKSMI HDGDTVSVH RPSFYAQRF QKFMAEKVF KKIPSPLKH SPSKRKSLS RPLRPHEES DNNTTVQAQ VNASQQNGS KTGVAPSCC STPPPTFDE VLEQRASAS LEGVPSSTP ARVRVGSHH SHHHVTVKN YKEDVVSIT DIKLESRAE TKSSLSVES GSSGAGHSS HSRGLAWTP PASVEGSTP TWTEGTPSF TESSSSGDM GCPTTPLRQ SSNTSMKAG HHKQTVEHA INCLTSEMV SFKP
- >TC009489 RE
MASGDTIET IEVVEPSLT ISMNNAKDS GGLHDSLDE SEDKDSSLQ RALQDAGQK QSSNVAKTK AEREKERER KIGHRRVGV GGEITYKKI QTSQIMGSI QLGIQHAVG GLASKPERD LLMQDFMTV ETTNFPSEG SNHTPAHHF SEFKFKTYA PIAFRYFRD LFGIQPDDF LMSMCDSPL RELSNPGAS GSIFYLTSD DEFIIKTVQ HKEGEFLQK LLPGYYMNL NQNPRTLLP KFFGLYCYQ CNSKNVRLV IMNNLLPSS VVMHQKYDL KGSTYKRKA SKAESQKRS PTYKDLDFM EHHPEGILM EADTYNALV KTIQRDCRV LESFKIMDY SLLLTIHNL DQAQREKQE QRSRNNLEE VPEEGTLSD PSGALLYNE REREKEDRI GAAALNRSR SINRQRLVA HSTAMESIQ AESEPIDED EDVPPGGIP ARNAKGERL LLFIGIIDI LQSYRLKKK LEHTWKSMI HDGDTVSVH RPSFYAQRF QKFMAEKVF KKIPSPLKH SPSKRKSLS RPLRPHEES DNNSVAPSC CSTPPPTFD EVLEQRASA SLEGVPSST PARVRVGSH HSHHHVTVK NYKEDVVSI TDIKLESRA ETKSSLSVE SGSSGAGHS SHSRGLAWT PPASVEGST PTWTEGTPS FTESSSSGD MGCPTTPLR QSSNTSMKA GHHKQTVEH AINCLTSEM VSFKP
- >TC009489 RF
MASGDTIET IEVVEPSLT ISMNNAKDS GGLHDSLDE SEDKDSSLQ RALQDAGQK QSSNVAKTK AEREKERER KIGHRRVGV GGEITYKKI QTSQIMGSI QLGIQHAVG GLASKPERD LLMQDFMTV ETTNFPSEG SNHTPAHHF SEFKFKTYA PIAFRYFRD LFGIQPDDF LMSMCDSPL RELSNPGAS GSIFYLTSD DEFIIKTVQ HKEGEFLQK LLPGYYMNL NQNPRTLLP KFFGLYCYQ CNSKNVRLV IMNNLLPSS VVMHQKYDL KGSTYKRKA SKAESQKRS PTYKDLDFM EHHPEGILM EADTYNALV KTIQRDCRV LESFKIMDY SLLLTIHNL DQAQREKQE QRSRNNLEE VPEEGTLSD PSGALLYNE REREKEDRI GAAALNRSR SINRQRLVA HSTAMESIQ AESEPIDED EDVPPGGIP ARNAKGERL LLFIGIIDI LQSYRLKKK LEHTWKSMI HDGDTVSVH RPSFYAQRF QKFMAEKVF KKIPSPLKH SPSKRKSLS RPLRPHEES DNNTTVQAQ VNASQQNGS KTVSPTETI PAKISPPAY PSSVGTPLT SPPASSYPA VLVGRSSTE SKPKVPPPV PPRGTPKVK KGGNVNGKG ATHCGFVVS KRSHANNTT LLFVDDSVH DARTHRRSR SDEYLRKSK GDSNERLWG NLNWNVGDV HFYGMDDYV KI
- >TC009489 RA
atgcatttt tttaaatat catgaacta aaattttgt cgcgaattg tttagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg gaacagctg aagaatcgc gtggacacc gattacaaa tga
- >TC009489 RB
atgcatttt tttaaatat catgaacta aaattttgt cgcgaattg tttagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg gtgagtttc aagccttaa
- >TC009489 RC
atgcatttt tttaaatat catgaacta aaattttgt cgcgaattg tttagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg acgcacctt taa
- >TC009489 RD
atggcttcg ggtgataca atagaaact atcgaagtt gttgaacct tctttaaca ataagtatg aacaatgca aaggattca ggaggacta cacgactcc ctggacgag agtgaagat aaggactct tcactacaa agagcgcta caggatgct ggtcaaaaa cagtcctca aatgtggcc aaaacgaaa gccgaaagg gagaaggaa agggaaagg aaaatcggc catcgaagg gtcggcgtt ggtggcgaa atcacctac aaaaaaatc cagactagt caaattatg ggttctata caactaggt attcaacat gccgttgga ggtctagca tcaaaacct gaacgtgat ttattaatg caagacttt atgactgtt gaaacgacg aattttcca agcgaaggc tccaatcac actccagct catcatttt tccgagttt aaattcaaa acttacgcc cctatagct tttagatat ttccgagat ttgttcgga atacaacct gacgatttt ttaatgtca atgtgtgat agtccctta agagagttg tccaatccg ggagctagc ggatcgatt ttttatttg actagtgat gatgaattt attattaag actgtacag cataaggag ggagagttt ttgcaaaaa ttgttaccc ggttattat atgaatttg aatcaaaac ccgcgcacg ttactaccg aaattcttc ggcttgtac tgttatcaa tgtaatagc aaaaatgta agactggta attatgaat aatctgtta cctagctca gtagtgatg catcaaaag tatgatctc aaaggaagc acttacaaa agaaaggcg tctaaagcg gagagtcaa aagcgttcc ccgacttac aaagatctg gattttatg gaacatcat cctgagggt attttaatg gaagctgat acgtataat gctttagtc aagacgatt caacgcgat tgtcgagtg ttggaaagt tttaaaatt atggattat agtttactt ttaacgatt cataacttg gaccaggct cagagggag aaacaagaa caaagatct cgtaataat ctggaagaa gtacctgaa gagggtact ttatctgat cccagtggt gccctactt tataacgaa agggaaagg gagaaggag gacaggatc ggagctgct gctttgaat aggagtaga tcaatcaat cgacaaaga ctggttgcg cattccact gccatggag agcattcaa gcggaatcg gagcctata gatgaggat gaggatgtt cctcctggg ggcatacct gcaagaaat gcgaaaggc gagcgtttg ctgctgttt atcggaata atcgacatc ttgcagagc tataggcta aagaagaag ttggaacac acttggaag tcgatgatt cacgatggg gatacggta tcggtgcat agacctagt ttttacgct caaaggttt caaaaattc atggctgag aaagtgttc aaaaagatc ccttctcca ttaaagcat tccccttct aaaagaaaa tctttgtca cgacctctt cgaccacac gaagaatca gataacaac actactgtg caggcgcag gtgaatgct agccaacag aacggaagc aaaaccggt gtagctcca agctgctgc agtactcct ccaccaacc ttcgacgaa gtcctcgaa cagagagcc tctgcatcc cttgaagga gttccttct tccacaccg gccagagtt agggtcggc tctcatcat tcgcaccat cacgtcacg gtcaaaaat tacaaagag gacgttgtc agtataacc gatataaaa ttggagtca cgtgctgag accaagtct tctttgagt gtggagtcg ggcagttcg ggggctggt cattcgagc cactctcgg gggctagca tggacgccc cccgcctct gtggagggc tccaccccc acgtggacc gaaggtacg ccttcattt actgaatca tcaagtagc ggcgatatg ggatgtcca acaacaccg ttgcgtcaa tcgtccaac acttccatg aaggcagga catcacaaa caaacggtg gagcatgcc atcaactgt ctcacctct gaaatggtg agtttcaag ccttaa
- >TC009489 RE
atggcttcg ggtgataca atagaaact atcgaagtt gttgaacct tctttaaca ataagtatg aacaatgca aaggattca ggaggacta cacgactcc ctggacgag agtgaagat aaggactct tcactacaa agagcgcta caggatgct ggtcaaaaa cagtcctca aatgtggcc aaaacgaaa gccgaaagg gagaaggaa agggaaagg aaaatcggc catcgaagg gtcggcgtt ggtggcgaa atcacctac aaaaaaatc cagactagt caaattatg ggttctata caactaggt attcaacat gccgttgga ggtctagca tcaaaacct gaacgtgat ttattaatg caagacttt atgactgtt gaaacgacg aattttcca agcgaaggc tccaatcac actccagct catcatttt tccgagttt aaattcaaa acttacgcc cctatagct tttagatat ttccgagat ttgttcgga atacaacct gacgatttt ttaatgtca atgtgtgat agtccctta agagagttg tccaatccg ggagctagc ggatcgatt ttttatttg actagtgat gatgaattt attattaag actgtacag cataaggag ggagagttt ttgcaaaaa ttgttaccc ggttattat atgaatttg aatcaaaac ccgcgcacg ttactaccg aaattcttc ggcttgtac tgttatcaa tgtaatagc aaaaatgta agactggta attatgaat aatctgtta cctagctca gtagtgatg catcaaaag tatgatctc aaaggaagc acttacaaa agaaaggcg tctaaagcg gagagtcaa aagcgttcc ccgacttac aaagatctg gattttatg gaacatcat cctgagggt attttaatg gaagctgat acgtataat gctttagtc aagacgatt caacgcgat tgtcgagtg ttggaaagt tttaaaatt atggattat agtttactt ttaacgatt cataacttg gaccaggct cagagggag aaacaagaa caaagatct cgtaataat ctggaagaa gtacctgaa gagggtact ttatctgat cccagtggt gccctactt tataacgaa agggaaagg gagaaggag gacaggatc ggagctgct gctttgaat aggagtaga tcaatcaat cgacaaaga ctggttgcg cattccact gccatggag agcattcaa gcggaatcg gagcctata gatgaggat gaggatgtt cctcctggg ggcatacct gcaagaaat gcgaaaggc gagcgtttg ctgctgttt atcggaata atcgacatc ttgcagagc tataggcta aagaagaag ttggaacac acttggaag tcgatgatt cacgatggg gatacggta tcggtgcat agacctagt ttttacgct caaaggttt caaaaattc atggctgag aaagtgttc aaaaagatc ccttctcca ttaaagcat tccccttct aaaagaaaa tctttgtca cgacctctt cgaccacac gaagaatca gataacaac agtgtagct ccaagctgc tgcagtact cctccacca accttcgac gaagtcctc gaacagaga gcctctgca tcccttgaa ggagttcct tcttccaca ccggccaga gttagggtc ggctctcat cattcgcac catcacgtc acggtcaaa aattacaaa gaggacgtt gtcagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg gtgagtttc aagccttaa
- >TC009489 RF
atggcttcg ggtgataca atagaaact atcgaagtt gttgaacct tctttaaca ataagtatg aacaatgca aaggattca ggaggacta cacgactcc ctggacgag agtgaagat aaggactct tcactacaa agagcgcta caggatgct ggtcaaaaa cagtcctca aatgtggcc aaaacgaaa gccgaaagg gagaaggaa agggaaagg aaaatcggc catcgaagg gtcggcgtt ggtggcgaa atcacctac aaaaaaatc cagactagt caaattatg ggttctata caactaggt attcaacat gccgttgga ggtctagca tcaaaacct gaacgtgat ttattaatg caagacttt atgactgtt gaaacgacg aattttcca agcgaaggc tccaatcac actccagct catcatttt tccgagttt aaattcaaa acttacgcc cctatagct tttagatat ttccgagat ttgttcgga atacaacct gacgatttt ttaatgtca atgtgtgat agtccctta agagagttg tccaatccg ggagctagc ggatcgatt ttttatttg actagtgat gatgaattt attattaag actgtacag cataaggag ggagagttt ttgcaaaaa ttgttaccc ggttattat atgaatttg aatcaaaac ccgcgcacg ttactaccg aaattcttc ggcttgtac tgttatcaa tgtaatagc aaaaatgta agactggta attatgaat aatctgtta cctagctca gtagtgatg catcaaaag tatgatctc aaaggaagc acttacaaa agaaaggcg tctaaagcg gagagtcaa aagcgttcc ccgacttac aaagatctg gattttatg gaacatcat cctgagggt attttaatg gaagctgat acgtataat gctttagtc aagacgatt caacgcgat tgtcgagtg ttggaaagt tttaaaatt atggattat agtttactt ttaacgatt cataacttg gaccaggct cagagggag aaacaagaa caaagatct cgtaataat ctggaagaa gtacctgaa gagggtact ttatctgat cccagtggt gccctactt tataacgaa agggaaagg gagaaggag gacaggatc ggagctgct gctttgaat aggagtaga tcaatcaat cgacaaaga ctggttgcg cattccact gccatggag agcattcaa gcggaatcg gagcctata gatgaggat gaggatgtt cctcctggg ggcatacct gcaagaaat gcgaaaggc gagcgtttg ctgctgttt atcggaata atcgacatc ttgcagagc tataggcta aagaagaag ttggaacac acttggaag tcgatgatt cacgatggg gatacggta tcggtgcat agacctagt ttttacgct caaaggttt caaaaattc atggctgag aaagtgttc aaaaagatc ccttctcca ttaaagcat tccccttct aaaagaaaa tctttgtca cgacctctt cgaccacac gaagaatca gataacaac actactgtg caggcgcag gtgaatgct agccaacag aacggaagc aaaaccgtg agtcctact gaaacgata cccgccaaa atatcacct cctgcctat ccttcttca gtgggaaca cctctcact tctcctccg gcctcctcg tatcctgct gttctcgta ggacggtca agtaccgaa agtaagcct aaagtgccc ccgccagtt cctccccga ggcactccc aaagtaaaa aaagggggc aacgttaat ggcaaaggt gcgacgcat tgtggtttt gtagtatcg aaacgatcg catgccaat aacaccacg cttctgttt gttgatgat tctgtgcat gatgccaga acgcatcgg agaagtcgt tcagatgaa tatttacgt aaaagtaaa ggcgacagt aatgaacgc ctttgggga aatttgaat tggaatgtt ggcgatgtt catttttac ggcatggac gattatgtt aaaatttga
- >TC009489 RA
atgtaccaa ccaatgcag gtgtagctc caagctgct gcagtactc ctccaccaa ccttcgacg aagtcctcg aacagagag cctctgcat cccttgaag gagttcctt cttccacac cggccagag ttagggtcg gctctcatc attcgcacc atcacgtca cggtcaaaa attacaaag aggacgttg tcaggttag tttttctca atgcatttt tttaaatat catgaacta aaattttgt cgcgaattg tttagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg gaacagctg aagaatcgc gtggacacc gattacaaa tgaaaatgt aggtgtaat agcagactg atcatccat tcatcacgt tttgtttgt ttgtattaa aaaaaacgc tttaaagga caagaatat tctaaatac gtagtggtg ttatagtgg tattgtcgg gatttctca ttggcaata aaataattc aatcaaaat cgtgcatat cactattac actggtgtt ttagtgtgt attacatta acttggttt ttatgaact aaccagctt tgtgtttaa acaagtgcc aattattgc gttatttat tgtagcgga tcgagcgaa ggtattttt ttatgcgtt ggcgaaaat ggtgatgaa agaagttta ataaaaacc aagttttac tcttaagtg tttcctact gctcgcagt attgaaatg taattacat tccattcgg tgcaattag gacaaattg taaaatatt cccgctgcg aaaaaacct atgtgtaaa ttgcaatac tgtggtttt gattcatat ctaaggccg tttgtgctt tattattta ttataatct tctcgtatt tattgattt gtaaaggaa gtgggttta ggtctttta ttttttaca ctgggtggg accgagttc ggatcaaag ccacacgtt ttagttcga tttttacgt atctgtgat attaattga ctcataatt ttaacgcga aacaataca aagttttat tatttttga tttaaatta attgcccac ccggtgtaa tgctccagt attaagtcc atcttttta tatttttat ttatcgtgt taatcaagt cgtgcacta ttttctgtg tattctgcc tgtaaacaa acagttaat aattgttgt gtacttaca tacgtatcg aactgacct tcaaaccgc tctgttgtc ggaccgatt tgttggtcg gtttgtaat tttttgcaa atgaaatat tgtgtatat ggctcaggt aattgtgca tatttgtaa atgttggta gggtagtag ctgattatg gtatttgag taataatga tcataatta cttgaatat acgctttgg aatttgttg gcagaaacg aatttttat attaaattg ttattaggt atattgtta gcgaaaatt ttaatactc attgtttta actgcttag tggaatttt gctttaaaa atataaata tttcaacat t
- >TC009489 RB
atgtaccaa ccaatgcag gtgtagctc caagctgct gcagtactc ctccaccaa ccttcgacg aagtcctcg aacagagag cctctgcat cccttgaag gagttcctt cttccacac cggccagag ttagggtcg gctctcatc attcgcacc atcacgtca cggtcaaaa attacaaag aggacgttg tcaggttag tttttctca atgcatttt tttaaatat catgaacta aaattttgt cgcgaattg tttagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg gtgagtttc aagccttaa acaacacaa ctaatgcat ttttcacac ccagtaatt aaacacgat gatttcttt ggttttgtc ctgagtgtg agttttgca ataacaatt cacttgtgc tttttcaat cactcaaac actttgaag gtttcaatt caaacagtt caaaccttc aaaggctcg caccacggg gttcaccat gtaaatcag ttgttatta cacagttta gcgcaaaac ctaaatcta ggccgaaac tgatcttat tttatcgtt aaacaacgg tatttaagt tacttttca catttaata aatcttgtc gcgaatttt tttcttttt tgggcgttt tttttaatc acactagaa tattttttt tataaaaat cttctccaa tgctttgcg ttacaaaat ttgcgtttt tagagtttc ggtttagtt tttgtttat cgaacacaa cttaatcag tttccgctc gtttttttt tctatcaat tgcccactt tttggaaaa cggaattgt ttactcgtc cgtgtacac agtttacgt ttctcgctt cctctcctc tatgctagg catgctttt tgtatttat ttctccgtt tataataaa tgacaaaag aaacttttg atttccaaa aaatttagt ttttagcta gtatttaag tcttttttt tgtgcatgc ggttactta atttaattt gtcttctgt gtacaccga cgttattga cgttgattt tgcggttgg ttcatgctt gctttggaa attccacca ggaacagct gaagaatcg cgtggacac cgattacaa atgaaaatg taggtgtaa tagcagact gatcatcca ttcatcacg ttttgtttg tttgtatta aaaaaaacg ctttaaagg acaagaata ttctaaata cgtagtggt gttatagtg gtattgtcg ggatttctc attggcaat aaaataatt caatcaaaa tcgtgcata tcactatta cactggtgt tttagtgtg tattacatt aacttggtt tttatgaac taaccagct ttgtgttta aacaagtgc caattattg cgttattta ttgtagcgg atcgagcga aggtatttt tttatgcgt tggcgaaaa tggtgatga aagaagttt aataaaaac caagtttta ctcttaagt gtttcctac tgctcgcag tattgaaat gtaattaca ttccattcg gtgcaatta ggacaaatt gtaaaatat tcccgctgc gaaaaaacc tatgtgtaa attgcaata ctgtggttt tgattcata tctaaggcc gtttgtgct ttattattt attataatc ttctcgtat ttattgatt tgtaaagga agtgggttt aggtctttt attttttac actgggtgg gaccgagtt cggatcaaa gccacacgt tttagttcg atttttacg tatctgtga tattaattg actcataat tttaacgcg aaacaatac aaagtttta ttatttttg atttaaatt aattgccca cccggtgta atgctccag tattaagtc catcttttt atattttta tttatcgtg ttaatcaag tcgtgcact attttctgt gtattctgc ctgtaaaca aacagttaa taattgttg tgtacttac atacgtatc gaactgacc ttcaaaccg ctctgttgt cggaccgat ttgttggtc ggtttgtaa ttttttgca aatgaaata ttgtgtata tggctcagg taattgtgc atatttgta aatgttggt agggtagta gctgattat ggtatttga gtaataatg atcataatt acttgaata tacgctttg gaatttgtt ggcagaaac gaattttta tattaaatt gttattagg tatattgtt agcgaaaat tttaatact cattgtttt aactgctta gtggaattt tgctttaaa aatataaat atttcaaca tt
- >TC009489 RC
atgtaccaa ccaatgcag gtgtagctc caagctgct gcagtactc ctccaccaa ccttcgacg aagtcctcg aacagagag cctctgcat cccttgaag gagttcctt cttccacac cggccagag ttagggtcg gctctcatc attcgcacc atcacgtca cggtcaaaa attacaaag aggacgttg tcaggttag tttttctca atgcatttt tttaaatat catgaacta aaattttgt cgcgaattg tttagtata accgatata aaattggag tcacgtgct gagaccaag tcttctttg agtgtggag tcgggcagt tcgggggct ggtcattcg agccactct cgggggcta gcatggacg ccccccgcc tctgtggag ggctccacc cccacgtgg accgaaggt acgccttca tttactgaa tcatcaagt agcggcgat atgggatgt ccaacaaca ccgttgcgt caatcgtcc aacacttcc atgaaggca ggacatcac aaacaaacg gtggagcat gccatcaac tgtctcacc tctgaaatg acgcacctt taaggagca agaaacgat aacaagcca agttactgc caaacatta ttccaaata tttctcagg aatgtggta agagtgtca gtggaataa aattaggcc aaaggagat aagacccaa actgcgttc acctatatt atgttataa gtgtataat gtagtttta ggcagaata gagcaggaa aaatttaca agaaactta cttattgcc gaagcaata attcaagtt gcatttaaa ccaaatatt gcaagttta ttatttcat attttgtta ttaaattgt ttaataatt ttatcaact tttccttgt agattagat tttattttg aaattatca cgggaataa atattgctc atttccaca ttattggta cttaacgtg ctacactca cacacaaac taagtttgt tgcgctctc tgttgccaa aaaaaatat tagtaatta cgctatatc gtaataaaa agtcatttt tcgtaataa tactaataa acatgacgg taagttaaa cttgtcatt gtcattaca attcataaa aatgtcaac atcacattt ttcagaact gtagaaaaa atggcaaca gcgcattaa ttttttttg gggtcaaac gaatcacca attaaaata gtctgtgtt tccgtgtga ttttgtgtt aattggatt ttagtgaca aatttacag ccaaggaaa ttcaccaat tataagagt aaaccaatt gtgaaaagt tatttttat tattgtttt tcaacggtt taaaatcgg ctattcgag ggcgcaaaa atcttagct tgaaattat aattttact ttagccatg ctcatgata agaactcaa cacctgtta tatcggtaa aatcctaat tttattatt tttgtcaaa aatatgtca aatttgaat tttacagtc ccctagttc tgtcttaat ttgcatcaa tttttctaa aacctatac aataaataa agaaaatgc cgttaaaag cgacaatat gatggaaaa actgacatt tttttctgg aattgtccg taaaatagt acttttcag cacacttta tctgtcaag tcgtgtcaa aaatgacta aaaaagtgc atcgaatgc ataatattt aaaaatgca atgtgctaa aattacttt ttattacac tgagtgcgg ctaaagtag tacaaggtg tgttaaaat gattttcat ctagtcgct atgaatttc tcatactga cggacatta ataataaaa aaggtgttt tcttgcaca atatttact tgcttctta accaaacat ttgaatgca attaattga ctgttatga cgttaaatt catttctat ctagatgag aatcatttt aacacactt tgtatttta cgagtagga atatttaag tcataatat tattgtgat gtaaaaaat gtaaaatta tttattttc actattacg tatagataa ttttattga agttaattt agtacaaag aggtaacat tccttattg tgtaatttt tgatctaat aaatgaacc tcaattac
- >TC009489 RD
cgggtgggg gataaatat gtcaatcat gtcactgga atatgttta tgtcaaagt tgtaaatgg caactggtt tcattccca aaatgtgtg ctaaaagtt taataattt tgtgtaatt tgttagtga tgtgttgtg gctttgtaa atattaagt gaaaatagt cccgggtca gtttaaata agtgagcaa aatggcttc gggtgatac aatagaaac tatcgaagt tgttgaacc ttctttaac aataagtat gaacaatgc aaaggattc aggaggact acacgactc cctggacga gagtgaaga taaggactc ttcactaca aagagcgct acaggatgc tggtcaaaa acagtcctc aaatgtggc caaaacgaa agccgaaag ggagaagga aagggaaag gaaaatcgg ccatcgaag ggtcggcgt tggtggcga aatcaccta caaaaaaat ccagactag tcaaattat gggttctat acaactagg tattcaaca tgccgttgg aggtctagc atcaaaacc tgaacgtga tttattaat gcaagactt tatgactgt tgaaacgac gaattttcc aagcgaagg ctccaatca cactccagc tcatcattt ttccgagtt taaattcaa aacttacgc ccctatagc ttttagata tttccgaga tttgttcgg aatacaacc tgacgattt tttaatgtc aatgtgtga tagtccctt aagagagtt gtccaatcc gggagctag cggatcgat tttttattt gactagtga tgatgaatt tattattaa gactgtaca gcataagga gggagagtt tttgcaaaa attgttacc cggttatta tatgaattt gaatcaaaa cccgcgcac gttactacc gaaattctt cggcttgta ctgttatca atgtaatag caaaaatgt aagactggt aattatgaa taatctgtt acctagctc agtagtgat gcatcaaaa gtatgatct caaaggaag cacttacaa aagaaaggc gtctaaagc ggagagtca aaagcgttc cccgactta caaagatct ggattttat ggaacatca tcctgaggg tattttaat ggaagctga tacgtataa tgctttagt caagacgat tcaacgcga ttgtcgagt gttggaaag ttttaaaat tatggatta tagtttact tttaacgat tcataactt ggaccaggc tcagaggga gaaacaaga acaaagatc tcgtaataa tctggaaga agtacctga agagggtac tttatctga tcccagtgg tgccctact ttataacga aagggaaag ggagaagga ggacaggat cggagctgc tgctttgaa taggagtag atcaatcaa tcgacaaag actggttgc gcattccac tgccatgga gagcattca agcggaatc ggagcctat agatgagga tgaggatgt tcctcctgg gggcatacc tgcaagaaa tgcgaaagg cgagcgttt gctgctgtt tatcggaat aatcgacat cttgcagag ctataggct aaagaagaa gttggaaca cacttggaa gtcgatgat tcacgatgg ggatacggt atcggtgca tagacctag tttttacgc tcaaaggtt tcaaaaatt catggctga gaaagtgtt caaaaagat cccttctcc attaaagca ttccccttc taaaagaaa atctttgtc acgacctct tcgaccaca cgaagaatc agataacaa cactactgt gcaggcgca ggtgaatgc tagccaaca gaacggaag caaaaccgg tgtagctcc aagctgctg cagtactcc tccaccaac cttcgacga agtcctcga acagagagc ctctgcatc ccttgaagg agttccttc ttccacacc ggccagagt tagggtcgg ctctcatca ttcgcacca tcacgtcac ggtcaaaaa ttacaaaga ggacgttgt cagtataac cgatataaa attggagtc acgtgctga gaccaagtc ttctttgag tgtggagtc gggcagttc gggggctgg tcattcgag ccactctcg ggggctagc atggacgcc ccccgcctc tgtggaggg ctccacccc cacgtggac cgaaggtac gccttcatt tactgaatc atcaagtag cggcgatat gggatgtcc aacaacacc gttgcgtca atcgtccaa cacttccat gaaggcagg acatcacaa acaaacggt ggagcatgc catcaactg tctcacctc tgaaatggt gagtttcaa gccttaaac aacacaact aatgcattt ttcacaccc agtaattaa acacgatga tttctttgg ttttgtcct gagtgtgag ttttgcaat aacaattca cttgtgctt tttcaatca ctcaaacac tttgaaggt ttcaattca aacagttca aaccttcaa aggctcgca ccacggggt tcaccatgt aaatcagtt gttattaca cagtttagc gcaaaacct aaatctagg ccgaaactg atcttattt tatcgttaa acaacggta tttaagtta cttttcaca tttaataaa tcttgtcgc gaatttttt tcttttttg ggcgttttt tttaatcac actagaata tttttttta taaaaatct tctccaatg ctttgcgtt acaaaattt gcgttttta gagtttcgg tttagtttt tgtttatcg aacacaact taatcagtt tccgctcgt ttttttttc tatcaattg cccactttt tggaaaacg gaattgttt actcgtccg tgtacacag tttacgttt ctcgcttcc tctcctcta tgctaggca tgctttttg tatttattt ctccgttta taataaatg acaaaagaa acttttgat ttccaaaaa atttagttt ttagctagt atttaagtc ttttttttg tgcatgcgg ttacttaat ttaatttgt cttctgtgt acaccgacg ttattgacg ttgattttg cggttggtt catgcttgc tttggaaat tccaccagg aacagctga agaatcgcg tggacaccg attacaaat gaaaatgta ggtgtaata gcagactga tcatccatt catcacgtt ttgtttgtt tgtattaaa aaaaacgct ttaaaggac aagaatatt ctaaatacg tagtggtgt tatagtggt attgtcggg atttctcat tggcaataa aataattca atcaaaatc gtgcatatc actattaca ctggtgttt tagtgtgta ttacattaa cttggtttt tatgaacta accagcttt gtgtttaaa caagtgcca attattgcg ttatttatt gtagcggat cgagcgaag gtatttttt tatgcgttg gcgaaaatg gtgatgaaa gaagtttaa taaaaacca agttttact cttaagtgt ttcctactg ctcgcagta ttgaaatgt aattacatt ccattcggt gcaattagg acaaattgt aaaatattc ccgctgcga aaaaaccta tgtgtaaat tgcaatact gtggttttg attcatatc taaggccgt ttgtgcttt attatttat tataatctt ctcgtattt attgatttg taaaggaag tgggtttag gtcttttat tttttacac tgggtggga ccgagttcg gatcaaagc cacacgttt tagttcgat ttttacgta tctgtgata ttaattgac tcataattt taacgcgaa acaatacaa agttttatt atttttgat ttaaattaa ttgcccacc cggtgtaat gctccagta ttaagtcca tctttttat atttttatt tatcgtgtt aatcaagtc gtgcactat tttctgtgt attctgcct gtaaacaaa cagttaata attgttgtg tacttacat acgtatcga actgacctt caaaccgct ctgttgtcg gaccgattt gttggtcgg tttgtaatt ttttgcaaa tgaaatatt gtgtatatg gctcaggta attgtgcat atttgtaaa tgttggtag ggtagtagc tgattatgg tatttgagt aataatgat cataattac ttgaatata cgctttgga atttgttgg cagaaacga atttttata ttaaattgt tattaggta tattgttag cgaaaattt taatactca ttgttttaa ctgcttagt ggaattttg ctttaaaaa tataaatat ttcaacatt
- >TC009489 RE
cgggtgggg gataaatat gtcaatcat gtcactgga atatgttta tgtcaaagt tgtaaatgg caactggtt tcattccca aaatgtgtg ctaaaagtt taataattt tgtgtaatt tgttagtga tgtgttgtg gctttgtaa atattaagt gaaaatagt cccgggtca gtttaaata agtgagcaa aatggcttc gggtgatac aatagaaac tatcgaagt tgttgaacc ttctttaac aataagtat gaacaatgc aaaggattc aggaggact acacgactc cctggacga gagtgaaga taaggactc ttcactaca aagagcgct acaggatgc tggtcaaaa acagtcctc aaatgtggc caaaacgaa agccgaaag ggagaagga aagggaaag gaaaatcgg ccatcgaag ggtcggcgt tggtggcga aatcaccta caaaaaaat ccagactag tcaaattat gggttctat acaactagg tattcaaca tgccgttgg aggtctagc atcaaaacc tgaacgtga tttattaat gcaagactt tatgactgt tgaaacgac gaattttcc aagcgaagg ctccaatca cactccagc tcatcattt ttccgagtt taaattcaa aacttacgc ccctatagc ttttagata tttccgaga tttgttcgg aatacaacc tgacgattt tttaatgtc aatgtgtga tagtccctt aagagagtt gtccaatcc gggagctag cggatcgat tttttattt gactagtga tgatgaatt tattattaa gactgtaca gcataagga gggagagtt tttgcaaaa attgttacc cggttatta tatgaattt gaatcaaaa cccgcgcac gttactacc gaaattctt cggcttgta ctgttatca atgtaatag caaaaatgt aagactggt aattatgaa taatctgtt acctagctc agtagtgat gcatcaaaa gtatgatct caaaggaag cacttacaa aagaaaggc gtctaaagc ggagagtca aaagcgttc cccgactta caaagatct ggattttat ggaacatca tcctgaggg tattttaat ggaagctga tacgtataa tgctttagt caagacgat tcaacgcga ttgtcgagt gttggaaag ttttaaaat tatggatta tagtttact tttaacgat tcataactt ggaccaggc tcagaggga gaaacaaga acaaagatc tcgtaataa tctggaaga agtacctga agagggtac tttatctga tcccagtgg tgccctact ttataacga aagggaaag ggagaagga ggacaggat cggagctgc tgctttgaa taggagtag atcaatcaa tcgacaaag actggttgc gcattccac tgccatgga gagcattca agcggaatc ggagcctat agatgagga tgaggatgt tcctcctgg gggcatacc tgcaagaaa tgcgaaagg cgagcgttt gctgctgtt tatcggaat aatcgacat cttgcagag ctataggct aaagaagaa gttggaaca cacttggaa gtcgatgat tcacgatgg ggatacggt atcggtgca tagacctag tttttacgc tcaaaggtt tcaaaaatt catggctga gaaagtgtt caaaaagat cccttctcc attaaagca ttccccttc taaaagaaa atctttgtc acgacctct tcgaccaca cgaagaatc agataacaa cagtgtagc tccaagctg ctgcagtac tcctccacc aaccttcga cgaagtcct cgaacagag agcctctgc atcccttga aggagttcc ttcttccac accggccag agttagggt cggctctca tcattcgca ccatcacgt cacggtcaa aaattacaa agaggacgt tgtcagtat aaccgatat aaaattgga gtcacgtgc tgagaccaa gtcttcttt gagtgtgga gtcgggcag ttcgggggc tggtcattc gagccactc tcgggggct agcatggac gccccccgc ctctgtgga gggctccac ccccacgtg gaccgaagg tacgccttc atttactga atcatcaag tagcggcga tatgggatg tccaacaac accgttgcg tcaatcgtc caacacttc catgaaggc aggacatca caaacaaac ggtggagca tgccatcaa ctgtctcac ctctgaaat ggtgagttt caagcctta aacaacaca actaatgca tttttcaca cccagtaat taaacacga tgatttctt tggttttgt cctgagtgt gagttttgc aataacaat tcacttgtg ctttttcaa tcactcaaa cactttgaa ggtttcaat tcaaacagt tcaaacctt caaaggctc gcaccacgg ggttcacca tgtaaatca gttgttatt acacagttt agcgcaaaa cctaaatct aggccgaaa ctgatctta ttttatcgt taaacaacg gtatttaag ttacttttc acatttaat aaatcttgt cgcgaattt ttttctttt ttgggcgtt ttttttaat cacactaga atatttttt ttataaaaa tcttctcca atgctttgc gttacaaaa tttgcgttt ttagagttt cggtttagt ttttgttta tcgaacaca acttaatca gtttccgct cgttttttt ttctatcaa ttgcccact ttttggaaa acggaattg tttactcgt ccgtgtaca cagtttacg tttctcgct tcctctcct ctatgctag gcatgcttt ttgtattta tttctccgt ttataataa atgacaaaa gaaactttt gatttccaa aaaatttag tttttagct agtatttaa gtctttttt ttgtgcatg cggttactt aatttaatt tgtcttctg tgtacaccg acgttattg acgttgatt ttgcggttg gttcatgct tgctttgga aattccacc aggaacagc tgaagaatc gcgtggaca ccgattaca aatgaaaat gtaggtgta atagcagac tgatcatcc attcatcac gttttgttt gtttgtatt aaaaaaaac gctttaaag gacaagaat attctaaat acgtagtgg tgttatagt ggtattgtc gggatttct cattggcaa taaaataat tcaatcaaa atcgtgcat atcactatt acactggtg ttttagtgt gtattacat taacttggt ttttatgaa ctaaccagc tttgtgttt aaacaagtg ccaattatt gcgttattt attgtagcg gatcgagcg aaggtattt ttttatgcg ttggcgaaa atggtgatg aaagaagtt taataaaaa ccaagtttt actcttaag tgtttccta ctgctcgca gtattgaaa tgtaattac attccattc ggtgcaatt aggacaaat tgtaaaata ttcccgctg cgaaaaaac ctatgtgta aattgcaat actgtggtt ttgattcat atctaaggc cgtttgtgc tttattatt tattataat cttctcgta tttattgat ttgtaaagg aagtgggtt taggtcttt tatttttta cactgggtg ggaccgagt tcggatcaa agccacacg ttttagttc gatttttac gtatctgtg atattaatt gactcataa ttttaacgc gaaacaata caaagtttt attattttt gatttaaat taattgccc acccggtgt aatgctcca gtattaagt ccatctttt tatattttt atttatcgt gttaatcaa gtcgtgcac tattttctg tgtattctg cctgtaaac aaacagtta ataattgtt gtgtactta catacgtat cgaactgac cttcaaacc gctctgttg tcggaccga tttgttggt cggtttgta attttttgc aaatgaaat attgtgtat atggctcag gtaattgtg catatttgt aaatgttgg tagggtagt agctgatta tggtatttg agtaataat gatcataat tacttgaat atacgcttt ggaatttgt tggcagaaa cgaattttt atattaaat tgttattag gtatattgt tagcgaaaa ttttaatac tcattgttt taactgctt agtggaatt ttgctttaa aaatataaa tatttcaac att
- >TC009489 RF
cgggtgggg gataaatat gtcaatcat gtcactgga atatgttta tgtcaaagt tgtaaatgg caactggtt tcattccca aaatgtgtg ctaaaagtt taataattt tgtgtaatt tgttagtga tgtgttgtg gctttgtaa atattaagt gaaaatagt cccgggtca gtttaaata agtgagcaa aatggcttc gggtgatac aatagaaac tatcgaagt tgttgaacc ttctttaac aataagtat gaacaatgc aaaggattc aggaggact acacgactc cctggacga gagtgaaga taaggactc ttcactaca aagagcgct acaggatgc tggtcaaaa acagtcctc aaatgtggc caaaacgaa agccgaaag ggagaagga aagggaaag gaaaatcgg ccatcgaag ggtcggcgt tggtggcga aatcaccta caaaaaaat ccagactag tcaaattat gggttctat acaactagg tattcaaca tgccgttgg aggtctagc atcaaaacc tgaacgtga tttattaat gcaagactt tatgactgt tgaaacgac gaattttcc aagcgaagg ctccaatca cactccagc tcatcattt ttccgagtt taaattcaa aacttacgc ccctatagc ttttagata tttccgaga tttgttcgg aatacaacc tgacgattt tttaatgtc aatgtgtga tagtccctt aagagagtt gtccaatcc gggagctag cggatcgat tttttattt gactagtga tgatgaatt tattattaa gactgtaca gcataagga gggagagtt tttgcaaaa attgttacc cggttatta tatgaattt gaatcaaaa cccgcgcac gttactacc gaaattctt cggcttgta ctgttatca atgtaatag caaaaatgt aagactggt aattatgaa taatctgtt acctagctc agtagtgat gcatcaaaa gtatgatct caaaggaag cacttacaa aagaaaggc gtctaaagc ggagagtca aaagcgttc cccgactta caaagatct ggattttat ggaacatca tcctgaggg tattttaat ggaagctga tacgtataa tgctttagt caagacgat tcaacgcga ttgtcgagt gttggaaag ttttaaaat tatggatta tagtttact tttaacgat tcataactt ggaccaggc tcagaggga gaaacaaga acaaagatc tcgtaataa tctggaaga agtacctga agagggtac tttatctga tcccagtgg tgccctact ttataacga aagggaaag ggagaagga ggacaggat cggagctgc tgctttgaa taggagtag atcaatcaa tcgacaaag actggttgc gcattccac tgccatgga gagcattca agcggaatc ggagcctat agatgagga tgaggatgt tcctcctgg gggcatacc tgcaagaaa tgcgaaagg cgagcgttt gctgctgtt tatcggaat aatcgacat cttgcagag ctataggct aaagaagaa gttggaaca cacttggaa gtcgatgat tcacgatgg ggatacggt atcggtgca tagacctag tttttacgc tcaaaggtt tcaaaaatt catggctga gaaagtgtt caaaaagat cccttctcc attaaagca ttccccttc taaaagaaa atctttgtc acgacctct tcgaccaca cgaagaatc agataacaa cactactgt gcaggcgca ggtgaatgc tagccaaca gaacggaag caaaaccgt gagtcctac tgaaacgat acccgccaa aatatcacc tcctgccta tccttcttc agtgggaac acctctcac ttctcctcc ggcctcctc gtatcctgc tgttctcgt aggacggtc aagtaccga aagtaagcc taaagtgcc cccgccagt tcctccccg aggcactcc caaagtaaa aaaaggggg caacgttaa tggcaaagg tgcgacgca ttgtggttt tgtagtatc gaaacgatc gcatgccaa taacaccac gcttctgtt tgttgatga ttctgtgca tgatgccag aacgcatcg gagaagtcg ttcagatga atatttacg taaaagtaa aggcgacag taatgaacg cctttgggg aaatttgaa ttggaatgt tggcgatgt tcattttta cggcatgga cgattatgt taaaatttg agtgtgtgt tttcacagt ttttgcaca gtttcgaaa tctataaaa ttcaaatgc gacgttggc gtttttaca gttttgaaa ctgctaata taatgggta aaatgat
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