Status
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Gene information
General information
TC009474 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 580859..584243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009474
    iBeetle screen iB_04639

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009474 RA
      MVVEPLEEK VIARIRSSS SIDSVTQCI TELVLNSLD 
      AKATAIAVR VNFTTFRLQ VVDNGRGIS RPNLEFVGQ 
      RYMTNKCHS LDDLHKHLK FYGFKGEAL SNISHISQR 
      VTVETREHN SEETFVKIL EKDKQTINS SKCRASHGT 
      TVTVEGFLH NLPVRQQCV KNNELENIK RSLECLIII 
      HPRVSFTLR NDLGLKVVL SSVATPDIA SSFKTIHPE 
      IDENEFALL KVGKNKTRV EGLIYKQLH ENKRLQYLY 
      VNKRPVNSP KILNFVNAL FKTVPKTDP KRKNPVFVI 
      HIKCPYSDI DITLEPAKT LVFFRKIDL VKRCLQKMI 
      STFKGGDSE IVAKKTHRP LSEFGVSQI GGAVKGFGT 
      KRKSEDLEE LVSNKSAKI VEDTPYFEE PRVVSKQTK 
      VALPIEVKK GEKRESEGI MDNSLYTNY PDNEKKGKD 
      VIMDMFIMS LDVFPPEDK ANETEPELN NVVDMETTL 
      DKSPNEKNA LSQKSPIKE NMVSVGIQA GPGNIPVFG 
      VTPRKAMSK YVSDYDFDF SNIDALPTF HFKTPELPT 
      HYNQPKKLF DFRNTRRSS FNIFANENT FDQVTQSPY 
      FAKKAKASK FEIDSNKEN WSNDLDNTV DFKFIKKDN 
      TKRILNESH TSKYFTPQK SKSCKLRVQ TPKHTLLEE 
      SRDLFDKSS ESGLLITLD EEIEKNKTE DIVIAPTFT 
      QHQNPWLRK TGNDITFEM TERFDFVPK GLSPILKDC 
      GKVDDMSPK SRQQLQNAL VQSYEDELL MVKWQKCLD 
      DGDPKKFFD EIYVEKSRL IESEVPNAY NRHVKDFEN 
      ISFTKDLFK NVDIIGQVD CKFLAVWER TRNLVVLFD 
      QHAVHERVR LEELSEGYK NASTKLDQD ITIFLPQSD 
      LTLLKRQKT NLKSLGLVL EFFTNGLTI YQVPLCLYN 
      KSKKGVDIV FLTQTLIKE IVDLLKTNN EIMVNTPSL 
      IQNIVNSEA CRGAIKFGD SLTKNECFA HLKNLAKCK 
      LPFQCAHGR PTLTPLIFL NRFYDEEVA KPNLHNLRE 
      N
    CDS sequence
    • >TC009474 RA
      atggtggtc gaaccactt gaagaaaaa gtgattgcg 
      cgaattcga agctcctct tcaattgat agcgtgact 
      cagtgcatc acagaactg gttttgaac agtttggac 
      gcgaaagcg acagctatt gctgttcga gtcaatttt 
      acaactttt cgtctacaa gttgtagac aatgggcgt 
      ggaatttcg cgcccgaat ttagaattt gtgggtcaa 
      cgctacatg accaacaaa tgccacagc ttggacgac 
      ctccacaaa cacctaaaa ttctacggt ttcaaaggc 
      gaagccttg tctaacatt tcgcacatt tcccaacgt 
      gtgacagtt gagactagg gaacacaac agtgaggag 
      actttcgtc aaaatcttg gaaaaagat aaacaaaca 
      atcaattca agtaaatgt cgggccagc catggcacc 
      actgtcaca gttgagggt tttttgcac aacttacca 
      gtgagacaa caatgcgtc aaaaacaac gaattggaa 
      aatatcaag cggagcctt gagtgcctt attataatc 
      cacccgcgc gtgtcgttc actttgcga aacgattta 
      ggcctgaaa gttgttctt tcaagcgtt gcaacacca 
      gatattgca agctccttc aaaacaatc caccctgaa 
      atagacgaa aacgaattc gctttgctc aaagttggc 
      aaaaataag acacgcgtt gaaggtctc atctacaaa 
      cagttgcac gaaaacaag cgcctgcag tacttgtat 
      gtcaataag agaccggtg aactcgccc aaaatcctc 
      aattttgtc aatgcgctt tttaaaacc gtccccaaa 
      actgatccg aagaggaag aaccccgtt tttgtcata 
      cacatcaag tgtccctac tccgacatt gatatcact 
      ctagaacca gcaaagacg cttgtgttc ttcagaaaa 
      attgatctt gtgaagcga tgtctccaa aaaatgatc 
      agtacattc aaagggggt gattccgaa atagtggcc 
      aagaaaacg cacaggcct ttgagtgag tttggcgtg 
      tcccagata ggaggggct gtgaaaggc ttcgggacg 
      aagcgcaaa agtgaggat ttggaagag ttggtgtcg 
      aataaatcg gcgaaaatt gtcgaggat acgccctat 
      tttgaggaa ccgagggtt gtgtccaag caaacgaag 
      gtggctttg cccattgag gtgaaaaag ggggagaaa 
      agggagagc gaaggtata atggataat agcctctat 
      accaattac cctgataac gaaaagaag gggaaagac 
      gtgattatg gatatgttt ataatgtca ctggacgtg 
      ttcccgcct gaagataag gctaatgaa actgaaccc 
      gaactaaat aatgtagtg gatatggaa actacttta 
      gataaatca ccaaatgaa aaaaatgct ctttcccaa 
      aaatctccg attaaggag aatatggtt tcagtgggt 
      attcaggca ggaccgggg aacataccg gtatttggg 
      gtcacacca cgaaaagct atgtctaaa tatgtgtct 
      gactatgat tttgatttt tccaacatc gatgcttta 
      ccgactttt cattttaaa acgccagaa ttgcccaca 
      cattacaat cagccgaag aaattgttc gattttcgt 
      aatacaagg cgtagcagt tttaatatt tttgctaat 
      gaaaatacg tttgatcaa gtgacgcaa tcgccgtat 
      tttgctaag aaggccaaa gctagcaaa tttgagatt 
      gattcgaat aaagagaac tggtcaaat gacttggat 
      aatacagtt gattttaag tttattaaa aaagacaat 
      acgaaaaga attttgaac gaatcacac actagtaaa 
      tacttcacg cctcaaaaa tcaaagagt tgtaaatta 
      agggttcaa accccgaaa cacaccctt ttggaagag 
      tcgcgagat ttatttgat aagtcatcc gaaagtggg 
      cttttaata accctggat gaggaaatt gagaaaaac 
      aaaactgag gatattgtc atagccccc acatttacg 
      caacatcag aacccttgg ttaaggaaa actggcaac 
      gacattact ttcgaaatg accgaacgc tttgacttt 
      gtccctaaa ggcctgtct ccgattttg aaagattgt 
      ggcaaagtg gacgacatg agccccaaa tcgaggcaa 
      cagctacaa aatgcgctc gtacaatcg tacgaagac 
      gagttgttg atggttaag tggcagaag tgtcttgat 
      gatggggat ccgaagaaa ttcttcgat gagatttat 
      gtggaaaag tcgagatta atcgaaagt gaggttcct 
      aatgcgtac aatagacat gtcaaggat tttgaaaat 
      ataagcttc acgaaggat ttattcaaa aatgttgat 
      attatcggc caggttgat tgcaaattt cttgcagtg 
      tgggaacgg acgaggaat ttggttgtg ttgtttgac 
      caacatgct gtgcacgag agggtgcgt ttggaggag 
      ctatcggaa ggttacaaa aacgcatcc acgaaactt 
      gaccaagac attacaatt tttttgcca caaagtgac 
      ttaacgctc ctcaagcga caaaaaact aatctcaag 
      tcgctaggt ctcgttttg gagtttttc accaacggg 
      ttgaccatt tatcaagtg ccgctgtgt ctttacaac 
      aagtcgaaa aaaggggtc gatattgtg tttttgact 
      cagactttg atcaaagaa attgttgat cttttgaaa 
      actaacaac gaaattatg gtaaatact ccgagtctt 
      atacaaaat atcgtcaat tccgaggct tgcagaggt 
      gcaattaaa ttcggagat tctttgacc aaaaacgag 
      tgttttgca catttgaaa aatttggca aagtgcaag 
      ttgccgttt caatgcgct cacggaaga ccgacactc 
      actccctta attttcctc aatcgattc tatgatgaa 
      gaggtggca aaaccaaat ttgcacaat ttaagagaa 
      aattga
    mRNA sequence
    • >TC009474 RA
      tttctctaa aatatcgct cattccgtt tggtgttac 
      gctaattat gtttttcaa aaaacaaaa aaccccgga 
      ttttcaaat ttcaaaatg gtggtcgaa ccacttgaa 
      gaaaaagtg attgcgcga attcgaagc tcctcttca 
      attgatagc gtgactcag tgcatcaca gaactggtt 
      ttgaacagt ttggacgcg aaagcgaca gctattgct 
      gttcgagtc aattttaca acttttcgt ctacaagtt 
      gtagacaat gggcgtgga atttcgcgc ccgaattta 
      gaatttgtg ggtcaacgc tacatgacc aacaaatgc 
      cacagcttg gacgacctc cacaaacac ctaaaattc 
      tacggtttc aaaggcgaa gccttgtct aacatttcg 
      cacatttcc caacgtgtg acagttgag actagggaa 
      cacaacagt gaggagact ttcgtcaaa atcttggaa 
      aaagataaa caaacaatc aattcaagt aaatgtcgg 
      gccagccat ggcaccact gtcacagtt gagggtttt 
      ttgcacaac ttaccagtg agacaacaa tgcgtcaaa 
      aacaacgaa ttggaaaat atcaagcgg agccttgag 
      tgccttatt ataatccac ccgcgcgtg tcgttcact 
      ttgcgaaac gatttaggc ctgaaagtt gttctttca 
      agcgttgca acaccagat attgcaagc tccttcaaa 
      acaatccac cctgaaata gacgaaaac gaattcgct 
      ttgctcaaa gttggcaaa aataagaca cgcgttgaa 
      ggtctcatc tacaaacag ttgcacgaa aacaagcgc 
      ctgcagtac ttgtatgtc aataagaga ccggtgaac 
      tcgcccaaa atcctcaat tttgtcaat gcgcttttt 
      aaaaccgtc cccaaaact gatccgaag aggaagaac 
      cccgttttt gtcatacac atcaagtgt ccctactcc 
      gacattgat atcactcta gaaccagca aagacgctt 
      gtgttcttc agaaaaatt gatcttgtg aagcgatgt 
      ctccaaaaa atgatcagt acattcaaa gggggtgat 
      tccgaaata gtggccaag aaaacgcac aggcctttg 
      agtgagttt ggcgtgtcc cagatagga ggggctgtg 
      aaaggcttc gggacgaag cgcaaaagt gaggatttg 
      gaagagttg gtgtcgaat aaatcggcg aaaattgtc 
      gaggatacg ccctatttt gaggaaccg agggttgtg 
      tccaagcaa acgaaggtg gctttgccc attgaggtg 
      aaaaagggg gagaaaagg gagagcgaa ggtataatg 
      gataatagc ctctatacc aattaccct gataacgaa 
      aagaagggg aaagacgtg attatggat atgtttata 
      atgtcactg gacgtgttc ccgcctgaa gataaggct 
      aatgaaact gaacccgaa ctaaataat gtagtggat 
      atggaaact actttagat aaatcacca aatgaaaaa 
      aatgctctt tcccaaaaa tctccgatt aaggagaat 
      atggtttca gtgggtatt caggcagga ccggggaac 
      ataccggta tttggggtc acaccacga aaagctatg 
      tctaaatat gtgtctgac tatgatttt gatttttcc 
      aacatcgat gctttaccg acttttcat tttaaaacg 
      ccagaattg cccacacat tacaatcag ccgaagaaa 
      ttgttcgat tttcgtaat acaaggcgt agcagtttt 
      aatattttt gctaatgaa aatacgttt gatcaagtg 
      acgcaatcg ccgtatttt gctaagaag gccaaagct 
      agcaaattt gagattgat tcgaataaa gagaactgg 
      tcaaatgac ttggataat acagttgat tttaagttt 
      attaaaaaa gacaatacg aaaagaatt ttgaacgaa 
      tcacacact agtaaatac ttcacgcct caaaaatca 
      aagagttgt aaattaagg gttcaaacc ccgaaacac 
      acccttttg gaagagtcg cgagattta tttgataag 
      tcatccgaa agtgggctt ttaataacc ctggatgag 
      gaaattgag aaaaacaaa actgaggat attgtcata 
      gcccccaca tttacgcaa catcagaac ccttggtta 
      aggaaaact ggcaacgac attactttc gaaatgacc 
      gaacgcttt gactttgtc cctaaaggc ctgtctccg 
      attttgaaa gattgtggc aaagtggac gacatgagc 
      cccaaatcg aggcaacag ctacaaaat gcgctcgta 
      caatcgtac gaagacgag ttgttgatg gttaagtgg 
      cagaagtgt cttgatgat ggggatccg aagaaattc 
      ttcgatgag atttatgtg gaaaagtcg agattaatc 
      gaaagtgag gttcctaat gcgtacaat agacatgtc 
      aaggatttt gaaaatata agcttcacg aaggattta 
      ttcaaaaat gttgatatt atcggccag gttgattgc 
      aaatttctt gcagtgtgg gaacggacg aggaatttg 
      gttgtgttg tttgaccaa catgctgtg cacgagagg 
      gtgcgtttg gaggagcta tcggaaggt tacaaaaac 
      gcatccacg aaacttgac caagacatt acaattttt 
      ttgccacaa agtgactta acgctcctc aagcgacaa 
      aaaactaat ctcaagtcg ctaggtctc gttttggag 
      tttttcacc aacgggttg accatttat caagtgccg 
      ctgtgtctt tacaacaag tcgaaaaaa ggggtcgat 
      attgtgttt ttgactcag actttgatc aaagaaatt 
      gttgatctt ttgaaaact aacaacgaa attatggta 
      aatactccg agtcttata caaaatatc gtcaattcc 
      gaggcttgc agaggtgca attaaattc ggagattct 
      ttgaccaaa aacgagtgt tttgcacat ttgaaaaat 
      ttggcaaag tgcaagttg ccgtttcaa tgcgctcac 
      ggaagaccg acactcact cccttaatt ttcctcaat 
      cgattctat gatgaagag gtggcaaaa ccaaatttg 
      cacaattta agagaaaat tgatttttg ttatttaat 
      tatgtatta caagcattt gaataga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY