Status
Please wait ...
Gene information
General information
TC009455 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 908445..916451
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009455
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04635

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009455 RA
      MSIRMKTVL LFLTVLNVV RSEYIPPGP LYRCPKETL 
      LLHPCTCDN EGDQGIYVS CNNTNLATM SIALNNLAT 
      FKLPVEKLT INKCHIARL YGSLLYKLK LRTLLIVDT 
      PIEVIEEHT FLGVNETLN EFHLINSSL KEFPSLAFK 
      ILGNLTELR IDGHNMTDL PKDAFVGGD ISGRLLKLF 
      LPNGYLTTP PIESLQPLR KLKMLDLHG NRITDLKRN 
      QFKGLRDVE ILDLSHNGI RKVDASHLS DLTKLAFFN 
      VSHNNITEL TRGAFARNT ILRVLNMSF NQIKRLDSN 
      TFRGMRFLR RLYLSNNLI TDVGRGTFG SLAQVGTID 
      LARNFLKKI DYQMFFELK FIDTIDVSE NNVTEIQKT 
      AFKDIYLTT INLSRNNIS KIEAGAFQN CANITKLDL 
      SHNRLDTIP KKAFDETTY ALELQLSYN FLTVLNQIP 
      LGNMTGLKI LNVSHNALR TIPKGTFPK LYELHTIDL 
      SYNNLTDIF NSVFQTLFS LRTLDLSHN SLETIKPST 
      FGTLPTLLD LNLSHNFLQ DIARSALTR LASTRNLDV 
      SHNKLKKLF LLPISVSHL DLSFNEFEE LPAKLWPSM 
      NSLLSLDLS HNKLGGGLV EGSFTNLLT LQKLNLNCN 
      GISEPPWAA INELSSLQY LYFEGNNLT NLRRAAFGK 
      LPVVFELNL AHNRISNVS DRAFEGLLQ LIVLNMTNN 
      SISDVPNGA LQGLVALRS LDLSHNKIE KLDNKTHSL 
      FDDCLSLEQ LNLSHNKIS FITRKTFPS NPYVPYKLK 
      EIDLSYNSM PVVTYDLVY GTSKVQKLN LSHNFISDL 
      RKGVIGNLT SLVSLDLSF NRIEDISSD GGVFNLPKS 
      VTEVRLSNN VLHDLPWKK LKNVTKLHI LDIRDNFFE 
      SFGPDLTDM VLKGTEVYF EGNPLNCDC FVRPLKRYF 
      ASQLFLRSF YKFIKCSGP PYLSGQTLF ELPDDRLNC 
      PTNVNTSRI METHPGDYD IAPDLRFRE LSLTQNKVR 
      VKWRVVRND DIADPYVVV RSIKDPGSV VYEATLPYF 
      KRSLEFDLT RPKGSDTEY QICLLARDS KTFIRGFHK 
      EQCRELATS GALAWGARL GVLVMGVVF INRL
    CDS sequence
    • >TC009455 RA
      atgtccatc aggatgaag acagtacta ttatttctc 
      acagttctg aatgtagta aggagtgaa tatattcct 
      ccaggacct ctctacagg tgtcccaag gagactctg 
      ttattgcac ccttgcacg tgtgataat gagggagat 
      caaggaatt tacgttagt tgtaataac acgaatttg 
      gcgacaatg agcatagct ctaaataat ttggctacg 
      tttaaacta ccagttgag aaacttaca ataaacaag 
      tgtcacatc gcccgactt tacggcagt cttttatac 
      aaattaaaa ttgcgaacg ttgttaatt gtcgatact 
      ccgattgaa gtgatagaa gagcacact tttctgggc 
      gtgaatgag acactgaac gagttccac ttgattaat 
      tcaagtctt aaggaattc ccgagtttg gcgttcaag 
      atattgggt aatttaact gagctcaga atagacggt 
      cataacatg acggatttg ccgaaagac gcctttgtt 
      ggaggcgac ataagcggg agactttta aagctattc 
      ttacctaat ggatattta acaactcct cctattgaa 
      agtttacaa ccgttgaga aagctgaaa atgttggat 
      ttgcacggg aatcggatc accgatctg aaaaggaac 
      cagttcaag gggttgcga gatgtggaa attttagat 
      ttgagtcat aatggcatc cgcaaagtg gatgcaagc 
      cacctttcc gatttgacg aagttggcg ttcttcaac 
      gtgtcacat aataatatt acggagctg acgaggggc 
      gcattcgcc agaaacacc attctgcgc gtcctcaac 
      atgtccttc aaccaaatc aagcgcctg gactcgaac 
      actttccgc ggaatgcgt ttcttgcgc cgcctctac 
      ctctccaac aatttaatc acagacgtc ggcagaggc 
      acttttggt tctttggcc caagtcgga acgattgat 
      ctagcgcgc aacttcctc aagaaaatt gattaccaa 
      atgttcttc gaattaaaa ttcattgac acaatcgac 
      gtcagcgag aacaacgtg acggaaatt caaaaaacg 
      gccttcaag gacatttat ctgactacg attaatcta 
      tccagaaac aacatcagt aaaatcgaa gctggggcc 
      ttccagaac tgtgccaac atcacaaag ttggacctt 
      tcccacaac agactggac acgatacca aagaaagcg 
      ttcgatgaa acaacctat gcactagaa ctgcagctt 
      tcgtacaat ttcttaact gttttgaac caaattccg 
      ctgggtaac atgaccggg ctaaaaatc ctcaacgtg 
      tcacataac gcacttagg acgatcccc aaaggaaca 
      ttcccaaaa ctctacgag ttgcacacg attgacttg 
      tcgtacaac aatctaact gatattttt aattccgtt 
      tttcaaact ttattttcc ttgcgaacg cttgatttg 
      agccacaac agcctcgaa accatcaaa cccagcact 
      tttgggacc cttcccacc ttactggac ctaaatctg 
      agccacaac tttttgcaa gacatcgca cgaagtgct 
      ctaacacgc cttgccagc accaggaac ctggacgtc 
      agccacaac aagctcaaa aaactgttc ctccttccc 
      atttcagtg tcacactta gacttgtct ttcaacgag 
      tttgaggaa ctcccagcc aagctgtgg cccagtatg 
      aactcactt ttgagtctt gatttgagt cataataag 
      cttggtggt ggtttggtt gaagggagt ttcacaaat 
      ttgctaacg ttgcaaaaa ctcaatttg aattgtaat 
      gggatttcg gaacctcca tgggctgcg atcaacgag 
      ctaagttcg ttgcaatat ttgtatttt gagggtaat 
      aatttaaca aacttgaga cgagctgct tttggaaaa 
      ttgccagtt gttttcgaa ctcaatttg gctcataat 
      cggattagc aatgtcagt gatcgggcc tttgagggg 
      ctcttgcag ctgattgtg ctgaatatg accaataat 
      agcataagt gatgttccg aatggggcc cttcaaggc 
      ctggtggcc ttacggagc ttggatctt tcgcacaat 
      aaaatcgag aagctagat aacaaaacg cacagttta 
      tttgatgac tgcttatct ctggagcaa ctgaatttg 
      agccacaac aaaattagc ttcataacg cgcaaaact 
      ttccccagt aatccttac gtcccgtac aaactgaaa 
      gaaatcgat ctgtcgtac aattcgatg ccggttgtt 
      acctacgat ctagtttac ggaacctcc aaagtgcag 
      aaactcaac ttgtcgcat aatttcatt tcggatctg 
      cggaaaggt gttattgga aacttgact agtttagtg 
      tcgttggac ttgtccttt aacagaatc gaagacatt 
      tcaagtgat ggtggggtt tttaatttg cccaaaagt 
      gtgacagaa gtgcgattg tccaacaat gtcttgcac 
      gacttgcct tggaaaaag ctcaaaaat gtgactaaa 
      ctgcacatt ttagacatt agagacaac ttttttgaa 
      agttttggg cccgacttg accgacatg gtccttaaa 
      ggcactgaa gtttatttc gaaggaaat ccgctaaat 
      tgcgactgc tttgtgcgt cctttgaaa cgctacttc 
      gcctctcag ctgttcctg cgctccttt tacaagttt 
      ataaagtgt tcgggtccc ccttacctg tcaggccag 
      acattattc gagctcccc gatgacagg ctaaattgc 
      cctactaat gttaatact tcaaggata atggagacg 
      catcctgga gactatgat atagcgccc gatttgcgg 
      ttccgggag ctcagcctt acgcagaac aaagtgagg 
      gtgaaatgg cgagtggtg aggaacgac gatatagcc 
      gatccttac gttgtcgtc aggagcata aaggatcct 
      gggagcgtc gtctatgaa gcaactttg ccgtatttt 
      aaaagaagt ttagagttc gacctgact cgcccgaag 
      gggagcgat actgaatat caaatatgt ttgctagcg 
      agggacagc aaaactttt atacgtggc ttccacaaa 
      gaacaatgc agggagttg gcgacgagc ggagcgctg 
      gcctgggga gcaaggctt ggggttctt gtgatggga 
      gttgtgttt ataaataga ctgtga
    mRNA sequence
    • >TC009455 RA
      tggcaatca aaaataacc aatcacaaa tattaaaaa 
      aacaactgc tacttcaac tgtgcttta tttctccgt 
      ttgagttta atttcacac aaaacccag acatgcttg 
      cttttcagc ttgtgtccc agaaatttc cataaacat 
      ttccgctcg tgttttttt atcttgcac cctttgcca 
      ataaaacta cagccgtat ttttggttt ttgcaagga 
      atgaatagt ttatcgtag attctctcg aggataaaa 
      catcgccga ttcataaat ttccccgaa cactgagga 
      cagtgcggt ttgactctc tcgaaacaa gcgggacat 
      aaactgtgt ccatttgtg caaacttgg atgggtgga 
      gaaagatac gatggacaa atggagcac aacaacgcc 
      gaaagtaac tcttgatca gacatttga cattttaaa 
      agcgccaac taagcgtga aaaacaatt ctcttaatg 
      cagtttcca ttagttgat tcgtcaata gtttgtttt 
      tattcaatt atcttaaaa agtatgtct ttggcggaa 
      gtttcatga ccctcctcc tcttagtca cattccgct 
      ggcttacct tgagtaagg taaaagtta aatgttaca 
      agacttgaa actacaaat tctcgcaca aaatgggtg 
      ttaaaagtg tctaataat ctcaaaatg tccatcagg 
      atgaagaca gtactatta tttctcaca gttctgaat 
      gtagtaagg agtgaatat attcctcca ggacctctc 
      tacaggtgt cccaaggag actctgtta ttgcaccct 
      tgcacgtgt gataatgag ggagatcaa ggaatttac 
      gttagttgt aataacacg aatttggcg acaatgagc 
      atagctcta aataatttg gctacgttt aaactacca 
      gttgagaaa cttacaata aacaagtgt cacatcgcc 
      cgactttac ggcagtctt ttatacaaa ttaaaattg 
      cgaacgttg ttaattgtc gatactccg attgaagtg 
      atagaagag cacactttt ctgggcgtg aatgagaca 
      ctgaacgag ttccacttg attaattca agtcttaag 
      gaattcccg agtttggcg ttcaagata ttgggtaat 
      ttaactgag ctcagaata gacggtcat aacatgacg 
      gatttgccg aaagacgcc tttgttgga ggcgacata 
      agcgggaga cttttaaag ctattctta cctaatgga 
      tatttaaca actcctcct attgaaagt ttacaaccg 
      ttgagaaag ctgaaaatg ttggatttg cacgggaat 
      cggatcacc gatctgaaa aggaaccag ttcaagggg 
      ttgcgagat gtggaaatt ttagatttg agtcataat 
      ggcatccgc aaagtggat gcaagccac ctttccgat 
      ttgacgaag ttggcgttc ttcaacgtg tcacataat 
      aatattacg gagctgacg aggggcgca ttcgccaga 
      aacaccatt ctgcgcgtc ctcaacatg tccttcaac 
      caaatcaag cgcctggac tcgaacact ttccgcgga 
      atgcgtttc ttgcgccgc ctctacctc tccaacaat 
      ttaatcaca gacgtcggc agaggcact tttggttct 
      ttggcccaa gtcggaacg attgatcta gcgcgcaac 
      ttcctcaag aaaattgat taccaaatg ttcttcgaa 
      ttaaaattc attgacaca atcgacgtc agcgagaac 
      aacgtgacg gaaattcaa aaaacggcc ttcaaggac 
      atttatctg actacgatt aatctatcc agaaacaac 
      atcagtaaa atcgaagct ggggccttc cagaactgt 
      gccaacatc acaaagttg gacctttcc cacaacaga 
      ctggacacg ataccaaag aaagcgttc gatgaaaca 
      acctatgca ctagaactg cagctttcg tacaatttc 
      ttaactgtt ttgaaccaa attccgctg ggtaacatg 
      accgggcta aaaatcctc aacgtgtca cataacgca 
      cttaggacg atccccaaa ggaacattc ccaaaactc 
      tacgagttg cacacgatt gacttgtcg tacaacaat 
      ctaactgat atttttaat tccgttttt caaacttta 
      ttttccttg cgaacgctt gatttgagc cacaacagc 
      ctcgaaacc atcaaaccc agcactttt gggaccctt 
      cccacctta ctggaccta aatctgagc cacaacttt 
      ttgcaagac atcgcacga agtgctcta acacgcctt 
      gccagcacc aggaacctg gacgtcagc cacaacaag 
      ctcaaaaaa ctgttcctc cttcccatt tcagtgtca 
      cacttagac ttgtctttc aacgagttt gaggaactc 
      ccagccaag ctgtggccc agtatgaac tcacttttg 
      agtcttgat ttgagtcat aataagctt ggtggtggt 
      ttggttgaa gggagtttc acaaatttg ctaacgttg 
      caaaaactc aatttgaat tgtaatggg atttcggaa 
      cctccatgg gctgcgatc aacgagcta agttcgttg 
      caatatttg tattttgag ggtaataat ttaacaaac 
      ttgagacga gctgctttt ggaaaattg ccagttgtt 
      ttcgaactc aatttggct cataatcgg attagcaat 
      gtcagtgat cgggccttt gaggggctc ttgcagctg 
      attgtgctg aatatgacc aataatagc ataagtgat 
      gttccgaat ggggccctt caaggcctg gtggcctta 
      cggagcttg gatctttcg cacaataaa atcgagaag 
      ctagataac aaaacgcac agtttattt gatgactgc 
      ttatctctg gagcaactg aatttgagc cacaacaaa 
      attagcttc ataacgcgc aaaactttc cccagtaat 
      ccttacgtc ccgtacaaa ctgaaagaa atcgatctg 
      tcgtacaat tcgatgccg gttgttacc tacgatcta 
      gtttacgga acctccaaa gtgcagaaa ctcaacttg 
      tcgcataat ttcatttcg gatctgcgg aaaggtgtt 
      attggaaac ttgactagt ttagtgtcg ttggacttg 
      tcctttaac agaatcgaa gacatttca agtgatggt 
      ggggttttt aatttgccc aaaagtgtg acagaagtg 
      cgattgtcc aacaatgtc ttgcacgac ttgccttgg 
      aaaaagctc aaaaatgtg actaaactg cacatttta 
      gacattaga gacaacttt tttgaaagt tttgggccc 
      gacttgacc gacatggtc cttaaaggc actgaagtt 
      tatttcgaa ggaaatccg ctaaattgc gactgcttt 
      gtgcgtcct ttgaaacgc tacttcgcc tctcagctg 
      ttcctgcgc tccttttac aagtttata aagtgttcg 
      ggtccccct tacctgtca ggccagaca ttattcgag 
      ctccccgat gacaggcta aattgccct actaatgtt 
      aatacttca aggataatg gagacgcat cctggagac 
      tatgatata gcgcccgat ttgcggttc cgggagctc 
      agccttacg cagaacaaa gtgagggtg aaatggcga 
      gtggtgagg aacgacgat atagccgat ccttacgtt 
      gtcgtcagg agcataaag gatcctggg agcgtcgtc 
      tatgaagca actttgccg tattttaaa agaagttta 
      gagttcgac ctgactcgc ccgaagggg agcgatact 
      gaatatcaa atatgtttg ctagcgagg gacagcaaa 
      acttttata cgtggcttc cacaaagaa caatgcagg 
      gagttggcg acgagcgga gcgctggcc tggggagca 
      aggcttggg gttcttgtg atgggagtt gtgtttata 
      aatagactg tgaaattgt agaaagctt gtaaatatt 
      gttgaataa aagttgttt taccag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY